재조합 인체 인터루킨 - 2 1 . 분리정제 및 생화학적 특성

윤혜영,최혜림,이명규,김승호,나도선,이선복,한문희,함경수 ( Hye - Young Yun,Hye - Lim Choi,Myeong Kyu Lee,Seung Ho Kim,Doe Sun Na,Sun Bok Lee,Moon H . Han,Kyung - Soo Hahm ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.2

Recombinant human interleukin-2 (rH IL-2, Ser^(125)-rH IL-2) was purified to apparent homogeneity from E. coli in high yield and characterized its biochemical properties for the establishment of preclinical screening system and therapeutic applications. The purification was carried out by methods involving isolation of inclusion body, urea extraction, solubilization and gel filtration chromatography. The renaturation of the product was achieved by extensive dialysis against the storage buffer. The purity was confirmed by SDS-PAGE and HPLC. Amino terminal amino acid analysis and partial amino acid sequence analysis showed that the primary structure of the recombinant protein (rH IL-2) was found to be identical with natural IL-2. The purity and the conformation of the rH IL-2 were also confirmed by obtaining crystals of the recombinant protein.

B 형 간염바이러스 표면항원 중 Pre - S2 부위의 항원구조 및 중합 알부민 수용체의 특성

윤혜영,박필련,김승목,함경수 ( Hye Young Yun,Pil Yon Park,Seung Moak Kim,Kyung Soo Hahm ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.2

Pre-S2 region (amino acids 120-174) of hepatitis B virus surface antigen contains immunodominant and disulfide bond-independent epitopes. Using pre-S2 peptides (recombinant peptides 121-174/adw2, 134-170/adw2 and synthetic peptide 120-145/ayw), epitope and polyalbumin receptor region on pre-S2 were studied. Polyalbumin receptor assay and enzyme immunoassay provided evidences that the region of at least 12 residues (134-145) of pre-S2 was shared by epitope for an anti-pre-S2 monoclonal antibody and polyalbumin receptor. This region was shown to be hydrophilic and a possible β-sheet.

Immunoaffinity Purification and Receptor Assay for Polymerized Human Serum Albumin of Pre-S2 Peptide on Hepatitis B Virus

윤혜영,한문희,함경수,Yun, Hye-Young,Han, Moon-H.,Hahm, Kyung-Soo 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

대장균내에서 과량 발현된 재조합 프리-S2 펩티드를 면역친화력 크로마토그래피에 의해 분리 정제하였으며, 이 펩티드에 대한 중합 인혈청 알부민(pHSA)의 결합을 연구하였다. 프리-S2 부위의 pHSA수용체 활성을 검출하는 방법을 프리-S2-$\beta$ 갈락토시다제 융합단백질을 이용하여 개발하였으며, 민감도는 10-100 p mol의 프리-S2에 해당되었다. 또한 이 방법은 프리-S2 농도 결정 뿐 아니라 pHSA수용체 활성을 중화시킬 수 있는 인자들을 측정하는데 이용될 수 있다. Recombinant pre-S2 peptide was purified to apparent homogeneity from E. coli by immunoaffinity chromatography using monoclonal antibody and studied for its binding characteristics with monoclonal antibody and pHSA (polymerized human serum albumin). A simple and sensitive assay method for detecting the activity of pHSA receptor on pre-S2 peptide of hepatitis B virus surface antigen (HBsAg) has been developed using pre-S2-$\beta$-galactosidase fusion protein. The assay can be used for determining pre-S2 or factors neutralizing pHSA receptor activity. The sensitivity of the assay is about 10 to 100 pmol of pre-S2.

Antigenic Structure and Polyalbumin Receptor Characteristics of Pre-S2 Region of Hepatitis B Virus Surface Antigen

윤혜영,박필련,김승목,함경수,Yun, Hye-Young,Park, Pil-Yon,Kim, Seung-Moak,Hahm, Kyung-Soo 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

B형 간염바이러스(HBV) 표면항원 단백질(HBsAg) 중 55개의 아미노산으로 구성된 pre-S2 부위를 재조합 대장균으로부터 순수 분리하였다. 이 부위의 펩티드 절편들(121-174/adw2, 134-170/adw2, 120-145/ayw)은 anti-pre-S2(adw2) 단일클론항체 및 중합 인혈청 알부민에 결합하는 결과를 보였으며 이는 세 펩티드의 공통서열인 134-145 부위에 항원 결정부위 및 중합 인혈청 알부민 수용체가 존재하는 것을 암시한다. 134-145 부위는 HBV의 여러 serotype들에서 보존된 서열을 포함하며, 2차구조로 예측 결과 ${\beta}$-sheet 형성의 높은 가능성을 보인다. 또한 anti-pre-S2 단일클론항체가 pre-S2의 중합 인혈청 알부민 수용체 활성을 저해시키는 결과는 HBV 중화항체에 대한 항원 결정부위와 중합 인혈청 알부민 수용체 부위가 같거나 공통서열을 가짐을 뒷받침한다. Pre-S2 region (amino acids 120-174) of hepatitis B virus surface antigen contains immunodominant and disulfide bond-independent epitopes. Using pre-S2 peptides (recombinant peptides 121-174/adw2, 134-170/adw2 and synthetic peptide 120-145/ayw), epitope and polyalbumin receptor region on pre-S2 were studied. Polyalbumin receptor assay and enzyme immunoassay provided evidences that the region of at least 12 residues (134-145) of pre-S2 was shared by epitope for an anti-pre-S2 monoclonal antibody and polyalbumin receptor. This region was shown to be hydrophilic and a possible ${\beta}$-sheet.

Recombinant Human Interleukin-2: I. Purification and Biochemical Characterization

윤혜영,최혜림,이명규,김승호,나도선,이선복,한문희,함경수,Yun, Hye-Young,Choi, Hye-Lim,Lee, Myeong-Kyu,Kim, Seung-Ho,Na, Doe-Sun,Lee, Sun-Bok,Han, Moon-H.,Hahm, Kyung-Soo 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.2

유전자 재조합 방법에 의해서 대장균으로 부터 재조합 인체 인터루킨-2 (rH IL-2, $Ser^{125}$-rH IL-2)를 과발현 시켰으며, 이 재조합 인체 인터루킨-2를 순수분리하여 그 생화학적 특성을 조사하였다. 분리 및 정제는 세포봉입체의 분리, 우레아 추출, 용해 및 젤여과 크로마토그라피 등을 사용하였으며, 정제된 인터루킨-2의 원형재현은 투석법을 사용하였다. 정제된 재조합 인터루킨-2의 순도는 전기영동 및 HPLC로 확인하였으며, 또한 결정을 얻음으로써 재확인하였다. 아미노말단 아미노산분석 및 아미노산배열순서를 일부 결정함으로서 정제된 재조합 인체 인터루킨-2가 천연 인터루킨-2와 동일한 일차구조를 갖고 있음을 확인하였다. Recombinant human interleukin-2 (rH IL-2, $Ser^{125}$-rH IL-2) was purified to apparent homogeneity from E. coli in high yield and characterized its biochemical properties for the establishment of preclinical screening system and therapeutic applications. The purification was carried out by methods involving isolation of inclusion body, urea extraction, solubilization and gel filtration chromatography. The renaturation of the product was achieved by extensive dialysis against the storage buffer. The purity was confirmed by SDS-PAGE and HPLC. Amino terminal amino acid analysis and partial amino acid sequence analysis showed that the primary structure of the recombinant protein (rH IL-2) was found to be identical with natural IL-2. The purity and the conformation of the rH IL-2 were al so confirmed by obtaining crystals of the recombinant protein.

B형 간염 바이러스 프리 - S2 펩티드의 분리 정제 및 중합 인혈청 알부민 수용체 검출법

윤혜영,한문희,함경수 ( Hye Young Yun,Moon H . Han,Kyung Soo Hahm ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

Recombinant pre-S2 peptide was purified to apparent homogeneity from E. coli by immunoaffinity chromatography using monoclonal antibody and studied for its binding characteristics with monoclonal antibody and pHSA (polymerized human serum albumin). A simple and sensitive assay method for detecting the activity of pHSA receptor on pre-S2 peptide of hepatitis B virus surface antigen (HBsAg) has been developed using pre-S2-β-galactosidase fusion protein. The assay can be used for determining pre-S2 or factors neutralizing pHSA receptor activity. The sensitivity of the assay is about 10 to 100 pmol of pre-S2.

칫솔질을 이용한 구강간호가 중환자실 환자의 구강상태 및 인공호흡기 관련 폐렴에 미치는 효과

윤혜영,이은숙,김정연,김향숙,김경애,김은성,손주온,김가희,김민정,김아람,박선정,추성미,손미정,하은진,오의금,Yun, Hye-Young,Lee, Eun-Sook,Kim, Jung-Yeon,Kim, Hyang-Sook,Kim, Kyung-Ae,Kim, Eun-Sung,Sohn, Joo-Ohn,Kim, Ka-Hee,Kim, Min-Jung,Kim, Ah-Ram 한국중환자간호학회 2011 중환자간호학회지 Vol.4 No.2

Purpose: The purpose of this study was to examine the effect of tooth-brushing oral care on oral health and ventilator-associated pneumonia of patients in Intensive Care Unit (ICU). Methods: A total of 74 participants were recruited from a medical, surgical, or neurosurgical ICU at S Hospital in Seoul, Korea, from September of 2010 to January of 2011. An experimental group (n= 36) received oral care with tooth-brushing while a control group (n=38) received oral care with cotton ball and gauze. In both group, the oral care was done three times a day at least one minute for 7 days. Oral health was examined by the Modified Oral Assessment Guide before the intervention and each night. Results: There is no difference between the groups in aspect of passage of time (p = .603), interaction between time and intervention (p =.300), the performance intervention (p = .766), and the incidence of VAP (p = .486). Conclusion: The effect of oral care with tooth-brushing on oral health and VAP was not different from that of usual oral care in ICU. However, further study is necessary due to high attrition in this study.

캐릭터 기반 트랜스미디어 스토리텔링 모델 연구 : 노드롭 프라이의 이론을 중심으로

윤혜영(Yun, Hye-Young) 한국만화애니메이션학회 2020 만화애니메이션연구 Vol.- No.61

본 연구의 목적은 노드롭 프라이의 서사 양식 이론을 바탕으로 캐릭터 기반의 트랜스미디어 스토리텔링 모델을 제안하는 데 있다. 노드롭 프라이의 서사 양식 이론은 캐릭터의 행동 능력과 시대 변화에 따른 양식의 변화를 기준으로 구조화된 이론으로 캐릭터의 트랜스미디어를 위한 모델을 구체화하는데 적합한 이론이다. 캐릭터 기반 트랜스미디어는 두 가지 유형으로 구분할 수 있다. 첫 번째는 각색 혹은 크로스미디어 스토리텔링에서 나타나는 축적형 캐릭터 트랜스미디어로 하나의 원형적 캐릭터가 시대가 변화함에 따라 새로운 캐릭터로 재창작되는 것을 의미한다. 세종 캐릭터가 대표적인 캐릭터인데 역사적 인물이자, 위인의 원형인 세종은 한국의 시대의 변화 대중의 요구에 따라 상위 모방을 거쳐 하위 모방과 아이러니 양식의 캐릭터로 재창작되었다. 두 번째는 이야기 세계 구성 형태에서 나타나는 확장형 캐릭터 트랜스 미디어로 하나의 캐릭터가 독립성을 갖고 이야기 세계 안의 여러 작품과 미디어를 옮겨다는 것을 의미한다. 마블 시네마틱 유니버스의 아이언맨 캐릭터가 대표적인 캐릭터이다. 어떤 캐릭터가 하나의 이야기에 국한되지 않고, 이야기 세계에서 여러 이야기 사이를 옮겨 다니기 위해서는 통일성 있는 정체성을 유지해야 한다. 아이언맨 캐릭터는 오이디푸스 콤플렉스라는 핵심적인 내적 문제를 가진 채 로맨스 양식과 하위 모방 양식의 캐릭터로 이야기 세계를 옮겨 다님으로써 캐릭터의 통일성을 유지하면 입체적인 캐릭터로 구현될 수 있었다. 네 가지 서사 양식에 따른 캐릭터의 변화는 하나의 캐릭터 안에 내재한 서로 다른 존재의 양식들이 여러 작품을 통해 각각 강조되는 것을 의미한다. 프라이의 서사 양식 이론을 중심으로 한 본 연구의 캐릭터 기반의 트랜스미디어 스토리텔링 모델은 트랜스미디어 캐릭터 창작을 위한 실제적인 방법론으로 활용될 수 있을 것으로 기대한다. The purpose of this study is to propose a character-based transmedia storytelling model based on Northrop Frye"s narrative mode theory. Northrop Frye"s narrative mode theory is a theory that is structured based on the character"s behavioral ability and the change of style according to the change of the times, and is a theory suitable to concrete a model for a character transmedia. Character-based transmedia can be classified into two types. The first is an accumulation-type character transmedia that appears in adaptation or cross-media storytelling, which means that an archetypal character is recreated as a new character as the times change. Sejong, a historical character and the archetype of a great man, was recreated as a character in the form of lower imitation and irony through upper imitation in accordance with the demands of the changing public in Korea. The second is the expandable character transmedia, which appears in the form of the story world composition, which means that one character has independence and transfers several works and media within the story world. The Iron Man character from Marvel Cinematic Universe is a representative character. A character is not limited to a single story, and in order to move between multiple stories in the story world, a unified identity must be maintained. The Iron Man character could be realized as a three-dimensional character by maintaining the unity of the character by moving the story world to a romance mode and a lower imitation mode character with the core internal problem of the Oedipus complex. The change of character according to the four narrative styles means that the different forms of existence within one character are emphasized through various works. The character-based transmedia storytelling model in this study, centered on Frye"s narrative mode, is expected to be utilized as a practical methodology for transmedia character creation.

도시 건축에서의 지역성과 역사성 -텐덴자, 이탈리아 건축운동(1965-1985)을 중심으로-

윤혜영 ( Yun Hye-young ),정문수 ( Jeong Moon-soo ) 부산외국어대학교 지중해지역원 2020 지중해지역연구 Vol.22 No.4

In today’s urban architecture, historicity and locality are impending issues both in preservation of modern buildings and construction of postmodern buildings. The duality implied by historicity and locality in urban architecture dates back to Tendenza, the Italian architectural movement that unfolded from the 1960s to the 1980s. This paper, a study on Tendenza, which suggested the importance of historicity and locality in urban architecture as a new architectural ‘trend’, is summarized as follows. First, the authors have searched out that Tendenza has succeeded in preservation of the history and the tradition asserted by Rationalism which occurred during the fascist period, the Neorealistic architecture and the Neoliberty movement after World War II, adapting these criteria to the contemporary time based on rationality. From this point of view, seeking to innovate modern architecture Tendenza can be evaluated as a Neorationalism movement. Second, the authors summarized that Tendenza emphasized thepreservation of the traces of history and the memory of the community in spaces and places. To realize them in architecture, it proposed a study on the tangible form and types of cities and buildings as a methodology. The so-called ‘Urban Typomorphology’ which is the methodology of Tendenza, emphasizes the history and traditions of communities, cities, and citizens rather than the nation-state, nation, and people. In addition, Tendenza provided the basis for the architectural movement that emphasized the duality, hybridity, and emotions that go beyond uniformity, unity, and reason which are the characteristics of modern architecture. In this aspect, Tendenza has a tendency to be regarded as a postmodern movement. Third, the authors have revealed that Tendenza has the characteristics of innovating modern architecture, while at the same time, it transcends the modern architecture towards ‘postmodern’ architecture. It is a bridge movement with the duality that connects the modern and postmodern architecture. The urban architecture of today has the impending issues, like locality and historicity similar to Tendenza’s.

『화차(火車)』에 투영된 “바람직한 자신(あるべき自分)”의 모습

윤혜영(Yun, Hye-Young) 한국일본문화학회 2018 日本文化學報 Vol.0 No.77

This paper focuses on the key concept of self-delusion in Miyabe Miyuki’s kasya (fire chariot) and examines the desirable self-concept of people in modern society through Sekine Shoko and Honda Ikumi. The characters in these works live by various self-delusion. In the novel, an anecdote is introduced to remind the reader of the actual state of a snake, an animal without any legs. This implies that it is important to acknowledge and accept the actual circumstances of one’s own life and identity. Specifically, Shoko is a woman who is miserable because of her wrong choices, “who bought a mirror that reflects her delusional self-concept instead of reflecting her actual self ”. However, Ikumi, who had been in a similar situation, is portrayed as a wise woman who lives a life that Shoko could have lived too. Ikumi, who accepts the reality of her own life and works hard to improve her circumstances, represents the desirable life of human beings in modern society.