분자지표를 이용한 고려인삼의 유전적 특성 비교

신미란,조익현,정종욱,김영창,이승호,김장욱,현동윤,김동휘,김기홍,문지영,노봉수,강성택,이동진,방경환 韓國藥用作物學會 2013 한국약용작물학회지 Vol.21 No.6

The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derivedsimple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study,18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtainedfrom ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similaritycoefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of theresulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR mark-ers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer.The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginsengaccessions were classified into five groups by cluster analysis based on Nei’s genetic distances. Consequently, the results ofginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination ofKorean ginseng cultivars and breeding lines.

한국성인(韓國成人)의 사상체질음성분석기(絲狀體質音聲分析機)를 이용한 체질별(體質別) 음향특성(音響特性) 연구(硏究)

신미란,김달래,유준상,Shin, Mi-Ran,Kim, Dal-Rae,Yoo, Jun-Sang 사상체질의학회 2007 사상체질의학회지 Vol.19 No.3

1. Objectives The purpose of this study was to objectively diagnose American male and female's production of two vowels /a, i/ by Sasang Constitution. 2. Methods It was analyzed the constitutional characteristics of the American adults voices with PSSC-2004. of 134 cases of vowels /a, i/ with a duration of $2.5{\sim}3$ seconds were inputted in PSSC-2004 and analyzed into 40 factors. 3. Results and Conclusions 1) APQ In the male group's production of vowel /a/, the Soyangin's APQ(l), APQ(3) and APQ(4) were significantly high compared with those of Taeumin and Soeumin. 2) Shimmer In the male group's production of vowel /a/, Soeumin's Octave1 Shimmer was significantly low compared with that of Taeumin and Soeumin. In the male group's production of vowel /i/, Soeumin's D-Shimmer was significantly low compared with that of Taeumin and Soeumin. In the female group's production of vowel /a/, the Soyangin's C-Shimmer was significantly high compared with that of Taeumin and Soeumin. 3) Octave In the male group's production of vowel /a/, the Soyangin's Octave3, Octave4, Octave5, Octave6 and Octave1 Ratio were significantly high compared with those of Taeumin and Soeumin. In the male group's production of vowels /a, i/, the Soyangin's Octave4 was significantly high compared with that of Taeumin and Soeumin. 4) Energy In the male group's production of vowel /a/, the Soyangin's Time Domain Total Sum /Time Domain Count, Freq Domain Total Sum /cnt(0), 0k-4k Total Sum, Dev., A(A#, C, E, D#, E, F#) tot E, and A(C,, D#, F#) Dev. were significantly high compared with those of Taeumin and Soeumin. In the male group's production of vowel /i/, the Soyangin's Time Domain Total Sum /Time Domain Count, Freq Domain Total Sum /cnt(0) and 0k-4k Total Sum, Dev. were significantly high compared with those of Taeumin and Soeumin. 5) Peak In the male group's production of vowels /a/ and /i/,, the Soyangin's Peak1 Ratio was significantly low compared with that of Taeumin and Soeumin. In the male group's production of vowels /a/ and /i/,, the Soyangin's Peak10 Ratio, Time Domain Peak Total/Total Energy Sum, Time Domain Peak Dev. and Total/Total Dev. Sum were significantly high compared with those of Taeumin and Soeumin. 6) It is necessary to expand the research of the acoustic analysis of American and Korean to other countries in the diagnosis of the Sasang Constitution by using the voice characteristics.

도재 수복용 복합레진의 색 안정성에 관한 연구

신미란,조혜원,진태호,Shin Mee-Ran,Cho Hye-Won,Jin Tai-Ho 대한치과보철학회 1991 대한치과보철학회지 Vol.29 No.3

The purpose of this study was to investigate the color stability of 3 porcelain repair composite resins Twenty specimens of each composite resin were made and ten specimens were polished at 10 minutes after polymerization, and the others were polished at 48 hours after polymerization. For 60 days, the color characteristics were measured by fiber-optic colorimeter (Model Tc-6FX, Tokyo Denshoku Co.). The obtained results were as follows: 1. The changes of $a^*$ value and $b^*$ value in composite resin polished at 10min. after polymerization were greater than those of composite resins polished at 48 hrs. after polymerization. 2. The $a^*$ values of all composite resins were increased and the $b^*$ values were decreased. 3. The $L^*$ values were decreased in composite resin manufactured by K & B Co., which were polished at 10min. after polymerization, but increased in composite resin manufactured by K & M Co., polished at 48 hrs. after polymerization.

K-prism의 일부 문항 답가지에 대한 신뢰도 연구

신미란 사상체질의학회 2023 사상체질의학회지 Vol.35 No.2

Objectives The purpose of the current study was to examine the reliability of answers to some question items of K-prism using a test-retest approach. Methods We surveyed K-prism twice times at the interval of 6 months. We analyzed the reliability of test-retest for 55 items by performing McNemar analysis. Results As a result of the research on 55 items, 38 items showed an agreement of 80% or more and significant changes were shown in 6 items(P<0.05). There was a significant change in the answer on items No.4-3. 5-3, 11-3, 12-1(in the item of personality) and items No.20-3,4, 27-2(in the item of pathophysiological symptom(素證)). Among the personality items, the 'medium' response type affected the items showing significant response changes. Conclusion Through this study, most of the items of the k-prism questionnaire were reliable in test-retest. It may be necessary to consider revising the content and format for certain questions. -

코골이 대상자에게 비강사혈흡인요법의 안전성과 유효성을 평가하기 위한 다기관 임상시험

신미란,김희택,고호연,김영지,김정범,Shin, Mi-Ran,Kim, Hee-Taek,Ko, Ho-Yeon,Kim, Young-Ji,Kim, Jeung-Beum 대한한방안이비인후피부과학회 2017 한방안이비인후피부과학회지 Vol.30 No.2

Objectives : The study is to investigate clinical trial for efficacy and safety with acupuncture & suction in nasal cavity on snoring patients. Methods : 24 subjects were recruited from the patients received a hospital visit to the Semyung University Chungju oriental medical center and Jaechon oriental medical center from 1 October 2014 to 31 December 2014. According to the clinical protocol, we treated the patients with acupuncture and suction in the nasal cavity 8 times for 4 weeks. We compared their symptoms between before and after treatment. Results and Conclusions : 1. There was no significant decrease in the mechanical measurement of the reduction of snoring in the effectiveness of snoring patients, but there was a significant effect on the improvement of sleep quality and it was effective in improving the symptoms such as eye fatigue. 2. The safety of nasal treatment for snoring patients was normal in pre- and post-treatment Lab tests, and there was no adverse reaction before and after treatment. Acupuncture & suction in nasal cavity on snoring patients is efficient and safe.

CSL을 통한 음향특성과 사상체질간의 상관성 연구

신미란,김달래,Shin, Mi-ran,Kim, Dal-lae 사상체질의학회 1999 사상체질의학회지 Vol.11 No.1

1. 연구배경 사상인은 생리 및 병리 현상에 차이를 나타낼 뿐만 아니라 섭생법 및 치료법에서도 각기 다른 방법을 적용하고 있다. 그러므로 사상의학에 있어서 체질변증분야가 매우 중요한 과제라 하겠다. 동무는 사상인변증의 진단지표를 외형 심성 병증 등을 제시하였고, 많은 의가들에 의해 여러 가지 새로운 체질변증의 방법이 모색되어 임상에 시도 활용되고 있는 추세이나 체질변증의 객관성유지에 어려운 점이 많아서 학문의 발전에 적지 않은 난관이 따랐다. 최근에는 음성과학의 발달에 힘입어 음성의 특성을 가시적 수치적으로 객관화 할 수 있는 가능성이 높아졌다. 본 연구는 이러한 음성과학을 바탕으로 여러 의가들에 의해 제시되어 온 사상인의 음성적 특정을 각 개인의 사상체질과 직접적으로 연관성이 있다고 판단되는 공명주파수와 기본주파수 등 여러 음성학적인 pattern자료들을 모집단으로부터 획득하여 체질별로 객관적인 수치로 비교 분석함으로써 음성과 체질과의 관계에 대하여 살펴보고 개인의 성문에 의한 사상체질 판단의 기본자료로 활용하고자 하였다. 2. 연구방법 상지대한의과대학 남학생 132명과 기타8명을 대상으로 하였다. 음향특성과 체질과의 상관성에 관하여 문헌조사를 하였다. 설문지와 체질전문가에 의하여 연구대상자를 체질 판별하였다. 연구대상자의 음성특성에 대한 설문조사를 하였고 CSL로 Pitch. Formant Frequency. Energy, Time of reading composition 등을 분석하고 통계 처리하여 체질 판별을 시도하였다. 3. 연구결과 1) 설문조사결과 소음인은 음성이 낮고 완만하며 조용한 편으로, 소양인은 음성이 높고 밝으며 급하고 함부로 말을 하는 것으로, 태음인은 음성이 낮고 무거우며 완만하고 굵고 성량이 풍부한 것으로 나타났다. 2) 문장의 평균 발음 시간에서 태양인은 다른 체질에 비하여 유의성 있게 길었다. 몸무게에서 태음인은 다른 체질에 비하여 유의성 있게 높았다. Formant frequency 1에서는 태양인이, Bandwidth 1에서는 소음인과 태양인이 다른 체질에 비하여 유의성 있게 낮았으며, Bandwidth 2와 Formant frequency 5에서는 태양인이 유의성 있게 높았다. Pitch Maximum과 Pitch Maximum-Pitch Minimum에서는 소음인과 태양인이 유의성 있는 차이를 나타내었다. Energy Mean에서는 태양인과 태음인에서 유의성 있는 차이를 나타내었다. 3) Multi-dimensional 4-class minimum-distance classifier 분석결과 모든 항목으로 분석한 것 보다 체질별로 특정 항목을 갖고 분석한 것이 높은 일치를 나타내었다. SPSS/PC+프로그램에서 일원분산분석과 판별분석결과 네 체질사이에서의 정판별력보다 소양인을 제외한 3체질 사이에서의 정판별력이 높았다. CART모형에서 앞에서 제시된 다른 방법들보다 높은 예측율을 보이고 있다. 연구수행과정 중에 표준화, 녹음기술, 적적한 문장의 선택, 음성분석 항목선택, 통계기법, 적절한 알고리즘의 개발 등의 많은 문제점이 나타나고 있으며 관련분야의 보완과 연구진행이 지속적으로 있어야 할 것으로 사려 된다. 다각도로 진행되고 있는 사상의학에서의 객관화 노력과 통계적인 방법론들이 적용되고 체질진단에 있어 가장 최적의 알고리즘을 찾아낼 수 있다면, 정확한 체질 진단과 더불어 그에 따른 환자들의 치료와 약물처방에 있어서도 많은 도움을 줄 수 있으리라 생각된다. 성문과 음성특성의 분석을 통한 사상체질 분류검사방법은 사상체질의 객관화를 위한 하나의 보조적인 방법이 The purpose of this study is to help classifying Sasang Constitution through correlation with sound characteristic. This study was done it under the suppose that Sasang Constitution has correlation with sound spectrogram. The following result were obtained about correlation between sound spectrogram and Sasang Constitution by comparison and analysis 1. Soeumin answered his voice low tone, smooth and quiet in the survey. Soyangin answered his voice high, clear, fast and speaking random. Taeumin answered his voice low, thick and muddy. 2. Taeyangin was significantly slow compared with the others in the time of reading composition. Taeyangin was significantly slow compared with the others in Formant frequency 1. Taeyangin was significantly discriminated from Soeumin in Formant frequency 5. Taeyangin was significantly low compared with the others in Bandwidth 2. Soeumln was significantly low compared with Taeyangin in Pitch Maximum and Pitch Maximum-Pitch Minimum. Taeyangin was significantly high compared with the others in Energy mean. 3. In list of specification, the discrimination rate was higher than that by lists of 13 in the results of Multi-dimensional 4-class minimum-distance. The discrimination rate of three disposition except Soyangin was higher than that of four disposition in the results of One way ANOVA and Analysis of dis crimination in SPSS/PC+. In CART, the estimate rate of Sasang Constitution discrimination was higher than any other method. It is considered that there is a correlation between sound spectrogram and Sasang constitution according to the results. And method of Sasang constitution classification through sound spectrogram analysis can be one method as assistant for the objectification of Sasang constitution classification.

RAPD와 EST-SSR 마커를 이용한 고려인삼 품종과 육성계통의 유전적 다양성 분석 및 품종 판별

신미란,조익현,김영창,이승호,김장욱,현동윤,김동휘,김기홍,방경환,문지영,노봉수 한국약용작물학회 2013 한국약용작물학술대회 발표집 Vol.2013 No.10

사상체질별(四象體質別) 이소편(利小便) 약물(藥物)의 효능(效能)에 관한 연구(硏究)

신미란,김정렬,전종원,김달래,심규헌,Shin, Mee-Ran,Kim, Jung-Yul,Jeon, Jung-Won,Kim, Dal-Rae,Sim, Gyu-Heoun 사상체질의학회 2003 사상체질의학회지 Vol.15 No.3

The aim of this thesis is to examine the effect of the diuretics in the Sasang constitutional medicine those are not categorized to the diuretic in the established herbal medicine by the way of measuring the change of body edema and the quantity of body moisture through the inbody test, along with the urine volume taken for 24 hours 1. We medicated the diuretic suggested by the DongyiSuseBowon東醫壽世保元 into the tested group and measured the change of the urine volume, the quantity of body moisture and the degree of body edema. The results are the followings below. 1) Soeumin The change in the urine volume and body moisture showed no responsibility, but the degree of body edema decreased with the responsibility. 2) Soyangin All of the degree of body edema and the urine volume, and body moisture showed decrease. 3) Taeumin The urine volume and body moisture increased but showed no responsibility. Also the degree of body edema decreased within the normal limit which can not be accepted. 4) The medicine of the Sasang Constitutional Medicine can not be simply thought to be the diuretic, but it has the function that excretes the moisture which functions negatively in the body and causes edema, as well as the function of the adjustment of metabolism that preserves the normal amount of moisture. This is the good example which is the concept of the adjustment therapy suggested by The Sasang Constitutional Medicine 2. Through the results above, we can find that the diuretic suggested by The Sasang Constitutional Medicine functions in the way that is differently explained by the herbal medicine.

청폐사간탕(淸肺瀉肝湯)이 $CCl_4$로 유발(誘發)된 휜쥐의 간손상(肝損傷)에 마치는 영향(影響)

신미란,허운영,김달래,전종원,김정열,Shin, Mee-Ran,Heo, Woon-Yeong,Kim, DaI-Rae,Jeon, Jong-Weon,Kim, Jeong-Yeol 사상체질의학회 2005 사상체질의학회지 Vol.17 No.1

1. Objectives The present study was carried out to investigate the effects of Chungpyesagan-tang on the $CCI_4-induced$ Liver Damage in Rats. 2. Methods Sprague-Dawley rats were devided into 5 experimental groups : Normal, $NS+CCI_4(Solid$ extract of $CCI_4$ injection group after Normal Saline feed), $CP+CCI_4(Solid$ extract of $CCI_4$ injection group after Chungpyesagantang feed), $CCI_4+NS(Nomal$ Saline feed group after $CCI_4$ injection), $CCI_4+CP(Solid$ extract of Chungpyesagantang feed group after $CCI_4$ injection). Biochemical assays for serum enzyme activities such as AST, ALT, ALP, BUN, Creatinine, Uric Acid, Total Protein, Albumin, Total Cholesterol, Triglyceride, Glucose, and mRNA Revelation of Cytochrome p450 and activities such as LPO(Lipid Peroxidation), GSH(Glutathione), GST(Glutathione-S- Transferase), Glutathione Reductase, Glutathione Peroxidase, SOD(Superoxide Dismutase), Catalase, Hydroxyproline, and ${\beta}-Glucuronidase$ were performed. 3. Results 1) $CP+CCI_4$ showed significantly lower relation of Cytochrome p450 than $NS+CCI_4$. 2) As to LPO Hydroxyproline, $CCI_4+CP$ showed significantly lower activity than $CCI_4+NS$. 3) As to GSH GST Glutathione Peroxidase Catalase, $CP+CCI_4$ showed higher activity than $NS+CCI_4$, $CCI_4+CP$ showed significantly higher activity than $CCI_4+NS$. 4) As to Glutathione Reductase SOD, $CCI_4+CP$ showed significantly higher activity than $CCI_4+NS$. 5) As to ${\beta}-Glucuronidase$, $CP+CCI_4$ showed signficantly lower activity than $NS+CCI_4$. 4. Conclusions Chungpyesagantang has the recovering effects on the $CCI_4-induced$ Liver Damage.

캠프 형식의 입원 치료를 받은 소아 아토피피부염 환자 6례

신미란,오진아,이동녕,Shin, Mi-Ran,Oh, Jin-Ah,Lee, Dong-Nyung 대한한방소아과학회 2014 대한한방소아과학회지 Vol.28 No.2

Objectives This study investigated the effect of camping style Hospitalization program for atopic dermatitis children patients. Methods 6 patients were admitted for camping style hospitalization program. The program includes acupuncture, herbal medicine, examination, education, psychotherapy, dressing, exercise. The patients were analyzed by Severity Scoring Atopic Dermatitis (SCORAD) index and measured skin pH value (pH). Also, patients evaluated themselves by writing an atopic dermatitis diary which consists of diet, emotion, severity of pruritus, and sleep loss. Checked questionnaires which consists of atopic dermatitis, self treatment methods and each program satisfaction. Results After 7 days of hospitalization program, the patient's SCORAD index scores were decreased and most of the patient's symptoms were improved. Conclusions The hospitalization program could be helpful for atopic dermatitis children patients.