혈구탐식성 림프조직구증의 임상적 특징과 생존에 미치는 예후인자

김기주,김기환,이택진,전진경,유철주,김동수,Kim, Khi Joo,Kim, Ki Hwan,Lee, Taek Jin,Chun, Jin-Kyong,Lyu, Chuhl Joo,Kim, Dong Soo 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.3

Purpose : Hemophagocytic lymphohistiocytosis (HLH) is a rare but fatal disorder characterized by fever, splenomegaly, pancytopenia, and hemophagocytosis in the bone marrow and other tissues. In this study, we investigated the clinical manifestations and prognostic factors in patients with HLH. Methods : We retrospectively analyzed the data from 29 patients who were diagnosed whit HLH in the Severance Children's Hospital from Jan. 1996 to Feb. 2007. Results : The median age at diagnosis was 3.8 years (range 0.1-12.2). The ratio of male to female patients was 1.1:1. The 5-year overall survival rate was 55.2% with a median follow-up duration of 32 months. In a multivariate analysis, the duration of fever before admission (survival vs. non-survival, 6.5 days vs. 14 days, P=0.010), the interval from the day of fever onset to the day of initiation of etoposide (survival vs. non-survival, 10 days vs. 35 days, P=0.002) and the presence of neurologic symptoms (survival vs. non-survival, 1 case vs. 7 cases, P=0.010) were independent, poor prognostic factors of HLH. EBV infection, gender, and the level of serum ferritin had no relations to the poor prognosis of the disease. Conclusion : This study showed that the presence of neurologic symptoms and a longer duration of fever were related to a poor prognosis. Therefore, if a patient develops neurologic symptoms and the duration of fever is prolonged, a prompt diagnostic approach and aggressive treatment for HLH are necessary. 목 적 : HLH은 발열, 범혈구감소증, 비종대, 골수에서의 혈구탐식을 특징으로 하는 질환으로 생존율이 낮고 급격한 진행양상을 보이는 비교적 드문 질환으로 생존율에 영향을 미치는 인자들을 알아보고자 본 연구를 시행하였다. 방 법 : 1996년 1월부터 2007년 2월까지 세브란스 어린이 병원에 내원하여 HLH로 진단된 환아들을 대상으로 진료기록을 토대로 후향적으로 연구를 진행하였다. 결 과 : HLH로 진단된 환아는 총 29명이었고(남아 15명, 여아 14명) 연령의 중앙값은 3.8(0.1-12.2)세였다. 가족력이 있었던 환아는 2례였다. 29명 환아의 5년 생존율은 55.2%였다. 생존군과 사망군의 통계학적으로 유의한 차이가 있었던 소견으로는 입원전 발열기간, 처음 발열일부터 etoposide를 투여시작까지의 기간 및 신경학적 이상 유무가 있었다. 입원전 발열기간은 생존환아에서는 6.5(2-21)일이었고 사망환아에서는 14(5-60)일이었으며(P=0.010) 처음 발열일부터 etoposide를 사용하기까지의 기간은 생존환아에서는 10(8-23)일, 사망환아에서는 35(15-84)일이었다(P=0.002). 또한 신경학적 이상은 생존환아에서는 1례 있었고 사망환아에서는 7례가 있었다(P=0.010). 결 론 : HLH은 발열을 주증상으로 간비비대나 범혈구 감소증, 경련이나 의식의 장애와 같은 신경학적 증상을 동반할 수 있는 질환으로 환아가 발열기간이 길거나 신경학적 증상이 발병할 경우에는 HLH의 예후가 나쁘므로 HLH 의심 시 신속한 진단적 접근과 함께 조기의 적극적인 치료가 필요할 것으로 생각된다.

Development of automotive engine cradle by hydroforming process

김기주,J. S. Kim,B. I. Choi,K. H. Kim,H. H. Choi,C. W. Kim,K. W. Kang,J. H. Song,C. W. Sung 대한기계학회 2007 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.21 No.10

The hydroforming technology may bring many advantages to automotive applications in terms of better structural integrity of the parts, lower cost from fewer part count, material saving, weight reduction, lower springback, improved strength and durability and design flexibility. In this study, the whole process of front sub-frame parts development by tube hydroforming using steel material having tensile strength of 440 MPa grade is presented. At the part design stage, it requires feasibility study and process design aided by CAE (Computer Aided Design) to confirm hydroformability in details. Effects of parameters such as internal pressure, axial feeding and geometry shape in automotive engine cradle by the hydroforming process were carefully investigated. Overall possibility of hydroformable engine cradle parts could be examined by cross sectional analyses. Moreover, it is essential to ensure the formability of tube material on every forming step such as pre-bending, preforming and hydroforming. At the die design stage, all the components of prototyping tool are designed and interference with the press is examined from the point of deformed geometry and local thinning.

자동차용 알루미늄 5185-폴리프로필렌 샌드위치 판재의 성형성

김기주,정효태,손일선,김철웅,김중배,Kim, Kee-Joo,Jeong, Hyo-Tae,Sohn, Il-Seon,Kim, Cheol-Woong,Kim, Joong-Bae 한국자동차공학회 2007 한국 자동차공학회논문집 Vol.15 No.2

The objective of this study was to develop formability evaluation techniques in order to apply aluminum sandwich panel for automotive body parts. For this purpose, newly adopting formability evaluation (using limit dome height and plane strain test) was carried out in order to secure the fundamental data for the measurement of sheet metal forming and the establishment of optimum forming conditions of the aluminum sandwich panel. The results showed that there were good agreements between the old formability evaluation method and the new method which was more simplified than that of old one. From the results of these formability evaluation, the formability of sandwich panel was higher than that of aluminum alloy sheet alone which was the skin component for the sandwich panel. Also, it was found that sandwich panel could reduce the weight and could have the same flexural rigidity simultaneously when it was compared to the automotive steel sheet.

Salmonella enterica serovars Enteritidis의 온도감수성 변이주 및 폴리인산키나아제 변이주의 제작과 방어효과

김기주,박소연,조영재,곽정연,강정무,김은희,최환원,원호근,노윤희,한태욱,Kim, Kiju,Park, Soyeon,Cho, Youngjae,Kwak, Jeong-Yeon,Kang, Zheng-Wu,Kim, Eun-Hee,Choi, Hwan-Won,Won, Ho-Keun,Noh, Yun-Hee,Hahn, Tae-Wook 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.4

This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.

정상 한우 및 번식장애 한우에 대한 자궁 내 세균 분석

김기주,박소연,조영재,정배동,박정준,한태욱,Kim, Kiju,Park, Soyeon,Cho, Youngjae,Jung, Bae-Dong,Park, Joung-Jun,Hahn, Tae-Wook 대한수의학회 2014 大韓獸醫學會誌 Vol.54 No.4

Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.

최근 국내에서 유행하는 돼지 유행성 설사 바이러스 nucleocapsid 유전자의 다양성 및 계통 분석

김기주,박유경,박보경,람쯩꽝,박소연,김재훈,한태욱,Kim, Kiju,Park, Yookyung,Park, Bokyung,Truong, Quang Lam,Park, Soyeon,Kim, Jaehun,Hahn, Tae-Wook 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.1

Porcine epidemic diarrhea virus (PEDV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea in piglets, resulting in large economic losses because of high mortality. In November 2013, PEDV reemerged in Korea, and these outbreaks have since continuously occurred. In the present study, we determined the full-length nucleocapsid (N) gene sequences of three Korean PEDV field isolates collected in 2014-2015. Sequence and phylogenetic analysis of N genes revealed that recent prevalent Korean PEDV isolates were very closely related to the US PEDV isolates in 2013. Interestingly, the phylogenetic tree based on the nucleotide sequencing of the PEDV N gene was similar to the tree topology of the PEDV complete genomes. Therefore, our data provide a better understanding of the genetic diversity and contribute to the accurate diagnosis and development of vaccines against PEDV.

비스페놀 A 형 에폭시수지의 Corrosion 및 Erosion 에 관한 연구

김기주,김동섭 ( K . J . K im,D . S . Kim ) 한국부식방식학회 1992 Corrosion Science and Technology Vol.21 No.1

Salmonella enterica serovars Enteritidis, Gallinarum 및 Typhimurium의 마우스 면역반응의 비교평가

김기주,김두리,선지선,박소연,조영재,고현정,주홍구,한태욱,Kim, Kiju,Kim, Dooree,Sun, Jisun,Park, Soyeon,Cho, Youngjae,Ko, Hyun-Jeong,Joo, Hong-Gu,Hahn, Tae-Wook 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.2

The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.

돼지 파보바이러스, 단독 및 렙토스피라 8가 불활화 백신의 안전성 및 면역원성 평가

김기주,최종영,박수진,한태욱,Kim, Kiju,Choi, Jong-Young,Park, Su-Jin,Hahn, Tae-Wook 대한수의학회 2017 大韓獸醫學會誌 Vol.57 No.3

Porcine parvovirus, Erysipelothrix (E.) rhusiopathiae, and Leptospira (L.) interrogans are considered major etiologic agents of reproductive failure in pigs, causing economic loss in the swine industry. In this study, the safety and immunogenicity of a new octavalent inactivated vaccine were evaluated. The vaccine contained inactivated porcine parvovirus, E. rhusiopathiae, and six L. interrogans serovars (Bratislava, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae, and Pomona). Safety test results showed no notable side effects or clinical signs after vaccination in mice, guinea pigs, and sows. In addition, we assessed immunogenicity of the vaccine in 25 sows under field conditions. The vaccinated group (n = 20) had a significantly higher antibody level than the non-vaccinated group (n = 5). Moreover, the stillbirth rate decreased in piglets born from vaccinated sows, resulting in an increased fertility rate. The results of this study demonstrate that the new octavalent inactivated vaccine can be applied safely and effectively to improve reproductive performance in sows.

꽁치의 어업생물학적 연구 3. 수량변동

김기주,KIM Ki-Joo 한국수산과학회 1973 한국수산과학회지 Vol.6 No.1,2

우리나라 동안의 꽁치에 대하여 $1964\~1973$년의 어획통계 및 $1968\~1972$년의 체장조성자료를 이용하여 수량변동을 검토한 결과는 다음과 같다. 1. $1964\~1972$년에 있어서 어느 해의 가을(9월$\~$익년 2월)의 자원량과 다음 해의 봄(3$\~$8월)의 자원량과의 사이에는 순상관이 보이며, 봄 및 가을의 자원량은 각각 3년 주기로 변화되며, 가을의 자원량의 변동주기는 봄의 자원량의 변동주기 보다 1년 빠르게 Mode가 나타나고 있다. 2. 봄에 있어서 1967년에는 노력량이 과다하며, 1969년에는 노력량이 과소하고, 1972년에는 어장의 이용도가 낮은 것 같으며, 가을에서는 1967년에 노력량이 과다하고, 1969년에는 어장의 이용도가 낮은 것 같다. 그리고 가을의 노력량은 더욱 증가시킬 수 있을 것이라 생각된다. 3. 봄에 있어서 대형군의 비율이 높은 해에는 자원량이 많으며, 가을에서는 중형량이 대형량보다 많은 해에는 자원량이 많은 경향이 있는 것 같다 4. 봄의 북상기에 있어서 각 월의 어군분포대심이 외해에 있고, 북방향으로 이동하는 해에는 어획량이 많으며 어군분포중심이 연안으로 기울어지고, 서 또는 북서방향으로 이동하는 해에는 어획량이 적은 경향이 있는 것이라 추정된다. Changes in stock size of the Pacific saury, Cololabis saira, were studied on the basis of fishery statistics compiled from 1964 to 1972 and body size composition of the fish collected from 1968 to 1972. During the period from 1964 to 1972 there was a direct correlation between the stocks of fall (september-february of the following year) and spring (March-August) season. The sizes of stocks in both seasons showed a three-year cyclic change, and the mode of stock in the fall always proceeded one year that in the spring. Exceedingly high fishing effort was observed in the spring as well as the fall of 1967. But very low fishing effort was noticed in the spring of 1969. In spring a large stock size has a high proportion of large sized group. On the other hand, in fall a large stock size tends to have a high proportion of medium sized group instead. When the medium sized group outnumbers the large sized group, stock size becomes larger. In contrast, it tends to be smaller when the large sized group exceeds the medium sized group. The patterns of distribution centroid seems to be related to the amount of fish landings. Northward moving trend of the centroid was accompanied by a large amount of landing, while westward (or north-westward) moving trend was followed by a poor landing of the fish.