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이민구,이상길,남경수 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.6
Ginkgolide B (GKB) is a natural terpenoid lactone found in leaves of Ginkgo biloba and has antioxidative, anti-inflammatory, and anti-tumor activities. Here, we assessed the inhibitory effect of GKB on the 12-Otetradecanoylphorbol 13-acetate (TPA)-induced metastatic potential in MCF-7 cells (a human hormone receptor-positive breast cancer cell line). Gelatin zymography showed that GKB inhibited TPA-induced matrix metalloprotease-9 (MMP-9) activity, and wound healing and Matrigel invasion assays showed GKB inhibited TPA-induced cell migration and invasiveness. Furthermore, GKB suppressed the TPAinduced mRNA expressions of epithelial to mesenchymal transition (EMT) markers Slug, Snail, and Fibronectin genes. Based on these results, we assessed the effects of GKB on the phosphorylation of mitogen-activated protein kinases (MAPKs) and the expressions of c-Jun and c-Fos (two subunits of activating protein-1 [AP-1] and key regulators of the expressions of metastatic factors). GKB inhibited the phosphorylations of p38 (p38 mitogen-activated protein kinases) and JNK (c-Jun N-terminal kinase) and the expressions and nuclear translocations of c-Jun and c-Fos. These results demonstrate that the anti-metastatic effect of GKB is mediated by MAPK/AP-1 pathways suppression and consequent inhibitions of the MMP-9 activities and mRNA levels of EMT markers in TPA-treated MCF-7 cells. Our results suggest that GKB might be able to suppress metastasis in hormone receptor-positive breast cancer.
역행성 동맥 혈류를 이용한 원위 유리피판술의 실험적 연구
이민구,민경원,Lee, Min-Goo,Minn, Kyung-Won 대한미세수술학회 1998 Archives of reconstructive microsurgery Vol.7 No.1
Microsurgical free-tissue transfer has allowed surgeons to salvage injured limbs but choosing appropriate healthy recipient vessels has proved to be a difficult problem. Retrograde flow flaps are established in island flaps. Retrograde flow anastomosis could prevent the possible kinking and twisting of the arterial anastomosis. By not interrupting the proximal blood flow to the fracture or soft tissue defect site, the compromise of fracture or wound healing might be prevented. We wished to estabilish an animal model in rat for a retrograde arterial flow based free flap. Nembutal-anesthetized male rats; weighing 250 to 300 gm, were used. The femoral artery and common carotid artery were exposed and divided. The systemic and retrograde arterial pressure were quantified by utilizing a parallel tubing system connected with peripheral arterial line. In this study, the retrograde flow was not pulsatile and the retrograde arterial pressure was 64-65mmHg, with a mean arterial pressure of 106-109mmHg. An epigastiic skin flap, measuring $3{\times}3cm$, was raised with its vascular pedicle. The epigastric free flap was transfered in the same rat from femoral vessels to carotid vessels in end to end fashion. We anastomosed the donor arteries to the distal parts of the divided recipient arteries and the donor veins to the proximal parts of the recipient veins. Twelve experiments were performed and the transplantations succeeded in 75 percent of them. In the remaining 25 percent, the experiments failed due to thrombosis at the site of anastpmosis, or other causes. This animal model represents an excellent example of retrograde arterial flow free flap transfer that is reliable.
Bottom-ash를 이용한 이산화탄소 전환을 위한 기초 연구
이민구,강동우,박상원,조호용,박진원 한국폐기물자원순환학회 2014 젊은 연구자 학술연구발표회 Vol.13 No.0
이산화탄소를 포집한 후 저장 공간이 부족하며, 환경파괴의 문제점이 제시되면서 저장의 한계성을 보이고 있다. 온실가스인 이산화탄소와 폐기물을 이용하여 자원화를 시키는 것은 매우 진보된 기술이다. 본 연구에서는 Bottom-ash(from Petroleum Coke)의 Ca<sup>++</sup> 이온을 추출하여 이산화탄소를 흡수시킨 MEA 용액과 반응하여 CaCO<sub>3</sub>를 얻는 연구를 수행하였다. 흡수제는 5 wt% 농도의 MEA를 사용하여 진행하였으며 이온 추출을 위한 HCl은 0.1 M, 0.5 M, 1.0 M로 시간에 따라 각각 진행하였다. 이온추출은 HCl의 농도에 따라 바뀌는 것을 알 수 있었으며 시간에는 영향이 없는 것으로 나타났다. 또한 이산화탄소를 포함한 흡수제와 침전 실험을 진행한 결과 90 % 수율을 보였다. 침전된 탄산칼슘은 XRD 분석 결과 Calcite 형태로 존재함을 확인 할 수 있다.
민경원,고경호,이민구 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5
The clinical applications of free have been extended tremendously in head and neck reconstruction. In a nine-year period, 92 microvascular free flaps were performed to repair the defects following major head and neck ablative surgery. Twenty-one transverse rectus abdominis myocutaneous flaps, 18 radial foream flaps, 15 latissimus dorsi muscle flaps, 12 jejunal flaps, 8 fibular osteocutaneous flaps, 6 rectus abdominis muscle flaps, 6 iliac osteomyocutaneous flaps, 2 groin fasciocutaneous flaps, 1 scapular fasciocutaneous flap, 1 parascapular osteocutaneous flap, 1 tensor fascia lata muscle flap and 1 serratus anterior muscle flap were used for reconstruction. Twenty-five maxillary defects including the orbit or skull base, 16 pharyngoesophageal defects, 15 intraoral defects, 15 mandibular defects, 13 scalp defects, 1 cervical region and 7 other facial region were covered with various free flaps. The overall success rate of the flaps was 95.6%. The complications included total flap loss(3 cases), partial flap loss(1 case), recurrence of primary tumors(15 cases), cerebrospinal fluid leakage(3 cases), fistula formation(3 cases) and infection(5 cases). Superficial temporal artery, facial artery, superior thyroidal artery, lingual artery, occipital artery, transverse cervical artery were commonly used recipient arteries and 7 cases of vein grafts were used if indicated. End to end anastomosis was performed in 84 cases and end to side anastomosis in 8 cases. The average follow-up period was 42 months, ranging from 6 months to 8 years. One patent died during postoperative intensive care due to sepsis and 19 patients died because of recurrence of tumors and underlying medical diseases during the follow-up period. Although free flaps may appear to be riskier than traditional forms of reconstruction, they offer the surgeon a greater spectrum of reconstructive options. Free flap reconstruction also improves the quality of life and minimizes the loss of function. Limitations of the use of free flaps result only from a lack of technical skills and specialized equipment.