http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
구정현,장현영,이영지,문영재,배은주,윤석권,박병현 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
We recently reported that myeloid cell-expressed sirtuin 6 (Sirt6) plays a crucial role in M1 macrophage polarization and chemotaxis. Given the prominent role of macrophages during wound repair and macrophage heterogeneity, we hypothesized that a Sirt6 deficiency in myeloid cells would delay skin wound closure by affecting the phenotypes of macrophages in wounds. To address this question, a full-thickness excisional lesion was made in the dorsal skin of myeloid cell-specific Sirt6 knockout (KO) and wild-type mice. Wound closure was delayed in the KO mice, which exhibited less collagen deposition, suppressed angiogenesis, and reduced expression of wound healing-related genes compared to the wild-type mice. Using immunohistochemical, flow cytometric, and gene-expression analyses of macrophage subpopulations from wound tissue, we identified increased infiltration of M1 macrophages with a concomitant decrease in M2 macrophage numbers in the KO mice compared to the wild-type mice. Consistent with the in vivo wound closure defects observed in the KO mice, keratinocytes and fibroblasts treated with KO macrophage-derived conditioned medium migrated slower than those treated with wild-type macrophage-derived conditioned medium. An analysis of downstream signaling pathways indicated that impaired Akt signaling underlies the decreased M2 phenotypic switching in KO mice. These results suggest that a macrophage phenotypic switch induced by Sirt6 deficiency contributes to impaired wound healing in mice.
Effect of xanthohumol on melanogenesis in B16 melanomacells
구정현,김형태,Ha-Yong Yoon,권강범,최일환,정성후,김한욱,박병현,박진우 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.3
Xanthohumol (XH), the principal prenylflavonoid of the hop plant (Humulus lupulus L.), dose-dependently inhibited isobutylmethylxanthine (IBMX)-induced melanogenesis in B16 melanoma cells, with little cytotoxicity at the effective concentrations. Decreased melanin content was accompanied by reduced tyrosinase enzyme activity, protein and mRNA expression. The levels of tyrosinase-related protein 1 and 2 mRNAs were decreased by XH. XH also inhibited α-melanocyte stimulating hormone- or forskolin-induced increases in melanogenesis, suggesting an action on the cAMP-dependent melanogenic pathway. XH downregulated the protein and mRNA expression of microphthalmia- associated transcription factor (MITF), a master transcriptional regulator of key melanogenic enzymes. These results suggest that XH might act as a hypo-pigmenting agent through the downregulation of MITF in the cAMP-dependent melanogenic pathway.
구정현(Koo Jeong Hyun) 한국지역개발학회 2013 韓國地域開發學會誌 Vol.25 No.5
The purpose of this study is to understand the population and employment decentralization and the urban spatial structure of Incheon, focusing on the land use LQ by administrative diistrict 'Dong'. This study will help to suggest urban policy alternatives for inducing desirable urban spatial structure of Incheon. For this purpose, firstly the process of population and employment decentralization is analyzed through population and employment density distribution by Dong. Second, the land use LQ by Dong is calculated, using the land uses from building management registration, which are categorized into urban uses and non-urban uses. Third, The influence of the population and employment decentralization to the urban spatial structure of Incheon by regression analysis. The results of regression analysis are as follows. Firstly, population and employment decentralization is highly correlated process. Secondly. population service industry employment influence land use LQ in the opposite directions. Thirdly, sales and residential are the most prominent land uses in the process of population and employment decentralization and the change of the urban spatial structure of Incheon
Protease Activated Receptor-2의 길항제로서 Lobaric Acid의 피부 색소침착 억제 효능 연구
구정현 ( Jung Hyun Goo ),이지은 ( Ji Eun Lee ),명철환 ( Cheol Hwan Myung ),박종일 ( Jong Il Park ),황재성 ( Jae Sung Hwang ) 대한화장품학회 2015 대한화장품학회지 Vol.41 No.3
멜라노사이트에서 생성된 멜라노좀은 수상돌기를 따라 케라티노사이트로 이동한다. 세포막을 통한 정보 전달계에 관여하는 protease activated receptor-2 (PAR-2)는 SLIGKV와 같은 펩타이드에 의해 활성화되어 멜라노좀 전달을 증가하는 역할을 한다고 보고되어 있다. 본 연구에서는 새로운 PAR-2의 저해제를 찾고 본 저해제가 멜라노좀의 이동과 색소침착을 저해함을 확인하고자 하였다. PAR-2가 활성화되면 G 단백질이 방출되고, 이때 증가하는 세포 내 칼슘 이온 농도가 lobaric acid에 의하여 감소하는 것을 확인하여 lobaric acid가 PAR-2의 길항제로 작용할 수 있음을 발견하였다. 각질형성세포에서 SLIGKV에 의해 증가된 형광 비드 uptake가 lobaric acid에 의해 억제 되는 것을 확인하였고 또한, 분리된 멜라노좀을 이용한 시험에서도 동일한 경향을 나타내었다. 멜라노사이트와 케라티노사이트를 공동 배양하여 멜라노좀의 이동을 공초점 현미경으로 관찰한 결과, lobaric acid에 의해 멜라노좀의 전달이 억제되었다. 인공피부조직에 lobaric acid를 처리하였을 때 색소 침착이 억제됨을 확인하였고, 또한 Fontana-Masson 염색을 통해 멜라닌의 양이 감소함을 확인하였다. 이상의 결과를 통해 lobaric acid가 PAR-2 길항제로 작용함으로써 케라티노사이트로 멜라노좀 전달을 억제하고 이를 통해 피부 색소 침착을 저해함을 확인할 수 있었다. Melanosome, the pigment granule in melanocyte, determines the color of skin when it moves into the keratinocyte. Inhibition of melanosome transfer from melanocyte to keratinocyte results in skin depigmentation. Protease activated receptor-2 (PAR-2) is involved in signal transduction systems via cell membrane and increases the melasome transfer when it is activated by cleavage of their extracellular amino acid sequence by trypsin or by a peptide such as SLIGKV. Here, we showed that lobaric acid inhibited PAR-2 activation and affected the mobilization of Ca<sup>2+</sup>. The uptake of fluorescent microspheres and isolated melanosomes from melan-a melanocytes to keratinocytes induced by SLIGKV were inhibited by lobaric acid. Also, confocal microscopy studies illustrated a decreased melanosome transfer to keratinocytes in melanocyte-keratinocyte co-culture system by lobaric acid. In addition, lobaric acid induced visible skin lightening effect in human skin tissue culture model, melanoderm<sup>Ⓡ</sup>. Our data suggest that lobaric acid could be an effective skin lightening agent that works via regulation of phagocytic activity of keratinocytes.
장현영,구정현,이상명,박병현 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Previous clinical trials have addressed the beneficial effects of fish oil supplementation on atopic dermatitis. Recently, we reported that fat-1 mice, which can convert n-6 to n-3 polyunsaturated fatty acids (PUFAs), are protected against allergic airway inflammation because their Th2 immune responses are suppressed. Here, we examined the effects of endogenously synthesized n-3 PUFAs on atopic dermatitis, a representative Th2-dominant allergic inflammatory disease. Mouse models of atopic dermatitis-like skin lesions were prepared by epicutaneous application of 2,4- dinitrochlorobenzene (DNCB) or house dust mite (HDM) extract to the ears. DNCB-treated fat-1 mice exhibited markedly reduced epidermal thickening, lower mast cell infiltration, and lower serum IgE and histamine compared with wild-type mice. The draining lymph nodes of fat-1 mice were substantially smaller and contained significantly smaller proportions of activated CD4+ T cells and IL-4-producing Th2 cells than those of wild-type mice. Consistent with these findings, the mRNA levels of Th2 cytokines were significantly decreased in DNCB-sensitized skin lesions of fat-1 mice. Lastly, inflammasome activation, IL-1β production, and pyroptotic cell injury were suppressed in fat-1 mice. Similar results were observed in HDM-challenged fat-1 mice. This study confirms the results of previous clinical studies and suggests fish oil supplementation as a therapeutic strategy for atopic dermatitis-like skin lesions.