잔류 항균물질에 대한 미생물학적 간이검사법의 검출감도 비교

정승희,김진우,손상규,JUNG Sung Hee,KIM Jin Woo,SOHN Sang-Gyu 한국수산과학회 1999 한국수산과학회지 Vol.32 No.3

Minimun-detectable levels to 28 antibacterial agents used for the prevention and the treatment of fish diseases were determined to establish optimal detective method of bioassay in fish by the EEC 4-plate method, the modified method of EEC 4-plate and the standard method of analysis in food safety regulation. The test organisms used in the methods of bioassay were as follows: Bacillus subtilis BGA (B. subtilis) and Micrococcus luteus ATCC 9341 (M. luteus) in the EEC 4-plate method, B. subtilis, M. luteus and Bacillus cereus var. mycoides ATCC 11778 (B. cereus) in the modified of EEC 4-plate, and B. subtilis, M. luteus, B. cereus and Bacillus stearothermophilis var. calidolactis C-953 (B. stearothermophilis) in the standard method. The standard method showed predominant sensitivity in the detection of penicillins (PCs), and was also highly sensitive to aminoglycosides (AGs). The sensitivity of standard method in the detection of tetracyclines (TCs), marrolides (MLs), nitrofuran derivatives(NFs) and quinolones (QNs) was very low, and against sulfonamides (SAs), however, was extremely low. The modified method of EEC 4-plate showed very high sensitivity to TCs. Both the EEC 4-plate and the modified method of EEC 4-plate showed competitively high sensitivity in the detection of PCs, MLs, NFs, QNs and SAs. All the methods studied in the experiment showed very low sensitivity against chloramphenicol (CMs). Consequently, the modified method of EEC 4-Plate was the best bioassay method with a wide range of sensitivity for the optimal detection of the residual antibacterial agents in fish.

넙치, Paralichthys olivaceus에 nalidixic acid, piromidic acid, oxolinic acid의 경구투여 약물동태에 미치는 수온의 영향

정승희 ( Sung Hee Jung ),김진우 ( Jin Woo Kim ),서정수 ( Jung Soo Seo ),최동림 ( Dong Lim Choi ),지보영 ( Bo Young Jee ),박명애 ( Myoung Ae Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.1

Effects of temperature (13±1.5℃, 23±1.5℃) on the pharmacokinetic properties of nalidixic acid (NA), piromidic acid (PA) and oxolinic acid (OA) were studied after oral administration to cultured olive flounder, Paralichthys olivaceus. Serum concentrations of these antimicrobials were determined after oral administration of a single dosage of 60㎎/㎏body weight (average 700g). At 23±1.5℃, the peak serum concentrations of NA, PA and OA, which attained at 10 h, 24 h and 30 h post-dose, were 11.55, 3.79 and 1.12㎍/㎖, respectively. At 13±1.5℃, the peak serum concentrations of NA, PA and OA, which attained at 10 h, 15 h and 30 h post-dose, were 6.36, 1.4 and 1.01㎍/㎖, respectively. Better absorption of NA and PA was noted at 23±1.5℃ compared to 13±1.5℃. The elimination of NA from serum of olive flounder was considerably faster at 23±1.5℃ than at 13±1.5℃. However, both absorption and elimination of OA were not affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of these antimicrobials in serum were analyzed by fitting to a one-and two compartment model, with WinNonlin program. In the one compartment model for NA, AUC, Tmax and Cmax at 23±1.5℃ were 258.26㎍·h/㎖, 10.67 h and 8.91㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 341.45㎍·h/㎖, 7.72 h and 6.23㎍/ ㎖, respectively. In the one compartment model for PA, AUC, Tmax and Cmax at 23±1.5℃ were 248.12㎍·h/㎖, 21.15 h and 3.09㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 103.89㎍·h/ ㎖, 12.89 h and 1.22㎍/㎖, respectively. In the two compartment model for OA, AUC, Tmax and Cmax at 23±1.5℃ were 138.20㎍·h/㎖, 23.95 h and 1.06㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 159.10㎍·h/㎖, 28.03 h and 1.02㎍/㎖, respectively.

Ampicillin의 경구, 주사 및 약욕 투약에 따른 넙치 혈액에서의 잔류량 변화

정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),박명애 ( Myoung Ae Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3

The residue levels of ampicillin (AM) in cultured olive flounder, Paralichthys olivaceus (average 300g) at 20±1.0℃ were studied by oral, intramuscular and dipping administration (routes). The concentrations of AM in the plasma were determined by HPLC-UV detector. The average recoveries of AM in spiked samples between 0.01∼10 ppm were ranging from 84.45% to 91.26% for plasma. The limit of detection for AM was 0.05 ppm by using this method. Plasma concentrations of AM were determined after oral dosage (10, 20 and 40 ㎎/㎏body weight), intramuscular injection (5, 10 and 20 ㎎/㎏body weight) and dipping (10, 20 and 40 ppm; 1 h). Samples were taken at 1, 3, 5, 10, 15, 24, 30, 48, 96, 144, 216, 264 and 360 h post-administration. In oral dosage of 10, 20 and 40 ㎎/㎏, it`s peak concentrations were 3.62±0.97, 5.20±0.70 and 11.18±0.87 ㎍/㎖, respectively at 10 h post-administration, but AM was not measurable at 144, 360 and 360 h post-administration, respectively. In intramuscular injection of 5, 10 and 20 ㎎/㎏, it`s peak concentrations were 6.92±1.29 ㎍/㎖, 9.89±2.22 ㎍/㎖and 19.85±2.97 ㎍/㎖, respectively at 5 h post-administration, but AM was not measurable at 216, 264 and 264 h post-administration, respectively. In dipping of 10, 20 and 40 ppm, it`s peak concentrations were 4.39±1.10, 9.57±1.51 and 11.61±1.92 ㎍/㎖, respectively at 3 h post-administration, but AM was not measurable at 264, 264 and 360 h post-administration, respectively. Therefore, the plasma distribution and elimination levels of AM in olive flounder were dosage-dependant manner in all administration routes.

미생물학적 방법에 의한 어체내 잔류 항균물질의 계열별 동정시험

정승희(Sung Hee Jung),김진우(Jin Woo Kim) 한국어병학회 1997 한국어병학회지 Vol.10 No.2

The possibility of identification of families of antibacterial agent residues in fish tissue was studied by disk assay using three test organisms, Bacillus subtilis BGA, Micrococcus luteus ATCC 9341, and Bacillus cereus var. mycoides ATCC 11778. In the present method, a simple clean-up procedure was performed to obtain the aqueous solution from homogenized flounder muscle sample(10g) in Mcilvaine buffer. Then, aqueous solution was fractionated into A and B to be used in disk assay by choloroform and Sep-Pak C_(18) cartridge column after being defatted in hexane. The chloroform layer of fraction A was used for the analysis of macrolide antibiotics(ML), sulfa drugs(SA), chloramphenicol(CP), and quinolone antibiotics(QN). Adsorbed materials to Sep-Pak C_(18) of fraction B were also employed for the analysis of penicillins(PC), tetracyclines(TC), and nitrofuran derivatives(NF) Minimun-detectable concentrations by the present method were, 0.1㎍/g for oxytetracycline, tetracycline, doxycycline, spiramycin and ciprofloxacin, 0.025㎍/g for erythromycin and ampicillin, 1.0㎍/g for sodium nifurstyrenate and florfenical, 0.25㎍/g for sulfamonomethoxi e and sulfadimethoxine, 2.5㎍/g for oxolinic acid and flumequine, and 15㎍/g for piromidic acid, respectively. Three test organisms showed different sensitivity patterns for each family of antibacterial agent. Sensitivity patterns were B. cereus > B. subtilis > M. luteus for TC and NF, M. luteus > B, subtilis > B. cereus for ML and PC, B. cereus = B. subtilis > M. luteus for CP and QN, and B. subtilis > B. cereus=M. luteus for SA. The present method utilizing these characteristics could be useful as a routine screening test for the determination of family of antibacterial agent residues in fish tissue.

Oxolinic acid의 경구투여, 주사 및 약욕에 따른 넙치, Paralichthys olivaceus 체내 약물동태학적 특성

정승희 ( Sung Hee Jung ),최동림 ( Dong Lim Choi ),김진우 ( Jin Woo Kim ),조미라 ( Mi Ra Jo ),지보영 ( Bo Young Jee ),서정수 ( Jung Soo Seo ) 한국어병학회 2009 한국어병학회지 Vol.22 No.2

단보 : Oxytetracycline을 근육 주사한 넙치(Paralichthys olivaceus)의 약물동태학적 특성

정승희 ( Sung Hee Jung ),최동림 ( Dong Lim Choi ),김진우 ( Jin Woo Kim ),조미라 ( Mi Ra Jo ),서정수 ( Jung Soo Seo ),지보영 ( Bo Young Jee ) 한국어병학회 2009 한국어병학회지 Vol.22 No.1

뱀장어에 대한 포르말린의 24시간 반수치사농도(LC50)

정승희 ( Sung Hee Jung ),이주석 ( Joo Seok Lee ),지보영 ( Bo Young Jee ),서정수 ( Jung Soo Seo ),김진우 ( Jin Woo Kim ),김응오 ( Eung Oh Kim ) 한국어병학회 2007 한국어병학회지 Vol.20 No.2

Thiamphenicol의 단일 경구투여에 따른 넙치와 조피볼락의 혈액내 잔류 분석

정승희(Sung-Hee JUNG),서정수(Jung-Soo SEO) 한국수산해양교육학회 2016 수산해양교육연구 Vol.28 No.3

This study was aimed at investigating the residual patterns of thiamphenicol (TP) in the cultured olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes schlegeli) following oral administration. TP concentration were detected by high performance liquid chromatography with UV detector. The recovery rates of TP in serums ranged 77.05~97.23% (olive flounder) and 89.96~97.11% (black rockfish) for the concentration of 0.1, 1.0, 10 ㎍/mL, respectively. After single administration of TP (100 ㎎/㎏ body weight) by oral route in olive flounder (700±50 g, 23±1.5℃) and black rockfish (500±30 g, 23±1.5℃), the concentration in the serum was determined at 1, 5, 10, 15, 24, 30, 50, 168, 264 and 432 h post-dose. Two-compartment model was applied to analyze in the pharmacokinetics of TP administered to the fishes. In the serum of olive flounder, TP was detected on 10 and 15 hours after treatment were 10.08 ㎍/mL and 10.06 ㎍/mL as maximum level, respectively. In the serum of black rockfish, TP was detected on 15 hours after treatment were 8.88 ㎍/mL as maximum level. Concentrations of TP to the fishes were not measurable at 432 hours (18 days) after treatment in all samples. Similar residual patterns of TP were revealed between the fishes. These results are helpful for estimating withdrawal time of TP which has been already in use for farmed fish treatment.

조피볼락, Sebastes schlegeli에 경구투여된 oxolinic acid의 약물동태에 미치는 수온의 영향

정승희 ( Sung Hee Jung ),김진우 ( Jin Woo Kim ),서정수 ( Jung Soo Seo ),지보영 ( Bo Young Jee ),박명애 ( Myoung Ae Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.2

Effects of temperature (13±1.5℃, 23±1.5℃) on the pharmacokinetic properties of oxolinic acid (OA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of OA were determined using HPLC-UV detector after a single dosage of 60 ㎎/㎏ body weight (average about 500 g). The peak serum concentrations of OA at 23±1.5℃ and 13±1.5℃ were 0.60 ㎍/㎖ at 30 h and 2.22 ㎍/㎖ at 10 h post-dose, respectively. Better absorption of OA was noted at 13±1.5℃ compared to 23±1.5℃. The elimination of OA from serum was considerably faster at 23±1.5℃ than at 13±1.5℃. Both absorption and elimination of OA were affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of OA in serum was analyzed by fitting to a two compartment model, with WinNonlin program. The AUC, Tmax and Cmax at 23±1.5℃ were 42.16 ㎍?h/㎖, 26.13 h and 0.43 ㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 131.98 ㎍?h/㎖, 8.81 h and 2.04 ㎍/㎖, respectively.

포르말린 약욕이 뱀장어 아가미흡충의 구제에 미치는 효과

정승희 ( Sung Hee Jung ),지보영 ( Bo Young Jee ),김진도 ( Jin Do Kim ),서정수 ( Jung Soo Seo ),김진우 ( Jin Woo Kim ) 한국어병학회 2010 한국어병학회지 Vol.23 No.3

Effects of formalin on removal of Pseudodactylogyrus spp. were examined against naturally infected eel, Anguilla japonica (weight 89.9∼96g) at a water temperature of 28℃. Prior to experiments for removal of the parasite, the hematological toxicity of formalin bath at 0∼500 ppm for 30 min∼24 h was assessed by hematocrit values (Ht). Based on the results of Ht, appropriate methods of treatment, concentrations and durations, were examined in the main study. There was no significant (P>0.05) change of Ht in 100 to 200 ppm for 24 h. In contrast, Ht increased significantly (P<0.05) at above 300 ppm. This suggests that physiological damage was caused by formalin bath treating with 300 to 500 ppm. Formalin bath with 100 and 200 ppm for 24 h caused significant decreases (P<0.05) in the infection of the parasite. In conclusion, the 100 ppm formalin for 24 hour-bath was found most recommendable for the effective treatment of Pseudodactylogyrus spp. for the gills of the infected eel because of the median lethal concentration (LC50) of formalin to eel; cumulative mortalities were found to be 0 and 13.3%, respectively, following 24 h bathing.