나이관련 황반변성에 대한 동서의학적 고찰

정유진,고우신,윤화정,Jung, Yu-Jin,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.3

Object : The purpose of this study is to understand age-related maculardegeneration(AMD) with both western and Korean medicine.Methods : We investigated the comprehension of general AMD degenation in both western and Korean medicine through literature review.Results : The results are as follows.1. AMD prevalent increasing as the population ages; however, treatment options remain limited and incompletely defined.2. Generally macular degeneration has been affected by aging and is associate with the function of kidney(腎) and liver(肝) in Korean medicine.Conclusion : Further studies are needed to apply comprehension of AMD in Korean medicine to clinical stage.

박탈성 피부염의 한방 치험 1례

정유진,윤화정,고우신,Jung, Yu-Jin,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.3

Objective : The purpose of this report is to know the effect of Korean medical treatments on exfloliative dermatitis. Methods : We treated a 25-years-old man patient with exfloliative dermatitis on the limbs and back with Korean medicine. After Korean medical teatment for 26 days, we measured the decrease of dermatitis and recovery of skin exfloliate. We recorded pictures of changes on symptoms. Result & Conclusion : The symptoms of exfloliative dermatitis were significantly improved. The dry skin and itching were disappeared and skin damages were almost recovered. Thus Korean medical treatments are effective on exfloliative dermatitis.

유연성 다중 회귀 모델을 활용한 보행자 이상 행동 예측 모델 연구

정유진,윤용익,Jung, Yu Jin,Yoon, Yong Ik 한국데이터정보과학회 2016 한국데이터정보과학회지 Vol.27 No.1

최근 강력 범죄 및 우발 범죄가 끊이지 않고 있으면서 사회적 불안감이 고조되고 있다. 이에 따라 방범용 카메라, CCTV (Closed Circuit Television)가 범죄 증거 확보와 치안을 위해 사용되고 있다. 그러나 CCTV는 주로 사후 처리 기능으로 사용하고 있으며 사전에 범죄를 예방하기는 힘들다. 본 연구에서는 CCTV로부터 수집된 보행자 데이터를 이용하여 객체의 행동을 분석하고 위험 행동 여부를 추정하기 위한 유연성 다중 회귀 모델을 제안한다. 유연성 다중 회귀 모델은 필터링, 상황분석, 예측 단계로 구성되어 있다. 먼저 보행자에 대한 환경과 상황에 대해 필터링한 후 상황분석에 대한 정보를 구축하고 관찰 객체에 이상 행동이 결정된다. 마지막으로 연관분석을 통해 객체의 행동이 예측되어 위협 상황을 통지한다. 이를 통해 다중 지역에서 객체의 행동을 추적하여 객체 행동의 위험여부를 알 수 있으며, 행동 예측을 통해 범죄 발생을 예측 가능하다. In the recently, violent crime and accidental crime has been generated continuously. Consequently, people anxiety has been heightened. The Closed Circuit Television (CCTV) has been used to ensure the security and evidence for the crimes. However, the video captured from CCTV has being used in the post-processing to apply to the evidence. In this paper, we propose a flexible multi-level models for estimating whether dangerous behavior and the environment and context for pedestrians. The situation analysis builds the knowledge for the pedestrians tracking. Finally, the decision step decides and notifies the threat situation when the behavior observed object is determined to abnormal behavior. Thereby, tracking the behavior of objects in a multi-region, it can be seen that the risk of the object behavior. It can be predicted by the behavior prediction of crime.

벼에서 CRISPR/Cas9 활용 고빈도 유전자 편집 방법

정유진,배상수,이긍주,서필준,조용구,강권규,Jung, Yu Jin,Bae, Sangsu,Lee, Geung-Joo,Seo, Pil Joon,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1

CRISPR/Cas9 기술은 생명공학을 활용한 신품종 작물육성에 있어 패러다임 변혁을 가져다 줄 핵심 기반기술이다. 본 연구에서는 CRISPR/Cas9를 이용하여 유전자편집기술을 기존에 알려진 방법보다 쉽고 정확하게 실험 할 수 있도록 sgRNA 디자인, 벡터구축, 형질전환체 육성 및 분석 등을 자세히 기술하였다. sgRNA는 http://www.rgenome.net/ 사이트에서 NGG 영역을 중심으로 하여 target-up: 5'-ggcaGNNNNNNNNNNNNNNNNNNNN-3'과 target-down: 5'-aaacNNNNNNNNNNNNNNNNNNNNC-3'의 올리고를 디자인하였다. 식물형질전환용 벡터는 pPZP-Cas9-RGEN을 기본으로 하였으며, sgRNA의 프로모터는 OsU3를 이용하여 pPZP::35S::Cas9::PinII-OsU3::sgRNA::Bar-Gen 순으로 구축하였다. 형질전환체의 육성은 단기형질전환 Agrobacterium 법을 사용하였으며 재분화 식물체를 얻는데48일 정도 소요되었다. 형질전환체 유무는 genomic PCR 분석으로 single copy 선발은 TaqMan PCR로 분석하였다. 정밀유전자편집 식물체는 T1 세대에서 T-DNA 삽입되지 않은 식물체를 Bar-strip에 의해 선발하였다. 선발된 식물체의 sgRNA 영역의 염기배열 조사에 의해 유전자 편집 식물체를 육성하였다. 따라서 본 연구에서 CRISPR/Cas9 system에 의한 정밀유전자편집 기술을 이용하여 보다 빠르고 쉽고 경제적으로 유전자가 편집된 개체를 확보할 수 있었다. 본 실험에서 확립된 system은 상업용 식물 계통육성에 이용 가능하여 육종적 가치가 매우 클 것으로 사료된다. The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

무용전공고등학생의 경쟁불안이 무용몰입 및 무용성취에 미치는 영향

정유진,민윤미,Jung, Yu-Jin,Min, Yun-Mi 한국디지털정책학회 2020 디지털융복합연구 Vol.18 No.9

이 연구는 무용전공고등학생의 경쟁불안이 무용몰입 및 무용성취에 미치는 영향을 규명하는데 목적을 두고 진행되었다. 연구대상은 2020년 현재 서울 및 수도권 소재 고등학교에서 무용을 전공하고 있는 학생이었으며, 조사도구는 설문지를 사용하여 온라인 설문을 실시하였다. 총 222명의 자료를 Windows용 SPSS 18.0을 통해 t-test, 일원변량분석, 상관분석, 다중회귀분석 및 위계적 회귀분석을 적용하여 자료 처리한 결과 다음과 같은 결론이 도출되었다. 첫째, 남학생이 여학생보다 상태자신감이 높고, 집안의 경제력이 중하위권이라고 인지하는 학생들의 인지불안이 높았다. 또한 상태자신감과 무용성취는 경제력이 상위권이라고 인지하는 집단이 중하위권보다 높은 것으로 나타났다. 둘째, 인지적 불안과 상태자신감은 무용몰입에 정적영향을 미치는 것으로 나타났다. 셋째, 인지적 불안과 상태자신감은 무용성취에 정적영향을 미치는 것으로 나타났다. 넷째, 무용몰입은 경쟁불안과 무용성취에 관계된 강력한 매개변인으로 나타났다. 이상의 결론을 종합해 볼 때, 무용전공 고등학생들의 경쟁불안은 무용에 몰입하는데 작용하며 무용 성취를 높이는 것으로 나타났으나, 이와 관련된 부작용이 있을 것으로 예견되는바 후속 연구에서는 이러한 심리적 변인들에 대한 추가 연구가 필요한 것으로 판단된다. The study was conducted with the aim of identifying the impact of competition anxiety on dance immersion and performance in high school students majoring in dance. As of 2020, online surveys were conducted on students majoring in dance at high schools in Seoul and the metropolitan area. A total of 222 people's data were processed by applying t-test, one-way variable analysis, correlation and multiple regression analysis through SPS 18.0 for Windows, resulting in the following conclusions. First, male students were more confident in their condition than female students, and students who recognized that their family's economic power was in the middle class were more aware. In addition, it was found that the group perceived that economic power was at the top of the list was higher than the median. Second, cognitive anxiety and self-confidence have been shown to have a static effect on immersion into dance. Third, cognitive anxiety and self-confidence have been shown to have a static effect on the achievement of uselessness. Fourth, immersion in dance appeared to be a powerful parameter related to competition anxiety and achievement of dance. To sum up the above conclusions, competition among high school students majoring in dance has been shown to increase their performance by immersing themselves in dance, but further studies of these psychological variables are expected to have side effects.

통합 의학 치료로 5년 생존 및 완전 관해에 도달한 췌장암 증례 보고 1례

정유진,김지수,박경덕,오유나,정범진,방선휘,Yu-jin Jung,Jisoo Kim,Kyung-Dug Park,Yoona Oh,Beom-Jin Jeong,Sunhwi Bang 대한한방내과학회 2023 大韓韓方內科學會誌 Vol.44 No.3

Objectives: This is a five-year survival and complete response (CR) report on pancreatic cancer treated with western medicine and Korean traditional medicine. Method: A 59-year-old woman diagnosed with pancreatic cancer visited ○○ Korean traditional medicine hospital after neoadjuvant chemotherapy and pylorus-preserving pancreaticoduodenectomy. She was treated with Korean traditional medicine, including acupuncture, abdominal moxibustion, wild ginseng pharmacopuncture, and herbal medicine, which was based on integrated medicine therapy (IMT), from March 2018 to September 2022. The tumor size was measured by scanning with computed tomography (CT), magnetic resonance imaging, and positron-emission tomography/CT. Adverse events were evaluated using laboratory conclusion and National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0. Result: During four years and three months of treatment, IMT maintained safety. The patient finally reached five-year survival without any recurrence or complication (CR) on October 26, 2022. Conclusion: We suggest that an integrative approach including Korean traditional medicine can be a meaningful treatment option for pancreatic cancer. Further studies should be performed to establish the proper treatment protocol of integrative medicine for pancreatic cancer.

ICT 원격제어 system 이용 식물진단, Phenomics 연구현황 및 전망

정유진,노일섭,김용권,김회택,강권규,Jung, Yu Jin,Nou, Ill Sup,Kim, Yong Kwon,Kim, Hoy Taek,Kang, Kwon Kyoo 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.1

Remote sensing는 각종 센서를 이용하여 지표면, 물, 요소 기술에 대해 비접촉, 비파괴적인 방법으로 필요한 정보를 얻어내는 기술이다. 이들 기술은 센서 등의 요소 기술 뿐만 아니라, 센서를 탑재하는 플랫폼과 정보 통신 기술(ICT) 등을 복합적으로 이용한다. 특히 농업 분야에서는 ICT를 중개로 기상이나 토양 등의 환경 정보와 작물 정보를 측정하여 수치화하고 클라우드 컴퓨팅에 의해 생산 단계뿐만 아니라 유통 및 소비 단계까지 관리하는 스마트 농업에 크게 기여한다. 식물을 측정하기 위해서는 비파괴 비접촉 bioimaging (remote imaging)을 포함한 식물기능 remote sensing 기술개발이 필요하다. 또한 식물 과학 분야에서도 유전자 세포 수준에서 개체 수준까지를 대상으로 한 bioimaging 연구가 활발히 진행되고 있다. 최근 들어 표현형 연구를 통해 환경과 유전자형의 관계를 구명하는 phenomics 연구가 활발히 진행되고 있다. 따라서 본 논문에서는 식물기능 원격탐사의 기술동향, 식물진단 및 식물환경응답해석과 식물 phenomics 연구현황에 대해 고찰하였다. Remote Sensing (RS) is a technique to obtain necessary information in a non-contact and non-destructive method by using various sensors on the surface, water or atmospheric phenomena. These techniques combine elements such as sensors, and platform and information communication technology (ICT) for mounting the sensor. ICT has contributed significantly to the success of smart agriculture through quantification and measurement of environmental factors and information such as weather, crop and soil management to distribution and consumption stage, as well as the production stage by the cloud computer. Remote sensing techniques, including non-destructive non-contact bioimaging (remote imaging) is required to measure the plant function. In addition, bioimaging study in plant science is performed at the gene, cellular and individual plant level. Recently, bioimaging technology is considered the latest phenomics that identifies the relationship between the genotype and environment for distinguishing phenotypes. In this review, trends in remote sensing in plants, plants diagnostics and response to environment and status of plants phonemics research were presented.

식물의 감수분열에서 상동 재조합 효소 특이 기능의 연구현황 및 전망

정유진,남기홍,김태성,이인혜,조용구,강권규,Jung, Yu Jin,Nam, Ki Hong,Kim, Tae Sung,Lee, In Hae,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.1

Meiosis is a specialized cell division, essential in most reproducing organisms to halve the number of chromosomes, thereby enabling the restoration of ploidy levels during fertilization. A key step in meiosis is homologous recombination, which promotes homologous pairing and generates crossovers (COs) to connect homologous chromosomes until their separation at anaphase I. These CO sites, seen cytologically as chiasmata, represent a reciprocal exchange of genetic information between two homologous non-sister chromatids. RAD51, the eukaryotic homolog of the bacterial RecA recombinase, plays a central role in homologous recombination (HR) in yeast and animals. Loss of RAD51 function causes lethality in the flowering plant, Arabidopsis thaliana, suggesting that RAD51 has a meiotic stage-specific function that is different from homologous pairing activity.

신식물육종기술의 현황과 사회적 수용을 위한 노력

정유진,김종미,박수철,조용구,강권규,Jung, Yu Jin,Kim, Jong Mi,Park, Soo-Chul,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.4

Although new plant breeding technologies facilitate efficient plant breeding without introducing a transgene, they are creating indistinct boundaries in the regulation of genetically modified organisms (GMOs). The rapid advancement in plant breeding by genome-editing requires the establishment of a new global policy for the new biotechnology, while filling the gap between process-based and product-based GMO in terms of regulations. In this study recent developments in producing major crops using new plant breeding technologies were reviewed, and a regulatory model that takes into account the various methodologies to achieve genetic modifications as well as the resulting types of mutation were proposed. Moreover, the communication process were discussed in order to understand consumers' current situation and problems of new plant breeding technology, establish social acceptance well, and understand consumers' disputes such as GMO crops.

NGS 기술 활용 돌연변이체 해석 및 연구현황

정유진,류호진,조용구,강권규,Jung, Yu Jin,Ryu, Ho Jin,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.4

NGS 기술은 전체 게놈 시퀀싱 및 reference 게놈에 alignment에 의해 돌연변이 표현형에 관련된 돌연변이 식별에 이용한다. 그러나 품종 및 계통들을 resequence 하였을 경우 기존의 reference 게놈에 구조적 변이가 보이며, reference와 맞지 않는 게놈지역에서 돌연변이들은 단순한 alignment로 찾을 수 없다. 본 리뷰에서는 NGS 기술을 이용하여 돌연변이체로부터 변이 관련 유전자를 식별하는 MutMap, MutMap-Gap 및 MutMap+ 방법을 기술하였고 지금까지의 연구현황에 대해 기술하였다. 아울러 이들 방법은 nucleotide-binding site-leucine rich repeat (NBS-LRR) 그룹들의 병 저항성 유전자와 같이 구조적 변이를 가진 유전자를 분리하는 등 유용성에 대해 고찰하였다. Next-generation sequencing allows the identification of mutations responsible for mutant phenotypes by whole-genome resequencing and alignment to a reference genome. However, when the resequenced cultivar/line displays significant structural variation from the reference genome, mutations in the genome regions absent in the reference cannot be identified by simple alignment. In this review, we report the current status and prospects in identification of genes in mutant phenotypes, by using the methods MutMap, MutMap-Gap, and MutMap+. These methods delineate a candidate region harboring a mutation of interest, followed by de novo assembly, alignment, and identification of the mutation within genome gaps. These methods are likely to prove useful for cloning genes that exhibit significant structural variations, such as disease resistance genes of the nucleotide-binding site-leucine rich repeat (NBS-LRR) class.