맨드라미(Celosia cristata L.) 꽃 메탄올 추출물로부터 용매분획된 분획물의 항산화활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

맨드라미꽃의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성에 대해 검토하였다. 맨드라미꽃의 항산화성분과 활성은 메탄올 추출물과 순차적 용매분획물에 대해 측정하였으며, 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 추출수율은 각각 23.33, 10.27, 20.00, 13.63, 17.55 및 38.54%였다. 맨드라미 꽃 메탄올 추출물의 총 polyphenol, flavonoid 및 tannin 함량은 각각 6.80 ㎎ GAE/g ME, 2.34 ㎎ CE/g ME 및 6.23 ㎎ TAE/g ME였다. 순차적 용매분획물 중 butanol 분획에서 각각 14.92 ㎎ GAE/g SF, 5.44 ㎎ CE/g SF 및 13.38 ㎎ TAE/g SF로 함량이 높았다. 총 proanthocyanidin 함량은 44.72 ㎍ CE/g ME였고 순차적 용매분획물은 각각 42.47, 44.43, 50.03, 49.12 및 41.80 ㎍ CE/g ER로 용매간 유의적인 차이를 보이지 않았다. 맨드라미꽃의 메탄올 추출물의 DPPH 및 ABTS radical 소거활성은 각각 5.24 및 10.70 ㎎ TE/g ME였고 butanol 분획에서 각각 12.53 및 21.09 ㎎ TE/g SF로 활성이 높았다. 메탄올 추출물의 SOD 유사활성은 7.96 unit/mL로 나타났고 hexane, chloroform, ethyl acetate, butanol 및 물 분획물은 각각 4.56, 6.15, 8.07, 12.36 및 5.21 unit/mL로 butanol 분획의 활성이 높았다. 맨드라미꽃 추출물의 항산화성분과 항산화활성 간에 높은 정의 상관관계를 보였고 맨드라미꽃 추출물이 체내 및 식품에서 유익한 도움을 줄 수 있을 것으로 생각된다. The purpose of this study was to evaluate the antioxidant contents and activities of solvent fractions from methanolic extracts of cockscome flowers. The yield of methanolic extracts from cockscome flowers was 23.33%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butanol, and water) were 10.27, 20.00, 13.63, 17.55, and 38.54%, respectively. Total polyphenolic, flavonoid, tannin, and proanthocyanidin contents of methanolic extracts (ME) were 6.80 ㎎ gallic acid equivalent (GAE)/g ME, 2.34 ㎎ catechin equivalent (CE)/g ME, 6.23 ㎎ tannic acid equivalent (TAE)/g ME, and 44.72 ㎍ CE/g ME, respectively. The highest total polyphenolic, flavonoid, and tannin contents of solvent fractions were 14.92 ㎎ GAE/g solvent fraction (SF), 5.44 ㎎ CE/g SF, and 13.38 ㎎ TAE/g SF in the butanol fraction, respectively. The total proanthocyanidin contents were 42.47, 44.43, 50.03, 49.12, and 41.80 ㎍ CE/g SF, respectively. The DPPH and ABTS radical scavenging activities from cockscome flowers were 5.24 and 10.70 ㎎ Trolox equivalent (TE)/g ME, respectively. The highest DPPH and ABTS radical scavenging activities of the solvent fractions were 12.53 and 21.09 ㎎ TE/g SF in the butanol fraction, respectively. SOD-like activities of methanolic extracts from cockscome flowers were 7.96 units/mL, whereas those of its solvent fractions were 4.56, 6.15, 8.07, 12.36, and 5.21 units/mL, respectively. The results of this study show that notable antioxidant activities in cockscome flowers have significant health benefits.

한국인 관상동맥의 변화양상에 대한 형태학적 연구

권태정,이상용,이한영,고영창,권기석,조재홍,김태공,김유훈,조갑래,서중석,이원태 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

비수리(Lespedeza cuneata G. Don) 메탄올 추출물로부터 분획된 용매분획물의 항산화활성과 α-Glucosidase 저해활성

김현영(Hyun Young Kim),고지연(Jee Yeon Ko),송석보(Seuk Bo Song),김정인(Jung In Kim),서혜인(Hye In Seo),이재생(Jae Saeng Lee),곽도연(Do Yeon Kwak),정태욱(Tae Wook Jung),김기영(Ki Young Kim),오인석(In Seok Oh),정헌상(Heon Sang Jeong),우 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11

비수리의 식품학적 이용가능성을 확인해 보고자 항산화성분 및 항산화활성, superoxide dismutase 유사활성, α-glucosidase 저해활성 등에 대해 검토하였다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획 등 순차적 용매분획물의 총 폴리페놀 함량은 각각 12.44, 3.61, 6.39, 27.11, 20.00 및 9.32 ㎎ GAE/g extract residue(ER)로 나타났으며, 총 플라보노이드 함량은 각각 2.94, 9.92, 7.77, 9.27, 5.11 및 2.66 ㎎ CE/g ER, 총 탄닌 함량은 각각 8.75, 10.04, 7.42, 17.32, 11.65 및 7.61 ㎎ TAE/g ER, 총 프로안토시아니딘의 함량은 346.09, 63.50, 103.76, 288.62, 231.99 및 358.48 ㎍ CE/g ER로 나타났다. 비수리 메탄올 추출물과 hexane, chloroform, ethyl acetate, butanol 및 물 분획물의 DPPH radical 소거활성은 각각 20.62, 5.16, 9.29, 20.80, 20.00 및 20.79 ㎎ TE/g ER, ABTS radical 소거활성은 각각 33.86, 9.24, 17.36, 33.76, 33.49 및 33.86 ㎎ TE/g ER로 나타났다. SOD 유사활성은 각각 4.12, 0.61, 2.01, 9.89, 13.47 및 11.82 unit/mL의 활성을 보여 butanol 분획과 물 분획에서 유의적으로 높은 활성을 나타내었고 α-glucosidase 저해활성은 물 분획 50 및 25 ㎍/mL에서 각각 93.85 및 61.64%로 높은 저해활성을 보이는 것으로 나타났다. 이상의 결과에서 비수리 추출물은 항산화성분 및 항산화활성, SOD 유사활성, α-glucosidase 저해활성을 가지는 물질을 함유한 것으로 보이며, 체내 및 식품에서 활성산소 종 제거에 도움을 줄 수 있을 것으로 보인다. The purpose of this study was to evaluate the antioxidant contents and antioxidant activities of solvent fractions from methanolic extract of sericea lespedeza. To determine the antioxidant compounds in solvent fractions from methanolic extract, total polyphenolic, flavonoid, tannin, and proanthocyanidin contents were measured by spectrophotometric methods. Solvent fractions were evaluated for antioxidative capacity according to DPPH and ABTS radical scavenging activities. Total polyphenolic contents were 12.44, 3.61, 6.39, 27.11, 20.00, and 9.32 ㎎ gallic acid equivalent (GAE)/g extract residue (ER), respectively. Total flavonoid contents were 2.94, 9.92, 7.77, 9.27, 5.11, and 2.66 ㎎ catechin equivalent (CE)/g ER, respectively. Total tannin contents were 8.75, 10.04, 7.42, 17.32, 11.65, and 7.61 ㎎ tannic acid equivalent (TAE)/g ER, respectively. Total proanthocyanidin contents were 346.09, 63.50, 103.76, 288.62, 231.99, and 358.48 ㎍ CE/g ER, respectively. DPPH radical scavenging activities of solvent fractions from methanolic extract of sericea lespedeza were 20.62, 5.16, 9.29, 20.80, 20.00 and 20.79 ㎎ Trolox equivalent (TE)/g ER, and ABTS radical scavenging activities were 33.86, 9.24, 17.36, 33.76, 33.49, and 33.86 ㎎ TE/g ER, respectively. SOD-like activities were 4.12, 0.61, 2.01, 9.89, 13.47, and 11.82 units/mL, and α-glucosidase inhibition activities were 93.85 and 61.64% at concentrations of 50 and 25 ㎍/mL in the water fraction, respectively. The results of this study show that notable antioxidant activities in sericea lespedeza have significant health benefits.

난백분말, 포도당 및 유제품으로 만든 요구르트에서 젖산균의 생육과 산생성의 경시적인 변화

고영태 德成女子大學校 1997 德成女大論文集 Vol.28 No.-

Changes in titrable acidity, pH, viable cells and residual glucose during 30hr fermentation by L. acidophilus in a mixture of egg white powder(3%, W/V), glucose(2%, W/V) and four kinds of milk products(4%, W/V) were studied. Acidity of milk sample(control) had been lover than that of samples containing milk product until 12hr fermentation, while it had been higher than that of samples containing milk product after 18hr fermentation. Acidity of sample containing casein was higher than that of other samples containing milk product. pH of milk sample had been higher than that of other samples until 18hr, while it was not significantly different from that of other samples after 18hr. pH of sample containing whey powder was slightly lower than that of other samples containing milk product. Number of viable cells of milk sample had been slightly higher than that of other samples containing milk product after 24hr fermentation. It is likely that log phase of L. acidophilus in these samples was approximately 12hr and stationary phase of this organism continued until 30hr. concentration of residual glucose in these samples decreased almost directly proportional to fermentation time.

대퇴 전자간 골절에서 금속정 고정술 시행결과 : 감미정과 근위 대퇴정의 비교 Comparision Gamma Nail and proximal Femoral Nail

고상봉,조명래,김태훈,장일웅 대한골절학회 2004 대한골절학회지 Vol.17 No.4

목적: 대퇴 전자간 골절 환자에서 감마정과 근위 대퇴정으로 내고정을 시행하고 방사선학적, 임상적 결과 및 합병증을 비교하여 각각의 장단점을 분석하고자 하였다. 대상 및 방법: 1999년 1월부터 2002년 2월까지 대퇴 전자간 골절로 감마정과 근위 대퇴정을 이용하여 고정한 환자 중 방사선학적 및 임상적으로 6개월 이상 추시가 가능하였던 63예를 대상으로 하였으며 감마정군이 31예, 근위 대퇴정군이 32예이었다. 연령, 골절의 형태와 안정성에 따른 분류, 동반 질환, 골다공증의 정도, 환자의 활동 능력 등이 양 군에서 차이가 없었다. 평가방법으로는 두 군 사이의 수술시간, 출혈량, 안정정복 획득의 감입 정도 등을 비교하였다. 결과: 수술시간은 감마정군은 평균 103±40분, 근위 대퇴정군에서 101±28분으로 통계적인의미는 없었으며 (p=0.801), 수술 중 출혈량은 근위 대퇴정군에서 적은 경향을 보였으나 통계적인 의미는 없었다(p=0.601). 술 후 골절의 안정성 획득 여부는 감마정군 30%, 근위 대퇴정군 31%에서 안정 정복을 얻어 사용기구에 따른 고정의 안정성 획득에는 유의한 차이가 없었으며(p=0.934), 술 후 내반 고정의 빈도와 골절부위의 1.28단계의 보행능력 감소를, 근위 대퇴정군에서 평균 0.86단계의 보행능력 감소를 보여 역시 통계적으로 의미있는 차이는 없었다(p=0.383). 결론: 대퇴 전자간 골절치료에 있어서 임상적 및 방사선학적인 결과에서는 의미있는 차이는 없었으나, 근위 대퇴 골수정이 수술시간, 출혈량 등의 수술 수기의 측면에서 조금 더 우수한 경향을 보였다. Purpose: To evaluate the clinical and radiologic results of the fracture using the Proximal Femoral Nail(PFN) or Gamma nail in patient with the intertrochanteric fracture. Materials and Methods: From January 1999 to February 2002, we reviewed 63 patients of intertrochanteric fractures including 31 patients managed with Gamma nail and 32 patients with PFN. In variate analysis of age, the pattern and stability of fracture, bone density(Singh index), preoperative patient activity, there was no difference between two groups. We evaluated the operation time, blood loss, stable reduction rate and varus reduction rate as operation-related factors, and radiologically investigated union, time, the frequency of delayed union and nonunion, failure of fixation, status of the fracture line and complications. Functional results were evaluated by the possibility of early ambulation, ambulation ability at last follow up, and the pre and postoperative difference of ambulatory ability. Results: Mean operation time was 103±40 minutes in Gamma nail group 101±28 minutes in PFN group. It was not statistically significant(p=0.801). Even though intraoperative blood loss was less in PFN group, it was significant(p=0.601). Postoperative stable reduction rate was 31% in Gamma nail group and 30% in PFN group(p=0.934), suggesting not significant. Postoperative varus reduction and impacted amount of the fracture ling was not significant. Delayed union developed in one case of Gamma nail group and 2 cases of PFN group. Mean loss of ambulation ability was 1.28 grade in Gamma nail group and 0.86 grade in PFN(p=0.383). Statistical analysis was performed with the use of Student T test and Chi-square test. Analyses resulting in a p value of <0.05 were considered to show significance. Conclusion: Generally, the radiologic and clinical results of intertrochanteric fractures were comparable for the PFN group and Gamma nail group. Even though it was not statistically significant, PFN group had superior results in operation time blood than Gamma nail group for the fixation of the patients with interchanteric fracture.

여대생의 체형과 Diet 식품에 관한 의식구조 조사

고영태,강정화,김태은 德成女子大學校 1999 德成女大論文集 Vol.30 No.-

A survey on the state of dietary habit, degree of obesity, viewpoint about body shape, BMI calculated from subjcts' height and weight, amount of body fat measured by body fat computer and viewponit about diet food and drug for 196 female university students was undertaken. The results are as follows : (1) In response to the question about activity frequency, 38.8% and 27.6% of the subjects answered as "less than once per month" and "1 or 2 times per week" , respectively. In case of activity intensity, 59.2% of the subjects answered as "moderate aerobic activity". Activity time of 32.1% and 29.1% of the subjects was "under 10 minutes" and "10-20 minutes", respectively. Activity level of 54.1% and 43.9% of the subjects was "light" and "moderate", respectively. (2) Distribution of measured body fat showed that 51.0% of the subjects were in the range of "30-35%" and 27.0% of the subjects were in the range of "25-30%". The average value of 196 subjects was 31.03%. In response to the question about body shape, 43.4%, 41.3%, 9.7% and 5.6% of the subjects answered as "plump", "normal", "obese" and "thin", respectively. However, BMI calulated from subjects' height and weight showed that 1.0% of the subjects were "obesity", 0.5% were "overweight", 41.8% were "normal" , and 56.6% were "underweight". (3) The results of fitness obtained from body fat & fitness computer showed that fitness of 68.4% of the subjects were "very poor" and that of 25.0% were "poor". The results of longevity showed that longivity of 69.4% of the subjects were "good" and that of 24.0% were "excellent". (4) 11.7% of the subjects had experience of diet food or drug intake. 43.5% of the taken subjects had effect only during taking period, 34.8% had no effect, and 13.0% had some side effect.

수도권 지역 5개 여자고등학교 학생들의 체중 조절 실태와 Diet에 대한 의식구조 조사

고영태,강정화 德成女子大學校 1998 德成女大論文集 Vol.29 No.-

A survey on state of dietary habit, degree of obesity, body weight control, medium of nutritional knowledge and viewpoint of diet food and drug for 488 female students in 5 female high schools in metropolitan area was undertaken. The results are as follows:(1) In response to the question about body shape, 44.7%, 38.9% and 8.2% of the subjects answered "plump","normal", and "thin", respectively. However the results. calculated from subjects' height and weight by BMI were that 2.9% of the subjects were "overweight", 52.3% were "normal", and 43.4% were "underweight". (2) Interest of body weight control was relatively high as 32.6% of the subjects answered "high", and 41.0% did "normal". But 55.3% of the subjects did not practice any particular activity for health. 68.9% of the subjects had experience of body weight control. The reasons of body weight control were "obese or overweight" (42.0% of the subjects), "normal weight but for beauty"(40.0% of the subjects). In order to control body weight, 30.5% of the subjects practiced "control of eating volume constantly", 29.3% of the subjects pracitced "exercise" and 24.8% of the subjects practiced "exercise and diet together". (3) Nutritional knowledge was obtained "from friends or neighboring persons" (39.1%), "from newspapers or magazines" (28.5%) and "from TV or broadcasting" (25.6%).(4) 23.4% of the subjects had experience of diet food or drug intake. 36.0% of the taken subjects had effect only during taking period, 29.8% had on effect, and 26.3% had some effect.

교육 경쟁력 제고를 위한 교원의 선결과제와 해결방안

고완태,김영아,장동원 전주교육대학교 1995 論文集 Vol.31 No.-

The main goal of this article is to show principal problems to be primarily solved in teachers' society and explore soving methods about them for educational competitive force's enhancement. Recently, Korea and pricipal advanced countries of the world which recognized that the criterion of national development exists in educational development are making efforts, to educational reforms for improving educationl quality. Such a educational quality and education's success or failure directly relate to teachers' problems, so they should be primarily solved. They are as follows. First, insufficent incentives to secure well-qualified teacher candidates. Second, teachers' low morale, reduced effort and lower job satisfaction because of poor economic compensation, low social status, noninstructional duties, and inadeqate teaching environment and so on. Third, lack of teachers' professionalism, (e. g., poor professional development opportunities, teachers' professional quality about their charged subjects and job. Fourth, intricate coflicts among teachers, (e. g., poor administrative leadership, that is, principls's authoritative leadership style and low staff collegiality) To these problems, First, increasing dramatically teacher's salary up to adequate level according to their careers and professionalism, Teanher's salary should be given to elevate their moral and commitment to education because it has finally influence upon educational effectiveness. Second, improving teacher's working environment. The school's commitment to provide a safe and attractive working environment is a key indicator of whether it is a good place to work.(e. g., an efficent heatingand cooling system, a cmfortable faculty lounge, an ample parking area, adequate -sized office or workplace) Third, preparing various and attractive incentive system for securing well-qualified teacher to be. (e. g., expanding a variety of scholarship for good candidates for teaching, a good teaching physical working conditions descrived above, and various fringe benefits and so forth) Finally, upgrading the degree of social recognition of teachers' society to recover self-respect and pride of teachers, (e. g., enacting a positive, powerful, and special law to gurantee teacher's social, economic status)

우유와 과즙으로 만든 발효유를 동결건조한 제품의 관능성 조사

고영태,오미화 德成女子大學校 1998 德成女大論文集 Vol.29 No.-

Fermented milk was prepared from milk or mixture of milk and apple juice/ grape juice and it was freeze dried. The sensory properties of freeze dried and freeze dried/ reconstitutied fermented milk sample were invesitgated. In case of freeze dried/reconstituted samples, the sensory properties of the mixed sample of fruit juice-milk were better than those of milk sample. Among several different ratios of fruit juice and milk, sample of 15:35 showed the best sensory property. When the sensory properties of freeze dried apple juice-milk sample and those of freeze dried grape juice-milk sample were compared, there was no significant difference (p>0.05). However, hedonic scale score of smaple added with apple juice was slightly higher than that of sample added with grape juice(6.55 vs. 6.28).

여대생의 음주 실태와 음주에 대한 의식 구조

고영태 德成女子大學校 1995 德成女大論文集 Vol.24 No.-

A survey on drinking state, drinking habit and viewpoint on drinking of 657 students of women's university in Seoul was undertaken. The results are as follows : 1. Among 657 subjects investigated, 93.2% and 6.8% of the subjects were drinkers and nondrinkers, respectively. 2. 47.7% and 37.1% of the subjects drank several times in a month and several times in a year, respectively. 75% and 22.4% of the subjects consumed beer and soju, respectively. The most frequent drinking amount was moderate(43.1%). 65.7% of the subjects used beer hall. The most frequent choice factor of drinking place was atmosphere(60.1%). The most frequent drinking companion was female friend(60.6%). The degree of preference for specific brand of alcoholic beverage was moderate(46.2%). 60.1% of the subjects drank on weekend. The most frequent drinking cost for each person was 5000 to 20000 won(78.4%). 3. Most of the subjects drank for sociable reason. Young women's drinking was favorably accepted by the subjects. The subjects' opinion on the effect of alcohol was generally negative. Most of the subjects thought that the level of Korean drinking culture was medium or low and excessive drinking of men was the serious problem in Korean society. It is expected that more precise and logical survey on drinking state and viewpoint on drinking of Korean women should be undertaken on the basis of this research.