남성생식세포 Sertoli cell에 미치는 복분자(覆盆子)의 항산화 효과

김영주,장문석,박성규,Kim, Young Joo,Chang, Mun Seog,Park, Seong Kyu 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.2

Objectives : The purpose of this study was to examine the antioxidant effects of the extract of Rubi Fructus on TM4 Sertoli cells. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity and cell viability assays on Sertoli cells. In addition, hydrogen peroxide-induced oxidative stress on Sertoli cells were examined by MTT assay. The antioxidant enzyme of Cu/Zn SOD, Mn SOD, catalase protein expression on Sertoli cells were also measured. Results : The results showed that the extract scavenged DPPH radical dose-dependent manner. The extract showed no cytotoxicity at concentration of 1, 5, 10, 50, $100{\mu}g/ml$. The hydrogen peroxide-induced cytotoxicity of Sertoli cells was protected to 88.3% by the extract at concentration of $100{\mu}g/ml$. Cu/Zn SOD and Mn SOD protein expression were significantly increased on Sertoli cells, but catalase protein expression was not significantly changed. Conclusions : In conclusion, the extract of Rubi Fructus has antioxidant effects on Sertoli cells and protect male reproductive system against oxidative stress.

주거복지에 대한 사회복지업무 종사자의 의식조사 연구

김영주,김미희,박남희,Kim, Young-Joo,Kim, Mi-Hee,Park, Nam-Hee 한국주거학회 2010 한국주거학회 논문집 Vol.21 No.6

The purpose of this study was to identify perception on housing welfare of social welfare practitioners. Specific research interest was focused on the area of housing welfare services and necessity, problems when execute housing welfare services, and activating strategy of housing welfare. For the research purpose, questionnaires were distributed to 537 people who work in the field of social welfare including social welfare officials in local government authorities and social workers at community welfare centers nationwide by mail and e-mail and 405 questionnaires were analyzed using SPSS 15.0 program. Respondents thought that the supply of public rental housing was the most important service to promote housing welfare. They also indicated the most serious problem was the lack of budget and housing welfare services and programs. As main reasons of local government's passive attitude toward housing welfare services and programs, respondents mentioned low interest level of housing welfare and lack of housing welfare education. Most of the respondents agreed the necessity of introducing the curricula related to housing welfare and expected to spread interest toward housing welfare in the field of social welfare.

공공임대주택 주민의 생활관리업무 참여에 대한 관리자 의식조사

김영주,이진영,Kim, Young-Joo,Lee, Jin-Young 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.6

Resident participation or tenant empowerment is becoming an important concept of planning, provision of welfare services, and management of housing. It can be motivated by three variables: shared goals, shared values, and sense of community among residents. Theoretically, the more each of these three variables, the more likely people will be to participate. The purpose of this study was to identify the perception of management staff on residents' participation in public rental housing. For this, 198 management staff of public rental housing under Korea Housing Management were surveyed using questionnaire distributed by email during February 18 to 27, 2009. Only 33.3% of the public rental housing complexes surveyed constituted Residents Representative Committee. Most of the respondents showed negative perception of resident organizations and their roles as an meaningful way to communicate with them on the management system. Several ways to enhance the resident participation and to reduce housing and living cost burden of residents in public rental housing were suggested as follows: development and distribution of education and service program inspiring the residents' empowerment, enacting a provision of Tenants Representative obligation in Rental Housing Act, and so on.

분유에 오염된 Cronobacter sakazakii 검출을 위한 중합효소연쇄반응, 실시간중합효소연쇄반응, 등온검출법의 비교

김영주,서승우,왕효우,서동주,이민화,손나리,이복희,최창순,Kim, Young-Joo,Seo, Sheungwoo,Wang, Xiaoyu,Seo, Dong Joo,Lee, Min Hwa,Son, Na Ry,Lee, Bog-Hieu,Choi, Changsun 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.5

Loop-mediated isothermal amplification (LAMP) is an emerging detection technology for the amplification of DNA under isothermal conditions. The aim of this study was to develop a rapid and reliable LAMP technique for the detection of Cronobacter sakazakii in milk powder. In order to enhance the sensitivity and specificity, LAMP primers targeting outer membrane protein A (ompA) gene of C. sakazakii were designed using Explorer V4 software. Thirty seven C. sakazakii strains and 13 pathogenic microorganisms were used for comparative detection of C. sakazakii using polymerase chain reaction (PCR), real-time PCR, and LAMP. LAMP developed in this study could specifically detect C. sakazakii strains without cross-reactivity with other foodborne pathogens. LAMP products amplified from ompA gene of C. sakazakii were digested with with HhaI and NruI enzyme. The specificity of LAMP was confirmed by restriction fragment length polymorphism (RFLP) analysis. LAMP could detect C. sakazakii within 1 h without bacterial culture and its detection limit was as low as 1 CFU/mL C. sakazakii in milk. In the comparison of the sensitivity, LAMP showed 10,000- and 100-times higher detection limit than PCR or real-time PCR, respectively. Therefore, this study can conclude that LAMP is a rapid and reliable detection technique for C. sakazakii contaminated in powdered milk. 본 연구에서는 영유아에게 치명적인 감염을 일으키는 C. sakazakii에 대하여 LAMP 검출법을 개발하였다. LAMP법에 의한 C. sakazakii의 검출율은 100%였으며 13개의 음성 지표군에 대해서는 모두 음성 반응을 보여 특이도가 매우 높은 것으로 판단되었다. 또한, HhaI과 NruI 두 개의 제한 효소를 LAMP product에 반응시킨 결과, 유전자의 특정 염기서열이 절단되는 것을 확인하였으며, 이를 통해 LAMP 검출법에 의해 증폭된 DNA가 C. sakazakii-specific ompA임을 확인하였다. 조제분유에 오염 된 C. sakazakii를 LAMP법으로 검출 시 검출한계는 $10^0$ CFU/mL이었으며 이는 기존의 PCR법이나 real-time PCR법에 비해 100-10,000배 높은 수준으로 민감도가 매우 높은 것으로 판단되었다. 이와 같이 높은 특이도와 민감도를 가진 LAMP 검출법은 C. sakazakii와 같은 급성 기회 감염균이나 병원성 미생물에 의한 식중독 발생시 현장에서 병원체를 간편하고 신속하게 검출할 수 있는 기술로 기대된다.

영구임대주택 입주자의 관리비 및 임대료 체납 실태와 의식

김영주,김영태,Kim,, Young-Joo,Kim, Young-Tae 한국주거학회 2007 한국주거학회 논문집 Vol.18 No.5

Permanent rental housing, the construction of which was strongly financed by the central government, is regarded as a social housing for those who fall within the lowest income bracket. Differing from "public rental housing", offered to tenants for sale after a five year rental period, this type of housing is intended for rental use on a long-tenn basis. At present, about 190,000 permanent rental housing units exist in Korea. According to a statistics, 15.6% of its residents did not pay their management fee and rental payment in 4 or more months in 2005, which places stress on the housing management. Based on the "eviction condition" stipulated in the tenancy agreement for permanent rental housing, a householder owning assets or a vehicle which is not used as his means of living, who is overdue with his management fee and rental payment for a long time, may be evicted from the house. However, there are many conflicts and problems between administrators/housing managers and residents in the process of enforcing this regulation. The purpose of this study is to explore the key issues associated with the present situation and the reasons why so many management fee and rental payment for permanent rental housing are overdue. For the purpose of research, data were collected from 10,990 permanent rental housing residents nationwide via a questionnaire survey in February 2007. One third of the respondents had an experience of more than 2 months overdue since they have moved in current residence. For further analysis, the respondents were divided into three groups, based on their working ability. The major finding showed that the group of respondents who have working ability required a more practical plan, such as employment, to have a sustainable life, while the other group of no labor force indicated a need for more housing allowance from the government. To suggest more specific alternatives for the subject of housing payment overdue, further comparison study should be performed between the residents of permanent rental housing and other low income tenants in private housing sector.

영구임대주택 관리업무 및 운영실태에 대한 관리자와 입주민의 의식비교

김영주,Kim, Young-Joo 한국주거학회 2007 한국주거학회 논문집 Vol.18 No.5

In recent, the concept of management is emphasizing the aspects of social welfare, especially in permanent rental housing that most of the residents are very low income households. Although the quality of service for the residents of permanent rental housing was improved comparatively, it still needs to continually change in response to the needs of residents. To figure out the better way of supporting the service for the residents of permanent rental housing, this study examined the role and function of management office and compared perception on management services between management staffs and residents of permanent rental housing. Based on the in-depth interview with 5 managers of permanent rental housing, two types of survey questionnaire (residents/staffs of management office) were developed for the research purpose. A sample of 89 management office staffs and 111 residents were used for the data analysis. Most of the respondents recognized the need of a cooperation system among management office, social welfare center, and residents for providing better management system and welfare service.

FMM 기법의 산란해석 정확도 향상 연구

김영주,조영기,손현,Kim, Young-joo,Cho, Young-Ki,Son, Hyun 한국전자파학회 2001 한국전자파학회논문지 Vol.12 No.6

FMM(Fast Multipole Method)은 계산시간을 현저히 감소시킬 수 있기 때문에 대형구조의 레이다 반사면적 (RCS) 계산에 매우 효과적인 수치해석 기법이다. 하지만, 폭이 좁은 타원형 형태의 산란체는 절점의 길이나 그룹의 선택방법이 수치해석 결과에 영향을 미치게 된다. 본 논문에서는 TM 및 TE 편파에 대해 이러한 문제를 효과적으로 처리할 수 있는 방법을 제안하고, 제안한 방법을 검증하기 위하여 MOM 결과와 비교하여 제시하였다. FMM(Fast Multipole Method) is suitable numerical method for radar cross section calculation of arbitrary large conducting bodies due to reduction of computation time. The accuracy of the numerical results, however, can influenced by selection of grouping method and segment length, in particular, far the case that cross section of the scatter is of the narrow width elliptical type. So, we describe the FMM method which can be deal effectively with such difficulties for both TM and TE polarization case. In order to check the present method the results are compared with those obtained by Method of Moments.

2차원 Open Cylindrical Cavity의 전자파 투과 및 산란특성연구

김영주,조영기,Kim, Young-Joo,Cho, Young-Ki 대한전자공학회 2001 電子工學會論文誌-TC (Telecommunications) Vol.38 No.11

2차원 open cylindrical cavity의 전자파 투과 및 산란특성에 대해 연구하였다. 이러한 구조는 cavity 및 aperture의 크기에 따라 전파의 투과나 산란특성에 특이한 현상이 나타남에도 불구하고 정확한 분석이 이루어지지 않았다. 본 논문에서는 모멘트법의 확장형인 FMM 기법을 이용하여 비교적 광범위하게 open cavity의 특성을 계산하였다. 계산결과 open cylindrical cavity의 경우 closed cavity의 internal mode에 대응하는 external mode가 존재하고, 이때 공진 및 산란특성은 비공진시와 다른 특성을 나타냄을 확인하였다. 연구결과는 전자파의 차폐 및 RCS 통제분야에 응용할 예정이다. Field penetration and scattering characteristics of two dimensional open cylindrical cavity is studied. Exact analysis for this sort of structure is not achieved even if there are unusual phenomena of field penetration and scattering with cavity and aperture size. In this paper, we calculate a wide range of open cavity characteristics by using of FMM method, which is extended method of MOM. We find external mode of open cylindrical cavity corresponding to internal mode of closed cavity. The characteristics of resonance and scattering of this region is different compare with non resonant area. The result of study will apply to the EM wave shielding and RCS control.

임베디드 디바이스에 적용 가능한 부분학습 기반의 실시간 손글씨 인식기

김영주,김태호,Kim, Young-Joo,Kim, Taeho 한국정보통신학회 2020 한국정보통신학회논문지 Vol.24 No.5

딥러닝 기술은 실세계의 객체를 분류하거나 인식하기 위해서 사용된다. 이를 위해서 준비된 많은 데이터를 고성능 컴퓨터에서 학습한 후에, 그 학습모델을 인식기에 탑재하여 각종 객체들을 인식한다. 이러한 인식기는 다양한 환경에서 사용되면서 인식하지 못하는 객체들이나 인식률이 낮은 객체들이 발생할 수 있다. 이런 문제를 해결하기 위해서 실세계 객체들을 주기적으로 학습하여 인식률을 높인다. 하지만, 즉각적인 인식률 향상이 어려울 뿐만 아니라, 임베디드 디바이스 등에 탑재되어 있는 인식기에서 학습하는 것이 쉽지 않다. 따라서, 본 논문에서는 임베디드 디바이스에 적용 가능한 부분 학습 기반의 실시간 손글씨 인식기를 제안한다. 제안된 인식기는 사용자 요청 시마다 임베디드 디바이스에서 부분 학습을 할 수 있는 환경을 제공하고, 실시간으로 인식기의 학습모델이 갱신된다. 이로 인해서 인식기의 지능이 지속적으로 향상됨으로 최초에 인식하지 못했던 손글씨에 대해 인식이 가능해진다. 이렇게 제안된 인식기는 RK3399 임베디드 디바이스에서 22개의 숫자와 글자에 대해서 학습과 추론이 가능하다는 것을 실험을 통하여 사람 손으로 쓴 은행 계좌명과 계좌번호를 인식할 수 있는 개인화된 지능을 가진 스마트 기기에 활용 가능할 것으로 기대된다. Deep learning is widely utilized to classify or recognize objects of real-world. An abundance of data is trained on high-performance computers and a trained model is generated, and then the model is loaded in an inferencer. The inferencer is used in various environments, so that it may cause unrecognized objects or low-accuracy objects. To solve this problem, real-world objects are collected and they are trained periodically. However, not only is it difficult to immediately improve the recognition rate, but is not easy to learn an inferencer on embedded devices. We propose a real-time handwriting recognizer based on partial learning on embedded devices. The recognizer provides a training environment which partially learn on embedded devices at every user request, and its trained model is updated in real time. As this can improve intelligence of the recognizer automatically, recognition rate of unrecognized handwriting increases. We experimentally prove that learning and reasoning are possible for 22 numbers and letters on RK3399 devices.

금속친화 크로마토그래피를 이용한 단백질분리의 특성연구

김영주,김승재,Steven M . Cramer ( Young Joo Kim,Seung Jai Kim,Steven M . Cramer ) 한국화학공학회 1995 화학공학의이론과응용 Vol.1 No.2