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Expression of Anion Exchanger and CFTR in the Hepatocyte and Cholangiocytes in Bile Duct-Ligated Rat
Jae Dong Lee(이재동),Joon Ho Wang(왕준호),Seung Seog Ki(기승석),Won Hyeok Choe(최원혁),Jae Seung Park(박재승),Won Kyoo Cho(조원규),Jung-Jun Park(박정준),Hong Su Kim(김홍수) 한국생명과학회 2011 생명과학회지 Vol.21 No.12
본 연구는 흰쥐의 담관 세포와 간세포에서 CFTR과 AE1 AE2 AE3 유전자들의 발현 유무를 조사하고 흰쥐에서 담관 결찰 후 AE2 유전자의 발현의 변화를 관찰하고자 하였다. 200-250 g의 Sprague Dawley 계 흰쥐 24마리의 총담관을 결찰한 후 4 주 동안 1 주일에 6마리씩 희생하여 간세포와 담관 세포를 분리하였다. 6마리는 대조군으로 사용하여 간세포와 담관 세포에서 CFTR 유전자와 AE1 AE2 와 AE3 유전자 발현을 조사하고 담관 결찰 후 1 2 3 4주 간격으로 AE2 유전자 발현을 조사하였다. AE1 AE2 와 AE3는 간세포와 담관 세포에서 발현되었고 CFTR은 담관 세포에서만 발현되었다. 담관 결찰 담관세포군에서 AE2 유전자의 발현은 대조군인 정상 담관세포군에 비해서 낮았다. 결찰 담관세포군에서 AE2 유전자의 발현은 결찰 기간에 따라 차이가 없었다. 담관 결찰 간세포군에서 AE2 유전자의 발현은 대조군인 정상 간세포군에 비해서 경계적 유의성을 보이며 증가하는 경향이 있었다. 결찰 간세포군에서 AE2 유전자의 발현은 결찰 기간에 따라 차이는 없었다. 따라서 CFTR AE1 AE2 그리고AE3 는 간세포와 담관 세포에서 중탄산염이온과 수액을 매개하는 주된 이온 전달체라는 사실을 고려할 때 담도 담즙정체 간질환에서CFTR과 AE2 발현의 변화는 병리학적 기전에 중요한 역할을 할 수 있으리라고 생각된다. Cystic fibrosis transmembrane conductance regulator (CFTR) gene and sodium-independent Cl?/HCO₃? anion exchanger (AE) genes are expressed in a wide variety of mammalian tissues including cholangiocytes. They play an important role in the regulation of intracellular pH (pHi) as well as in transepithelial acid/base transport necessary for biliary bicarbonate secretion. The aim of this study was to examine the expression level of CFTR gene and AE genes (AE1, AE2 and AE3) in the cholangiocytes and the hepatocytes, and also measure AE2 gene expression level after bile duct ligation (BDL). As we previously described, isolated hepatocytes and cholangiocytes from the liver of normal and BDL rats were prepared and gene expression levels were measured by using RT-PCR. We found that AE1, AE2, and AE3 genes were expressed in both hepatocytes and cholangiocytes, but CFTR was only in cholangiocytes. AE2 gene expression level was higher in the BDL hepatocytes than normal hepatocytes, which was significantly different between two groups. AE2 gene expression level was lower in the BDL cholangiocytes than normal cholangiocytes. However, AE2 gene expression level in both hepatocytes and cholangiocytes were not changed with a longer duration of BDL. These results suggest that CFTR and AE2 may play an important role in the pathogenetic mechanism of biliary cholestatic liver disease.
내시경적 역행성 췌담관조영술중 췌선방 조영 후 발생한 중증 췌장염 1예
장세중,기승석,김훈일,신혜영,박장원,송은훈,태재웅 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2
내시경적 역행성 담췌관조영술 후 발생한 급성췌장염의 발생인자로 여러 가지 인자들이 관여하지만 특히 담췌관조영술 시행 후 과도한 췌선방 조영화에 의한 중증의 췌장염 증례를 경험하였기에 보고하는 바이다 Endoscopic retrograde cholangiopancreatography (ERCP) has been considered as a valuable tool for the diagnosis and management of disease of the pancreas and biliary tree. Post-ERCP pancreatitis is the most frequent and clinically significant complication following ERCP. But a few complication associated with this procedure are unforeseeable. Specially, acinarization as a result of a careless cooperation between operator and assistant may be influenced in complications of ERCP. Severe post-ERCP pancreatitis is rare complication, and its prognosis will be poor. Therefore, careful process and good relationship between operator and assistant in ERCP may be prevented this complication, but is not clear. Neglect of acinarization may be resulted in more aggravation of pancreatitis. We have cared for one patient with severe post-ERCP pancreatitis with pseudocyst after water-soluble contrast media spreading into the acini (acinarization) via ERCP in resolving phase idiopathic pancreatitis.
Diethylnitrosamine으로 유발된 흰쥐 간암 발생 및 진행 과정에서 VEGF와 Factor Ⅷ R-Ag의 발현
김형준,기승석,박용진,김남돈,견종만,박언섭 중앙대학교 의과대학 의과학연구소 2003 中央醫大誌 Vol.28 No.2-3
It is widely believed that chemical carcinogenesis is a multistage process, so-called initiation, promotion and propagation. Diethylnitrosamine (DEN) has been proved to have carcinogenic potential either at initiation or at promotion stage, resulting hepatocellular carcinomas in rat liver. In addition, neoplastic and metastatic propagation of transformed cells exclusively need a plenty of vascular supply for their metabolic requirement. To elucidate the tumor angiogenesis during the chemically induced carcinogenesis in rat liver, analysis of histopathology and the expression of vascular endothelial growth factor (VEGF) and Factor VⅢ related antigen were examined by immunohistochemical study using avidine-biotin complex. The hyperplastic nodules appeared at 3 weeks, the hepatocellular carcinomas were 15 weeks, and the pulmonary metastatic foci were developed at 18 weeks after oral administration of 120 ppm DEN to the rat. The VEGF expression appeared at 3 weeks and continuously increased with weeks. The staining of Factor VⅢ R-Ag was negative in any sinusoids in normal control livers, focal or scattered expressed in hyperplastic nodules, and diffusely expressed in hepatocellular carcinomas. As above results, it could be suggested that VEGF expression plays an important role from early period of DEN-induced hepatocarcinogenesis. Also, the expression of VEGF is likely to be associated with phenotypic changes of the sinusoidal endothelium presenting Factor VⅢ expression. However, there was no correlation between the expression pattern of VEGF and the state of metastasis.
N-butyl-N(4-hydroxybutyl) Nitrosamine에 의한 흰쥐방광암 발생과정에서 E-cadherin과 Catenin의 발현
박용진,기승석,김남돈,김성곤,박언섭 중앙대학교 의과대학 의과학연구소 2003 中央醫大誌 Vol.28 No.2-3
Although urothelial cell carcinoma is the most common primary tumor of the urinary bladder, the mechanisms that regulate its development and progression remain unclear. β-catenin plays a fundamental role in the regulation of E-cadherin-catenin cell adhesion complex. Mutations in either β-catenin or E-cadherin gene result in up or down-regulation of protein expression. This study was conducted to evaluate the expression changes of β-catenin and E-cadherin protein during 0.05% BBN-induced rat bladder carcinogenesis. Spray-Dawley Rats were given 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) by drinking water, and sacrificed at 5, 10, 20, and 30 weeks following treatment. For sequential changes and the expression of E-cadherin and β-catenin during rat bladder tumorigenesis, morphologic assesment and immunohistochemical staining were done. During the carcinogenesis, sequential histological changes from hyperplasia to papilloma, and ultimately overt carcinomas were noted. On histopathological findings, urothelial cell hyperplasias was appeared at 5 weeks, followed by urothelial papillomas at 10 weeks, and superficial urothelial carcinomas was found at 20 weeks after BBN administration. After 30 weeks of BBN administration, invasive carcinomas were developed. The immunohistochemical stains for E-cadherin and β-catenin displayed reduced expression in papilloma and carcinomas. Abnormal cytoplasmic expression of E-cadherin and β-catenin were observed in papillomas and carcinoms, especially in invasive carcinomas. In summary, the loss or reduced expression of E-cadherin and β-catenin are closely related with tumor propagation. Abnormal cytoplasmic expression of E-cadherin and β-catenin are late events favoring tumor progression in superficial type to invasive form in rat BBN-induced bladder cancer. The present study demonstrates that E-cadherin and β-catenin may play an important role in bladder carcinogenesis.
A Case of Composite (Mixed) denocarcinoma-Small Cell Carcinoma of the Stomach
Kim, Nam Don,Park, Yeon Hee,Ki, Seung Seog,Park, Yong Jin,Kim, Heoyng Joon,Ryoo, Baek-Yeol,Kim, Heung Tae,Chung, Jin-Haeng,Yuh, Young Jin 인제대학교 백병원 2003 仁濟醫學 Vol.24 No.1
A composite (mixed) tumor is defined as a glandular-endocrine cell carcinomas characterized by an intricate admixture of both elements in the same lesion. The histogenesis of this tumor is not clear but is considered to be multidirectional differentiation from pluripotential stem cells. The clinical findings are local symptoms due to mass and systemic symptoms due to paraneoplastic syndrome. The prognosis is considered to be very poor because of a high incidence of vasculo-lymphatic invasion and markedly deep infiltration of small cell carcinoma (SCC) of this composite tumor. Treatment has not been established clearly yet, but treatment with regimens specific for SCC of the lung may be used because the biologic and clinical characteristics of gastric SCC are similar to those of SCC of the lung. We encountered a 70-year-old man with pain to the back and both elbows for 20 days on July 22, 2002. Esophagogastroduodenoscopic biopsy revealed a superficially well differentiated adenocarcinoma and a deeply located. poorly differentiated SCC in the same lesion of the stomach, along with multiple bony metastases. We treated the patient with a chemotherapeutic regimen (etoposide and carboplatin) and palliative radiotherapy. The patient complained of sustained abdominal pain and poor oral intake and he died about 5 months later.
뼈에 발생한 악성 림프종 환자의 치료 효과 판정에서 ^(18)F-FDG Positron Emission Tomography의 유용성
김남돈,박연희,기승석,박용진,김형준,류백렬,김흥태,김성은,천기정,최창운,임상무 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.5
원발성골림프종은 매우 드문 질환이기는 하나, 복합항암요법이나 방사선 치료등에 높은 반응율을 보이며, 좋은 예후를 가지는 림프절의 비호지킨 림프종의 일종이다. 그러나, 치료 효과 판정에 있어 골병변의 특이성에 의한 모호한 방사선학적 특징으로 어려움이 있어 왔다. 본 증례는 위와 같은 원발성골림프종 환자에서 PET 스캔을 사용하여 민감하게 완전 반응을 평가할 수 있었던 예로, 앞으로 다른 고식적인 방법과 함께 이 질환의 진단과 치료 평가 있어 유용하게 사용 될 수 있을 것으로 전망할 수 있었다. Purpose: Accurate assessment of the lesion after treatment of patients with bone lymphoma is difficult. In this patient who demonstrated complete remission after chemotherapy, the regions of fluorine-18 fluorodeoxyglucose (^(18)FFDG)PET uptake diminished more rapidly following therapy, indicating a complete response at much earlier stage than did Magnetic Resonance Imaging (MRI) or CT based findings. With the conventional methods, such as MRI and CT. It was difficult to assess whether the residual tumor tissue was viable or not. Decision to complete response is very important in patients with ymphoma to plan the further treatment. We experienced a patient with primary lymphoma of bone who revealed complete response to chemotherapy on ^(18)FFDGPET while CT showed persistent destructive bone lesion. Thus, ^(18)FFDGPET study after therapy may be superior to CT in the evaluation of response to treatment in primary lymphoma of bone.