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최창용,김홍건 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.6
A precise measurement of field test was performed to estimate the thermal performance of the forced convection electric air heater by experiment. air temperature, flow rate and electrical power input were measured with the related measurement sensors, and acquisition methods for the measured data were studied to estimate the thermal performance of the tested air heater effectively. To determine the mean air temperature at the flow cross-section, measuring positions were chosen by considering the flow velocity profile and the equally divided cross-sectional area. From the experimental results, thermal efficiency was obtained accurately as an indication of the tested heating system performance.
과산화수소와 동결에 의해 유도된 생쥐와 소 수정란의 사멸에 있어서 칼륨 통로의 역할
최창용,김창운,강다원,한재희 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.3
Programmed cell death or apoptosis is associated with changes in K+ concentration in many cell types. Recentstudies have demonstrated that two-pore domain K+ (K2P) channels are involved in mouse embryonic development andapoptotic volume decrease of mammalian cells. In cerebellar granule neurons that normally undergo apoptosis duringthe early developmental stage, TASK-1 and TASK-3, members of K2P channels, were found to be critical for celldeath. This study was performed to identify the role of K+ channels in the H2O2-induced or cryo-induced cell deathof mouse and bovine embryos. Mouse and bovine two-cell stage embryos (2-cells) exposed to H2O2 for 4 h sufferedfrom apoptosis. The 2-cells showed positive TUNEL staining. Treatment with high concentration of KCl (25mM)inhibited H2O2-induced apoptosis of 2-cells by 19%. Cryo-induced death in bovine blastocysts showed positive TUNELstaining only in the cells near the plasma membrane. Cryoprotectant supplemented with 25 mM KCl reduced apoptosisslightly compared to cryoprotectant supplemented with 5 mM KCl. However, the combination of antioxidants (β-mercaptoethanol) with 25 mM KCl significantly decreased the rate of H2O2-induced and cryo-induced apoptosiscompared to treatments with only antioxidants or 25 mM KCl. These results show that blockage of K+ channel effluxfor a short-time reduces H2O2- and cryo-induced apoptosis in mouse and bovine embryos. Our findings suggest thatapoptosis in mouse and bovine embryos might be controlled by modulation of K+ channels which are highly expressedin a given cell type.