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개비자나무 유효성분인 homoharringtonine의 in vitro 항암활성및 in vivo 만성 독성
유귀재,조철희,이건순,류재웅,채희정 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.2
Cell proliferation inhibitory effects of homoharringtonine (HHT), an active drug substance in Cephalotaxus koreana, against blood cancer cell line K562 were evaluated. In addition, in vivo chronic toxicity test with mouse was carried out. When K562 cell line was treated everyday for 9, 6, 3 days, IC50 values of HHT were determined as 0.27, 0.37, and 1.10 mM respectively. The anticancer activity of HHT was comparable to adriamycin, a known anticancer drug compound for blood cancer treatment. in vivo chronic toxicity test of the HHT, the number of red blood cell (RBC) showed no significant difference. From the analysis of the liver-functional enzymes in blood, all of liver damage related enzymes such as glutamate-oxalate-transferase (GOT), glutamate-pyruvatetransferase (GPT), cholesterol (Chol) and alkaline phosphatase (ALP) showed no significant change. However, from the histologic test, a neutrophil of the band type in liver tissue was observed. Cell proliferation inhibitory effects of homoharringtonine (HHT), an active drug substance in Cephalotaxus koreana, against blood cancer cell line K562 were evaluated. In addition, in vivo chronic toxicity test with mouse was carried out. When K562 cell line was treated everyday for 9, 6, 3 days, IC50 values of HHT were determined as 0.27, 0.37, and 1.10 mM respectively. The anticancer activity of HHT was comparable to adriamycin, a known anticancer drug compound for blood cancer treatment. in vivo chronic toxicity test of the HHT, the number of red blood cell (RBC) showed no significant difference. From the analysis of the liver-functional enzymes in blood, all of liver damage related enzymes such as glutamate-oxalate-transferase (GOT), glutamate-pyruvatetransferase (GPT), cholesterol (Chol) and alkaline phosphatase (ALP) showed no significant change. However, from the histologic test, a neutrophil of the band type in liver tissue was observed.
유귀재,이성동,인만진,Woo Ik Hwang,Kwang Seung Lee,Eun Sil Lee,Dong Chung Kim,채희정 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.4
Two types of sequential extraction process (SEP) for the recovery of bioactive components from Korean red ginseng were examined. As a SEP (normal SEP, nSEP), Korean red ginseng was sequentially treated using hot water and n-hexane for the successive extraction of water-soluble and lipid-soluble components. Also by changing the sequential order of solvents, an alternative SEP (reverse SEP, rSEP) in which n-hexane extraction was followed by hot water extraction, was proposed. Regardless of the sequential order of solvents, the recovery yield of acidic polysaccharide (AP) and crude saponin (CS) showed no significant change. On the other hand, in the rSEP, the lipid-soluble fraction was obtained from red ginseng with an enhanced recovery yield, four times higher than that in nSEP. Additionally, from dose-response assays to assess the effects of lipid-soluble components on the proliferation of human hepatoma (HepG2) and breast (MCF-7) cancer cell lines, it was found that hexane extract of red ginseng (HER) in rSEP has higher efficacy than the hexane extract of red ginseng marc (HERM) obtained in nSEP. This strongly suggested that rSEP would be a more attractive industrial process in terms of the efficacy of lipid-soluble extract as well as the recovery yield.
산겨릅나무 간 보호 화합물 salidroside의 추출조건 최적화
유귀재,최인욱,최상윤 한국생약학회 2022 생약학회지 Vol.53 No.4
Salidroside is a major phenolic glycoside of Acer tegmentosum (Aceraceae) and known to be a hepatoprotective compound. Extraction conditions are one of the most important factors to save time and cost from an economic point of view. For this reason, this study was conducted to optimize the extraction condition for maximum yield of salidroside. For optimization, three extraction factors such as ethanol concentration (%), extraction temperature (℃), and solvent to material ratio (mg/mL) were tested and optimized for maximum yield of salidroside using response surface methodology (RSM). The optimal condition was obtained as an ethanol concentration of 53.4%, an extraction temperature at 67.11℃ and a solvent to material ratio (mg/mL), 195.55 mg/ml. The salidroside yield under optimal conditions was found to be 1.59 mg/g dried samples, which were well-matched with the predicted value of 1.56 mg/g dried samples. These results will provide useful information about optimized extraction conditions for the development of salidroside as hepatoprotective therapeutics.
옻나무 (Rhus verniciflua Stokes) 추출물의 알코올대사 효소활성에 미치는 영향
유귀재(Guijae Yoo),김소영(Soyoung Kim),최아름(A Reum Choi),손민희(Min Hee Son),김동청(Dong Chung Kim),채희정(Hee Jeong Chae) 한국생물공학회 2009 KSBB Journal Vol.24 No.1
Alcohol oxidation activities and optimization of extraction conditions of Rrhus verniciflua Stokes (RVS) extract were evaluated for the development of a functional biomaterial for improving liver function. When alcohol oxidation activities of RVS was analyzed, the Rrhus verniciflua Stokes bark (RVSB) were higher than the Rrhus verniciflua Stokes heartwood (RVSH). Alcohol oxidation activity value of RVSB increased in a concentrationdependent manner. In the comparative analysis between Hovenia dulcis Thunb (HDT) and Alnus japonica Steud (AJS) which was reported as a alcohol oxidation material, alcohol oxidation activity is much higher than the others. The experimental conditions were optimized for alcohol oxidation-active components production from RVSB. The extraction conditions such as temperature, time, pH and particle size were performed. It was recommended to extract the alcohol oxidation-active components from RVSB by hot water (pH 7.0) at 85℃ for 8 hours.
꼬마모자반(Sargassum nipponicum)에서 유래한 유효성분의 최종당화산물에 의한 당뇨병성 신경병증 완화 효과
조치홍,유귀재,김민경,올파드위,정영성,이상훈 한국키틴키토산학회 2023 한국키틴키토산학회지 Vol.28 No.2
Advanced glycation end-products (AGEs) are compounds naturally produced in the body during the glycation reaction. Excessive AGEs bind to long-lived proteins such as collagen and accumulate in the body, leading to AGEsrelated diabetic complications. This study aimed to evaluate the anti-glycation ability and neuroprotective effect of 4- hydroxybenzaldehyde (4-HBA), 3-hydroxybenzaldehyde (3-HBA), and 2,5-dihydroxyphenylacetic acid (homogentisic acid; HGA) isolated from Sargassum nipponicum. The results confirmed that 4-HBA, 3-HBA, and HGA effectively inhibit the production of AGEs, prevent AGEs-collagen cross-link formation, and cleave pre-formed cross-links between AGEs and collagen, thereby exhibiting anti-glycation properties. Treatment with methylglyoxal (MGO) resulted in increased neuronal PC-12 cell death, intracellular reactive oxygen species production, and intracellular accumulation of MGO and AGEs. However, pretreatment with 4-HBA, 3-HBA, and HGA reduced AGEs production by suppressing MGO accumulation in neuronal PC-12 cells, which consequently lowered intracellular oxidative stress levels and exerted a neuroprotective effect. Notably, HGA exhibited a stronger binding affinity to the receptor for AGEs (RAGE) protein compared to MG-H1 and competitively inhibited AGEs-RAGE interaction, effectively downregulating RAGE protein expression in neuronal PC-12 cells. These findings highlight the chemical and structural properties of HGA derived from S. nipponicum, which effectively target the main mechanisms involved in AGEs-induced diabetic neuropathy. Therefore, HGA holds significant potential as a natural treatment for inhibiting AGEs formation.
장현석,강교빈,유귀재,권은아,윤준원,강병철,홍인기,김승현,성상현,조현종,장영표,양희정 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.6
Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z?ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high?performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z?ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC?UVD system was used to identify and quantify senkyunolide A and Z?ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long?term stability of the C. officinale samples in dark conditions at 4 and 25 °C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17%), and the recoveries were within the range of 95?105%. The contents were mostly independent of the geographical origin and species, and >10% of the compounds degraded after storage at 4 and 25 °C for 6 months. A method was developed to rapidly identify two chemical markers in cnidium rhizome with good linearity, precision, and accuracy using HPLC?UVD. These markers will be utilized for the quality control or evaluation of herbal preparations containing cnidium rhizome.
HPLC-UV를 이용한 천마의 Gastrodin과 Gastrodigenin의 동시분석법 확립
강교빈,전진범,유귀재,권은아,윤준원,강병철,김승현,성상현,장영표,양희정 한국생약학회 2017 생약학회지 Vol.48 No.3
Gastrodia elata has been widely used as a traditional medicinal herb. However, in Korean Pharmacopeia, neither a marker constituent nor an analytical procedure for G. elata has been established. In this study, we suggest gastrodin and gastrodigenin as marker constituents of G. elata, and propose an analytical procedure for simultaneous quantification of these constituents by high-performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV). The analytical method was validated for its linearity, precision, accuracy, and specificity. Based on the validated method, gastrodin and gastrodigenin in 14 commercial G. elata samples were quantified.