개의 외인성 에스트로겐 중독 증례

한재익,김태훈,이숙진,강지훈,조상희,장동우,양만표,나기정 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.3

e Veterinary Medical Center of Chungbuk NationalUniversity due to anorexia, depression and weight loss after the administration of exogenous estrogen for contraceptionin the local hospital. Although the dog was medicated with antibiotics and fluid for two weeks in the local hospital,there was a deterioration in the patient's general condition. Therefore the dog was referred to our Veterinary MedicalCenter. Hematological and biochemical analysis showed non-regenerative anemia, thrombocytopenia, severely immatureleukocytosis, hyperbilirubinemia, hyperglycemia and elevated serum alkaline phosphatase, gamma-glutamyltransferase,and lactate dehydrogenase. Abdominal ultrasonography showed double line of gallbladder and irregular hypertrophicwall of uterus. On the basis of these examination's results and history taking, final diagnosis was exogenous estrogen-induced myelotoxicosis with cholecystitis and endometritis. The symptomatic therapy for estrogen toxicosis wasperformed for 26 days after the final diagnosis. However,and anemia. On day 135 after administration of estrogen, platelet and neutrophil count were normalized, but monocyticleukocytosis was occurred. A 2-year-old female mongrel dog was referred to the Veterinary Medical Center of Chungbuk National University due to anorexia, depression and weight loss after the administration of exogenous estrogen for contraception in the local hospital. Although the dog was medicated with antibiotics and fluid for two weeks in the local hospital, there was a deterioration in the patient's general condition. Therefore the dog was referred to our Veterinary Medical Center. Hematological and biochemical analysis showed non-regenerative anemia, thrombocytopenia, severely immature leukocytosis, hyperbilirubinemia, hyperglycemia and elevated serum alkaline phosphatase, gamma-glutamyltransferase, and lactate dehydrogenase. Abdominal ultrasonography showed double line of gallbladder and irregular hypertrophic wall of uterus. On the basis of these examination's results and history taking, final diagnosis was exogenous estrogeninduced myelotoxicosis with cholecystitis and endometritis. The symptomatic therapy for estrogen toxicosis was performed for 26 days after the final diagnosis. However, the symptom had no response to therapy except endometritis and anemia. On day 135 after administration of estrogen, platelet and neutrophil count were normalized, but monocytic leukocytosis was occurred.

Gizzard Impaction with Bile Stasis in Captive Oriental White Storks (Ciconia boyciana)

한재익,손형원,모인필,장동우,김수경,박시룡,나기정 한국임상수의학회 2009 한국임상수의학회지 Vol.26 No.6

The Korea Institute of Oriental White Stork Rehabilitation Research (KIOWSRR) is breeding oriental white storks for residential rehabilitation in South Korea. Three oriental white storks were referred with anorexia and depression. Two storks were died before examinations and one stork was died after 1 day of fluid therapy. On necropsy, salmon-colored subcutaneous and visceral fats were examined in all storks. Multiple focal necroses on liver surface were also examined. In gizzards, many gravels and grasses were severely tangled; therefore, it produced a large mass that blocked digestive system of the storks. Small intestinal segments of the stork, which was dead after the fluid therapy, were severely adhesive. On histopathology, diffuse necroses with/without inflammation were examined in the liver, gizzard and intestine in all storks. Bile stasis was additionally examined in the liver. According to the results of the examinations, all storks were diagnosed as gizzard impaction caused by parorexia.

Molecular Detection of Haemoproteus in Two Wild Eurasian Eagle Owls (Bubo bubo) in Middle Area of South Korea

한재익,장혜진,이종원,강효민,나기정 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

This report describes asymptomatic Haemoproteus infection in wild Eurasian eagle owls (Bubo bubo) diagnosed by blood smear and DNA analysis. This is the first description of natural Haemoproteus infection in wild Eurasian eagle owl in South Korea and suggests that the wild resident bird population can be a source for Haemoproteus infection of domestic poultry.

닥스훈트 개의 특발성 다발성 결절 지방층염 증례

한재익,장동우,김근형,나기정 한국임상수의학회 2008 한국임상수의학회지 Vol.25 No.5

A 2-year-old intact female dachshund dog was presented with recurrent subcutaneous nodules and fistulations on the neck, back and hip. The patient was diagnosed as a sterile nodular panniculitis based on the cytology, histopathology, and cultures for bacteria and fungus. The fistulas were surgically removed and methylprednisolone was administrated 2 mg/kg twice daily per oral. The lesions were all disappeared, but the relapses were happened when the dosage of the drug was tapered off. The repeated treatments with methylprednisolone or azathioprine were performed, consequently, the patient was controlled with relatively low-dosage methylprednisolone (0.25 mg/kg, every third day).

Suspected Unilateral Renal Dysplasia in a Cat with Intermittent Hematuria

한재익,나기정 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.1

A 6-month-old, castrated male, Korean short-haired cat was referred with intermittent hematuria. Physical and laboratory examination including complete blood count and serum biochemistry profiles showed no specific findings. Ultrasonography revealed a cystic lesion of the mid-abdomen, which was approximately 2 × 2 cm in size. FNA cytology of cystic fluid showed numerous cholesterol crystals of varying size and shape and granulomatous inflammation. CT examination showed fluid-filled large cystic lesion in the left side of the abdomen. Taken together, this case was diagnosed as unilateral (left) renal dysplasia and cystic inflammation led by cholesterol crystallization in the left kidney.

Increased beta-globulin Levels in Captive Oriental White Storks Fed a Diet of Sand Eels

한재익,장혜진,정석환,김수경,박시룡,Barry K. Hartup,나기정 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.3

The aim of the study was to identify the effects of two different diets (sand eels or saurels) on the serumprotein electrophoretic patterns of Oriental White Storks held in captivity. The tests were performed on two groupsof storks according to the diet (group 1 and 2). Twenty-two (group 1) or twenty-nine (group 2) storks were included. The values of complete blood count (CBC), serum biochemistry profiles, protein fractions (albumin, α-globulin, β-globulin, and γ-globulin), and lipoprotein (high density- [HDL] and low density lipoprotein [LDL]) were comparedbetween samples obtained during two groups (p < 0.05). The α-globulin fraction was decreased and the β-globulinfraction was significantly increased in samples obtained from group 1 compared to those obtained from group 2. Ingroup 1, the concentration of LDL was also significantly increased compared to that of group 2. In conclusion, weconfirmed that the β-globulin fraction was significantly elevated in storks fed sand eels.

Multiplex Quantitative Real-time Polymerase Chain Reaction Assay for Rapid Detection of Mycobacterium avium subsp. paratuberculosis in Fecal Samples

한재익,정영훈,최창용,유재규,강석진,유한상,박흥태,권응기,조용일 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

Mycobacterium avium subsp. paratuberculosis (MAP) causes paratuberculosis or Johne's disease, an intestinal granulomatous infection in domestic and wild animals. The study aimed to develop and evaluate a panel of multiplex quantitative real-time polymerase chain reaction (mqPCR) assay for simultaneous detection of three MAP-specific genes (IS900, F57 and ISMAP02 genes). The analytic sensitivity (i.e., limit of detection, expressed as cells per 1 ml) was 150 for IS900, 1500 for F57, and 50 for ISMAP02. The specificity of the method was determined by testing 152 bovine fecal samples. Based on the test, it showed that the assay simultaneously detected the target genes in short period of time and at lower cost compared to laboratory routine tests. The test agreement between the assay and routine test was 94%. The discrepancy in the results was due to samples that were tested positive by the panel but negative by the routine tests, suggesting that the assay has higher sensitivity than the routine tests. In conclusion, the mqPCR assay could be a rapid and accurate testing tool for investigating paratuberculosis or Johne's disease cases in domestic and wild animals.

Enteritis Caused by Type A Clostridium perfringens Producing α-Toxin in a Dog

한재익,장혜진,천행복,김지혜,김근형,장동우,나기정 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.2

A 6-year-old, female, Siberian husky was referred with mucous diarrhea. On fecal examination, numerous clustered and individual large epithelial cells and rod-shaped, spore-forming bacteria were examined. By bacterial culture and molecular typing, the bacteria was identified as Clostridium perfringens (C. perfringens), and by toxin analysis of C. perfringens, production of α-toxin was confirmed. Based on these results, the dog was diagnosed as enteritis caused by C. perfringens producing α-toxin, and was treated with amoxicillin/clavulanate. After 1 week, the diarrhea was disappeared and no spore-forming bacteria were examined on fecal examination. This report shows that the rapid and exact diagnosis keeps a effective treatment for enteritis caused by C. perfringens producing α-toxin in dogs.

Pulmonary Candidiasis in a Dog Receiving Long-term Management for Atopic Dermatitis

한재익,이창민,김태우,이인,최수영,박희명 한국임상수의학회 2015 한국임상수의학회지 Vol.32 No.3

A canine patient exhibited partial anorexia and sudden respiratory distress. Diagnostic imaging and cytology of tracheal-lavage fluids revealed fungal pneumonia. Molecular identification and phylogenetic analysis detected Candida catenulata. Treatment with oral itraconazole for 3 weeks was effective. This is the first report of C. catenulata infection in a dog.

High Prevalence of Lipid-Dependent Malassezia Infections in Dogs

한재익,조상희,나기정 한국임상수의학회 2010 한국임상수의학회지 Vol.27 No.2

Malassezia (M.) is a member of normal mycobiota in warm-blooded vertebrates. Increased humidity is likely to be crucial in this infection. We studied the proportion of the species infected in dog during summer of Korea. Fifty samples were analyzed by PCR-RFLP and direct sequencing from June 2006 to October 2006. The study showed that lipid-dependent species was main pathogen (M. furfur; 86%, M. obtusa; 10%) while M. pachydermatis (4%) has only small portion. This result suggests that Malassezia infection has endemic characters that can be affected by the climate (temperature and humidity) in dogs.