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신미옥,지다정,강소영,양원경,최화경,이수연 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.6
A recent trend in urine drug testing in forensicand clinical toxicology has been the simultaneous determinationof different chemical groups of target drugs, which areselected based on their local popularity. Rapid multiple druganalysis, made possible by the use of liquid chromatography–tandem mass spectrometry (LC/MS/MS), has becomemore widely used, especially in workplace drug testing. Therefore, in the present study, a method for simultaneouslyanalyzing 35 drugs of abuse and relevant metabolites that aremost prevalent in Korea, using LC/MS/MS with polarityswitching electrospray ionization, was developed and validated. The drugs and metabolites in urine were extracted byusing mixed mode strong cation exchange polymeric solidphase extraction cartridges after enzymatic hydrolysis andwere then injected into the LC/MS/MS system. The validationresults for selectivity, linearity, intra- and inter-assayprecision and accuracy for this method were satisfactory,while the results for matrix effects and recovery showedsignificant variance among the urine samples from differentsources. The limits of detection ranged from 0.1 to 10 ng/ml and the limits of quantification were from 1 to 10 ng/ml. Toreduce the matrix effects in authentic samples, two differentquantitative approaches were compared: quantificationusing calibration standards prepared by the drug-free pooledurine matrix and quantification using the standard addition. Of these, the latter method was found to be the most suitable. The method developed in this study will be very useful forforensic and clinical toxicology laboratories to adopt formonitoring the inappropriate use of controlled drugs.