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      • SCOPUSKCI등재

        제주마(濟州馬)의 위장관(胃腸管)에 기생(寄生)하는 Gasterophilus spp의 분류(分類) 및 분포조사(分布調査)

        김승호,Kim, Seung-ho 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

        This paper dealt with the situation and the hatch rate of bot's eggs on the equine hairs in Cheju horse with the species grouping of the bot flies of equine(genus Gasterophilus). The prevalence and infection dynamics of Gasterophilus spp. larvae was also evaluated in the equine alimentary canal submitted in this laboratory for the necropsy or from the abattoir. Samples including Gasterophilus spp. larvae, bot's flies and its eggs, which were collected from the alimentary canal and equine hairs, respectively, were studied and classified by morphology. The morphologic feature of the spines of Gasterophilus larvae were studied by scanning electron microscope. 1. Gasterophilus intestinalis larvae concentrated in the nonglandular portions of the stomach. The infection of second-, and third stage larvae were common in November, and from January to October, respectively. Gasterophilus nasalis larvae were commonly identified on the gastric pylorus and upper portion of duodenum. Second stage larvae were found from October to December, and 3rd stage larvae, from January to September. 2. The hatch rate of laid eggs of Gasterophilus intestinalis was 28.4%, and that of Gasterophilus nasalis was 79.5%. The hatch rate of Gasterophilus intestinalis eggs was highest(62.5%) in December. The hatch rate of laid eggs were higher in the region of scapula(64.0%) and limbs(62.5%) than on the maned hairs. The eggs of Gasterophilus nasalis were completely hatched by October. 3. Eight hundred five Cheju horses examined in this study were infected with the eggs of Gasterophilus spp. Gasterophilus intestinalis eggs on the body regions from sixty horses were recognized in phalangeal (14.4%), in abdominal(13.8%), metacarpal, brachial and cervical regions. Gasterophilus nasalis eggs were uncommon and recognized in submandibular regions(1.4%). 4. In conclusion, the infection of imago, larvae and eggs of both Gasterophilus intestinalis and Gasterophilus nasalis were indentified in cheju horse.

      • SCOPUSKCI등재

        된장으로부터 혈전용해능을 가진 Bacillus sp.의 분리 및 동정

        김승호,최낙식,이우일,이종우,김동호,Kim, Seung-Ho,Choi, Nack-Shick,Lee, Woo-Yiel,Lee, Jong-Woo,Kim, Dong-Ho 한국미생물학회 1998 미생물학회지 Vol.34 No.3

        우리 나라의 전통 대두발효식품인 된장으로부터 혈전용해능을 가진 미생물을 분리하였다. 그 중 5종류의 균주를 Bergey's manual of systematic bacteriology에 의거하여 동정한 결과 Bacillus sp. 균주로 밝혀졌다. 분리된 Bacillus 속 미생물을 효소 유도배지에서 배양한 결과 B. amyloliquefaciens는 2.46 plasmin unit/ml, B pantothenticus는 3.82 plasmin unit/ml 의 혈전용해능을 가지고 있었고, B. subtilis는 4.94 plasmin unti/ml의 높은 혈전용해효소 생산능을 보여주었다. 분리 균주에 의하여 생산된 세포외 단백질을 SDS-PAGE와 reverse fibrin zymogram 활성측정법에 의해 확인한 결과 각 균주별로 서로 다른 혈전용해효소가 생산되었음을 확인하였다. Various bacterial strains that secret extracellular fibrinolytic enzyme were screened from Doen-Jang, a traditional soybean fermented food in Korea. Five microbes of them were identified to be Bacillus sp. strains according to Bergey's manual of systematic bacteriology. The culture filtrates of B. amyloliquefaciens (2.46 plasmin unit/ml) and B. pantothenticus (3.82 plasmin unit/ml) showed a level of fibrinolytic activity that was about three times higher than that of plasmin 1.0 unit and Bacillus subtilis showed the highest fibrinolytic activity (4.94 plasmin unit/ml). All of the extracellular proteases showing the fibrinolytic activity are confirmed by SDS-PAGE followed by reverse fibrin zymogram activity assay and we proposed that some of the fibrinolytic enzymes from this work are novel enzymes.

      • SCOPUSKCI등재

        제주도에(濟州道) 있어서 Toxoplasma 항체분포(抗體分布)에 관(關)한 연구(硏究) 1. 돼지, 고양이 및 식육취급자(食肉取扱者)에 있어서의 Toxoplasma 항체분포(抗體分布)에 대하여

        김승호,김영주,Kim, Seung-ho,Kim, Young-ju 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.3

        Effect of raising types and environmental conditions on the infection of Toxoplasma in the swine, the cat and the man were studied in Cheju Island from Sept. 1987 to Aug. 1988. Blood samples were taken from 214 conventionally raised swine in 6 villages and 506 swine raised in swine specialized farms, 122 cats raised under free moving or restraned conditions in 8 locations, 113 butchers, and 210 villagers. Toxoplasma antibody values of the blood sera were determined using the enzymelinked immunosorbent assay (ELISA). The eating type of viscera was also investigated by using questionaires 1. When ELISA method was used, the percentage of Toxoplasma infected swine among the conventionally raised and of those raised in swine specialized farms were 60.7% and 21. 390, respectively. The respective mean of antibody values (${\pm}SD$) were 0.589 (${\pm}0.310$) and 0.385 (${\pm}0.237$) and differed very significantly (p<0.01). A significant difference was also found in antibody values among 6 villages (p<0.05). 2. The mean infection percentage of toxoplasma in the cat was 38.2%. the infection percentage for cats raised under free-moving and re~;trained condition were 37.0% and 38.2% respectively. The respective antibody values(${\pm}SD$) for toxoplasma were 0.600(${\pm}0.614$) and 0.637 (0.645), and did not difference significantly. 3. The infection percentage of toxoplasma in villagers and butchers were 26.2 and 38.3% respectively. The respective antibody values (SD) for toxoplasma were 0.429(${\pm}0.195$) and 0.341 (${\pm}0.236$), and differed very significantly (p<0.01). There were also highly significant differences Pyo-sun and other village (p<0.01). 4. Analysis of the questionaires showed that 26.0% of 392 villages eated liver and some villagers eated other viscera.

      • KCI등재

        동종 이식 반월상 연골의 시간적 변화 - 자기 공명 영상 , 육안적 , 조직학적 연구 -

        김승호,하권익,안진환,장동국 ( Seung Ho Kim,Kwon Ick Ha,Jin Hwan Ahn,Dong Kook Chang ) 대한슬관절학회 1998 대한슬관절학회지 Vol.10 No.1

        Vascular ingrowth is essential for the survial of the graft tissue. The purposes of this study were to evaluate any changes in signal intensity of cqropreserved meniscal allograft with time in the magnetic resonance images(MRI) and to demonstrate the viability of the grafts. Eight patients underwent meniscal transplantation with cryopreserved allcgrafts using the bone block technique. MRIs of the knee were taken at 3 days, 3 weeks, 6 weeks, 3 month, 6 months. And I year after the implantation. A second-look arthroscopy and a small meniscal biopsy(sized 2mm x 2mm1 at the peripheral and central part of the meniscus were conducted at 3 months and 1 year. Three days after the operation, the signal intensity of the implanted meniscus revealed a homogenrous low signaJ intensity that could not be differentiated from that of' a contralateral normal meniscus. At 3 weeks, a high signal intensity appeared at the periphery of the meniscus. This signal, which did not communicate into the joint space, fuither intensified at 6 weeks. The high signal intensity of the meniscus, though still higher than that of the normal meniscus, decrexsed slightly at 3 months and continued to decrease progressively even a year after the implantation. The second-look arthroscopy revealed that the grafts were viable and that there was no tearing or shrinking of the meniscus. Cellular proliferation was also found at the central edge oi' the meniscus at 3 months. This cellular pattern differentiated from that of a nonmal meniscus in that the d stribution of cells was not in an even, but in a clonal pattem. The cellularity after a year, however, was sirnilar to that of normal meniscus except some area with deficiency of cells. It can be concluded that increased signal intensity of' the implanted meniscus with time indicates hypervascularity caused by vascular ingrowth, similar to the high signal intensity ot>tained from normal meniscus in young children. Increasecl signal intensity in the chronological postoperative MRls demonstrates the viability of the implanted cryopreserved meniscal allograft.

      • 만성 족관절통 환자에서의 족관절 관절경 소견

        김승호,하권익,한계영,Kim, Seung-Ho,Ha, Kwon-Ick,Han, Kye-Young 대한관절경학회 1998 대한관절경학회지 Vol.2 No.2

        The purpose of this study is to evaluate the significance of arthroscopic examination in patients with chronic ankle pain. Eighty eight arthroscopic procedures were conducted due to symptoms of chronic ankle pain. The pathology of the 88 ankles could be categorized into four groups ; 22 anterior impingement syndrome, 20 anterolateal impingement syndrome, 22 instability and 20 osteochondral lesion, 59 of patients(67.0%) had trauma history and 14(15.9%) of patients had injured chronic repetitive microtrauma. During the arthroscopic procedure, we found unexpected chondral lesions of high incidence(39.1%). These lesions were not detected both by the physical examination and radiologic examinantion. These pathologies were treated during the course of arthroscopic procedure. We concluded that ankle arthroscopy may be a very useful and therapeutic tool in the patients who have not reponded to the conservative treatment. And due to high incidence of intra-articular pathology in patients with chronic ankle pain, arthroscopic examination should be performed prior to final decision.

      • KCI등재

        냉종 동종 이식 반월상연골의 자기공명영상 및 2차 관절경 소견 - 예비 보고 -

        김승호,하권익,윤경호 ( Seung Ho Kim,Kwon Ick Ha,Kyoung Ho Yoon ) 대한슬관절학회 1996 대한슬관절학회지 Vol.8 No.2

        Recent successful results of allograft meniscal transplantation are based on the many experimental and clinical irials. The purposes of this study are to evaluate the change of signal intensity of cryopreserved ineniscal allograft on magnetic resonance image and to demonstrate viability of the grafts. Three patients unclerwent meniscal transplantation with cryopreserved allograft and MRI of' the knee at 1 day, 3weeks, 6 weeks. 12 weeks and 6 rnonths after implantation. Tissue biopsy was done at 3 months. At immediate postoperative day, signal intensity of the implanted meniscus revealed homogenous low si nal intensity which cou]d not be differentiated from contralateral normal meniscus. At 3 weeks, high signal intensity was appeared at the periphery and it was increased at 6 weeks. The high signal intensity of the meniscus ivas decreased slightly at 12 weeks. Cellular proliferation was found at the central edge of the implanted meniscus at 3 rnonths. It can be concluclecl that in creased signal intensity at 3, 6 and 12 weeks represent vascular ingrowth and viability of the implanted cryoppreserved meniscal allograft.

      • KCI등재

        광주지역 산업단지 도장·인쇄시설의 휘발성유기화합물 배출 특성 평가

        김승호,서동주,김하람,박진환,이기원,배석진,송형명,Kim, Seung-Ho,Seo, Dong-Ju,Kim, Ha-Ram,Park, Jin-Hwan,Lee, Ki-Won,Bae, Seok-Jin,Song, Hyeong-Myeong 한국환경과학회 2020 한국환경과학회지 Vol.29 No.5

        This study analyses the characteristics of volatile organic compounds (VOCs) emissions from the painting and printing facilities, as well as ambient VOCs at industrial complexes in Gwangju. The major components of VOCs emissions from painting facilities were toluene, acetone, 2-butanone, ethyl acetate, ethyl benzene, o-xylene and m,p-xylene. The printing facilities mostly emitted ethyl acetate, 2-butanone, acetone and toluene. Aromatics (49.9%) and oxygenated VOCs (43.6%) were dominant in painting facilities, while oxygenated VOCs (92.7%) were the largest group in printing facilities. The total hydrocarbon concentration (THC) in printing facilities was approximately six times higher than in the painting facilities. The painting and printing facilities use many solvents. Their THC concentrations differed considerably depending on the type of prevention facilities. To reduce THC, it is necessary to improve the prevention facilities and operating conditions. The dominant species of ambient VOCs in industrial complexes were investigated with toluene, ethyl acetate, 2-butanone, ethyl benzene, m,p-xylene, butyl acetate, o-xylene, hexane and acetone. Factor analysis of ambient VOCs showed that the main sources of the VOCs were organic solvents used in painting, coating, and printing, as well as automobile emissions.

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