식물공장에서 재배한 브로콜리 새싹 용매 분획물의 항산화 활성

김은지,김미혜,Kim, Eun-Ji,Kim, Mi-Hye 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.1

This study was designed to determine the antioxidant activity of solvent fractions of broccoli sprouts grown by controlling the growing environment at the plant factory system. Fractionation was achieved with chloroform, n-hexane, ethyl acetate, butanol, water by 70% EtOH extract of the broccoli sprouts. Each solvent fraction was put through TLC and HPLC to separate active components. Higher antioxidant activities were observed for the butanol and ethyl acetate layers. Further evaluation of each of the 5 layers (LH1 to LH5) of the butanol fraction showed that the refined LH3 extract had a high antioxidant effect. Components with similar Rf values from TLC had the same retention times and peaks in the HPLC analysis. It was also determined that the sulforaphane content was high at the chloroform and butanol layers and the sulforaphane was responsible for, the high antioxidant activity. Thus, to use for functional materials, the butanol extract/layer of broccoli sprouts is recommended as the most effective.

유해인자별 노출실태 및 분석방법 26 - 실내공기 중 포름알데히드(HCHO) 분석의 정확도 및 재현성 평가

김은지,Kim, Eun-Ji 대한산업보건협회 2013 산업보건 Vol.306 No.-

개 정액의 냉동보존 시 κ-Carrageenan이 정자 성상에 미치는 영향

김은지,전유별,유일정,Kim, Eun-Ji,Talha, Nabeel A.H.,Jeon, Yu-Byeol,Yu, Il-Jeoung 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.1

This study was conducted to find out the effect that ${\kappa}-Carrageenan$ has on the properties of dog sperm when it was added to the cryoprotectant. Extender basically was contained 1.21 g Trizma base, 0.67 g citric acid, 0.4 g glucose, 0.03 g penicillin G, 0.05 g streptomycin sulfate. Extender1 was added with 0.1%, 0.2%, 0.3%, and 0.5% carrageenan, while extender2 was supplemented with glycerol. After freezing-thawing, the motility, viability, acrosome integrity, apoptosis, and ROS (reactive oxygen specifications) of sperm were measured to analyze the effects of the supplementation of carrageenan. Total Motile (TM), Rapid Progressive Motile (RPM), Medium Progressive Motile (MPM), and Immotile were measured through the CASA system after thawing in 37 degree water. Extender with 0.2% ${\kappa}-carrageenan$ ($64.26{\pm}0.49$) was significantly higher than control ($40.24{\pm}8.27$) (p < 0.05). RPMs of extender with 0.1%, 0.2% ${\kappa}-carrageenan$ ($57.64{\pm}6.34$, $56.47{\pm}1.35$) were significantly higher than the other groups (p < 0.05). Acrosome integrity was measured by dyeing to PSA-FITC with an epifluorescence microscope. Normal acrosome ratio of extender with 0.5% ${\kappa}-carrageenan$ ($61{\pm}8.03$) was higher than the other groups (p < 0.05). Apoptosis was measured with a FACSCalibur flow cytometer using FITC (FITC Annexin V Apoptosis Detection Kit). Treated groups of ${\kappa}-carrageenan$ of 0.1% ($0.81{\pm}0.05$), 0.2% ($0.85{\pm}0.05$) were significantly higer (p < 0.05) than control. Modified SYBR/PI staining was used for determination of viability and DCF staining was used for evaluation of ROS. Viability and ROS were not significantly different from other groups. In conclusion, adding a certain concentration of carrageenan to the extender of cryopreservation, carrageenan contributes to the improvement of the sperm motility, acrosome integrity and prevention of apoptosis.

조등산(Diaoteng-san, Choto-san, 釣藤散)과 GB34(陽陵泉) 전침치료를 활용한 약인성파킨슨증후군 환자의 증례 1례

김은지,송호준,김현호,한양희,임정태,Kim, Eun-ji,Song, Ho-joon,Kim, Hyun-ho,Han, Yang-hee,Leem, Jung-tae 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.5

Drug-induced Parkinsonism is the most frequently observed type among the cases of secondary Parkinsonism. Besides typical parkinsonian symptoms, such as tremor, rigidity and bradykinesia, drug-induced Parkinsonism manifests with additional simultaneous symptoms like orobuccolingual dyskinesia, mixed type of tremor (resting, action), and symmetry of expressions. We present a case of drug-induced Parkinsonism, affected by taking the antiulcer drug cimetidine. Jodeung-san extract (Choto-san, Tsumura Co. 10) was administered for 7 days and acupuncture (electronic, auricular, pharmacopuncture) was conducted 3 times. The clinical outcomes were then evaluated through the patient's global impression of change, visual analogue scale, and Hoehn and Yahr stage. After the treatment, the clinical features, such as tremor and orobuccolingual dyskinesia, disappeared. The combination of Jodeung-san and electro-acupuncture at GB34 could therefore be a remedy for the patients with drug-induced Parkinsonism.

경력개발과 번영감, 그리고 잡크래프팅 간의 관계

김은지 ( Eunji¸ Kim ),문재승 ( Jaeseung¸ Moon ) KNU기업경영연구소 2021 기업경영리뷰 Vol.12 No.3

최근 국내에서도 조직행동분야에서 번영감에 대한 논의가 점차 관심을 받고 있다. 번영감이란 조직 구성원이 업무수행과정에서 지각하는 활력감, 학습감이 결합된 개념을 말한다. 번영감의 긍정적인 영향력 때문에 연구자와 실무에서 번영감에 관한 관심이 높고, 이에 따라 관련 연구가 지속되고 있으나, 여전히 선행요인과 결과요인, 그리고 메커니즘을 탐색하기 위한 연구가 요구되고 있다. 이에 따라 본 연구는 선행연구에서 다루지 않았던 번영감의 선행 요인과 결과영향을 탐색하고자 한다. 본 연구는 경력관리행동과 조직경력개발지원을 번영감의 선행요인으로 잡크 래프팅을 번영감의 결과요인으로 연구모형을 설계하고 실증하였다. 실증분석을 위해 국내 기업에서 근무하는 477 명의 종업원을 대상으로 설문조사를 실시하였다. 실증분석결과는 다음과 같다. 첫째, 경력관리행동은 번영감에 정 (+)의 영향을 미치는 것으로 나타났다. 둘째, 조직경력개발지원은 번영감에 정(+) 영향을 주는 것으로 확인되었다. 셋째, 번영감이 잡크래프팅에 정(+)의 영향을 미쳤다. 넷째, 번영감은 경력관리행동과 잡크래프팅 간의 관계와 조직경력개발지원과 잡크래프팅 간의 관계를 매개하는 것으로 나타났다. 본 연구의 실증 결과를 바탕으로 연구의 시사점과 한계 및 향후 연구방향을 논의하였다 Recently, ‘thriving at work’ has been receiving attention in the field of organizational behavior. Thriving at work refers to a concept that combines the sense of vitality and learning that employees perceive in the process of performing tasks. Researchers and practitioners are interested in ‘thriving at work’ and related research is con-tinuing because of the positive impact of it. However, there is still a need for research to explore the anteced-ents, consequences, and mechanisms. Thus, this study intends to explore the antecedents and consequences of ‘thriving at work’ that were not addressed in previous studies. Specifically, this study designed and demonstrated a research model in which career management behavior and organizational career development support were antecedents for thriving at work and job crafting was a consequence of it. For empirical analysis, a survey was conducted with 477 employees working in domestic companies. The empirical analysis results are as follows. First, career management behavior was found to have a positive effect on the thriving at work. Second, it was confirmed that organizational career development support had a positive effect on the thriving at work. Third, thriving at work had a positive effect on job crafting. Fourth, thriving at work was found to mediate the rela-tionship between career management behavior and job crafting, and between support for organizational career development and job crafting. Based on the empirical results of the study, the implications and limitations of the study and future research directions were discussed.

보라우무 메탄올추출물의 HT-29 대장암세포 증식 억제 효과

김은지,박소영,홍지은,신민정,임순성,신현경,윤정한,Kim, Eun-Ji,Park, So-Young,Hong, Ji-Eun,Shin, Min-Jeong,Lim, Soon-Sung,Shin, Hyun-Kyung,YoonPark, Jung-Han 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.4

해조류는 다양한 생리 활성을 나타내는 성분들을 함유하고 있어 항산화효과 등 다양한 생리활성 효과를 나타내는 것으로 보고되고 있다. 본 연구에서는 식용 가능한 28종의 해조류 메탄올추출물 중 보라우무 메탄올추출물이 인간의 대장에서 유래한 암세포인 HT-29세포의 세포증식을 억제함을 밝혔고, 가장 강한 HT-29 세포 증식 억제 효과를 나타낸 보라우무 메탄올추출물의 대장암세포 증식억제 기전을 밝히고자 하였다. 보라우무 메탄올추출물을 HT-29 세포 배양액에 여러 농도$(0{\sim}20{\mu}g/mL)$로 첨가하여 세포를 배양한 경우 보라우무 메탄올추출물 처리 농도가 증가할수록 세포증식이 현저히 감소하였고, apoptotic cell수는 현저히 증가하였다. Apoptosis의 주요한 조절인자인 Bcl-2 family 단백질 중 Bcl-2는 보라우무 메탄올추출물에 의해 변화가 없었고 Bax는 고농도에서만 소폭 감소하였다. 반면, t-Bid 단백질 수준은 보라우무 메탄올추출물의 처리에 의해 현저하게 증가하였다 Bcl-2 family Protein과 더불어 apoptosis의 조절에 주요한 역할을 하는 caspase 단백질의 경우 보라우무메탄올추출물 처리에 의해 cleaved caspase-8, -9, -7, -3 단백질 수준이 현저히 증가하였고, cleaved PARP 단백질수준도 현저히 증가하였다. 또한, caspase-8의 활성화를 유도하는 Fas 단백질 수준도 보라우무 메탄올추출물에 의해 현저하게 증가하였다. 이 결과로부터 보라우무 메탄올추출물은 caspase-8의 활성 증가에 의한 미토콘드리아 막 투과성 변화와 caspase-7/-3 활성의 증가를 통해 apoptosis를 유도함으로써 암세포의 증식을 억제한다는 결론을 내릴 수 있다. 본 연구는 보라우무를 항암효과를 지니는 기능성식품이나 항암제로 개발할 수 있는 가능성을 제시하고 있으며 향후 보라우무를 이용한 제품의 개발을 위해서는 유효성분의 동정 및 그 성분의 작용 기전에 대한 면밀한 추가 연구가 필요할 것으로 보인다. In the present study, twenty eight marine algae species were evaluated for their antiproliferative effect on HT-29 human colon cancer cells. Among these, the methanolic extract of Symphyocladia latiuscula (SL Ex) showed the highest inhibitory activity on HT-29 cell growth. In this study, we examined the mechanism by which SL Ex inhibited the HT-29 cell growth. Cells were cultured with various concentrations of $(0{\sim}20{\mu}g/mL)$ SL Ex. The SL Ex substantially decreased the viable cell numbers and induced apoptosis of HT-29 cells in a dose-dependent manner Western blot analyses of total cell lysates revealed that SL Ex increased the levels of cleaved caspase-8, -9, -7, and -3, and poly (ADP-ribose) polymerase in HT-29 cells. In addition, SL Ex increased truncated Bid levels but moderately decreased Bax levels at only $20{\mu}g/mL$. Furthermore, SL Ex did not affect Bcl-2 protein levels but increased the levels of Fas in HT-29 cells. The present results indicate that SL Ex inhibits cell growth via inducing apoptosis in human colon cancer cells. The mechanism of apoptosis induction by SL Ex involves caspase-8 activation leading to changes in mitochondrial events and subsequent activation of the caspase-7/caspase-3 cascade. Our finding may lead to the development of new therapeutic strategies for the treatment of colon cancer.

간다거리의 스포츠 전문서점 '쇼센 북마트'

김은지,Kim, Eun-Ji 대한출판문화협회 1998 출판저널 Vol.238 No.-

올해로 개점 31년째인 '쇼센북마트'는 지하 1층 지상 4층에 스포츠 관련서와 비디오테이프.CD를 갖추고 있다. 축구.야구 등 인기종목뿐만 아니라 합기도.유도.태극권 등 격투기 같은 희귀종목 관련서는 타의 추종을 불허한다. 또 모든 스포츠 잡지의 과월호를 모두 갖추고 있어 스포츠광들의 발길이 끊이지 않는다.

합성피혁 스킨용 폴리우레탄 수분산체의 제조 및 primerless 접착 특성 연구

김은지,백인규,박재형,Kim, Eun Ji,Paik, In Kyu,Park, Jae Hyung 한국섬유공학회 2017 한국섬유공학회지 Vol.54 No.1

Shoes, made up of an upper shoe, insole, and outsole, are manufactured by adhering each of the parts together. It has been proven that pre-treating each of these parts greatly improves the adhesive strength when adhering them together. However, harmful materials such as volatile organic compounds (VOCs) are often emitted from organic solvents during pre-treatment, causing environmental concerns such as air pollution in the workplace. Therefore, this study was conducted to develop a water-based polyurethane skin resin for fabricating a synthetic leather and textile adhesive that has greater adhesive strength and does not require pre-treatment. To achieve this, two waterborne polyurethane solutions, one with excellent thermal resistance and light fastness (waterborne polyurethane A) and the other with high adhesive strength (waterborne polyurethane B), were mixed to impart the superior characteristics of each to a third waterborne polyurethane solution. Through this approach, we were able to successfully fabricate an excellent waterborne leather polyurethane resin with ethylene vinyl acetate (EVA) for midsoles (peel strength: 2.9 kgf/cm) and for rubber out-soles (peel strength: 2.4 kgf/cm) without using pre-treatment.

해외 서점 탐방-일반 유통을 거치지 않은 모든 책이 '모사쿠샤' 서점

김은지,Kim, Eun-Ji 대한출판문화협회 1998 출판저널 Vol.236 No.-

유통기구를 통하지 않는 모든 책들을 갖춘 '모사쿠샤' 서점은 출판사와 단체, 독자 모두에게 환영받고 있다. 10평 남짓한 공간에 반핵.노동.장애인, 한국.북한관련서, 대학신문과 팜플렛 등이 주제별로 빼곡하게 들어차 있다.

항암 치료 후 발생한 수족증후군에 대한 한의학적 치료의 효과에 대한 고찰

김은지,박찬란,손창규,조정효,이남헌,Kim, Eun-ji,Park, Chan-ran,Son, Chang-gue,Cho, Jung-hyo,Lee, Nam-hun 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.3

Objectives: Hand-foot syndrome (HFS) is a common adverse effect of chemotherapy in cancer patients. This review synthesizes research results to assess the effect of traditional Korean medicine (TKM) on HFS in cancer patients. Methods: Four databases (PubMed, The Cochrane Central Register of Controlled Trials, Research Information Sharing Service, China National Knowledge Infrastructure) were searched for randomized controlled trials (RCTs) to assess the effects of TKM on HFS. The effects and quality of RCTs were assessed with the Cochrane risk of bias (ROB) tool. Results: Six RCTs met our study criteria. In all six, TKM showed improvement in HFS symptoms as well as quality of life scores when compared to the control group. However, the methodological quality of RCTs was relatively low due to the unclear or high ROB. Conclusions: TKM would be helpful to patients with HFS after chemotherapy. To clarify the clinical efficacy of TKM, well-designed studies are required in the future.