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생약재 황금 뿌리 열수추출물의 넙치 투여시 질병저항성에 미치는 영향
지보영 ( Bo Young Jee ),서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),이은혜 ( Eun Hye Lee*,),최희정 ( Hee Jung Choi ),김진도 ( Jin Do Kim ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ) 수산생명의학회 2012 수산생명의학회지 Vol.25 No.1
Effects of various concentration of skullcap Scutellaria baicalensis in the diets on a nonspecific immunity and a disease resistance of olive flounder were investigated. After feeding trial, weight gain of fish fed 0.05% skullcap immersed group was higher than that of fish fed 0, 0.1 and 1% skullcap diet but no significant differences were observed among the experimental groups. Furthermore, no significant differences in hematological indices of olive flounder were found among the experimental groups. Lysozyme activity in the serum and kidney of the administrated group(0.05% skullcap immersed group) was significantly higher than the control group. In addition, the chemiluminescent(CL) responses of head kidney leucocytes from the 0.05% skullcap immersed group was significantly higher(P<0.05) than the control group. In the histological results, the 1% skullcap immersed group appeared to have the detrimental effects for fish health. In a challenge experiment with Edwardsiella tarda(GY-01) and Streptococcus iniae(FT5228), relative percent survival (RPS) in the 0.05% skullcap immersed group was higher than the control group injected with E. tarda(GY-01) at 4th and 8th weeks. The results suggest that the skullcap extract (0.05%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen such as E. tarda.
양식 넙치 폐사피해 및 수산생물용 의약품 사용량 모니터링
지보영 ( Bo Young Jee ),신기원 ( Ki Won Shin ),이대욱 ( Dae Wook Lee ),김영재 ( Young Jae Kim ),이무근 ( Mu Kun Lee ) 수산생명의학회 2014 수산생명의학회지 Vol.27 No.1
The monitoring was performed to survey the mortalities and medications occurred in the inland aquaculture farms of olive flounder in South Korea from May to October, 2012. Both of the indirect inquiry for entire inland farms and the sample survey for selected farms were carried out. The aquatic organism disease inspectors, who have the national licenses for the diagnosis and prevention of aquaticorganism diseases and have close relationship with the farms, investigated the rates and causes of mortalities according to the standard manual. The cumulative mortalities rate by the indirect inquiry on 565 farms, was calculated to 27.18%, and the mortalities rate by infectious diseases was 22.64%. Otherwise, the mortalities rate by sample survey on 60 farms was 25.50%, 19.33% of them were caused by infectious diseases. The high mortality rates were recorded by scuticociliatosis, non-infectious loss, streptococcosis, VHS, artificial eliminations, vibriosis and gliding bacterial disease. Streptococcosis and non-infectious mortality caused to serious loss in productivity and economy of the farms, because of their outbreaks in the flounder groups over 600 g. The monitoring of medications in the selected farms revealed that formalin for the treatment of external parasites, such as scuticociliates, was the most commonly used drugs in the farms. As the antibiotic medications, amoxicillin and florfenicol for streptococcosis, and oxytetracycline and neomycin expecting wide antibacterial spectrum, were frequently prescribed.
우리나라 양식 쥐치, Stephanolepis cirrhifer에서 분리된 비브리오의 특성
지보영 ( Bo Young Jee ),방종득 ( Jong Deuk Bang ),조미영 ( Mi Young Cho ),김진우 ( Jin Woo Kim ),원경미 ( Kyoung Mi Won ),조영아 ( Young Ah Cho ) 수산생명의학회 2011 수산생명의학회지 Vol.24 No.2
An epidemic was occurred in Stephanolepis cirrhifer during acclimation in laboratory tanks (water temperature was about 17℃). Diseased fish showed an unique external sign, large cloudy skin surface. We investigated the cause of the disease, and isolated one pure cultured bacterium, that was identified as Vibrio anguillarum by biochemical analysis and sequence analysis of the 16S rRNA and recA genes. During the outbreak, about 30% of S. cirrhifer showed the large cloudy skin surface, and approximately 10% of the stocked fish died. Fish were effectively cured by treatment with oxolinic acid.
조미영,지보영,박명애,표희동,Cho, Mi Young,Jee, Bo Young,Park, Myoungy-Ae,Pyo, Heedong 한국해양생명과학회 2016 한국해양생명과학회지 Vol.1 No.2
The success or failure of aquaculture heavily depends on how effectively to manage aquatic animal diseases. The paper is to estimate the direct effects of economic benefits on aquatic animal disease prevention program using economic surplus analysis. The economic benefits include changes in consumer and producer surplus owing to a reduction effect of aquatic animal mortality and the consumption recovery effect. The annual changes in producer surplus for flounder, rockfish and others cultured is estimated as 2.33 billion won totally (0.87 billion won, 1.01 billion won and 0.45 billion won respectively) and the annual changes in consumer surplus 10.15 billion won totally (6.36 billion won, 1.85 billion won and 1.94 billion won respectively). Therefore, total annual economic benefit is 12.5 billion won.
바이러스성 출혈성 패혈증 바이러스 NV 단백질에 의한 glucokinase 전사 활성의 억제
조미영(Mi Young Cho),황지연(Jee Youn Hwang),지보영(Bo Young Ji),박명애(Myoung Ae Park),성미소(Mi So Seong),김소영(So Young Kim),정예은(Ye Eun Jung),정재훈(Jae Hun Cheong),최영현(Yung Hyun Choi) 한국생명과학회 2016 생명과학회지 Vol.26 No.12
바이러스성 출혈성 패혈증 바이러스(VHSV)는 넙치를 포함한 어류 양식의 막대한 피해를 일으키는 바이러스 병원체이며, VHSV가 생성하는 6개의 바이러스 단백질들 중에서 NV 단백질이 병원성에 관여하는 것으로 알려져 있다. VHSV-감염 넙치를 이용한 전사체 마이크로 어레이의 선행 분석 결과에 의하면 VHSV 감염이 해당과정 효소들의 mRNA 발현을 억제함으로써 넙치 세포에서 ATP 생성을 감소시켰음을 알 수 있었다. 이들 결과를 토대로, 본 연구에서는 VHSV NV 단백질이 해당과정 효소인 glucokinase의 발현에 미치는 영향을 검토하였다. 본 연구의 결과에 의하면, NV 단백질은 넙치 세포에서 glucokinase의 mRNA 발현을 감소시켰으며, 새롭게 동정한 glucokinase의 유전자 프로모터의 활성 실험결과, NV 단백질이 glucokinase의 프로모터 활성을 저해함을 알 수 있었다. 이와 같은 작용 결과들로 인하여 VHSV NV 단백질의 발현이 세포 내로의 포도당 흡수 또한 감소시켰다. 이러한 결과들은 VHSV NV 단백질이 유전자 발현의 전사 수준에서 음성적으로 해당과정의 효소 발현을 조절함을 의미하며, 결국 세포 내 에너지의 결핍으로 넙치의 폐사로 이어질 가능성을 보여주는 것이다. The viral hemorrhagic septicemia virus (VHSV), which belongs to the Novirhabdovirus genus of the Rhabdoviridae family, is a viral pathogen that causes severe losses in the olive flounder farming industry. Among six encoding VHSV proteins, the non-virion (NV) protein has been shown to have an impact on virulence. In our previous studies, transcriptomics microarray analysis by using VHSV-infected olive flounder showed that VHSV infection significantly down-regulated the mRNA expression of glycolytic enzymes. In addition, VHSV NV protein variants decreased the intracellular ATP level. Based on these results, we have tried to examine the effect of VHSV NV protein on glycolytic enzyme glucokinase expression, which phosphorylates glucose to glucose 6-phosphate. Our results indicated that the NV protein significantly decreased the mRNA expression of glucokinase in olive flounder HINAE cells. Furthermore, the NV protein played a negative role in the promoter activation of glucokinase. Furthermore, glucose uptake was effectively inhibited by VHSV infection and NV protein expression in olive flounder HINAE cells. These results suggest that the VHSV NV protein negatively regulates glycolytic enzyme expression by a transcription level and eventually leads to gradual morbidity of olive flounder through cellular energy deprivation. The present results may be useful for the prevention and diagnosis of VHSV infection in olive flounder.