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Mycoplasma pneumoniae의 분리 및 항생제 감수성 검사(III)
장명웅,김광혁,박인달,송갑영,김성원,이은영,김문찬,조명훈,김규언,최충언,박선영,조현장,Chang Myung-Woong,Kim Kwang-Hyuk,Park In-Dal,Song Gap-Young,Kim Sung-Won,Lee Eun-young,Kim Moon-Chan,Cho Myung-Hoon,Kim Kyu-Earn,Choi Choong-Eon,Park Se 한국생명과학회 2005 생명과학회지 Vol.15 No.3
2002년 2월부터 2005년 2월까지 성인 및 소아 호홉기질환자 994명의 상기도 도말물에서 M. pneumoniae 균주를 분리하고, 분리 균주의 ciprofloxacin, ofloxacin, minecline, tetracycline, sparfloxacin, josamycin, erythromycin에 대한 감수성 검사를 실시하였으며, 분리된 균주의 235 rRNA domain II와 V에서 erythromycin저항성 변이가 일어났는가를 PCR과 유전자 염기서열분석으로 erythromycin에 감수성인 M. pneumonine균주의 염기서열과 비교분석하여 확인하였다. 호흡기질환자에서 M. pneumoniae의 분리율은 123/994$(12.4\%)$이었으며, 분리된 M. pneumoniae 균주의 minocycline, sparfloxacin, tetracycline, ciprofloxacin, ofloxacin, josamycin, erythromycin MIC 범위는 각각 $0.015\~0.25,\;0.06\~0.5,\;0.06\~0.5,\;0.25\~0.5,\;0.25\~0.5,\;0.015\~8.0,\;0.015\~8.0{\mu}m$이었다. 분리 동정된 M. pneumoniae 균주 중에서 erythromycin에 저항성인 균주가 60주$(48.8\%)$였으며, 모두가 ribosomal protein L4 영역과 23S rRNA domain V에 내성변이가 일어났으며, 이 중 2균주는 23S rRNA domain II에도 변이가 일어난 균주도 있었다. 국내에서 분리되는 M. pneumoniae균주의 $48\%$가 erytomycin에 저항성인 균주이므로 앞으로 이 균에 의한 폐렴의 치료에 주의가 요구된다. The 994 throat swabs obtained from 688 adults and 306 children patients with respiratory diseases were examined for Mycoplasma pneumoniae infection by culture method. Antimicrobial susceptibilities of the resulting 123 M. pneumoniae isolates were evaluated by testing minimum inhibitory concentrations (MICs) of erythromycin, minocycline, tetracycline, josamycin, sparfloxacin, ofloxacin, and ciprofloxacin by a broth micro-dilution method. The erythromycin resistant strains of M. pneumoniae was determined above $1.0{\mu}g/ml$ of MIC for erythromycin. The erythromycin resistant strains of M. pneumoniae was confirmed resistant gene mutation of the portions of genes 23S rRNA (domain II and V), and ribosomal protein 14 and L22 by PCR amplified and their nucleotide sequenses were compared to those of the susceptible strain M129. The isolation rate of M. pneumoniae was $12.9\%$ (89/688) for the adults and $11.1\%$ (34/306) for the children. The $MICs_{90}$ of the M. pneumoniae isolates were $0.12{\mu}g/ml$ for minocycline, $0.25{\mu}g/ml$ for sparfloxacin, $0.5{\mu}g/ml$ for ciprofloxacin, ofloxacin, and tetracycline, respectively, and $2.0{\mu}g/ml$ for josamycin and erythromycin, respectively. The isolation rate of erythromycin resistant M. pneumoniae from patients was $49.4\%\;(44/89)$ for the adults, $47.1\%\;(16/34)$ for children, and $48.8\%\;(60/123)$ for the total. No mutation could be detected in the ribosomal protein L22 region, but all strains were mutated in the ribosomal protein L4 as two point mutation M144V. Two point mutations in domain V of 23S rRNA were selected in the presense of erythromycin resistant M. pneumoniae isolates, such as one strain was G2057C mutant, two strains were A2059C mutants, three strains were C2611G mutants, four strains were A2058C mutants, five strains were A2058T mutants, twenty strains were A2059G mutants, and twenty-five strains were A2058G mutants, respectively. These results show that erythromycin was not the most active compound against M. pneumoniae infection in Korea and clinical studies of macrolides in human patients are demanded.
호흡기질환 환자로부터 분리된 Mycoplasma pneumoniae의 tetracycline과 erythromycin에 대한 저항성 변이
장명웅,박인달,김광혁,송갑영,김성원,Chang Myung-Woong,Park In-Dal,Kim Kwang-Hyuk,Song Gap-Young,Kim Sung-Won 한국생명과학회 2005 생명과학회지 Vol.15 No.6
2002년 2월부터 2005년 4월까지 호흡기질환 환자로부터 분리된 M. pneumeniae 123 균주의 tetracycline과 erythromycin에 대한 MIC 범위는 각각 $0.5\~1.0$, and $0.5\~512{\mu}/ml$ 이었다. 분리된 M. pneumoniae 123 균주에서 plasmid DNA는 확인되지 않았다. 분리된 M. pneumoniae 123 균주 중에서 57($46.3\%$) 균주가 tetracycline에 저항성인 tetM유전자를 가지고 있었으며, 235 rRNA domain V에 erythromycin에 저항성 변이를 일으킨 균주가 60($48.8\%$)이었다. erythromycin에 저항성 변이를 일으키지 않은 63균주 중에서 tetM 유전자를 가지고 있는 균주는 36($57.1\%$)이었으며, erythromycin에 저항성 변이를 일으킨 60균주 중에서 21($35.0\%$ 균주가 tetM 유전자를 가지고 있었다. 본 연구로써 국내에서 tetracycline과 erythromycin에 대한 저항성 M. pneumoniae 균주의 분리율이 외국에 비하여 높으며, M. pneumoniae 감염의 치료에 erythromycin이 일차 선택제가 될 수 없으므로 이에 대한 범국가적 조사가 필요하다고 생각된다. One hundred and twenty three strains of Mycoplasma pneumoniae were isolated from patients with respiratory diseases from February 2002 to April 2005 in Busan, Korea. The MICs of tetracycline and erythromycin up to $90\%$ of the 123 M. pneumoniae isolates tested were $0.5\~1.0$, and $0.5\~512{\mu}/ml$, respectively. Plasmid DNA was not isolated from all of the M. pneumoniae isolates. Out of 323 strains of M. pneumoniae, 57 ($46.3\%$) stains contain tetM gene on their chromosomal DNA, and 60 ($48.8\%$) strains were mutated in domain V of 23S rRNA for erythromycin resistance. Out of 63 strains of M. pneumoniae which were not mutated in domain V of 235 rRNA for erythromycin resistance, 36 ($57.1\%$) strains contained tetM gene, and out of 60 strains of M. pneumoniae which were mutated in domain V of 23S rRNA for erythromycin resistance, 21 ($35.0\%$) strains contained tetM gene. These results suggest that the isolation rate of erythromycin and tetracycline resistant M. pneumoniae is higher than those of other countries, and erythromycin and tetracycline are not first choice drug for M. pneumoniae infection in Korea, and it need confirm by a nationwide surveilance of antimicrobial resistance.
가성낭을 동반한 만성 췌장염 환자에서 발생한 비장경색 및 비장파열 1 예
조용구(Yong Gu Cho),임영찬(Young Chan Lim),한병호(Beoung Ho Han),김충기(Chung Kee Kim),임홍섭(Hong Seub Lim),심영웅(Young Woong Sim),송갑영(Gap Young Song) 대한소화기학회 2001 대한소화기학회지 Vol.38 No.6
Splenic infarction is not common disease which is caused by occlusion of the main splenic artery or any of its branches. It is mainly caused by emboli that arise from cardiovascular disease, but sometimes by regional thrombosis associated with leukemia, myeloproliferative disorder, sickle cell anemia, pancreatitis, portal hypertension, splenic torsion, septicemia and drugs, etc. Splenic infarction accompained by chronic pancreatitis is most likely due to splenic vein thrombosis, surrounding tissue fibrosis accompanied with splenic vessel occlusion, and intrasplenic penetration of pancreatic pseudocyst. Recently, we experienced a case of splenic infarction and rupture in a 46-year-old male who had been diagnosed as chronic pancreatitis with pseudocyst. (Korean J Gastroenterol 2001;38:457-460)
위 질환 환자들에서 분리된 Helicobacter pylori 의 공포화 독소 ( Vacuolating Toxin ) 생성
김충기(Chung Kee Kim),정승수(Sung Soo Jeong),이겸철(Gyum Cheol Lee),서대홍(Dae Hong Suh),송갑영(Gap Young Song),최경현(Kyung Hun Choi),장명웅(Myung Woong Chang) 대한내과학회 1998 대한내과학회지 Vol.54 No.5
Objectives: This study was carried out to survey the prevalence of Helicobacter pylori infection and the incidence of vacuolating toxin producing H pylori. A further aim of this study was to evaluate the quantitative assay for cell vacuolation on the basis of the rapid uptake of neutral red dye by vaculoes of the cells. Methods: We studied the gastric biopsy specimens of patients with 154 cases of gastritis, 74 cases of gastric ulcer, and 167 cases of gastric cancer and in 44 cases of healthy persons, One of the biopsy specimen was placed into a CLOtest plate for rapid urease test and the other one of the biopsy spcimen was inoculated on Brain Heart Infusion blood agar for culture. The culture supernatant of isolated H. pylari was serially diluted with BHI broth. After 24 hour incubation of cultured RK-13 cells treated with the culture supernatgnt of H. pylori, cytoplasmic vacuolation of the cells were observed microscopically. Results: The positivity of urease test and the rate of isolation of K pylori from urease positive gastric biopsy materials were 34.1% and 93.3% in healthy person, 55.8% and 70.9% in gastritis, 60.8% and 71.1% in gastric ulcer, and 56.3% and 96.8% in gastric cancer. The isolation rate of H. pylari from patients between 20 and 39 years old was 16.8%, far patients between 40 and 59 years old it was 51.9%, and for patients above 60 years old it was 31.2%. The isolation rate of the vacuolating toxin producing H pylori from gastric biopsy specimens was 66.7% in a healthy person, 76.6% in gastritis, 79.4% in gastric ulcer, and 80% in gastric cancer. Conclusions: The isolation rate of H. pylori from the patients with gastric diseases is higher than the rate of H. pylori from healthy persons, but the isolation rate of the vacuolating toxin producing H. pylori is not different between the patients with gastric diseases and healthy persons. The titers of vacuolating toxin produced by some H, pylori isolated from the patients with gastric diseases are higher than those from healthy persons.
Mycoplasma pneumoniae의 신속 동정과 분리균의 항생제 감수성 검사
장명웅(Myung-Woong Chang),김광혁(Kwang-Hyuk Kim),박인달(In-Dal Park),강경희(Kyung-Hee Kang),공은희(Eun-Hee Kong),정만홍(Man-Hong Jung),송갑영(Gap-Young Song),조성환(Sung-Hwan Jo),조동희(Dong-Whee Cho),한병호(Byung-Ho Han),김성원(Sung 대한미생물학회 2003 Journal of Bacteriology and Virology Vol.33 No.3
서대홍,임홍섭,김충기,이겸철,조용구,정승수,심영웅,송갑영 대한감염학회 1998 감염 Vol.30 No.2
우상복부 동통, 발열과 오한 그리고 의식 둔화를 주소로 내원한 당뇨 환자에서 Klebsiella pneumoniae에 의한 가스형성 간농양을 균배양 검사를 통하여 진단하고 항생제 투여와 경피적 농양 배액술을 통해 증상의 호전을 보인 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Gas-forming pyogenic liver abscess is an uncommon, life-threatening, necrotizing infection that is usually found in poorly-controlled diabetic patients. Herein, we report a case of gas-forming liver abscess caused by Klebsiella pneumoniae, as proven by aspirated pus culture, in a 70-year old woman with diabetes mellitus. The patient was successfully managed with broadspectrum antibiotics and transhepatic percutaneous drainage and was discharged after 3 weeks of hospitalization. In conclusion, strict control of diabetes mellitus is the most important factor in the prevention of gas-forming pyogenic liver abscess.