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김원극,김자영,백태경,백두진,정호삼,이규식,Kim, Won-Kyu,Kim, Ja-Young,Baik, Tai-Kyeoung,Baik, Doo-Jin,Chung, Ho-Sam,Lee, Kyu-Sik 한국현미경학회 1993 Applied microscopy Vol.23 No.1
To investigate the histogenesis of tracheal epithelium in Sprague-Dawley strain rat, the author has used the fetal rats at the 16th, 18th, 20th and 22nd prenatal day and neonatal rats at the 1st and 7th day as well as rats at age of 5, 10 and 15 weeks after birth as experimental animals. Specimens were double stained with uranyl acetate and lead citrate for electron microscopic study. The results obtained were as follows; 1. At the 16th day of gestational age, ciliated cells were found in tracheal epithelium and light and dark ciliated cells possessing numerous mitochondria and smooth endoplasmic reticulum in the cytoplasm at the 22nd day of gestational age of the rat are observed. 2. At the 16th day of gestataional age, basal cells lying upon the basement membrane and having large numbers of glycogen particles in the cytoplasm, were found and at the 22nd day of gestational age, basal cells possessing numerous polysomes in the cytoplasm were observed. 3. At the 20th gestational age of the rat, microvillous cells possessing many rough endoplasmic reticulum and mitochondria as well as microvilli protruding into the lumen were found in tracheal epithelium. 4. At the 5th week after birth brush cell having profound filamentous strands and many pinocytic vesicles in the cytoplasm, was visible in the tracheal epithelium. 5. At the 15th week after birth large proportions of tracheal epithelium were lined with ciliated cells. Cosequently it is suggested that pseudostratified ciliated columnar epithelium was differentiated at the 16th day of gestational age, in addition cytoplasmic organelles of the microvillous and basal cells were matured at the 20th and 22nd gestational age, respectively and most of the part of the tracheal epithelium was lined with ciliated cells at the 15th week after birth.
Cantinuous Process for Sorbitol Production with Zymomonas mobilis
Kim, Won-Keuk,Kim, In-Chul,Chun, Uck-Han 慶熙大學校 1990 論文集 Vol.19 No.-
Ethnol을 주로 생산하는 균주인 Z. mobilis의 cell wall 투과성을 높인 후 고정화 하여 sorbitol 생산에 이용 하였다. Glucose-fractose oxidoreductase의 specificity를 위한 투과성을 높이는데는 toluene이 가장 효과적이었으나, 3회 연속으로 Batch process를 행하였을 때 oxidoreductase의 유출, 소실로 인하여 sorbitol conversion efficiency가 급격히 저하되었다. 투과성을 높인 cell을 alginate에 고정화 하여 continuous process를 행하였으나, 역시 효소활성도는 장시간 지속되지 못하였다. 따라서, cell을 0.25% glutaraldehyde로 처리한 후 alginate에 고정화 하여 희석비율 0.18h^-1에서 continuous process를 한 결과 210시간동안 82%의 효소활성도가 유지되었다. 또한, glutaraldehyde와 chitin을 동시에 고정화 supporter로 사용하였을때 거의 90%의 효소활성도가 210시간동안 유지되었으며, 특히 alginate만을 사용했을때 보다 더 높은 bead안정성을 나타내었다.
Zymomonas mobilis와 Gluconobacter suboxydans를 이용한 돼지감자로부터 D-sorbitol 및 L-sorbose 생성에 관한 연구
전억한,김원극 한국생물공학회 1993 KSBB Journal Vol.25 No.1
The use of Jerusalem artichoke containing $\beta$-1, 2-fructose oligomer for the production of D-sorbitol and L-sorbose has been studied. The employment of inulinase(0.398%, v/v) for the hydrolysis of 40% (v/w) Jerusalem artichoke juice resulted in 36.7g/1 of glucose and 85.3g/1 of fructose at $50^{\circ}C$. These sugars were utilized as substrates for D-sorbitol and L-sorbose production. Coimmobilization of inulinase and permeabilized cells of Zymomonas mobilis in the mixture of chitin (5%, w/e) and x-carrageenan(4%, w/v) resulted in the production of 30.2g/1 of D-sorbitol by using inulin as a substrate. The process of L-sorbose production from D-sorbitol by Gluconobacter suboxydans was optimized with respect to the substrate concentration, level of dissolved oxygen and glucosic and concentration. Gluconlc acid produced by Zymomonas mobilis from glucose was found to inhibit Gluconobacter suboxtans in conversion of D-sorbitol to L-sorbose. In view of removing such inhibitory effect by gluconic acid, mutants were selected by the NTG (N-methyl-N'-N'-nitro-N-nitrosoguanidlne) treated method. Mutants selected by NTG mutagenesis showed no inhibitory effects of gluconic acrid against L-sorbone production when its concentration increased up to 100g/1. A mutant produced 40.1g/l of L-sorbose in the medium containing 100g/l D-sorbitol and 100g/l-gluconic acid. This result is consider able when compared with L-sorbose concentration (21.7g/1) obtained from the fermentation with wild type strain of Gluconobacter suboxnians.
Choi, Do-Jin,Kim, Won-Keuk,Chun, Uck-Han 경희대학교 유전공학연구소 1990 遺傳工學論文集 Vol.2 No.-
Bthanol을 주로 생산하는 균주인 .Z mobths의 cell wall 투과성을 높인 후 고정하여 sorbitol 생산에 이용하였다. 그러나, toluene으로 투과성을 높인 cell은 oxido-teductase의 유출 손실로 인하여 sorbitol conversion efficiencv가 급격히 저하되었다. 따라서, 이와같은 enzveme의 유출을 방지 하기 위하여, 투과성을 향상시킨 cell을 0.25% glutaraldehvde로 처리한 후 alginate와 chitm에 고정화하여 희석율 0.2h^(-1)에서 연속배양을 한 결과 210시간동안 효소활성도의 저하는 거의 일어나지 않았다. 이와같은 연속배양에서 얻어진 sorbitol productivitv는 3.5g/lh로 측정되었다. This study describes the sorbitol production with permeabilized cells of Zymomonas mobilis immobilized in Ca-alginate. Toluene treated cells lose glucose-fructose oxidoreductase activity due to leaking of enzyme from the cells. In order to prevent this leakage, the permeabilized cells were immobilized in alginate and chitin. No significant loss of enzyme activity was apparent during 210h operation in a continuous process. The productivity of the continuous process was estimated to be about 3.5g/l-h for sorbitol at dilution rate 0.2h^(-1).
김용택,김원극,오훈일 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.1
우리나라의 전통식품인 청국장과 일본의 전통 발효발효식품인 natto로부터 혈전용해 균주를 분리하여 혈전용해능을 조사한 결과 청국장에서 분리된 균주중에는 혈전용해능을 나타내지 않는 균주가 다수 포함되어 있으나, natto로부터 분리한 균주들은 대부분 혈전용해능을 나타내었다. 그러나 가장 우수한 혈전용해능을 나타내는 균주는 청국장에서 발견되었으며, 그 활성은 1.84 plasmin unit였다. 이 균주의 동정을 위하여 형태학적, 생화학적 특성을 조사한 결과 분리균주는 간균으로 운동성이 있고, 호기성(catalase 함유)으로 그람양성의 세균이었으며 포자를 형성하는 것으로 관찰되어 Bacillus 속에 속하는 미생물로 동정되었으므로 Bacillus sp. CK 11-4로 명명하였다. Bacterial strains showing the fibrinolytic activity were screened from Chungkook-jang and Natto (Japanese traditional soy food). The strains isolated from Natto revealed a high level of fibrinolytic activity, wherease nearly half of the isolates from Chungkook-jang did not show the relevant activity. However, one strain isolated from Chungkook-jang showed the highest fibrinolytic activity (1.84 plasmin unit), and subsequently identified as Bacillus species. The fiorinolytic strain was designated as Bacillus sp. CK 11-4.