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        • KCI등재

          자연산 점몰개, Squalidus multimaculatus Hosoya et Jeon의 새인두흡충, Clinostomum complanatum (Rudolphi, 1814) 감염

          박명애 ( Myoung Ae Park ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),최희정 ( Hee Jung Choi ),전은지 ( Eun Ji Jeon ),지보영 ( Bo Young Jee ),이완옥 ( Wan-ok Lee ),우승호 ( Sung Ho Woo ),이은혜 ( Eun Hye Lee ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2

          경상북도 영덕군의 하천에 서식하는 점몰개 spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon)의 근육에서 새인두흡충 Clinostomum complanatum (Rudolphi, 1814)의 피낭유충이 발견되었다. 감염된 점몰개는 "yellow grub"인 기생충의 피낭유충으로 인해 표면이 울퉁불퉁하게 보였으며, 수면위에 힘없이 유영하였고 일부 폐사한 개체도 발견되었다. 분리된 탈낭유충의 형태는 혓바닥 모양이었으며, 크기는 2.85~5.89 mm×0.8~1.99 mm였다. 어체당 1~98 개의 피낭유충을 분리하였으며 평균 감염수는 25개로 나타났다. 특히, 성어크기인 평균 7 cm 정도의 점몰개는 100% 감염되어 있었다. 본 연구는 한국의 자연수계에 서식하는 어류에서 C. complanatum 의해 질병이 발생하고 폐사가 일어난 첫 보고이다. The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by "yellow grub" showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm × 0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.

        • KCI등재

          Amoxicillin trihydrate의 단독 경구투여 및 정맥투여에 따른 뱀장어, Anguilla japonica 체내 약물동태학적 특성

          전은지 ( Eun Ji Jeon ),서정수 ( Jeong Soo Seo ),김진도 ( Jin Do Kim ),정승희 ( Sung Hee Jung ),김명석 ( Myoung Sug Kim ),황지연 ( Jee Youn Hwang ),박명애 ( Myoung Ae Park ),지보영 ( Bo Young Jee ),김진우 ( Jin Woo Kim ),김이청 ( Y 한국어병학회 2010 한국어병학회지 Vol.23 No.3

          아목시실린(Amoxicillin trihydrate; Amox)을 뱀장어(평균 체중 220±10 g)에 1일 1회 경구투여(40 및 80 mg/kg body weight) 및 정맥투여(1 mg/kg)한 다음, 경시적(0시간∼720시간)으로 혈장내 Amox의 잔류농도를 분석하였다. 40 및 80 mg/kg 농도로 경구투여한 모든 시험구에서 투여 6시간째 각각 3.3±0.5 및 3.4±0.1 ㎍/㎖로 최대혈중농도를 나타내었다. Amox의 모든 시험구는 투여 720시간째 혈중에서 검출되지 않았다. Amox의 경구투여방법에 따른 뱀장어 체내 약물 혈중농도 측정결과를 바탕으로 WinNonlin program을 이용한 2-compartment model로 하여 Amox의 흡수, 배설, 반감기 등 약물동태학적 매개변수(parameter)를 조사하였다. 2-compartment model을 이용한 분석을 통하여 40 및 80 mg/kg Amox를 경구 투여한 경우, 혈장농도-시간곡선하 면적(AUC)은 각각 464 및 667 ㎍·h/㎖, 혈중최고농도의 도달시간(Tmax)은 2.1 및 3.6 hr, 혈중최고농도(Cmax)는 3.04 및 3.4 ㎕/㎖로 계산되었다. 1 mg/kg Amox을 정맥 투여한 경우, 혈장농도-시간곡선하 면적(AUC)은 748 ㎍·h/㎖, 혈중최고농도(Cmax)는 4.2 ㎕/㎖로 계산되었다. 1일 1회 단독으로 40 및 80 mg/kg Amox를 각각 경구 투여시의 뱀장어내 생체내이용율(F%; bioavailability)은 각각 1.6, 1.1%로 매우 낮게 나타났다. 이러한 결과는 아목시실린을 삼수화물 형태로 사용함에도 낮은 어류내 생체내이용율을 가진다는 사실을 알 수 있다. The pharmacokinetic properties of amoxicillin trihydrate (Amox) were studied after single oral administration and single intravenous injection to cultured eel, Anguilla japonica, respectively (average 220±10 g, 28±1℃). Plasma samples were taken at 3, 5, 10, 15, 24, 30, 48, 96 and 144 h post-dose. The kinetic profile of absorption, distribution and elimination of Amox in plasma were analyzed fitting to a two-compartment model by WinNonlin program. In oral dosage of 40 and 80 ㎎/㎏ body weight, the peak plasma concentrations of Amox, which attained at 3∼12 h post-dose, were 3.4 and 3.3 ㎍/㎖, respectively. In intravenous injection with 1 ㎎/㎏, the peak plasma concentrations of Amox, which attained at 9 h post-dose, was 7.2 ㎍/㎖. The following parmeters were calculated for a single oral dosage of 40 and 80 ㎎/㎏ body weight, respectively: AUC (the area under the concentration-time curve)〓 464 and 667 ㎍·h/㎖; Tmax (time for maximum concentration)〓 2.1 and 3.6 h; Cmax (maximum concentration)〓 3.04 and 3.4 ㎍/㎖. Following intravenous injection at 1 ㎎/㎏, this parameters were AUC= 748 ㎍·h/㎖; Cmax〓 4.2 ㎍/㎖. The apparent oral bioavailability at 40 and 80 ㎎/㎏ were 1.6 and 1.1%, respectively. Despite using the trihydrate form of amoxicillin, the oral bioavailability was low in eel.

        • KCI등재

          일반 논문 : 중소기업의 체계적 현장직무교육훈련(S-OJT) 프로그램 특성이 조직몰입과 이직의도에 미치는 영향

          전은지 ( Eun Ji Jeon ),송영선 ( Young Sun Song ),이희수 ( Hee Su Lee ) 한국중소기업학회 2011 中小企業硏究 Vol.33 No.4

          본 연구의 목적은 학문적으로는 S-OJT를 통한 중소기업 인력의 조직몰입과 이직의도에 대한 연구기반을 제공하고 실무적으로는 중소기업이 인력을 효과적으로 유지할 수 있는 현장 지향적 정책 제언을 제시하는데 있다. 이 목적을 위해 고용노동부가 주관하고 한국산업인력공단에서 시행하는 ``체계적 현장직무교육훈련(S-OJT)`` 시범사업에 참여한 중소기업의 S-OJT 프로그램 특성이 조직몰입과 이직의도에 미치는 영향과 조직몰입이 이직의도에 미치는 영향을 분석하였다. 연구대상은 ``체계적 현장직무교육훈련(S-OJT)`` 시범사업에 참여한 중소기업 중 부산·경남지역의 중소기업 종업원 151명을 대상으로 하였다. 연구방법은 구조화된 설문지를 활용하여 자료를 수집하고 분석은 SPSS 19.0 통계 패키지를 이용하여 요인분석, 상관관계분석, 다중회귀분석 등을 사용하였다. 이를 통해 S-OJT 프로그램 특성요인, 조직몰입, 이직의도와의 관계를 분석하였다. 연구결과는 다음과 같다. 첫째, S-OJT 프로그램 특성요인이 중소기업 종업원의 조직몰입에 영향을 미치는 것으로 나타났다. 조직몰입 중 감정적 몰입에 유의한 영향을 미치는 S-OJT 프로그램 특성요인은 교육훈련환경, 체계적 시스템 구축, S-OJT 지원이다. 조직몰입 중 지속적 몰입에서는 S-OJT 지원이 영향을 미치는 요인으로 밝혀졌다. 조직몰입 중 규범적 몰입에서는 S-OJT 프로그램 특성요인 중 체계적 시스템 구축과 S-OJT 지원이 유의미한 영향을 미치는 것으로 나타났다. 둘째, S-OJT 프로그램 특성요인 중 교육훈련환경이 이직의도에 유의한 부적 영향을 미치는 것으로 나타났다. 셋째, S-OJT 프로그램을 실시하는 중소기업 종업원의 조직몰입이 이직의도에 부분적으로 영향을 미치는 것으로 나타났다. 이러한 연구 결과가 주는 시사점을 정리하면, 첫째 학문적으로는 중소기업에 맞는 S-OJT 프로그램의 특성을 제시하고 실증적으로 검증하였다는 점과 둘째, 실제적으로는 정부 및 중소기업 교육담당자들에게 S-OJT에 대한 지속적인 관심과 투자를 유지하고 확대할 수 있는 객관적인 근거와 정책 제언을 제시하였다는 점이다. The aim of this study is to provide basic data for development of domestic medium and small enterprises [hereinafter, MSE] by offering a research foundation on organizational commitment and turnover of the human resources for MSE academically, and suggesting a practical management plan and work based policy that will make it possible for domestic MSE to effectively retain their human resources by a structured on-the-job training [hereinafter, S-OJT] program. For this purpose, this study analyzed the influence of the S-OJT program characteristics of a MSE, which participated in the pilot project implemented by the Human Resources Development Service of Korea and was supervised by the Ministry of Employment and Labor, on organizational commitment and turnover intention, and also analyzed the influence of organizational commitment on turnover intention. This study surveyed 151 employees in MSE in the Busan and Gyungnam district among MSE which participated in a S-OJT pilot project as research subjects. As for the research method, this study collected data using structured questionnaires and for the analysis method, this study implemented a factor analysis, correlation analysis, and multi-regression analysis using SPSS ver. 19.0. Validity and reliability was verified the following; To verify validity, this study composed a survey based on the selected questions from a preliminary study. From the results of exploratory factor analysis on question variable at preliminary study, the eigen value of education and training environment as a sub-variable of S-OJT was 3.509 and the eigen variance explanatory power thereof was 70.177. Ability of trainer eigen value was 3.506 and eigen variance explanatory power thereof was 58.440. The eigen value of building a systematic system was 3.525 and the eigen variance explanatory power thereof was 70.491. The S-OJT program support eigen value was 5.397 and the eigen variance explanatory power thereof was 67.462. The turnover intention eigen value was 3.104 and the eigen variance explanatory power thereof was 62.089. Five questions were all tied by one factor. Organizational commitment had 24 questions that were grouped by factors of affective commitment, continuance commitment and normative commitment. The eigen value was 1.793~5.972, and the eigen variance explanatory power thereof appeared at 11.205~37.326. To confirm the validity of subordinate factors extracted from the preliminary study, factor analysis was implemented. Construct validity of the measuring tool for variables used in the preliminary study was evaluated. Factor analysis used PCA (Principal Component Analysis), and for factor rotation method varimax, which is a right angle rotation method, was used. Determination of the number of factors among variables was done by selecting factors with eigen value of not less than 1. Also, for communality, the questions were excluded if the sum of all factor load square values were 0.4 or less. The guideline used was loadage value of this factor not less than 0.5 and variance explanatory power of not less than 0.5 (50%). To verify reliability, Cronbach`s alpha as a good reliability verification method measuring internal consistency within measured items was used. This method enhances reliability of measurement devices by hunting the factor out of multiple questions that degrades the confidence of the measuring device and excluding the question. Characteristics of S-OJT Programs showed a high reliability with total Cronbach`s alpha value at .964. By each subordinate variable, Cronbach`s alpha ranged .840~.901 at a reliable level. Organizational commitment showed significantly high reliability with total Cronbach`s alpha value at .906. By each subordinate variable, Cronbach`s alpha ranged .718~.834 at a significantly reliable level. Turnover intention total Cronbach`s alpha was .780 at a reliable level. Through this process, this study analyzed the relationship between S-OJT program characteristics, organizational commitment, and turnover intention. The study results are as follows : First, this study verified the direct effect that the characteristic factors in S-OJT program had on the leading variables of organizational commitment such as continuance commitment, affective commitment and normative commitment in MSE. The verification result shows that the characteristic factors in S-OJT program increase commitment among organizational participants. This result allows an expectation of higher organizational commitment when a S-OJT Program is implemented. Especially, the characteristic factor of an S-OJT Program has the strongest effects on affective commitment. In other words, the characteristic factor of an S-OJT Program is the meaningfully predicting factor explaining affective commitment among organizational commitment. From this result, S-OJT is indirectly proven as superior to unsystematic OJT in lower defect ratio, higher productivity, and more efficiency in training. The belief that a S-OJT Program may replace existing OJT Program or solve problems thereof may be regarded as confirmed by this result. Second, the study found that the higher the average of characteristic factor in S-OJT program, the lower the turnover intention of the employees involved in MSE. Among them, education and training environment as a characteristic factor in an S-OJT Program appeared as giving a meaningfully negative influence to turnover intention of SME employees. This result verifies that domestic SMEs need hardware such as educational and training environment, system construction, S-OJT support etc., ahead of software such as the ability of the trainer, for implementation of S-OJT for the purpose of keeping down turnover intention. Therefore, the implication is that educational and training environment setting for continuous human resource development is important to keep down turnover intention as one measure to enhance SME`s survival and competitiveness. Third, higher affective commitment of employees implementing S-OJT Program showed lower turnover intention. However, continuance commitment and normative commitment did not appear as having any meaningful influence on turnover intention. This result shows that affective commitment of SME employees such as esprit de corps, will to work, receptivity etc. is a directly affecting factor on turnover intention rather than other commitments. To enhance commitment by SME employees, a group solidarity function enhancing program is required to raise group solidarity and unity. Also, to prevent weakening of organizational commitment, a program should be developed to strengthen the proper function of organizational commitment. The program to strengthen proper function of organizational commitment is suggested as an activation of communication between organizational participants and leisure programs. Therefore, government officials and SME staff in charge of SME. S-OJT shall provide various programs to enhance affective commitment when implementing S-OJT. This study makes some political suggestions in order for S-OJT of MSE sites to revitalize S-OJT and make actual performance happen as follows: First, there is a need for promotional strategies at a national level for executives and employees to have a paradigm shift in S-OJT. Generally, managers have a tendency to be indifferent or even to ignore education and training due to the matter of time and cost. Accordingly, the nation and government are required to seek successful cases and promotional strategies in an effort to implant the fact that the S-OJT system is a suitable one for progress in productivity, organizational commitment, mutual communication between staff members, and smooth relations between labor and management, etc. into the business owners [managers]of MSE. Second, in order for S-OJT to be continuously sped up, it`s necessary to operate the education and training programs such as S-OJT executive course, on-site administrator course, and on-site leader course, etc. so that a manager, on-site supervisor and hands-on staff can understand and practice the S-OJT. The programs for education and training are required to concretely set up the matters such as drawing up of a training plan, trainers in charge, trainees, dates for training and training contents, etc. in order to systematically and effectively practice the S-OJT continuously. Third, in an effort to foster the S-OJT atmosphere, it is advisable to link the outcome of implementation of the S-OJT to the corporate personnel management system such as employment, a personnel reshuffle, salary, promotion and retirement, etc. However, principally, personnel management should be on the basis of fairness and objectivity, so it requires concrete behavioral standards, a criterion of its implementation, and objective observation and evaluation at the time of implementing a S-OJT. This study has a limit to the generalization of these research results because this study was set among employees for MSE involved in the manufacturing business in Busan and Gyungnam district among the enterprises participating in a pilot project for ``Structured S-OJT``, which was conducted in the second half of the 2010, as its research target. In addition, there still remains a sense of frustration for not making a qualitative [qualitative research] assessment through the interviews with an executive, education and training practitioner in charge and employees other than settling for a quantitative assessment [quantitative research] through the analysis and description using literature review, and objective statistical treatment. Nevertheless, this study can be fairly said to be full of suggestions in that this study first suggested and empirically verified the properties of S-OJT programs suited for MSE from a scientific standpoint; second, this study suggested objective grounds for expansion of investment in an effort for continuous concern and improvement in S-OJT to the government and educational practitioners in MSE from a practical standpoint; third, this study suggested a political suggestion about how to revitalize the S-OJT more and to produce a great outcome for the government and educational practitioners.

        • KCI등재

          수산양식에서 친환경성 약제개발을 위한 항병성 생약소재의 검토

          전은지(Eun-Ji JEON),이남실(Nam-Sil LEE),서정수(Jung-Soo SEO),정승희(Sung-Hee JUNG),김명석(Myung-Sug KIM),강소영(So-Young KANG),김나영(Na-Young KIM) 한국수산해양교육학회 2020 水産海洋敎育硏究 Vol.32 No.4

          This study is examination about herb medicines that counters the effects of a fish pathogens, and is for eco-pharmaceutical development in aquaculture industry. 13 kinds of herb medicines were selected, and the hot water extracts and ethanol extracts were tested for antibacterial, antiparasitic, antifungal and antiviral effects. Minimal inhibitory concentration (MIC) of 13 kinds of candidate herb medicines were measured, above all, hot water and ethanol extracts of In-jin (1/10 and 1/10² dilution) showed antibacterial activities against 14 kinds of fish pathogenic bacteria. Hot water extract and ethanol extract of Dang-gwi showed antiparasitic and antifungal activities, and the extract had strong antifungal activity especially (~1/10³ dilution). In antiviral activity against VHSV (Viral hemorrhagic septicemia virus), hot-water and ethanol extract of In-jin and Dang-gwi were effective down to 1/10³ dilution. From these results, In-jin and Dang-gwi were selected as effective herbs among 13 kinds of herb medicines and further study on the synergy effect using combination with in-jin and dang-gwi against fish pathogens will be applied to fish immune response.

        • KCI등재후보

          양식산 넙치에서 Enrofloxacin의 잔류

          서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),이은혜 ( Eun Hye Lee ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),지보영 ( Bo Young Jee ),김나영 ( Na Young Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.1

          Enrofloxacin is one of the normally used flouroquinolones in mammalian and fish but its withdrawal time and studies were remain obscure. The residual contents of enrofloxacin in fish muscle were analyzed by using HPLC-FLD. More than 0.1 mg/kg of ENR was detected in muscle tissues and the residues were found over 1 year after treatment. The concentration of ENR in Paralichthys olivaceus was not affected by water temperature and lasted for an extended amount of time. The spike recoveries of ENR in the muscle tissue ranged from 78% to 85%. From this results, we need the prescription by veterinarian or aquatic organism disease inspector in ENR usage to assure safety of fish. Future research is required to determine the recommendation dose of ENR for side effects and safety.

        • KCI등재후보

          단보 : 유카추출물과 didecyldimethylammonium chloride를 주성분으로 하는 살균소독제 아쿠아 팜세이프의 어류병원체에 대한 살균 효과

          서정수 ( Jung Soo Seo ),전은지 ( Eun Ji Jeon ),황지연 ( Jee Youn Hwang ),정승희 ( Sung Hee Jung ),박명애 ( Myoung Ae Park ),이성민 ( Sung Min Lee ),이은혜 ( Eun Hye Lee ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2

          본 연구에서는 4급 암모늄에 속하는 didecyldimethylammonium chloride (DDAC)와 유카추출물 (Yucca extract)의 합제인 아쿠아 팜세이프®의 어류 질병 병원체에 대한 살균효과를 확인하기 위하여 농림수산검역 검사본부의 소독제 효력시험 중 세균 및 바이러스 소독제 효력시험에 따라 수행하였다. 소독제와 병원체를 증류수, 경수 그리고 유기물 조건에서 반응시킨 후, 중화액을 이용하여 중화시킨 다음, 중화된 용액을 배지 및 주화세포에서 접종시켜 세균의 성장 및 세포 변성효과를 통해 소독제의 효력배수를 결정하였다. 본 연구의 결과, 아쿠아 팜세이프®는 식중독을 일으키는 공시균주 살모넬라균 (S. typhimurim)에 비하여, 어병세균이 30~40배 이상 높은 희석농도에서 소독 효과를 나타내었으며, VHSV에 대하여서는 2,000~3,600배의 유효한 효력배수를 나타내었다. 따라서, 향후, 야외적용시험을 통해 실제 적용에 따른 효과를 확인할 필요가 있을 것으로 사료된다. In this study, the disinfectant efficacy of Aqua farmsafe®, composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe® possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe® against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua framsafe® will be able to reduce fish diseases.

        • KCI등재

          복합 생약재 열수추출물 투여에 따른 넙치 세균성 질병에 대한 질병저항성

          서정수(Jung Soo SEO),전은지(Eun Ji JEON),권문경(Mun Gyeong KWON),황지연(Jee Youn HWANG),정승희(Sung Hee JUNG),김나영(Na Young KIM),지보영(Bo Young JEE),박명애(Myoung Ae PARK) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.6

          복합 생약재 열수추출물(6종 혼합)을 농도별 기간별로 사료에 흡습시켜 12주 동안 넙치에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 복합 생약재 열수추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았으며 혈액생화학적인 , 결과도 넙치의 생리에는 아무런 영향을 미치지 않았다. 라이소자임 활성은 0.01%의 복합 생약재 열수추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 복합 생약재 열수추출물의 투여에 따른 넙치 장기의 병리조직에 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병 저항성은 0.01% 복합 생약재 열수추출물 농도에서 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 넙치에 복합 생약재 열수추출물을 투여시에는 어류의 비특이적 면역력 증강 및 질병 저항성 증강을 나타내는 것으로 사료되었다. The heat extracts of six kinds of medicinal herbs (Scutellaria baicalensis, Sophora flavescens, Citrus unshiu pericarpium, Lonicera japonica, Perilla frutescens, Benincasa hispida) were tested for non-specific immune response and disease resistance effects related with fish diseases on olive flounder, Paralichthys olivaceus. The preventive effects of 6 herbal mixtures against bacterial disease on cultured flounder were examined follow as feeding EP absorbed with the heat extract of six kinds of medicinal herbs. For feeding trial for 12th week, weight gain and serum analysis of fish fed various groups were not significant differences. Lysozyme activity of the 0.01 % treated group on 4th week showed significant increase. Histopathology of the administrated group for feeding period showed no particular signs of tissue degeneration. At 0.01% oral experimental group, relative percent survival (RPS) were only 50% to 75% for four weeks and eight weeks group by intraperitoneal injection with E. tarda. The results suggest that heat extracts of six kinds of medicinal herbs (0.01%) would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish pathogen.

        • KCI등재

          생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향

          서정수(Jung-Soo SEO),전은지(Eun-Ji JEON),권문경(Mun-Gyeong KWON),황지연(Jee-Youn HWANG),김진도(Jin-Do KIM),정승희(Sung-Hee JUNG),김나영(Na-Young KIM),지보영(Bo-Young JEE),박명애(Myoung-Ae PARK) 한국수산해양교육학회 2015 水産海洋敎育硏究 Vol.27 No.6

          고삼 뿌리 열수 추출물을 농도별로 넙치 사료에 흡습시켜 12주 동안 어류에 급여 후 비특이적 면역 및 질병저항성에 대한 효과를 조사하였다. 고삼 추출물의 첨가는 어류 성장에는 아무런 영향을 미치지 않았다. 혈액화학적인 조사 결과에서도 고삼 첨가로 인한 넙치의 생리에는 아무런 영향을 나타내지 않았다. 라이소자임 활성은 0.05%의 고삼 추출물 농도로 흡습시킨 사료 그룹에서 라이소자임 활성이 증가됨을 알 수 있었다. 백혈구의 식세포 활성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서 유의적으로 증가되었다. 병리학적 결과를 통하여 고삼 추출물을 농도별로 넙치에 투여하였을때 병리조직학적 영향이 없음을 알 수 있었다. 에드와드균의 인위감염에 의한 질병저항성은 0.05%의 고삼 추출물로 흡습시킨 사료 그룹에서만 상대생존율이 높게 나타났다. 이상의 결과를 종합하여 볼 때 고삼뿌리 열수 추출물은 넙치에 대한 비특이적 면역력 증강 및 질병 저항성 증강에 효과가 있는 것으로 사료되었다. The effects of lightyellow sophora, Sophora flavescens extract were tested on non-specific immune response and a disease resistance of olive flounder, Paralichthys olivaceus. For feeding trial, weight gain of fish fed different treated groups were not significant differences. Serum analysis of treated and control group did not demonstrated any differences. Lysozyme activity in the kidney and spleen of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase, respectively. In addition, phagocytic activity of the 0.05% treated group on 4<SUP>th</SUP> and 8<SUP>th</SUP> week showed significant increase compared to the control group. Histopathology of the liver and kidney tissue of the administrated group for 4<SUP>th</SUP>, 8<SUP>th</SUP> and 12<SUP>th</SUP> week showed no particular signs of tissue degeneration. The treated group was higher than control group by analyzing the relative percent survival (RPS) of the experimental challenge of Edwardsiella tarda. Therefore, the lightyellow sophora showed effective in preventing fish-pathogenic edwardsiellosis for a certain period of time.

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