넙치 (Paralichthys olivaceus) 뇌로부터 phospholipase D 활성 억제 단백질의 정제 및 특성 규명

서정수,김은희,황은영,김남득,김동선,이형호,정준기,SEO Jung-Soo,KIM Eun-Hi,HWAWG Eun-Young,KIM Nam Deuk,KIM Dong Sun,LEE Hyung-Ho,CHUNG Joon-Ki 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4

Flounder brain cytosol contains protein inhibitors that markedly inhibit the activity of partially purified brain membrane phospholipase D (PLD) which is dependent on phosphatidylinositol 4,5-bisphosphate ($PIP_2$) but insensitive to ADP-ribosylation factor (ARF), The PLD inhibitors have been enriched through several chromatographic steps and characterized with respect to size and mechanism of inhibition. Sequential chromatography of the brain cytosol yielded six inhibitor fractions, Two (IIA and IIB) of six inhibitor fractions showed the $PIP_2$-phosphatase activities. IIA was identified as synaptojanin, a nerve terminal protein that has known to be a member of the inositolpolyphosphate 5-phosphatase family, by immunoblot analysis. IIB showed an apparent molecular mass of 158 kDa by Superose 12 gel filtration chromatography and was immunologically distinct from synaptojanin. IIB hydrolyzed $PIP_2$, yielding only phosphatidylinositol phosphate (PIP) as product, suggesting that IIB hydrolyzes only one phosphate from either the 4- or 5-position of PI (4,5)$P_2$. These studies demonstrate that the existence of multiple $PIP_2$-phosphatases have been implicated in the negative regulation of $PIP_2$-dependent PLD activity within flounder brain.

포르말린 폭로에 따른 스쿠티카충의 유전체 발현 특성

서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG) 한국수산해양교육학회 2018 수산해양교육연구 Vol.30 No.6

Scuticociliate are one of the main parasite affecting the flounder aquaculture industry, causing significant economic losses in Korea. Aquatic formalin has been approved to treat against external parasitic infestation in flounder aquaculture pond. To investigate metabolic effect after treating the parasitic agent aquatic formalin in scuticociliate Miamiensis avidus, the transcriptome analysis was conducted. Through exposing with low concentration(10~50 ppm) of formalin for 96 hr, parasite trancriptomes were sequenced cDNA libraries in the GX Plex sequencer. A total of million read for control and formalin treated group were assembled in 187,210 and 177,959 contigs, respectively. After normalization and assembling with house keeping gene(β-actin), transcripts were assigned to biological processes and functions after annotation in Gene Ontology(GO). Specifically, 10 exclusive transcripts were up- or down-regulated for control and formalin treated group, respectively. Our results provide valuable genetic information for further analysis of the biological responses mechanism of aquatic formalin exposure in M. avidus.

제주 및 완도지역 양식넙치에서 잔류 동물용의약품 모니터링

서정수(Jung Soo SEO),조희성(Hee Sung JO),정아름(Ah-Reum JEONG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee Youn HWANG),황성돈(Seong Don HWANG),정지민(Ji Min JEONG),이지훈(Ji-Hoon LEE) 한국수산해양교육학회 2019 수산해양교육연구 Vol.31 No.5

Residue levels in randomly collected olive flounder muscles were monitored in 2015-2018. Fish were obtained from fish farms located in Jeju and Wando regions to monitor 45 veterinary antibiotic drugs. For analysis, sensitive methods which adopt simultaneously detection of multiple agents were used: high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) detection. A total of 1,200 samples were analyzed, and antibiotic residues were detected in 15 Jeju samples (1.25%) and 45 Wando samples (3.75%). None of them contained residues exceeding respective maximum residue limits (MRLs) set by the Korean Food Code. Although the present monitoring result identifies safe status overall, current surveillance efforts over antibiotic use need to be maintained by continuous monitoring.

넙치에 대한 Erythromycin thiocyanate의 단일 근육투여에 따른 약물동태학적 특성

서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON) 한국수산해양교육학회 2018 수산해양교육연구 Vol.30 No.6

The study was aimed at investigating the pharmacokinetics of erythromycin thiocyanate(EM) in the cultured olive flounder(Paralichthys olivaceus) following single intramuscular administration, using LC/MS/MS following. After single administration of EM(5 and 10 mg/kg body weight) by intramuscular injection in olive flounder(332±20 g, 20±0.5℃), the EM concentration in the serum was determined at 1, 3, 6, 9, 24, 72, 120, 168, 264, 360, 504 and 720 h post-dose, respectively. The kinetic profile of EM intramuscular administration in serum were analyzed fitting to a non-compartment model by WinNonlin program. The residual pattern of EM was immediately decreased on after single administration. Concentrations of EM were not measurable at 168 hours (7 days) after intramuscular administration in all samples. The results of this study could be used for determining withdrawal time and efficacy dose of EM for the treatment of bacterial diseases in culturing olive flounder.

미꾸라지 간으로부터 포스포리파아제 C델타 단백질의 생화학적 특성

서정수 ( Jung Soo Seo ),임상욱 ( Sang Uk Lim ),김나영 ( Na Young Kim ),이상환 ( Sang Hwan Lee ),오현석 ( Hyun Suk Oh ),이형호 ( Hyung Ho Lee ),정준기 ( Joon Ki Chung ) 한국어병학회 2005 한국어병학회지 Vol.18 No.1

단보 : 양식산 넙치, Paralichthys olivaceus 식세포의 식작용 활성에 미치는 chloramphenicol의 영향

서정수 ( Jung Soo Seo ),정소정 ( So Jung Jung ),이상환 ( Sang Hwan Lee ),김나영 ( Na Young Kim ),엄혜경 ( Hye Kyung Eom ),허민도 ( Min Do Huh ),정현도 ( Hyun Do Jung ),정준기 ( Joon Ki Chung ) 한국어병학회 2004 한국어병학회지 Vol.17 No.3

생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향

서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG),김진도(Jin-Do KIM),정승희(Sung-Hee JUNG),김나영(Na-Young KIM),지보영(Bo-Young JEE),박명애(Myoung-Ae PARK) 한국수산해양교육학회 2015 수산해양교육연구 Vol.27 No.6

The effects of lightyellow sophora, Sophora flavescens extract were tested on non-specific immune response and a disease resistance of olive flounder, Paralichthys olivaceus. For feeding trial, weight gain of fish fed different treated groups were not significant differences. Serum analysis of treated and control group did not demonstrated any differences. Lysozyme activity in the kidney and spleen of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase, respectively. In addition, phagocytic activity of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase compared to the control group. Histopathology of the liver and kidney tissue of the administrated group for 4<SUP>th</SUP>, 8<SUP>th</SUP> and 12<SUP>th</SUP> week showed no particular signs of tissue degeneration. The treated group was higher than control group by analyzing the relative percent survival (RPS) of the experimental challenge of Edwardsiella tarda. Therefore, the lightyellow sophora showed effective in preventing fish-pathogenic edwardsiellosis for a certain period of time.

유카추출물과 didecyldimethylammonium chloride를 주성분으로 하는 살균소독제 아쿠아 팜세이프의 어류병원체에 대한 살균 효과

서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),황지연 ( Jee Youn Hwang ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),이성민 ( Sung Min Lee ),이은혜 ( Eun Hye Lee ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

In this study, the disinfectant efficacy of Aqua farmsafe®, composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe® possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe® against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua framsafe® will be able to reduce fish diseases.

양식산 넙치에서 Enrofloxacin의 잔류

서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),이은혜 ( Eun Hye Lee ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),지보영 ( Bo Young Jee ),김나영 ( Na Young Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.1

Enrofloxacin is one of the normally used flouroquinolones in mammalian and fish but its withdrawal time and studies were remain obscure. The residual contents of enrofloxacin in fish muscle were analyzed by using HPLC-FLD. More than 0.1 mg/kg of ENR was detected in muscle tissues and the residues were found over 1 year after treatment. The concentration of ENR in Paralichthys olivaceus was not affected by water temperature and lasted for an extended amount of time. The spike recoveries of ENR in the muscle tissue ranged from 78% to 85%. From this results, we need the prescription by veterinarian or aquatic organism disease inspector in ENR usage to assure safety of fish. Future research is required to determine the recommendation dose of ENR for side effects and safety.

단보 : 한국산 연어의 아니사키스형 유충의 감염현황

서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jun ),정승희 ( Sung Hee Jung ),김명석 ( Myoung Sug Kim ),박명애 ( Myoung Ae Park ),이철호 ( Chul Ho Lee ),한명철 ( Myoung Chul Han ),김진우 ( Jin Woo Kim ),지보영 ( Bo Young Jee ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1

The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was 98±27, 103±27, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was 63±18 and 108±17, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature, 4℃, -20℃, -80℃). As a result, it was necessary to store at -20℃ for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.