복합 생약재 열수추출물 투여에 따른 넙치 세균성 질병에 대한 질병저항성

서정수(Jung Soo SEO),전은지(Eun Ji JEON),권문경(Mun Gyeong KWON),황지연(Jee Youn HWANG),정승희(Sung Hee JUNG),김나영(Na Young KIM),지보영(Bo Young JEE),박명애(Myoung Ae PARK) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.6

복합 생약재 열수추출물(6종 혼합)을 농도별 기간별로 사료에 흡습시켜 12주 동안 넙치에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 복합 생약재 열수추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았으며 혈액생화학적인 , 결과도 넙치의 생리에는 아무런 영향을 미치지 않았다. 라이소자임 활성은 0.01%의 복합 생약재 열수추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 복합 생약재 열수추출물의 투여에 따른 넙치 장기의 병리조직에 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병 저항성은 0.01% 복합 생약재 열수추출물 농도에서 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 넙치에 복합 생약재 열수추출물을 투여시에는 어류의 비특이적 면역력 증강 및 질병 저항성 증강을 나타내는 것으로 사료되었다. The heat extracts of six kinds of medicinal herbs (Scutellaria baicalensis, Sophora flavescens, Citrus unshiu pericarpium, Lonicera japonica, Perilla frutescens, Benincasa hispida) were tested for non-specific immune response and disease resistance effects related with fish diseases on olive flounder, Paralichthys olivaceus. The preventive effects of 6 herbal mixtures against bacterial disease on cultured flounder were examined follow as feeding EP absorbed with the heat extract of six kinds of medicinal herbs. For feeding trial for 12th week, weight gain and serum analysis of fish fed various groups were not significant differences. Lysozyme activity of the 0.01 % treated group on 4th week showed significant increase. Histopathology of the administrated group for feeding period showed no particular signs of tissue degeneration. At 0.01% oral experimental group, relative percent survival (RPS) were only 50% to 75% for four weeks and eight weeks group by intraperitoneal injection with E. tarda. The results suggest that heat extracts of six kinds of medicinal herbs (0.01%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen.

포르말린 폭로에 따른 스쿠티카충의 유전체 발현 특성

서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),지보영(Bo-Young JEE),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG) 한국수산해양교육학회 2018 水産海洋敎育硏究 Vol.30 No.6

Scuticociliate are one of the main parasite affecting the flounder aquaculture industry, causing significant economic losses in Korea. Aquatic formalin has been approved to treat against external parasitic infestation in flounder aquaculture pond. To investigate metabolic effect after treating the parasitic agent aquatic formalin in scuticociliate Miamiensis avidus, the transcriptome analysis was conducted. Through exposing with low concentration(10~50 ppm) of formalin for 96 hr, parasite trancriptomes were sequenced cDNA libraries in the GX Plex sequencer. A total of million read for control and formalin treated group were assembled in 187,210 and 177,959 contigs, respectively. After normalization and assembling with house keeping gene(β-actin), transcripts were assigned to biological processes and functions after annotation in Gene Ontology(GO). Specifically, 10 exclusive transcripts were up- or down-regulated for control and formalin treated group, respectively. Our results provide valuable genetic information for further analysis of the biological responses mechanism of aquatic formalin exposure in M. avidus.

경구투여에 의한 양식산 넙치내 Erythromycin의 약물동태학적 특성 분석

서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),정승희(Sung-Hee JUNG),박명애(Myung-Ae PARK),김나영(Na-Young KIM) 한국수산해양교육학회 2014 水産海洋敎育硏究 Vol.26 No.2

The pharmacokinetics of erythromycin (EM) after oral administration was studied in the cultured olive flounder, Paralichthys olivaceus, using LC/MS/MS. After single- or multiple-dose administration of EM (50, 100, 200 ㎎/㎏ body weight and 50 ㎎/㎏ for 5 days) by oral route in olive flounder (350±40 g, 22±0.5℃), the concentration in the serum was determined at 1, 3, 6, 9, 24, 72, 120, 168, 264, 360, 504 and 720 h post-dose. The kinetic profile of absorption, distribution and elimination of EM in serum were analyzed fitting to a two-compartment model by WinNonlin program. The area under the concentration-time curve (AUC), maximum concentration (Cmax), time for maximum concentration (Tmax) following oral administration of 50, 100 and 200 ㎎/㎏ b.w. and 50 ㎎ for 5 days. EM was 165.3 hr<SUP>*</SUP>㎍/㎖ (Cmax, 34.63 ㎍/㎖; Tmax, 1.56 hr), 212.8 hr<SUP>*</SUP>㎍/㎖ (Cmax, 60.38 ㎍/㎖; Tmax, 3.99 hr), and 592.37 hr<SUP>*</SUP>㎍/㎖ (Cmax, 63.01 ㎍/㎖; Tmax, 4 hr), respectively. The results of this study related to dosage and μㆍwithdrawal times could be used for prescription of EM in field for the treatment of bacterial diseases in olive flounder.

단보 : 유카추출물과 didecyldimethylammonium chloride를 주성분으로 하는 살균소독제 아쿠아 팜세이프의 어류병원체에 대한 살균 효과

서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),황지연 ( Jee Youn Hwang ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),이성민 ( Sung Min Lee ),이은혜 ( Eun Hye Lee ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

본 연구에서는 4급 암모늄에 속하는 didecyldimethylammonium chloride (DDAC)와 유카추출물 (Yucca extract)의 합제인 아쿠아 팜세이프®의 어류 질병 병원체에 대한 살균효과를 확인하기 위하여 농림수산검역 검사본부의 소독제 효력시험 중 세균 및 바이러스 소독제 효력시험에 따라 수행하였다. 소독제와 병원체를 증류수, 경수 그리고 유기물 조건에서 반응시킨 후, 중화액을 이용하여 중화시킨 다음, 중화된 용액을 배지 및 주화세포에서 접종시켜 세균의 성장 및 세포 변성효과를 통해 소독제의 효력배수를 결정하였다. 본 연구의 결과, 아쿠아 팜세이프®는 식중독을 일으키는 공시균주 살모넬라균 (S. typhimurim)에 비하여, 어병세균이 30~40배 이상 높은 희석농도에서 소독 효과를 나타내었으며, VHSV에 대하여서는 2,000~3,600배의 유효한 효력배수를 나타내었다. 따라서, 향후, 야외적용시험을 통해 실제 적용에 따른 효과를 확인할 필요가 있을 것으로 사료된다. In this study, the disinfectant efficacy of Aqua farmsafe®, composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe® possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe® against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua framsafe® will be able to reduce fish diseases.

상시개방 개폐기 양단 전압 위상차를 이용한 배전계통의 루프운전 구간 전압 계산 방법

서정수(Jung-Soo Seo),임일형(Il-Hyung Lim),박종호(Jong-ho Park),신용학(Yong-Hak Shin),최면송(Myeon-Song Choi) 대한전기학회 2016 대한전기학회 학술대회 논문집 Vol.2016 No.7

양식산 넙치에서 Enrofloxacin의 잔류

서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),이은혜 ( Eun Hye Lee ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),지보영 ( Bo Young Jee ),김나영 ( Na Young Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.1

Enrofloxacin is one of the normally used flouroquinolones in mammalian and fish but its withdrawal time and studies were remain obscure. The residual contents of enrofloxacin in fish muscle were analyzed by using HPLC-FLD. More than 0.1 mg/kg of ENR was detected in muscle tissues and the residues were found over 1 year after treatment. The concentration of ENR in Paralichthys olivaceus was not affected by water temperature and lasted for an extended amount of time. The spike recoveries of ENR in the muscle tissue ranged from 78% to 85%. From this results, we need the prescription by veterinarian or aquatic organism disease inspector in ENR usage to assure safety of fish. Future research is required to determine the recommendation dose of ENR for side effects and safety.

미꾸라지 간으로부터 포스포리파아제 C델타 단백질의 생화학적 특성

서정수 ( Jung Soo Seo ),임상욱 ( Sang Uk Lim ),김나영 ( Na Young Kim ),이상환 ( Sang Hwan Lee ),오현석 ( Hyun Suk Oh ),이형호 ( Hyung Ho Lee ),정준기 ( Joon Ki Chung ) 한국어병학회 2005 한국어병학회지 Vol.18 No.1

단보 : 양식산 넙치, Paralichthys olivaceus 식세포의 식작용 활성에 미치는 chloramphenicol의 영향

서정수 ( Jung Soo Seo ),정소정 ( So Jung Jung ),이상환 ( Sang Hwan Lee ),김나영 ( Na Young Kim ),엄혜경 ( Hye Kyung Eom ),허민도 ( Min Do Huh ),정현도 ( Hyun Do Jung ),정준기 ( Joon Ki Chung ) 한국어병학회 2004 한국어병학회지 Vol.17 No.3

생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향

서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG),김진도(Jin-Do KIM),정승희(Sung-Hee JUNG),김나영(Na-Young KIM),지보영(Bo-Young JEE),박명애(Myoung-Ae PARK) 한국수산해양교육학회 2015 水産海洋敎育硏究 Vol.27 No.6

고삼 뿌리 열수 추출물을 농도별로 넙치 사료에 흡습시켜 12주 동안 어류에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 고삼 추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았다. 혈액화학적인 조사 결과에서도 고삼 첨가로 인한 넙치의 생리에는 아무런 영향을 나타내지 않았다. 라이소자임 활성은 0.05%의 고삼 추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 백혈구의 식세포 활성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서 유의적으로 증가되었다. 병리학적 결과를 통하여 고삼 추출물을 농도별로 넙치에 투여하였을때 병리조직학적 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병저항성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서만 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 고삼뿌리 열수 추출물은 넙치에 대한 비특이적 면역력 증강 및 질병 저항성 증강에 효과가 있는 것으로 사료되었다. The effects of lightyellow sophora, Sophora flavescens extract were tested on non-specific immune response and a disease resistance of olive flounder, Paralichthys olivaceus. For feeding trial, weight gain of fish fed different treated groups were not significant differences. Serum analysis of treated and control group did not demonstrated any differences. Lysozyme activity in the kidney and spleen of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase, respectively. In addition, phagocytic activity of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase compared to the control group. Histopathology of the liver and kidney tissue of the administrated group for 4<SUP>th</SUP>, 8<SUP>th</SUP> and 12<SUP>th</SUP> week showed no particular signs of tissue degeneration. The treated group was higher than control group by analyzing the relative percent survival (RPS) of the experimental challenge of Edwardsiella tarda. Therefore, the lightyellow sophora showed effective in preventing fish-pathogenic edwardsiellosis for a certain period of time.

배전자동화 중앙장치에서 필터링 알고리즘을 통한 고장표시 오류 개선방법

서정수(Jung-Soo Seo),김형승(Hyung-Seung Kim),임성일(Sung-Il Lim),최면송(Myeon-Song Choi),이승재(Seung-Jae Lee) 대한전기학회 2015 전기학회논문지 Vol.64 No.10

In this paper, an filtering method of FI(Fault Indication) information generated by FRTU(Feeder Remote Terminal Unit)s is proposed for the present DAS(Distribution Automation System). In order to find fault area, correct FI information should be generated. But when a single line-to-ground fault occurs, FI information is generated in downside of the fault in some circumstance because existing FI algorithm considers only magnitude. These wrong FI information can be removed by changing existing algorithm. An improved algorithm considers both the direction of zero-sequence current and the phase of three-phase current&voltage. But many FRTUs are distributed in DAS and Changing the algorithm all of FRTU will spend a lot of time and cost. On the other hand, an filtering algorithm proposed in this paper can substitute for it. The filtering algorithm also considers both the direction of zero-sequence current and the phase of three-phase current&voltage. In case study, the proposed method has been shown the reasonability in filtering the fault indication information.