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정규식(Kyu Shik Jeong) 한국예방수의학회 2000 예방수의학회지 Vol.24 No.3
Cytochrome P4501A1 (CYP1A1)의 유전자 분포에 대한 광범위한 연구에도 불구하고, 현재까지 간장외 조직에서의 유전자 발현양상에 대한 연구는 미미한 실정이다. 본 연구에서는 면역조직화학적 방법, reverse transcription polymerase chain reaction (RT-PCR), Southern analysis, in situ RT-PCR 방법으로 간, 이자, 신장, 폐 및 뇌에서 CYP1A1유전자 발현양상을 관찰하였다. CYP1A1 mRNA 및 단백질은 2,3,7,8-tetrachlorodibenzo-p-dioxin 혹은 YH439 투여에 의해 유도되었는데, 간장에서는 주로 간문맥주변에 나타났으며, 신장에서는 세뇨관 상피세포에서, 폐에서는 기관지 상피세포에서, 그리고 이자에서는 α-cells에서 동정이 되었으나, in-situ RT-PCR 및 면역조직염색상에서는 관찰되지 않았다. 이와 같이 CYP1A1 mRNA 및 단백질이 세포 특이적으로 발현된다는 사실은 환경오염물질과 같은 유해물질에 노출되었을 경우 방어기전에 중요한 기능을 할 것으로 사료된다. Despite extensive research on the induction mechanism of the cytochrome P4501Al (CYPlAl) gene, its regional expression in extra-hepatic tissues is not well characterized. Here we determined the localization of CYPIAl expression using immunohistochemistry, reverse transcription and polymerase chain reaction (RT-PCR), Southern analysis and in-situ RT-PCR. The levels of CYP1Al mRNA and protein, undetectable in untreated rat tissues, were significantly elevated by treatment with either 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or a synthetic thiazolium compound, YH439. Elevated levels of CYP1A1 mRNA transcript and immunoreactive CYP1A1 protein were mainly observed in the periportal region of the liver, the cortical tubular epithelium of the kidney, bronchiolar epithelial cells of the lung and peripheral cells of the pancreatic islets, most likely a-cells. In contrast, neither CYP1A1 mRNA nor its protein was detected in brain by in situ RT-PCR and immunohistochemistry even after treatment with YH439 or TCDD, although an increased level of CYP1A1 mRNA transcript in brain was recognized by RT-PCR followed by Southern analysis. The selective regional distribution of CYP1A1 mRNA and protein may play a role in the preferential toxicity of certain extra-hepatic cells exposed to potentially toxic agents including environmental carcinogens.
Ethanol metabolism stops gut glutamine uptake
정규식(Kyu Shik Jeong) 한국예방수의학회 2000 예방수의학회지 Vol.24 No.3
일반적으로 장관세포 및 결장세포들은 주요대사 에너지원으로 L-glutamine을 이용하지만, 특정 short chain fatty acid 및 acetate 등은 장관상피세포에서 에너지 기질로 사용이 되어진다. 본 연구에서는 알코올의 대사산물인 아세테이트와 장관흡수 아미노산과의 상호경쟁 이용도를 탐색하고자, 웅성 랫드의 (250~300g) 간문맥, 간정맥, 경동맥에 cannulae을 설치하여 장관을 경유한 아미노산과 아세테이트의 동맥과 간문맥사이의 흡수도를 조사하였다. L-glutamine의 장흡수율은 정상군에서는 0.13±0.03 μmol/ml plasma 이지만, 알코올을 투여한 경우 아세테이트로 전환 되는 과정에서 L-glutamine 은 0.12±0.03 μmol/ml plasma 순수 배출되는 반면, acetate는 0.22 μmol/ml plasma에서 알코올 투여후 0.52 μmol/ml plasma로 전환되었다. 이상의 결과로 보아 알코올 대사과정에서의 발생되는 acetate는 장관에서 L-glutamine의 흡수를 방해하여, 에너지원으로 L-glutamine과 경쟁적인 관계를 유지하는 것을 관찰하였다. It is now generally accepted that enterocytes and colonocytes preferentially use L-glutamine as their primary metabolic fuel. However, short chain fatty acids, such as acetate, are also substrates for intestinal epithelial cells. The aim of this study was to examine the possible competition between acetate, the metabolic product of ethanol upon the gut uptake of amino acids, particularly the gut uptake of L-glutamine. Accordingly, we administered ethanol by gavage to ad lib fed 250~300g male rats in which chronic cannulae were implanted in the portal vein and the external carotid artery and measured the artereo-venous differences of amino acids across the splanchnic bed. The gut of control rats took up mainly L-glutamine at rates of about 0.13±0.03 μmol/ml plasma. However, during the conversion of ethanol to acetate, gut became a net exporter of L-glutamine at a rate of about 0.12±0.03 μmol/ml plasma while at the same time reversing its normal output of acetate from 0.22 μmol/ml plasma to a net uptake of acetate at a rate of 0.52 μmol/ml plasma. The production of acetate during ethanol metabolism changed gut from a L-glutamine importing to an exporting organ while changing acetate flux in the opposite direction.
폐렴우와 Klebsiella pneumoniae 실험적 감염 랫드의 기관 및 기관지 점막층에서 Globule leukocyte의 출현양상
박상준,이차수,정규식,Park, Sang-joon,Lee, Cha-soo,Jeong, Kyu-shik 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.4
The study was aimed at identifying the globule leukocytes (GL) of tracheal mucosa layer of Korean native cattle showing symptom of pneumonia which have died as enterotoxemia and normal Korean native cattle in Kyungpook local area. In another set of experiment, Isolated Klebsiella pneumoniae from suddenly died cattle specimens was subjected to rat for a determining globule leukocyte appearance by using histochemical and immunohistochemical method. In histochemical study, globule leukocytes generally was existed in all the case of postmortem of Korean native cattle and 3 heads of slaughtered cattle which showing symptoms of pneumonia and it showed significant increase in tracheal mucosa of rats experimentally infected with Klebsiella pneumoniae. These increased number of globule leukocytes was moderately remained on early infection stage and gradually decreased in timedependent manner after infection. The granule patterns were also determined as an acidmucopolysaccharide. In immunohistochemical study, serotonin intensity in the treacheal mucosaepithelial cells of rat experimentally infected observed a strong immunoreactivity during early infection and gradually decreased in dependent of infection stage while no IgE immunoreactivity observed. These data show that globule leukocytes were increased in a pneumonia, therefore it was considered as a valuable cell that was associated with early stage of inflammatory response.