Production and Characterization of a Thermo-alkalotolerant Cyclodextrin Glucanotransferase from Thermo-alkalophilic Bacillus cereus B-13

Seo, Seung-Bo,Kim, Jae-Ho,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13 세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13을 2% 가용성 전분, 1% 효모 추출물, 1% Na_(2)CO_(3) 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 50℃에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 65℃, pH 8.5-9.0 이었고 80℃이하와 pH 5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용 시켰을 때 49%의 CD 수율을 보였다. To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isolated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1% Na_(2)CO_(3) (pH 8.5) at 50℃ for 24 h and about 80% of maximal activity was also showed in the culture broth of 60℃ for 18 h . Optimum reaction temperature and pH of the partial purified CGTase for soluble starch were 65℃ and pH 8.5-9.0, respectively. The partial purified CGTase were also stable below 80℃ and pH 5.0-10.0. When 1% soluble starch was digested with the partial purified CGTase, the yield of cyclodextrin was 49%.

인조 합성 테니스 스포츠 바닥재의 운동생리학적 분석

서혜림,박승범,서국웅,이재규,서혜진 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to investigate the changes of heart rate(HR), Ventileation, Carbon elimination, Oxygen take, Oxygen uptake per weight, Respiratory Exchange ratio and Breath frequency. To do this, we selected three Synthetic tennis playing surface(A:Synpave Rebound Ace Sample, B:Synpave Rebound Classic Sample, C:Synpave Spring Sample) by using of Austrilian Tennis Open. The result of this study is as follows 1. In the changes of heart rate(HR, beats/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample C, sample C was shown a little bit higher than sample B, 2. In the changes of Ventileation(VE, 1/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C, sample C was shown a little bit higher than sample A, 3. In the changes of Carbon elimination (Vco2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 4. In the changes of Oxygen take (Vo2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 5. In the changes of Oxygen uptake per weight(Vo2/wt, ml/kg/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 6. In the changes of Respiratory Exchange ratio(RER, Vco2/Vo2), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample C was shown a little bit higher than sample B, 7. In the changes of Breath frequency (BF, fre/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C.

人間의 性格類型이 手指機能에 미치는 影響(Ⅰ)

서승록 대구대학교 산업기술연구소 1987 産業技術硏究 Vol.6 No.-

This study aims at examining the kinetic aspect of finger function of human. For characters were divided through the working-character test- by a pattern of Yuhata Kohata and in each character, five men were slected by random sample. In this case, this research observed the change of finger function in left and right each. The result was that the work of using the whole finger was now th difference between characters but the work of using the first finger and second finger was that choloric temperament effect in the biggest one of factors.

에어로빅 댄스 Kick 동작의 운동역학적 분석

서국웅,이훈식,윤양진,정미라,박승범 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

The purpose of this study is to investigate the front and side Maximum Vertical Ground Reaction Force's of Knee-up, long-kick and high-kick, the effect of them speed rate on Maximum Vertical Ground Reaction Force, and the damage factor caused by them, and to provide useful data for ordinary people or athlete aerobic programs. The result of this study is as follows ; 1. Maximum Vertical Ground Reaction Force is not significant between the front and the side. 2. Maximum Vertical Ground Reaction Force is significant between differences of speed rate. 3. Maximum Vertical Ground Reaction Force, speed and direction being equal, is significant according to the motion types: knee-up, long-kick and high-kick. 4. The possibility of causing damage is the highest when Maximum Vertical Ground Reaction Force is more than 20BW/sec. 5. High-kick, which needs quick and heavy motion, is considered not be fit for beginners. 6. Load-rate must be lowered by choosing adequate speed rate and motion type, and even in the career of skilled athletes, enough exercise and muscle strength training of legs are necessary in doing aerobic.

급성 B형 간염의 회복 후 말초혈액 단핵구에서의 HBV DNA검출

명승재,이영상,김형건,이근찬,박능화,정영화,서동진 大韓消化器病學會 1997 大韓消化器病學會誌 Vol.29 No.1

전문 런닝화 개발을 위한 런닝화 화형 분석 : 2000년 런닝화를 중심으로

서국웅,윤양진,박승범,김용재 부산대학교 체육과학연구소 2000 體育科學硏究所 論文集 Vol.16 No.-

This study was designed to investigate the problem of the existing Running Standard Last for the Male adult and to suggest some useful information for the better design development and construction of running shoes last. The subjects for this study are based on 4 male adults who had the running pattern of rearfoot strike with normal feet. 51 separate measurements of four lasts of running shoe with N, R, AD, AS brand were tested using a 3-mentional measurement. Four running shoes and a barefoot were measured using a pressure distribution measurement. The findings of the study were as follows 1) The N running shoes last was larger than those for R, AD and AS the standard in the 8 items. The AS running shoes last was larger than those of N, R, AD last in the 8 items. The R's last was the biggest in short heel girth and long heel girth. The AD's last was the biggest in seat length and firth toe height 2) In the regional peak pressure of the running sheos, the barefoot and brand running shoes showed the biggest pressure distribution in the hallux, There were the slightest pressure in the medial midfoot and lateral midfoot. 3) The instance of regional peak pressure, the barefoot and the running shoes showed earlier peak pressure for the lateral heel. It was barely possible to measure pressure distribution in medial midfoot. 4) In the light of time percentage of peak pressure distribution measurement in each mask, N running shoes showed the longest time in medial heel, lateral heel, medial midfoot, lateral metatarsal and lateal toes, AD running shoes showed the longest in first metatarsal, second metatarsal and hallux, R running, the longest in second toes. 5) In the maximum force for pressure distribution, barefeet showed the biggest pressure distribution in second metatarsal and R, AD running shoes the biggest in first metatarsal, respectively. 6) In the time of peak pressure distribution measurement, barefeet quickly showed peak pressure distribution in the lateral midfoot. Simultaneously, N. R, AD and AS's running shoes showed peak pressure distribution with barefeet. 7) In the characteristic of the new last's model, bottom length resulted in 276.00mm, stick length 283.29mm, 70% ball mirth 255.12mm, bottom width 91.21mm, toe thickness 30.00mm.

두호리 공룡 발자국화석에서 본 공룡의 행동

서승조 진주교육대학교 과학교육연구소 2000 科學敎育硏究 Vol.26 No.-

Three well preserved trackways and one other trackway are found at Dooho-ri, in Goseong County. At the site A, a quadrupedal Sauropod's trackway shows that a trackway, about 20ms long changed its direction from 170˚ to 220˚ in azimuth. It seems that the trackmaker walked along the curved lake-shore line at that time. And at the site B, three bipedal tracks are observed. Trackmaker Dino-1 and -2 walked toward 250˚ in azimuth(approximately WSW) and Dino-3 crossed their trackway from Southwest to Northeast. The study of the variation of Dino-1's pace and direction leads to the conclusion that Dino-3 may have interfered with Dino-1's itinerary.

Substance P의 기니피그 위 유문동 윤상근에 대한 수축 및 전기적 효과

서종옥,이승곤,김종승,전제열,여철호,윤평진 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

한국인의 구강위생용품 사용실태에 관한 조사연구

서은주,신승철,서현석,김은주,장연수 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

The purpose of this study was to evaluate the actual state of Koreans' behavior about the use of oral hygiene devices and to collect the baseline data for developing appropriate educational objectives to improve dental health. Questionnaires were used to personally interview 1,685 residents in Korea. The obtained results were as fallows; 1. Average head size of toothbrushes was 23.2㎜ in children, 28.2㎜ im adults. 2. The averagep percent of using dentifrice containing fluoride at home was 66.7% in children, 71.4% in adults. 3. The average percent of using orthodontic toothbrushes was 50.5% in orthodontic patients, and the average percent of using interdental burshes was only 16.9% in orthodontic patients. 4. The average percent of using dental floss was 22.3% in periodontic patients, and the average percent of using interdental brushes was 16.5% in periodonitc patients. 5. The patients usually use oral hygiene devices by means of dentals' suggestions and commercial advertisement. 6. Generally few people use auxiliary oral hygiene devices. To improve the oral health starus effectively, more improved and correct informations and materials should be provide to dentists, dental hygienists and patients.

Polyaaccharide 類의 固形分斯體에 의한 藥物 傳達體系의 開發

서성훈,김길수,김동현,이승진,장미경 경희대학교 약학대학 1994 慶熙藥大論文集 Vol.22 No.