http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 서정수 ( Jung-soo Seo ) (검색결과 85 건)
- 무료
- 기관 내 무료
- 유료
-
넙치 (Paralichthys olivaceus) 뇌로부터 phospholipase D 활성 억제 단백질의 정제 및 특성 규명
서정수,김은희,황은영,김남득,김동선,이형호,정준기,SEO Jung-Soo,KIM Eun-Hi,HWAWG Eun-Young,KIM Nam Deuk,KIM Dong Sun,LEE Hyung-Ho,CHUNG Joon-Ki 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4
본 연구에서는 넙치, Pafalichthys olivaceus 뇌 조직에서 인지질가수분해효소 D (phospholipase D, PLD) 활성의 특성 규명 및 이 활성을 억제하는 단백질을 분리 정제하여 그 특성을 규명하였다. 넙치 뇌 조직에서 PLD 활성이 관찰되었으며, 이 활성은 포스파티딜 이노시톨 비스인산염 (phosphatidyl-inositol 4,5-bisphos-phate, $PIP_2$)에 대해서 의존성을 나타내었으나, ADP-rebosylation factor (ARF)에 의해서는 영향을 받지 않았다. PLD 억제물은 넙치 뇌 조직의 세포질 분획물을 사용하여 여러 종류의 칼럼을 통하여 분리 정제하였고, 그 억제물의 분자크기 및 기작의 특성을 규명하였다. 마지막 chromatography을 통하여 여섯 개의 억제를 나타내는 분획물을 얻었으며, 이 중 두 개의 분획물인 IIA IIB는 $PIP_2$-phosphatase activities를 나타내었다. 이 중 IIA 분획물은 면역화학적 분석을 통하여 inositolpolyphosphate 5-phosphatase family로 알려져 있는 신경말단 단백질인 synaptojanin으로 동정되었다. 그리고 IIB fraction은 Superose 12 gel filtration chromatography을 통하여 158-kDa의 크기로 확인되었으며, 이것은 면역화학적 분석을 통하여 synaptojanin과는 별개의 단백질로 판명되었다. 또한, IIB 분획물은 $PIP_2$ phosphatase activity 확인 실험에서 대사산물로서 phosphatidylinositol phosphate (PIP)만을 생성하였다. 이 결과는 IIB 분획물이 $PIP_2$의 4혹은 5 위치의 인산 (phosphate) 중 어느 하나만을 선택적으로 가수분해시킨다는 것을 암시한다. 이상의 연구 결과들을 종합하여 보면, 넙치 뇌 조직에는 다양한 형태의 $PIP_2$-phosphatases가 존재하며, 이들은 $PIP_2$-의존적인 PLD 활성의 억제조절과정에서 중요한 역할을 할 것으로 사료된다. Flounder brain cytosol contains protein inhibitors that markedly inhibit the activity of partially purified brain membrane phospholipase D (PLD) which is dependent on phosphatidylinositol 4,5-bisphosphate ($PIP_2$) but insensitive to ADP-ribosylation factor (ARF), The PLD inhibitors have been enriched through several chromatographic steps and characterized with respect to size and mechanism of inhibition. Sequential chromatography of the brain cytosol yielded six inhibitor fractions, Two (IIA and IIB) of six inhibitor fractions showed the $PIP_2$-phosphatase activities. IIA was identified as synaptojanin, a nerve terminal protein that has known to be a member of the inositolpolyphosphate 5-phosphatase family, by immunoblot analysis. IIB showed an apparent molecular mass of 158 kDa by Superose 12 gel filtration chromatography and was immunologically distinct from synaptojanin. IIB hydrolyzed $PIP_2$, yielding only phosphatidylinositol phosphate (PIP) as product, suggesting that IIB hydrolyzes only one phosphate from either the 4- or 5-position of PI (4,5)$P_2$. These studies demonstrate that the existence of multiple $PIP_2$-phosphatases have been implicated in the negative regulation of $PIP_2$-dependent PLD activity within flounder brain.
-
서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG) 한국수산해양교육학회 2018 수산해양교육연구 Vol.30 No.6
Scuticociliate are one of the main parasite affecting the flounder aquaculture industry, causing significant economic losses in Korea. Aquatic formalin has been approved to treat against external parasitic infestation in flounder aquaculture pond. To investigate metabolic effect after treating the parasitic agent aquatic formalin in scuticociliate Miamiensis avidus, the transcriptome analysis was conducted. Through exposing with low concentration(10~50 ppm) of formalin for 96 hr, parasite trancriptomes were sequenced cDNA libraries in the GX Plex sequencer. A total of million read for control and formalin treated group were assembled in 187,210 and 177,959 contigs, respectively. After normalization and assembling with house keeping gene(β-actin), transcripts were assigned to biological processes and functions after annotation in Gene Ontology(GO). Specifically, 10 exclusive transcripts were up- or down-regulated for control and formalin treated group, respectively. Our results provide valuable genetic information for further analysis of the biological responses mechanism of aquatic formalin exposure in M. avidus.
-
넙치에 대한 Erythromycin thiocyanate의 단일 근육투여에 따른 약물동태학적 특성
서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON) 한국수산해양교육학회 2018 수산해양교육연구 Vol.30 No.6
The study was aimed at investigating the pharmacokinetics of erythromycin thiocyanate(EM) in the cultured olive flounder(Paralichthys olivaceus) following single intramuscular administration, using LC/MS/MS following. After single administration of EM(5 and 10 mg/kg body weight) by intramuscular injection in olive flounder(332±20 g, 20±0.5℃), the EM concentration in the serum was determined at 1, 3, 6, 9, 24, 72, 120, 168, 264, 360, 504 and 720 h post-dose, respectively. The kinetic profile of EM intramuscular administration in serum were analyzed fitting to a non-compartment model by WinNonlin program. The residual pattern of EM was immediately decreased on after single administration. Concentrations of EM were not measurable at 168 hours (7 days) after intramuscular administration in all samples. The results of this study could be used for determining withdrawal time and efficacy dose of EM for the treatment of bacterial diseases in culturing olive flounder.
-
-
-
서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jun ),정승희 ( Sung Hee Jung ),김명석 ( Myoung Sug Kim ),박명애 ( Myoung Ae Park ),이철호 ( Chul Ho Lee ),한명철 ( Myoung Chul Han ),김진우 ( Jin Woo Kim ),지보영 ( Bo Young Jee ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1
The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was 98±27, 103±27, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was 63±18 and 108±17, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature, 4℃, -20℃, -80℃). As a result, it was necessary to store at -20℃ for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.
-
생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향
서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG),김진도(Jin-Do KIM),정승희(Sung-Hee JUNG),김나영(Na-Young KIM),지보영(Bo-Young JEE),박명애(Myoung-Ae PARK) 한국수산해양교육학회 2015 수산해양교육연구 Vol.27 No.6
The effects of lightyellow sophora, Sophora flavescens extract were tested on non-specific immune response and a disease resistance of olive flounder, Paralichthys olivaceus. For feeding trial, weight gain of fish fed different treated groups were not significant differences. Serum analysis of treated and control group did not demonstrated any differences. Lysozyme activity in the kidney and spleen of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase, respectively. In addition, phagocytic activity of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase compared to the control group. Histopathology of the liver and kidney tissue of the administrated group for 4<SUP>th</SUP>, 8<SUP>th</SUP> and 12<SUP>th</SUP> week showed no particular signs of tissue degeneration. The treated group was higher than control group by analyzing the relative percent survival (RPS) of the experimental challenge of Edwardsiella tarda. Therefore, the lightyellow sophora showed effective in preventing fish-pathogenic edwardsiellosis for a certain period of time.
-
복합 생약재 열수추출물 투여에 따른 넙치 세균성 질병에 대한 질병저항성
서정수(Jung Soo SEO),전은지(Eun Ji JEON),권문경(Mun Gyeong KWON),황지연(Jee Youn HWANG),정승희(Sung Hee JUNG),김나영(Na Young KIM),지보영(Bo Young JEE),박명애(Myoung Ae PARK) 한국수산해양교육학회 2016 수산해양교육연구 Vol.28 No.6
The heat extracts of six kinds of medicinal herbs (Scutellaria baicalensis, Sophora flavescens, Citrus unshiu pericarpium, Lonicera japonica, Perilla frutescens, Benincasa hispida) were tested for non-specific immune response and disease resistance effects related with fish diseases on olive flounder, Paralichthys olivaceus. The preventive effects of 6 herbal mixtures against bacterial disease on cultured flounder were examined follow as feeding EP absorbed with the heat extract of six kinds of medicinal herbs. For feeding trial for 12th week, weight gain and serum analysis of fish fed various groups were not significant differences. Lysozyme activity of the 0.01 % treated group on 4th week showed significant increase. Histopathology of the administrated group for feeding period showed no particular signs of tissue degeneration. At 0.01% oral experimental group, relative percent survival (RPS) were only 50% to 75% for four weeks and eight weeks group by intraperitoneal injection with E. tarda. The results suggest that heat extracts of six kinds of medicinal herbs (0.01%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen.
-
경구투여에 의한 양식산 넙치내 Erythromycin의 약물동태학적 특성 분석
서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),정승희(Sung-Hee JUNG),박명애(Myung-Ae PARK),김나영(Na-Young KIM) 한국수산해양교육학회 2014 수산해양교육연구 Vol.26 No.2
The pharmacokinetics of erythromycin (EM) after oral administration was studied in the cultured olive flounder, Paralichthys olivaceus, using LC/MS/MS. After single- or multiple-dose administration of EM (50, 100, 200 ㎎/㎏ body weight and 50 ㎎/㎏ for 5 days) by oral route in olive flounder (350±40 g, 22±0.5℃), the concentration in the serum was determined at 1, 3, 6, 9, 24, 72, 120, 168, 264, 360, 504 and 720 h post-dose. The kinetic profile of absorption, distribution and elimination of EM in serum were analyzed fitting to a two-compartment model by WinNonlin program. The area under the concentration-time curve (AUC), maximum concentration (Cmax), time for maximum concentration (Tmax) following oral administration of 50, 100 and 200 ㎎/㎏ b.w. and 50 ㎎ for 5 days. EM was 165.3 hr<SUP>*</SUP>㎍/㎖ (Cmax, 34.63 ㎍/㎖; Tmax, 1.56 hr), 212.8 hr<SUP>*</SUP>㎍/㎖ (Cmax, 60.38 ㎍/㎖; Tmax, 3.99 hr), and 592.37 hr<SUP>*</SUP>㎍/㎖ (Cmax, 63.01 ㎍/㎖; Tmax, 4 hr), respectively. The results of this study related to dosage and μㆍwithdrawal times could be used for prescription of EM in field for the treatment of bacterial diseases in olive flounder.
-
서정수(Seo, Jung-Soo) 계명대학교 산학연구소 2010 經營經濟 Vol.43 No.3
Globalization has been accompanied by a rapid expansion of trade in goods and services and foreign direct investment(FDI). The rapid expansion of trade in intermediate goods and services over last 10 years has highlighted firms are different in productivity and in other aspects such as size. Multinational firm"s sourcing strategy has become complex due to the fragmentation of production processes, and firms face the choice of production location and organizational forms. Mushrooming evidence that firms are heterogeneous and also that they fragment production processes spurred the development of "new new" trade theories based on firm heterogeneity and incomplete contract.
DBpia연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
