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위 질환 환자들에서 분리된 Helicobacter pylori 의 공포화 독소 ( Vacuolating Toxin ) 생성
김충기(Chung Kee Kim),정승수(Sung Soo Jeong),이겸철(Gyum Cheol Lee),서대홍(Dae Hong Suh),송갑영(Gap Young Song),최경현(Kyung Hun Choi),장명웅(Myung Woong Chang) 대한내과학회 1998 대한내과학회지 Vol.54 No.5
N/A Objectives: This study was carried out to survey the prevalence of Helicobacter pylori infection and the incidence of vacuolating toxin producing H pylori. A further aim of this study was to evaluate the quantitative assay for cell vacuolation on the basis of the rapid uptake of neutral red dye by vaculoes of the cells. Methods: We studied the gastric biopsy specimens of patients with 154 cases of gastritis, 74 cases of gastric ulcer, and 167 cases of gastric cancer and in 44 cases of healthy persons, One of the biopsy specimen was placed into a CLOtest plate for rapid urease test and the other one of the biopsy spcimen was inoculated on Brain Heart Infusion blood agar for culture. The culture supernatant of isolated H. pylari was serially diluted with BHI broth. After 24 hour incubation of cultured RK-13 cells treated with the culture supernatgnt of H. pylori, cytoplasmic vacuolation of the cells were observed microscopically. Results: The positivity of urease test and the rate of isolation of K pylori from urease positive gastric biopsy materials were 34.1% and 93.3% in healthy person, 55.8% and 70.9% in gastritis, 60.8% and 71.1% in gastric ulcer, and 56.3% and 96.8% in gastric cancer. The isolation rate of H. pylari from patients between 20 and 39 years old was 16.8%, far patients between 40 and 59 years old it was 51.9%, and for patients above 60 years old it was 31.2%. The isolation rate of the vacuolating toxin producing H pylori from gastric biopsy specimens was 66.7% in a healthy person, 76.6% in gastritis, 79.4% in gastric ulcer, and 80% in gastric cancer. Conclusions: The isolation rate of H. pylori from the patients with gastric diseases is higher than the rate of H. pylori from healthy persons, but the isolation rate of the vacuolating toxin producing H. pylori is not different between the patients with gastric diseases and healthy persons. The titers of vacuolating toxin produced by some H, pylori isolated from the patients with gastric diseases are higher than those from healthy persons.
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서대홍,임홍섭,김충기,이겸철,조용구,정승수,심영웅,송갑영 대한감염학회 1998 감염 Vol.30 No.2
우상복부 동통, 발열과 오한 그리고 의식 둔화를 주소로 내원한 당뇨 환자에서 Klebsiella pneumoniae에 의한 가스형성 간농양을 균배양 검사를 통하여 진단하고 항생제 투여와 경피적 농양 배액술을 통해 증상의 호전을 보인 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Gas-forming pyogenic liver abscess is an uncommon, life-threatening, necrotizing infection that is usually found in poorly-controlled diabetic patients. Herein, we report a case of gas-forming liver abscess caused by Klebsiella pneumoniae, as proven by aspirated pus culture, in a 70-year old woman with diabetes mellitus. The patient was successfully managed with broadspectrum antibiotics and transhepatic percutaneous drainage and was discharged after 3 weeks of hospitalization. In conclusion, strict control of diabetes mellitus is the most important factor in the prevention of gas-forming pyogenic liver abscess.
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최환주,심영웅,서대홍,김춘섭,송갑영,임홍섭,김충기,이겸철 대한내과학회 1997 대한내과학회지 Vol.53 No.2
Hepatoma is one of the most common malignant disease among cancers that occur in Korea. Recently, according ta developing imaging diagnostic technology and non surgical treatment the hepatoma is easily detected in early diagnosis and appropriate treatment. From this point of view, the histologic pattern of hepatoma is markedly important. This is the first reported case of sclerosing hepatocellular carcinoma, which is characterized by intense fibrosis, in which the tubular neoplastic structures are embedded. The incidence of sclerosing hepatocellular carcinoma is very rare. Therefore the rare histologic pattern of hepatoma might be introduced by many studies and reports. We recently experienced a case of sclerosing hepatocellular carcinoma.
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