1.5 T 자기공명영상기기에서 수소 자기공명분광법을 이용한 모델용액 내 포도당의 정량분석

이경희,이정희,조순구,김용성,김형진,서창해,Lee, Kyung-Hee,Lee, Jung-Hee,Cho, Soon-Gu,Kim, Yong-Seong,Kim, Hyung-Jin,Suh, Chang-Hae 대한영상의학회 2002 대한영상의학회지 Vol.46 No.1

목적: 1.5T 생체용 자기공명영상기기를 이용한 수소자기공명분광법으로 용액 내 물질의 정량분석에 대한 가능성을 알아보고자 하였다. 대상과 방법: 0.01%에서 50%까지의 여러 농도를 갖는 포도당+증류수 혼합액과 0.01%에서 20%까지의 여러 농도를 갖는 포도당+증류수+에탄올 혼합액의 모델용액을 만들어 생체용 자기공명영상기기와 시험관 nuclear magnetic resonance(NMR)분광기에서 각각 수소자기공명분광법을 시행하여 스펙트럼을 얻었다.스펙트럼 상에서 포도당 농도에 따른 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비의 변화를 구하였고, 통계처리는 상관분석과 단순선형회귀분석을 시행하였고 회귀식을 산출 하였다.또한 생체용 자기공명영상기기를 이용하여 얻은 결과가 객관적인지 알아보기 위해 시험관 NMR 분광기에서 얻은 결과와의 상관관계를 분석하였다. 결과: 생체용 자기공명영상기기를 이용한 스펙트럼상 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비는 포도당 농도변화에 대하에 일정하게 증가하는 통계적으로 의미 있는 변화를 보였고 그 결과에 따라 회귀식을 구할 수 있었다. 생체용 자기공명영상기기와 시험관 NMR 분광기를 이용하여 얻은 모든 결과에 대한 상관분석에서 통계적으로 의미 있는 상관관계를 보여 생체용 자기공명영상기기에서 얻은 수소자기공명분광 소견을 객관화 할 수 있었다. 따라서 생체용 자기공명영상기기를 이용하여 얻은 스펙트럼에서 용액 내 물질의 피크면적비를 구함으로써 농도를 정량할 수 있는 결과를 얻을 수 있었다. 결론: 생체용 자기공명영상기기를 이용하여 얻은 수소자기공명분광 스펙트럼에서도 시험관 NMR분광기로 얻은 스펙트럼과 마찬가지로 단순 포도당 용액, 포도당에 에탄올을 혼합한 용액에서 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비를 측정함으로써 당의 정량분석이 가능하였다. 본 연구의 결과는 생체수소자기공명분광법이 환자의 체액에 대한 정량적 분석에 이용될 수 있다는 가능성을 시사한다고 생각한다. Purpose: To evaluate the feasibility of proton magnetic resonance spectroscopy ($^1H-MRS$) using a 1.5T magnetic resonance (MR) imager for quantification of the contents of model solutions. Materials and Methods: We prepared model solutions of dextrose+water and dextrose+water+ethanol at dextrose concentrations of 0.01% to 50% and 0.01% to 20%, respectively. Using these solutions and a 1.5T MR imager together with a high-resolution nuclear magnetic resonance (NMR) spectroscope, we calculated the ratios of dextrose to water peak, (dextrose+ethanol) to water peak, and (dextrose+ethanol) to ethanol peak, as seen on MR and NMR spectra, analysing the relationships between dextrose concentration and the ratios of peaks, and between the ratios of the peaks seen on MR spectra and those seen on NMR spectra. Results: Changes in the ratios between dextrose concentration and dextrose to water peak, (dextrose+ethanol) to water peak and (dextrose+ethanol) to ethanol peak, as seen on MR spectra, were statistically significant, and there was good linear regression. There was also close correlation between the ratios of the observed on MR and NMR spectra. The results depict the quantification of dextrose concentration according to the ratios of spectral peaks obtained by proton MRS at 1.5T. Conclusion: Using proton MRS at 1.5T, and on the basis of the ratios of spectcal peaks, it was possible to quantify the concentration of dextrose in model solutions of dextrose+water and dextrose+water+ethanol. The results of this study suggest that for quantifying the contents of biofluids, the use of low-tesla $^1H-MRS$ is feasible.

LPS로 유발된 대식세포의 염증반응에 대한 청상보하탕(淸上補下湯)의 효과

이경희,김홍렬,정희재,이형구,Lee, Kyung-Hee,Kim, Hong-yeoul,Jung, Hee-Jae,Lee, Hyung-Koo 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti-histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT. Results : Results shows that the CSBHT did not affected cell survival within $10^{1}$ ng/ml to $10^{5}$ ng/ml. Especially, $10^{5}$ ng/ml CSBHT treated cells show 70% deduction of $TNF-{\alpha}$ gene expression against that of LPS treated group. Furthermore, $IL-1{\beta}$, IL-6, IL-8, IL-10 and $TNF-{\alpha}$ levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 ($TNF-{\alpha}$ inhibitor). At protein level, the expression of $TNF-{\alpha}$ showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ${\pm}$ 87 pg/ml) than in the LPS-treated group (703 ${\pm}$ 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ${\pm}$ 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.

ROBOTC기반 LEGO MINDSTORMS NXT 로봇을 이용한 교육과정 개발 및 교육효과 분석

이경희,Lee, Kyung-Hee 한국정보처리학회 2011 정보처리학회논문지 A Vol.18 No.5

본 논문에서는 대학생 대상의 ROBOTC 기반 레고 마인드스톰 NXT 로봇을 이용한 교육과정 개발 내용을 보이고, 이 과정에 대한 교육효과를 분석하였다. 교육과정은 로봇의 기본 이해와 실습, 응용로봇 실습, 창의로봇 설계 및 구현으로 구성하였다. 2009년부터 3년간 교육을 진행하는 동안, 6개 분반 총 94명의 수강생을 대상으로 설문조사를 실시하여 교육효과를 분석하였다. 분석 결과를 통해 레고 마인드스톰 NXT 로봇을 이용한 교육과정이 학습동기와 학습의욕을 불러 일으켰고, 학생들의 교과목 교육목표 및 학습성과 달성도가 우수하였음을 보인다. 또한 학생들의 수업 및 과제물 수행에 대한 참여도가 매우 높았으며, 문제해결능력과 창의력 향상에 도움을 주었고, 조별 프로젝트 수행으로 협동심도 향상되었다. 마지막으로 ROBOTC기반 프로그래밍에 의한 로봇 제어 실습으로 C언어 프로그래밍 능력 향상에도 도움을 준 것으로 나타났다. In this paper, we show how a curriculum using LEGO MINDSTORMS NXT robot based ROBOTC for undergraduate students has been developed, and we analyze the educational effect of the curriculum. The curriculum is composed of basic knowledge learning, practice with basic robots, practice with advanced robots, and creative design and implementation of robots. During the three year period since 2009, educational achievement has been analyzed by surveys for 6 classes, 94 students. According to the analysis, the curriculum has highly motivated the students and made them to achieve effectively our educational and academic goals. Also, we observe that the curriculum helped the students to improve their creativity and the problem solving skill, and that the students were autonomously and deeply involved in the homework and the term projects, which made them be very cooperative. Finally, the intensive practice with ROBOTC programming is shown to help students to improve their programming ability of C language.

매선요법을 가미한 복합한방치료를 시행한 자발성 척수 지주막하 출혈 후유증환자 치험례

이경희,노주환,윤현민,장경전,안창범,김철홍,Lee, Kyoung-Hee,Lo, Ju-Hwan,Youn, Hyoun-Min,Jang, Kyung-Jeon,Ahn, Chang-Beohm,Kim, Cheol-Hong 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.2

Objective Spinal SAH is an unusual disease that occasionally occurs spinal cord injury. This report intended to estimate the effect that taken by using oriental treatment on the patient with the sequelae of spinal SAH. Methods We have observed this case of patient treated by Dong's acupuncture therapy, pharmacopuncture therapy, Mae-sun therapy and herbal medication, etc. Results The patient showed improvements in pain, power and sensory function. Conclusion Oriental treatments such as Dong's acupuncture therapy, pharmacopuncture therapy, Mae-sun therapy and herbal medication can be effective for the sequelae of spinal SAH.

한증막(汗蒸幕)의 시설 및 이용 실태 조사

이경희,박흥기,Lee, Kyung-Hee,Park, Hung-Ki 대한물리치료과학회 1994 대한물리치료과학회지 Vol.1 No.1

The Han Zeong Mag was the typical hydro-therapy of Korea. It looks like hot air bath of western physical therapy. It developed out of the CHO-SEON, King SE JONG(1422). It is warmed fire of pine wood in the room. At first, it runs a temperature about a few hundred and drop to $80^{\circ}C$ through 12 hours then the room air is exceeding dry. After 12h. reheated in the room. Women makes use of that place with bare body. Because for use salt rub of skin. They have low back pain, pain of muscle, muscle spasm, swelling of arthritis, stiffness of joint and it takes effect on the cosmetic of skin. The treatment effect was excellent to pain(28%), next arthritis(25%). But they have not order for treatment of a doctor. It's dangers of heat stroke and heart disease. Imtortant, must educated method of it's use and danger when it's bad use.

올란자핀의 체중증가 부작용 발생율 및 체중변화량

이경희,스리니바산샨무감,렌가라잔바스카란,산토쉬쿠마르나가야스리라만,용철순,최한곤,우종수,유봉규,Lee, Kyung-Hee,Shanmugam, Srinivasan,Baskaran, Rengarajan,Nagayya-Sriraman, Santhoshkumar,Yong, Chul-Soon,Choi, Han-Gon,Woo, Jong-Soo,Yoo, Bong-Kyu 대한약학회 2008 약학회지 Vol.52 No.4

Olanzapine, an atypical antipsychotic, has been widely used for the treatment of schizophrenia and bipolar disease. Although olanzapine is less associated with extrapyramidal symptoms and neuroleptic malignant syndrome compared to existing typical antipsychotics, the use of this drug has a problematic side effect of weight gain, which may cause metabolic syndrome such as type 2 diabetes. However, there are few hospitals practicing body weight monitoring of the patients on olanzapine or other atypical antipsychotics. The goal of this study was to identify the incidence and severity of weight gain associated with the use of the drug in Korea. We performed body weight monitoring of the patients who were on the drug in a hospital setting. Mean of the weight gain (as of one-month-transformation) was 4.33 and 3.39 kg for the male and female patients, respectively. The incidence in the young patients was higher than that observed in the old patients, and the severity was the highest in patients in their thirties followed by twenties or younger. This result suggests that the pattern of the weight gain associated with the use of olanzapine in Korea is similar to the reports performed and documented in US and European countries. Therefore, it appears that healthcare professionals in Korea should also watch on the weight gain issue in patients who are on olanzapine or other atypical antipsychotics.

락침(落枕) 환자에 대한 Sweet Bee Venom과 Bee Venom의 치료효과 및 Allergy 반응 비교 연구

이경희,윤현민,고우신,송춘호,장경전,안창범,김철홍,Lee, Kyoung-Hee,Youn, Hyoun-Min,Ko, Woo-Shin,Song, Choon-Ho,Jang, Kyung-Jeon,Ahn, Chang-Beohm,Kim, Cheol-Hong 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.4

Objective The purpose of this study is to investigate the difference of treatment effects and allergic responses to stiff neck between Bee Venom Pharmacopuncture and Sweet Bee Venom Pharmacopuncture. Methods Forty one patients who felt stiff neck were randomly divided into two groups, a Bee Venom Pharmacopuncture group(group I) and a Sweet Bee Venom Pharmacopuncture group(group II). Evaluations of the treatment effects were made before and after a treatment using Visual Analog Scale(VAS), Neck Disability Index(NDI), Clinical Evaluation Grade(CEG). The comparison of allergic responses was measured with VAS. The obtained data were analyzed and compared with SPSS. Results The group I and group II showed significant improvement(p<0.05) according to the VAS, NDI, CEG. And the differences between the two groups were insignificant according to VAS, NDI, CEG. But allergic responses such as localized edema, localized itching were significantly lower in group II than group I. Conclusions It seems that there are no big different treatment effects between the two groups. Sweet Bee Venom Pharmacopuncture appears to be more effective measurement against allergic reactions than the Bee Venom Pharmacopuncture. Further studies are needed for the comparison of Bee Venom Pharmacopuncture and Sweet Bee Venom Pharmacopuncture.

자연분만을 위한 산전·산후운동에 관한 고찰

이경희,Lee, Kyung-Hee 대한물리치료사협회 1988 대한물리치료사협회지 Vol.9 No.2

Casein Kinase Ⅱ에 의한 송아지 흉선 DNA Topoisomerase Ⅱ 의 활성조절

이경희,배영석 ( Kyung Hee Lee,Young Seuk Bae ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2

In order to study the modulation mechanism of topoisomerase II (topo II) activity, casein kinase II was purified from bovine liver using DEAF-cellulose, phosphocellulose, hydroxyapatite, and heparin-agarose column chromatography. Analysis of the purified enzyme by sodium dodesyl sulfate-polyacrylamide gel electrophoresis revealed three bands of molecular mass of 44, 42, and 26 kDa. Casein kinase II phosphorylated calf thymus topo II, and the phosphorylation stimulated topo II activity by 2 fold over that of unmodified enzyme. The treatment of calf thymus topo II with alkaline phosphatase abolished the enzyme activity almost completely. These results suggest that some of topo II enzymes exist as phosphoproteins in calf thymus and calf thymus topo II activity can be regulated by casein kinase II.

Rhodosporidium toruloides 로 부터의 RNA 연결효소의 몇 가지 성질들

이경희,정연희,남정이,박인원 ( Kyung Hee Lee,Yean Hee Joung,Jeong E . Nam Shin,In Won Park ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.2

A novel RNA ligase activity has been detected in a cell-free extract from Rhodosporidium toruloides. The enzyme system was found to successively join 2`-, 3`-, or cyclic 2` : 3`-nucleotide to the 5`-phosphate of synthetic U_(16)G, leading to the synthesis of N_nU_(16)G(n=1,2,3...). The ligation of the nucleotides with synthetic oligonucleotide was found to proceed by forming 2`-phosphomonoester, 3`, 5`-phosphodiester intermediate linkage. The RNA ligase requires ATP and Mg^(2+) for its maximum activity. Interestingly, treatment of the cell- free extract with micrococcal nuclease but not with deoxyribonuclease abolishes the enzyme activity, indicating that the RNA ligase requires an RNA component(s) for its ligation activity.