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간호대학생의 생명의료윤리 의식, 도덕적민감성, 사전연명의료의향서 지식 및 태도의 관계연구
김지현,노지원,현지민,김봉호,최민혁,김정은,최순호,나지현,김세인 대전대학교 기초과학연구소 2021 自然科學 Vol.32 No.-
Purpose: The purpose of this study was to identify the relationships among nursing students’ consciousness of biomedical ethics, moral sensitivity, knowledge and attitudes to advance medical directives. Methods: the study was a cross-sectional and descriptive survey, carried out with 137 students from two schools of nursing, one located in D city. Student nurses' consciousness of biomedical ethics, moral sensitivity, knowledge and attitudes to advance medical directives. were measured using structured self-report questionnaires. data were analyzed with independent t-test, one-way ANOVA and Pearson’s correlation coefficients using SP SS/WIN 22.0 program. Results: The scores for knowledge, attitudes for medical advance directives were 7.86 and 4.00 and for ethics values, 3.47. Moral sensitivity had a significant positive correlation with attitudes toward medical advance directives(r=.192, p=.025) and knowledge toward medical advance directives(r=.286, p=.001). Nursing students’ Moral sensitivity, knowledge and attitude showed a significant difference depending on grade. Conclusion: The results of this study indicate that basic for the development of an effective education program on ethics is important in establishing a positive attitude toward advance medical directives and the appropriate ethical values in student nurses.
최선실,이상권,김중관,박혜경,이유진,장진호,이요한,김건우,현지민,엄혜진,이세민,Kang Byuong Heon,채영찬,명경재,명승재,박찬영,최장현 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-
The endoplasmic reticulum (ER) stress response is an adaptive mechanism that is activated upon disruption of ER homeostasis and protects the cells against certain harmful environmental stimuli. However, critical and prolonged cell stress triggers cell death. In this study, we demonstrate that Flightless-1 (FliI) regulates ER stress-induced apoptosis in colon cancer cells by modulating Ca2+ homeostasis. FliI was highly expressed in both colon cell lines and colorectal cancer mouse models. In a mouse xenograft model using CT26 mouse colorectal cancer cells, tumor formation was slowed due to elevated levels of apoptosis in FliI-knockdown (FliI-KD) cells. FliI-KD cells treated with ER stress inducers, thapsigargin (TG), and tunicamycin exhibited activation of the unfolded protein response (UPR) and induction of UPRrelated gene expression, which eventually triggered apoptosis. FliI-KD increased the intracellular Ca2+ concentration, and this upregulation was caused by accelerated ER-to-cytosolic efflux of Ca2+. The increase in intracellular Ca2+ concentration was significantly blocked by dantrolene and tetracaine, inhibitors of ryanodine receptors (RyRs). Dantrolene inhibited TG-induced ER stress and decreased the rate of apoptosis in FliI-KD CT26 cells. Finally, we found that knockdown of FliI decreased the levels of sorcin and ER Ca2+ and that TG-induced ER stress was recovered by overexpression of sorcin in FliI-KD cells. Taken together, these results suggest that FliI regulates sorcin expression, which modulates Ca2+ homeostasis in the ER through RyRs. Our findings reveal a novel mechanism by which FliI influences Ca2+ homeostasis and cell survival during ER stress.