담수산 양식어류의 혈액검사에 사용된 건식 자동생화학 분석기 (FUJIDRI-CHEM 3000) 의 활용 가능성

정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),김진도 ( Jin Do Kim ),최혜승 ( Hye Sung Choi ),박명애 ( Myoung Ae Park ) 한국어병학회 2011 한국어병학회지 Vol.24 No.3

The purpose of this study was to obtain reference data of parameters for hematological health diagnosis in cultured freshwater fish and also evaluate application of automatic dry-type chemistry analyzer (FUJI DRI-CHEM 3000) used to those blood tests. A blood profile of total 200 fish for rainbow trout (Onchorhynchus mykiss), israel carp (Cyprinus carpio), tilapia (Oreochromis niloticus) and eel (Anguilla japonica) cultured in Inland Fisheries Research Institute of NFRDI was determined by hemoglobin (Hb) and plasma chemistry tests: total protein (TP), albumin (ALB), alkaline phosphatase (ALP), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), triglyceride (TG), total cholesterol (TCHO), creatinine (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose (GLU). The values of ALT, TG, LDH, ALB, TCHO, AST and ALP were outside from the minimum and/or maximum of the established detectable range of the analyzer. ALT and TG were not detectable in the range of 67%∼61.5%. LDH, ALB and TCHO were not detectable in the range of 36∼17%. AST and ALP were not detectable in the range of 5.5∼0.5%. However, the values of BUN, CRE, GLU, Hb and TP were below the detectable limits of the analyzer.

해상가두리 양식장 어류의 혈액 검사에 사용된 동물용 생화학 분석장치의 활용 가능성

정승희 ( Sung Hee Jung ),변순규 ( Soon Gyu Byun ),지보영 ( Bo Young Jee ),최혜승 ( Hye Sung Choi ) 한국어병학회 2006 한국어병학회지 Vol.19 No.3

사육방법 및 크기에 따른 조피볼락의 혈청 화학성분 변화

정승희(Sung-Hee JUNG),서정수(Jung-Soo SEO),최혜승(Hye-Sung CHOI) 한국수산해양교육학회 2014 水産海洋敎育硏究 Vol.26 No.4

조피볼락 친어 3개 그룹(서해자연산, 서해가두리양식산, 동해자연산)과 치어 5개 그룹(서해자연산, 서해육산양식산, 서해육상·가두리양식산, 서해축제식·가두리양식산, 서해축제식양식산)에 대하여 사육방법과 크기의 차이에 따라서 9개의 혈청화학성분이 어떠한 변화를 나타내는지 알아보고자 하였다. 그 결과, 7개 항목인 총단백, 알부민, 총콜레스테롤, AST, ALT, LDH, ALP의 함량에서 친어 및 치어그룹 간에 유의적인 차이가 관찰되었다. 즉, 총단백, 알부민, 총콜레스테롤, ALP는 친어그룹이 치어그룹에 비해 높은 농도를 보였으며, 반대로 AST, ALT, LDH는 치어그룹이 친어그룹에 비하여 높은 농도를 나타내었다. 이러한 결과는 사육방법과 크기(친어, 치어)가 서로 다른 조피볼락의 생리적인 변화와 관련이 있는 것으로 판단된다. The purpose of this study was to obtain reference values of serum chemistry in Korean rockfish (Sebastes schlegeli), 5 juvenile (n=25) and 3 broodstock (n=15) subpopulations which were differentiated by cultured method. Serum samples were analyzed for the following commercial clinical kits: total protein, albumin, glucose, AST (aspartate aminotransferase), ALT (alanine aminotransferase), total cholesterol, free cholesterol, LDH (lactate dehydrogenase) and ALP (alkaline phosphatase). Significant differences were observed in the values of total protein, albumin, total cholesterol, AST, ALT, LDH and ALP between juvenile and broodstock subpopulations. In the comparison of total protein, albumin, total cholesterol and ALP, broodstock subpopulations showed higher levels. On the other hand, increased AST, ALT and LDH were observed in juvenile subpopulations. These results indicate that cultured method are major contributors to health-related physiology of Korean rockfish, juveniles and broodstocks.

2007년∼2011년 하절기에 양식 넙치, 조피볼락, 참돔, 새우의 세균 및 기생충 감염 현황

정승희 ( Sung Hee Jung ),최혜승 ( Hye Sung Choi ),도정완 ( Jeung Wan Do ),김명석 ( Myoung Sug Kim ),권문경 ( Mun Gyeong Kwon ),서정수 ( Jung Soo Seo ),황지연 ( Jee Youn Hwang ),김석렬 ( Seok Ryel Kim ),조영록 ( Yeong Rok Cho ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3

2007년∼2011년 하절기에 국내 동·서·남해안과 제주의 육상양식장, 가두리 및 축제식 양식장에서 사육중인 넙치 (Paralichthys olivaceus), 조피볼락(Sebastes schlegeli), 참돔 (Pagrus major), 흰다리새우(Litopenaeus vannamei) 의 총 2,413마리에 대하여 세균과 기생충의 감염현황을 조사하였다. 조사기간(2007년∼2011년) 사이 어종별 병원체에 대한 감염양상을 종합하면, 기생충과 세균의 단독감염이 혼합감염에 비하여 비교적 높게 나타났으며, 단독감염 가운데 기생충이 세균보다 감염률이 높은 경향을 나타내었다. 조사기간 동안 넙치, 조피볼락, 새우에서 Vibrio spp. (V. harveyi, V. ichthyoenteri, Vibrio sp.)가 가장 우점종으로 검출된 세균의 위치를 차지하였으며, 참돔에서는 Vibrio sp.와 P. damselae가 우점종 세균으로 검출되었다. 조사기간 동안 가장 우점종으로 검출된 기생충은 넙치에서 스쿠티카충 (M. avidus)과 트리코디나충 (Trichodina sp.), 조피볼락에서 아가미흡충 (M. sebastes), 참돔에서 아가미흡충 (M. tai), 새우에서 섬모충 (Zoothamnium sp.) 으로 나타났다. Diagnostic monitoring in fish farms with land-based tanks and netpen cases were conducted in eastern, western, southern and Jeju island of Korea during summer of 2007∼2011. In total, 2413-fish samples of 4 marine fish species were tested for the detection of bacteria and parasite. Fish species tested were olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), red sea bream (Pagrus major), pacific white shrimp (Litopenaeus vannamei). During the diagnostic monitoring from 2007 to 2011, the infection rates by single infection of bacterial or parasitic pathogens were relatively higher than the mixed infections. The main bacterial pathogens in olive flounder, black rockfish and pacific white shrimp were Vibrio spp. (V. harveyi, V. ichthyoenteri, Vibrio sp.). The main bacterial pathogens in red sea bream were also Vibrio sp. and Photobacterium damselae subsp. damselae. The main parasitic pathogens were both Miamiensis avidus and Trichodina sp. in olive flounder, Microcotyle sebastes in black rockfish, Microcotyle tai in red sea bream and Zoothamnium sp. in pacific white shrimp.

포르말린 약욕이 뱀장어 아가미흡충의 구제에 미치는 효과

정승희 ( Sung Hee Jung ),지보영 ( Bo Young Jee ),김진도 ( Jin Do Kim ),서정수 ( Jung Soo Seo ),김진우 ( Jin Woo Kim ) 한국어병학회 2010 한국어병학회지 Vol.23 No.3

Pseudodactylogyrus속 아가미흡충에 자연감염된 뱀장어(체중 89.8∼96g)를 대상으로 수온 28℃의 사육조건에서 포르말린의 구제효과를 알아보고자 실시하였다. 아가미흡충의 수적 변화에 의한 포르말린의 구제효과를 조사하기에 앞서, 포르말린의 농도별(0∼500 ppm) 및 시간별(30분∼24시간) 약욕에 따른 뱀장어의 헤마토크릿치(Ht) 변화를 분석하여 포르말린이 뱀장어의 혈액생리에 미치는 독성효과를 살펴보았다. 그리고 Ht 결과를 기준으로 하여 포르말린의 농도 및 처리시간 등을 약욕 처리법을 다양하게 설정한 다음 아가미흡충의 감염농도를 조사하였다. 포르말린에 24시간 동안 약욕한 뱀장어의 Ht는 대조구와 비교하여 100 및 200 ppm에서는 유의적인 차이가 없었다(P>0.05). 그러나 300∼500 ppm에서는 Ht가 유의적인 증가(P<0.05)를 나타내어 이 농도에서는 포르말린의 독성이 뱀장어의 생리기능에 영향을 초래하는 것으로 나타났다. 포르말린 100 및 200 ppm 농도에서 24시간 동안 약욕한 처리법에서 아가미흡충의 유의적인 감소(P<0.05)가 확인되었다. 한편, 연구자들은 앞선 연구에서 뱀장어에 대한 포르말린의 24시간 반수치사농도 (LC50)를 보고하였는데, 이 때 100 및 200 ppm에서 24시간 동안 약욕할 경우 200 ppm에서는 13.3%의 누적사망률을 확인하였으나, 100 ppm에서는 사망한 개체가 한 마리도 나타나지 않았다. 결론적으로 포르말린 100 ppm에서 24시간 동안 약욕하는 처리법이 뱀장어에 기생된 Pseudodactylogyrus속 아가미흡충을 효과적으로 구제할 수 있는 것으로 생각되었다. Effects of formalin on removal of Pseudodactylogyrus spp. were examined against naturally infected eel, Anguilla japonica (weight 89.9∼96g) at a water temperature of 28℃. Prior to experiments for removal of the parasite, the hematological toxicity of formalin bath at 0∼500 ppm for 30 min∼24 h was assessed by hematocrit values (Ht). Based on the results of Ht, appropriate methods of treatment, concentrations and durations, were examined in the main study. There was no significant (P>0.05) change of Ht in 100 to 200 ppm for 24 h. In contrast, Ht increased significantly (P<0.05) at above 300 ppm. This suggests that physiological damage was caused by formalin bath treating with 300 to 500 ppm. Formalin bath with 100 and 200 ppm for 24 h caused significant decreases (P<0.05) in the infection of the parasite. In conclusion, the 100 ppm formalin for 24 hour-bath was found most recommendable for the effective treatment of Pseudodactylogyrus spp. for the gills of the infected eel because of the median lethal concentration (LC50) of formalin to eel; cumulative mortalities were found to be 0 and 13.3%, respectively, following 24 h bathing.

Thiamphenicol의 단일 경구투여에 따른 넙치와 조피볼락의 혈액내 잔류 분석

정승희(Sung-Hee JUNG),서정수(Jung-Soo SEO) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.3

넙치(700±50 g, 23±1.5℃) 및 조피볼락(500±30g, 23±1.5℃)에게 Thiamphenicol(TP)을 1일 1회 경구(100 mg/kg BW) 투여한 다음, 경시적(1시간∼432시간)으로 혈청 내 TP의 잔류량을 HPLC로써 분석하였다. TP를 어류 혈청에 0.1, 1.0, 10 μg/mL으로 첨가한 각각의 농도에 대하여 넙치 및 조피볼락에서 TP의 평균 회수율은 77.05~97.23%와 89.96~97.11%로 나타났다. TP의 경구 투여에 따른 넙치와 조피볼락의 체내 약물 혈중농도는 two-compartment model로 조사되었다. TP를 투여 후 넙치 혈청에서 10시간째 10.08 μg/mL와 15시간째 10.06 μg/mL로 최대값을 보였고, 조피볼락 혈청에서는 15시간째 8.88 μg/mL로 최대값을 나타내었다. 넙치와 조피볼락의 혈청에서 TP는 투여 후 432시간째(18일째) 모든 시료에서 검출한계 이하로 검출되지 않았다. TP의 어류 체내 잔류 양상은 넙치와 조피볼락에서 매우 유사하였다 본 연구에서 . 얻어진 결과는 넙치와 조피볼락에 TP를 처방하여 치료 계획을 수립할 때 유용하게 활용될 것으로 여겨진다. This study was aimed at investigating the residual patterns of thiamphenicol (TP) in the cultured olive flounder (Paralichthys olivaceus) and black rockfish (Sebastes schlegeli) following oral administration. TP concentration were detected by high performance liquid chromatography with UV detector. The recovery rates of TP in serums ranged 77.05~97.23% (olive flounder) and 89.96~97.11% (black rockfish) for the concentration of 0.1, 1.0, 10 ㎍/mL, respectively. After single administration of TP (100 ㎎/㎏ body weight) by oral route in olive flounder (700±50 g, 23±1.5℃) and black rockfish (500±30 g, 23±1.5℃), the concentration in the serum was determined at 1, 5, 10, 15, 24, 30, 50, 168, 264 and 432 h post-dose. Two-compartment model was applied to analyze in the pharmacokinetics of TP administered to the fishes. In the serum of olive flounder, TP was detected on 10 and 15 hours after treatment were 10.08 ㎍/mL and 10.06 ㎍/mL as maximum level, respectively. In the serum of black rockfish, TP was detected on 15 hours after treatment were 8.88 ㎍/mL as maximum level. Concentrations of TP to the fishes were not measurable at 432 hours (18 days) after treatment in all samples. Similar residual patterns of TP were revealed between the fishes. These results are helpful for estimating withdrawal time of TP which has been already in use for farmed fish treatment.

조피볼락, Sebastes schlegeli에 경구투여된 nalidixic acid 및 piromidic acid의 약물동태에 미치는 수온의 영향

정승희 ( Sung Hee Jung ),서정수 ( Jung Soo Seo ),지보영 ( Bo Young Jee ),김진우 ( Jin Woo Kim ),박명애 ( Myoung Ae Park ) 한국어병학회 2011 한국어병학회지 Vol.24 No.1

Effects of temperature (13±1.5℃, 23±1.5℃) on the pharmacokinetic properties of nalidixic acid (NA) and piromidic acid (PA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of NA and PA were determined using HPLC-UV detector after a single dosage of 60 ㎎/㎏body weight. At 23±1.5℃, the peak serum concentrations of NA and PA, which attained at 24 h post-dose, were 5.87 and 0.43 ㎍/㎖, respectively. At 13±1.5℃, the peak serum concentrations of NA and PA, which attained at 10 h post-dose, were 6.22 and 1.57 ㎍/㎖, respectively. Better absorption of PA was noted at 13±1.5℃ compared to 23±1.5℃. However, absorption of NA was not affected significantly by temperature. The elimination of NA and PA from serum of black rockfish was considerably faster at 23±1.5℃ than at 13±1.5℃. The kinetic profile of absorption, distribution and elimination of NA and PA in serum were analyzed by fitting to a one compartment model, with WinNonlin program. The AUC, T1/2, Tmax and Cmax, respectively, were: 161.25 ㎍·h/㎖, 0.15 h, 12.29 h and 8.91 ㎍/㎖at 23±1.5℃, and 134.12 ㎍·h/㎖, 0.18 h, 8.79 h and 5.00 ㎍/㎖at 13±1.5℃ with NA; 41.57 ㎍·h/㎖, 0.58 h, 8.24 h and 0.21 ㎍/㎖at 23±1.5℃, and 40.36 ㎍·h/㎖, 0.59 h, 5.04 h and 1.20 ㎍/㎖at 13±1.5℃ with PA.

조피볼락, Sebastes schlegeli에 경구투여된 oxolinic acid의 약물동태에 미치는 수온의 영향

정승희 ( Sung Hee Jung ),김진우 ( Jin Woo Kim ),서정수 ( Jung Soo Seo ),지보영 ( Bo Young Jee ),박명애 ( Myoung Ae Park ) 한국어병학회 2010 한국어병학회지 Vol.23 No.2

Effects of temperature (13±1.5℃, 23±1.5℃) on the pharmacokinetic properties of oxolinic acid (OA) were studied after oral administration to cultured black rockfish, Sebastes schlegeli. Serum concentrations of OA were determined using HPLC-UV detector after a single dosage of 60 ㎎/㎏ body weight (average about 500 g). The peak serum concentrations of OA at 23±1.5℃ and 13±1.5℃ were 0.60 ㎍/㎖ at 30 h and 2.22 ㎍/㎖ at 10 h post-dose, respectively. Better absorption of OA was noted at 13±1.5℃ compared to 23±1.5℃. The elimination of OA from serum was considerably faster at 23±1.5℃ than at 13±1.5℃. Both absorption and elimination of OA were affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of OA in serum was analyzed by fitting to a two compartment model, with WinNonlin program. The AUC, Tmax and Cmax at 23±1.5℃ were 42.16 ㎍?h/㎖, 26.13 h and 0.43 ㎍/㎖, respectively. The AUC, Tmax and Cmax at 13±1.5℃ were 131.98 ㎍?h/㎖, 8.81 h and 2.04 ㎍/㎖, respectively.

총설 : 과망간산칼륨, 안정화이산화염소, 포르말린, 황산동이 넙치(Paralichthys olivaceus) 적혈구에 미치는 시험관내 용혈작용 및 메트헤모글로빈 생성 효과

정승희 ( Sung Hee Jung ),김진우 ( Jin Woo Kim ) 한국어병학회 2005 한국어병학회지 Vol.18 No.2

Oxolinic acid의 경구투여, 주사 및 약욕에 따른 넙치, Paralichthys olivaceus 체내 약물동태학적 특성

정승희 ( Sung Hee Jung ),최동림 ( Dong Lim Choi ),김진우 ( Jin Woo Kim ),조미라 ( Mi Ra Jo ),지보영 ( Bo Young Jee ),서정수 ( Jung Soo Seo ) 한국어병학회 2009 한국어병학회지 Vol.22 No.2