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최근 국내에서 유행하는 돼지 유행성 설사 바이러스 nucleocapsid 유전자의 다양성 및 계통 분석
김기주,박유경,박보경,람쯩꽝,박소연,김재훈,한태욱,Kim, Kiju,Park, Yookyung,Park, Bokyung,Truong, Quang Lam,Park, Soyeon,Kim, Jaehun,Hahn, Tae-Wook 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.1
Porcine epidemic diarrhea virus (PEDV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea in piglets, resulting in large economic losses because of high mortality. In November 2013, PEDV reemerged in Korea, and these outbreaks have since continuously occurred. In the present study, we determined the full-length nucleocapsid (N) gene sequences of three Korean PEDV field isolates collected in 2014-2015. Sequence and phylogenetic analysis of N genes revealed that recent prevalent Korean PEDV isolates were very closely related to the US PEDV isolates in 2013. Interestingly, the phylogenetic tree based on the nucleotide sequencing of the PEDV N gene was similar to the tree topology of the PEDV complete genomes. Therefore, our data provide a better understanding of the genetic diversity and contribute to the accurate diagnosis and development of vaccines against PEDV.
개 혈액 재료에서의 Brucella 검출을 위한 진단방법의 비교
권순오 ( Soon Oh Kwon ),람쯩꽝 ( Truong Quang Lam ),허문 ( Moon Her ),안동춘 ( Dong Chun Ahn ),박상희 ( Sang Hee Park ),박미연 ( Mi Yeoun Park ),이영주 ( Young Ju Lee ),한태욱 ( Tae Wook Hahn ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4
Canine brucellosis produce abortions and infertility in dogs and currently is diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.
도축돈에서 분리된 Bordetella bronchiseptica의 항생제 감수성 조사
신은경 ( Eun Kyung Shin ),아비히지트바레이트 ( Abihijit K. Barate ),람쯩꽝 ( Truong Quang Lam ),홍종해 ( Chong Hae Hong ),한태욱 ( Tae Wook Hahn ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.3
The antimicrobial susceptibility of the 41 Bordetella bronchiseptica isolates was tested by using the Kirby-Bauer agar disk diffusion method. The B. bronchiseptica isolates were found to be sensitive to amoxicillin/clavulanic acid (100%), gentamicin (100%), neomycin (100%) and amikacin (97.6%), whereas they were resistant to streptomycin (100%), trimethoprim/sulfamethoxazole (100%), penicillin (100%) and ampicillin (97.6%). All the B. bronchiseptica isolates resisted to at least 4 antimicrobial agents and totally 8 different combinations of multiple antibiotic resistance patterns were noted. All of the B. bronchiseptica isolates, except one, were simultaneously resistant to streptomycin, trimethoprim/sulfamethoxazole, penicillin and ampicillin. The observed antibiotic resistance is not plasmid mediated as plasmids were absent from all the B. bronchiseptica isolates.