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이호원(Howon Lee),이동규(Dongkyu Lee),윤희상(Heesang Yoon),송인회(Inhoi Song),윤경수(Kyungsu Yun),기석철(Seok-Cheol Kee) 한국자동차공학회 2017 한국자동차공학회 학술대회 및 전시회 Vol.2017 No.11
미래형 자동차 기술은 운전자의 편의성을 증진시키고 환경규제에 유연한 친환경 자율주행 전기자동차가 될 것이다. 차량의 자율주행을 위해서는 Lidar, GPS, Camera 등의 센서 정보를 이용하여 주변환경 인지 및 주행 경로를 생성, 자동차가 목표로 하는 지점까지 안전하게 이동할 수 있어야 한다. 전세계 각국 및 많은 기업들이 이러한 자율주행차의 기술을 선점하기 위해 많은 노력을 기울이고 있으며, 그 연구결과들이 상용차에 서서히 적용되어 출시되고 있다. 본 논문에서는 초소형 전기자동차를 이용하여 자율주행이 가능한 하드웨어 플랫폼을 개발하였고, 이 차량을 제어하기 위한 VCU 또한 개발하였다. 그 결과 개발된 초소형 자율주행 전기차는 VCU의 CAN 인터페이스를 개방하여, 이를 통한 지능형 자동차 개발 방법을 공유하고, 연구 개발의 진입 장벽이 없는 공개형 참조 플랫폼으로서 제안하고자 한다.
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개 바베시아병에 관한 연구 I. Babesia gibsoni의 시험관내 분리와 항원성상에 관한 연구
이호권,서명득,Lee, Ho-kweon,Suh, Myung-deuk 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3
The present study was conducted to isolate Babesia gibsoni by culture method of the microaerophilous stationary phase(MASP) and analyse the antigenic properties of the parasite by SDS-PAGE and immunoblot. The results obtained were summarized as follows. The protozoan parasite Babesia gibsoni multiplied in canine erythrocytes in RPMI 1640 medium(pH7.0) containing 20 40% normal canine serum under the MASP condition of 5% CO2 and 95% air at $37^{\circ}C$ incubator. The levels of parasitaemia in the erythrocytes were shown more higher by exchanging the medium at 24 hours interval. Under the above condition of MASP, the percentage of parasitized erythrocytes(PPE) after incubation for 8 days increased about 14 times more than that in the initiation of the 1% infected canine erythrocyte culture. The parasites were purely isolated from the MASP culture of red blood cells collected from dogs infected with Babesia gibsoni naturally or artificially. Among the total of 36 canine(Pit-bullterier) blood samples the parasites were isolated from 17 cases(47.2%) in the MASP culture while the parasites were detected from 20 cases(56%) and 12 cases(33.3%), respectively, by indirect fluorescent antibody(IFA) test and direct light microscopy(DLM). On the other hand, Babesia gibsoni was isolated by MASP culture from 15 cases(75%) and 11 cases(92%) of positive cases of IFA and DLM, respectively. In the analysis of the erythrocytic merozoite(AEOM) antigen derived from infected dog approximately 11 antigenic bands in molecular weight of 130, 120, 97.4, 92, 80, 52, 50, 42, 36, 30 and 29 KDa were observed on SDS-PAGE. Antigenic bands in the endoerythrocytic merozoite(CEOM) antigen derived from infected erythrocyte (sediment) in MASP culture were much similar to those of AEOM bands. In the exoerythrocytic merozoite(CEEM) antigen derived from supernatant of the infected erythrocyte culture approximately 20 antigenic bands were observed and the molecular weight of the major bands among these were 140, 120, 114, 105, 96, 93, 92, 80, 60, 52, 50, 38, 36, 30, 24, 18.5 and 16 KDa. In the protein patterns of AEOM and CEOM antigen by immunoblot 15 bands were observed and these patterns were much similar between each other. The molecular weight of the major bands in the both antigens were 130, 120, 80, 60, 52, 50, 42, 30, 29, 18.5 and 16 KDa. Approximately 21 bands were observed in CEEM antigen and the molecular weight of the major bands were 140, 120, 96, 92, 85, 80, 76, 60, 52, 50, 37, 30, 24, 16 and 15 KDa. The specific antigenic bands in the artificially infected dogs were firstly observed at 3 weeks afrer inoculation of infected blood and these antigenic bands were maintained up to 18 months after inoculation. In the immunoblot of the sera of the splenectomized dogs the specific antigenic bands with the molecular weight of 93 KDa and 52 KDa, respectively, were observed weakly comparing to those of non-splenectomized dog. In immunoblot of the sera collected from the naturally infected dogs the antigenic bands were observed as same as those of artificially infected dogs while antigenic band of 29 KDa in some individual dog showed strongly. In comparison of immunoblot of the sera collected from dogs non-treated and treated with diminazene aceturate(7mg/kg, IM) after artificial infection no differences of antigenic bands were observed. In analysis of antigenic bands by digoxigenin glycan/protein double labeling, antigenic bands in the molecular weight of 106, 60 58, 36, 30 and 29 KDa were determined as glycoproteins.
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터널 화재 시 열부력을 고려한 제연용 제트팬 용량산정에 관한 기초 연구
이호형,최판규,조종복,이승철,이창우,Lee, Ho-Hyung,Choi, Pan-Gyu,Jo, Jong-Bok,Lee, Seung-Chul,Lee, Chang-Woo 한국터널지하공간학회 2018 한국터널지하공간학회논문집 Vol.20 No.2
최근 '도로터널 방재시설 설치 및 관리지침'의 개정으로 인해 터널 내 제연용 제트팬의 용량 산정 시에는 열부력을 고려하도록 규정하고 있다. 그러나 열부력을 고려하는 세부방법론에 대한 규정이 없어, 이에 대한 세부적인 추가 연구가 필요한 실정이다. 본 연구에서는 터널 내 화재 시 열부력을 고려하기 위하여 3차원 수치해석 시뮬레이션을 수행하여 터널 내열부력을 계산하고, 화재차량의 진행방향에 따라 열부력과 차량항력의 관계, 즉, 화재차량의 위치에 따른 터널 내 제연용 제트팬 용량 산정방법에 대하여 검토를 수행하였다. 분석결과에 따르면, 하향경사 터널의 경우에는 열부력이 터널 내 저항력으로 작용하며, 터널 내 화재연기를 제연하기 위해 필요한 제연팬의 승압력은 단순히 터널 입구부의 열부력 값과 출구부의 차량항력값에 의해 단순히 결정되지 않으며, 터널 내 화재차량의 위치에 따라 종합적인 검토가 필요한 것으로 분석되었다. As a result of the recent revision of the 'Guideline for Installation and Management of Fire Prevention Facility in Road Tunnels', the thermal buoyancy has to be taken into account when calculating the capacity of jet fans for smoke control in tunnel fires. However, there is no detailed methodologies for considering thermal buoyancy, so further study is needed. In this study, the thermal buoyancy in the tunnel is calculated by 3-D numerical simulation to consider the thermal buoyancy in case of fire in tunnels, and the relationship between heat buoyancy and vehicle drag, And the method of calculating the capacity of the jet fan for smoke control in tunnels. According to the analysis results, heat buoyancy acts as a resistance force in the case of a down-slope tunnel, and the pressure rise of jet fan for smoke control is not simply determined by the value of heat buoyancy at the entrance of the tunnel and the value of the vehicle drag at the exit. And it is analyzed that it is necessary to carry out a comprehensive review according to the location of the fire vehicle in tunnels.
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외인성 insulin-like growth factor-I이 육계의 성장에 미치는 영향
이호일,이문준,이대열,김영안,강창원,전승기,Lee, Ho-il,Lee, Moon-joon,Lee, Dae-yeoul,Kim, Young-ann,Kang, Chang-won,Chon, Seung-ki 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.3
Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{\mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days($1,176.50{\pm}99.79g$) and 35 days($1,252.84{\pm}125.21g$) of age in treatment groups, compared with control group($1,011.88{\pm}40.22g,\;1,111.32{\pm}153.67g$). The liver and kidney weights on 35 days$(35.24{\pm}5.18g,\;11.05{\pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group($30.95{\pm}4.04g,\;10.01{\pm}1.60g$) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{\pm}1.69ng/ml$, $3.75{\pm}0.62g/dl$ respectively compared with control group $45.70{\pm}1.64ng/ml$, $2.32{\pm}0.53g/dl$. The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum.
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이호,Lee, Ho 한국미생물학회 1991 微生物과 産業 Vol.17 No.3
최근 유산균주의 개량에 대한 연구는 세가지 중요한 영역에 그 촛점을 맞추고 있다. 첫째 앞으로 산업적으로 중요한 유전자의 성격규명과 cloning, 둘째 유산균에 적합한 유전자 전달계(gene transfer system)의 개발, 셋째 cloning vector의 개발이다(Froseth와 McKay,1991). 이 논문에서는 발효제품에 관여하는 유산균들의 유전학적 연구 및 새로운 균주개량법에 대하여 최근 발표된 자료를 토대로 기술하려 한다.
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이호 회계사의 세금 칼럼-서류를 챙기면 양도소득세 줄일 수 있다.
이호,Lee, Ho 한국주택협회 2007 주택과 사람들 Vol.206 No.-
공익사업용으로 수용되는 특별한 경우<기준시가 적용>를 제외하고는 올해부터 양도소득세를 계산하는 경우에 실지거래가액을 적용한다. 실지거래가액으로 앵도소득세를 계산하는 경우에는 부동산을 취득하는 시점부터 관련 증빙자료를 꼼꼼히 챙기고 보관해야 세금을 추가 부담하는 일이 없다.
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