Production and Characterization of a Thermo-alkalotolerant Cyclodextrin Glucanotransferase from Thermo-alkalophilic Bacillus cereus B-13

Seo, Seung-Bo,Kim, Jae-Ho,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13 세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13을 2% 가용성 전분, 1% 효모 추출물, 1% Na_(2)CO_(3) 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 50℃에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 65℃, pH 8.5-9.0 이었고 80℃이하와 pH 5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용 시켰을 때 49%의 CD 수율을 보였다. To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isolated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1% Na_(2)CO_(3) (pH 8.5) at 50℃ for 24 h and about 80% of maximal activity was also showed in the culture broth of 60℃ for 18 h . Optimum reaction temperature and pH of the partial purified CGTase for soluble starch were 65℃ and pH 8.5-9.0, respectively. The partial purified CGTase were also stable below 80℃ and pH 5.0-10.0. When 1% soluble starch was digested with the partial purified CGTase, the yield of cyclodextrin was 49%.

초등학교 과학과 에너지영역의 STS 내용 변천

서승조,조태호,백남권,김성규,박강은,정인재 晋州敎育大學校 科學敎育硏究所 2001 科學敎育硏究 Vol.27 No.-

This research compared and analyzed the content ratio of the STS (Science-Technology-Society) Education and subjects and types of learning activities to determine the characteristics of STS education in science classes of elementary schools. In the 5th curriculum, the STS-related content on energy section took up 6.6% , in the 6th curriculum, compared with that of the 5th, the STS content ratio on the energy section increased 7.9% and the STS content on the energy section in the 7th curriculum showed an increased 20.8% proportion. Comparing the main subjects of the STS in the energy section for the science class according to curriculum in the 5th, they obviously have influenced technological development, energy, the development of space studies, and sociology of science, in that order. In the 6th curriculum. compared with that of the 5th, the energy section decreased and the sociology of science increased much. There were no significant changes in other subjects. In the 7th, the STS elements were distributed evenly but the extent of the STS main subjects reduced compared to that of the 6th. The STS education activities represented in science textbooks of elementary schools were mainly executed through discussion, research activities, and actual activities. Other activities such as role-playing, simulation tests, problem solving and decision making, data analysis, research design, case studies, etc. were hardly made.

인조 합성 테니스 스포츠 바닥재의 운동생리학적 분석

서혜림,박승범,서국웅,이재규,서혜진 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to investigate the changes of heart rate(HR), Ventileation, Carbon elimination, Oxygen take, Oxygen uptake per weight, Respiratory Exchange ratio and Breath frequency. To do this, we selected three Synthetic tennis playing surface(A:Synpave Rebound Ace Sample, B:Synpave Rebound Classic Sample, C:Synpave Spring Sample) by using of Austrilian Tennis Open. The result of this study is as follows 1. In the changes of heart rate(HR, beats/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample C, sample C was shown a little bit higher than sample B, 2. In the changes of Ventileation(VE, 1/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C, sample C was shown a little bit higher than sample A, 3. In the changes of Carbon elimination (Vco2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 4. In the changes of Oxygen take (Vo2, ml/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 5. In the changes of Oxygen uptake per weight(Vo2/wt, ml/kg/min), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample B was shown a little bit higher than sample C, 6. In the changes of Respiratory Exchange ratio(RER, Vco2/Vo2), it is not significantly in a stastics. Tennis playing surface sample A was shown a little bit higher than sample B·C, sample C was shown a little bit higher than sample B, 7. In the changes of Breath frequency (BF, fre/min), it is not significantly in a stastics. Tennis playing surface sample B was shown a little bit higher than sample A·C.

고려시대 사찰 주불전의 건축 특성 연구

서지은,홍승재 대한건축학회 2004 대한건축학회논문집 Vol.20 No.12

This study is the architectural characteristics of main Buddhist sanctum in Koryeo period. The study regarding to the Temples built in the age of Koryeo Dynasty of which most part in the research for Buddhist architectural history is yet remained left behind as a vacant period due to limitation of the relevant materials thereof. Recently, as being carried out the excavation on those relics of Koryeo temples, the architectural features for Koryeo temples are also being clarified. The plan style of main Buddhist sanctum founded in the Koryeo Period and the composition of the Buddhist sanctum are relatively various. The plane style was examined in regard to the religious contents and Buddhist consciousness at that time. Also, based on the plane structure, it was estimated whether they were single-storied or multiple-storied. For this, they were analyzed by comparing with the existing buildings of the Choseon Period.

조루증 환자 치료에서 Sertraline 필요시 자가복용요법: 단기 추적 결과

백재승,임대정,서성일,김수웅 大韓泌尿器科學會 1998 Korean Journal of Urology Vol.39 No.9

Substance P의 기니피그 위 유문동 윤상근에 대한 수축 및 전기적 효과

서종옥,이승곤,김종승,전제열,여철호,윤평진 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

섬이 있는 포켓의 가공을 위한 최적공구경로의 결정에 관한 연구

서만승,송재성,김혜령 동명정보대학교 2000 東明情報大學校論文集 Vol.3 No.-

In area milling such as pocket machining with islands, optimal tool path planning is very difficult problem. In this paper, new algorithm is presented, which determine it. Our algorithm is consisting of three modules; (1)generating the offset curves, (2)constructing and removing the offset loop and between loops, (3)finding the shortest path for minimal tool movement, and the uncut areas. For optimal tool path planning, we make use of the concepts of loop entity, which is chain of offset curves of contour curves. It is an entity that treat systematically both the intersection of offset curves and detection of an unuseful offset curves. And our algorithm is also applied to path planning for uncut area without the modification of our algorithm. Emprical tests show that the proposed algorithm fulfils its requirements.

웨이브렛 신경회로망을 이용한 비선형 시스템의 안정한 직접 적응 제어

서재용,연정흠,원경재,서승진,전홍태 중앙대학교 생산공학연구소 1998 생산공학연구소 논문집 Vol.7 No.2

이 논문에서는 웨이브렛 신경회로망을 사용하여 알려지지 않은 비선형 시스템을 안정하게 적응 제어하는 문제를 다룬다. 비선형 시스템의 정확한 제어는 함수를 근사화하는 데 상용된 함수 근사화기의 정확성과 효율성에 의존한다. 이에 기준 함수의 선택이 자유롭고 함수 근사화 능력이 뛰어난 웨이브렛 신경회로망을 비선형 시스템 제어에 사용한다. 초기 웨이브렛 신경회로망 제어기를 설정하는 방법은 먼저 제어기 입력의 시-주파수 특성을 분석해서 웨이브렛 신경회로망 변수인 신축과 이동을 구하고, 다음에 Lyapunov 안정성 이론에 기초한 적응 법칙을 사용하여 연결 강도를 조절하는 것이다. 이 직접 적응 웨이브렛 신경회로망 제어기를 불안정한 비선형 시스템인 역 진자 시스템을 제어하는 데 적용한다. In this paper, we deal with the problem of controlling an unknown nonlinear dynamical system, using wavelet network. Accurate control of the nonlinear systems depends critically on the accuracy and efficiency of the function approximator used to approximate the function. Thus, we use wavelet network which shows high capability of approximating the functions and includes the free-selection of the basis functions for the control of the nonlinear system. The method of constructing an initial adaptive wavelet network controller is that we find the dilation and translation that are wavelet network parameters by analyzing the time-frequency characteristics of the controller's input, and then, the weight is adjusted by the adaptive law based on the Lyapunov stability theory. We apply this direct adaptive wavelet network controller to control the inverted pendulum system which is an unstable system.

FeCoB계 아몰퍼스 자성박막의 임피던스효과에 관한 연구

서강수,정승우,임재근,신용진 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-

In this paper, we has studied frequency dependence of impedance of FeCoB amorphous magnetic films. First, we investigated optimal composition and sputtering conditions for fabrication of zero magnetostrictive amorphous magnetic films. (Fe_(1-x)Co_x)_79Si_2B_19 was selected as the basic composition and then, the films were fabricated with x=0.94 and 0.95 respectively by using sputtering method at high frequency. In order to relieve the internal pressure and derive the magneto-anisotropic. we annealed the films in about 150[Oe] of magnetic field and in the range(280℃, 300℃ and 320℃ respectively) of crystallization temperature(360℃). In addition, we annealed the films under non-magnetic field and near crystallization temperatures (283℃/30min, 400℃/30min and 400℃/1hr respectively) in order to decrease magnetoanisotropic. Then, we investigated magnetic properties including magneto-impedence and inductance. As the results, we found excellent magnetic characteristics and high impedence changing rate(4.52[%/Oe]) when we fabricated a sample under the condition that input power 400[W], Ar gas pressure 6.4[mTorr]. sputtering time 30[min] and annealing time 30[min] at 300[℃] in 150[Oe] magnetic field Especially, the impedence changing rate which was basic characteristic of Ml element was 4.52[%/Oe] for x=0.94. Moreover, the inductance values of the samples annealed at 400[℃/30min] were higher by 132[%] to 197[%] than those of the non-annealed samples. In addition, the quality factor Q was below 0.7 for all samples. Consequently, we find that the zero magnetostrictive amorphous magnetic film can be used as a magnetic sensor material.

30-60대 건강한 서울시민의 脚筋伸展파워 및 신체작업능력 : 동경 및 북경시민과의 비교 Comparison of Tokyo and Peking Residents

서재균,김승일,최영렬,방영진,김형돈,전병관,선우섭,손두옥 한국학교체육학회 1999 한국학교 체육학회지 Vol.9 No.1

본 연구는 한국인의 삶의 질 향상을 위한 국민 생활체육 진흥정책 수립에 필요한 기초자료를 제공하기 위하여, 건강한 30-60대 서울시민(313명)의 체격과 근력, 전신지구력 등의 체력을 측정하여 문화, 경제생활수준이 서로 빠른 동경시민(509명) 및 북경시민(160명)과 비교 검토하는 것을 목적으로 하였다. 각국의 연구대상에 대한 측정은 사전에 협의한 동일한 방법(동경대학 체육과학실법)과 기기를 이용하였다. 체격요인으로는 신장과 체중을, 체력요인으로는 트레드밀 보행시 (90m/분)의 심박수와 보폭 그리고 자전거 에르고미터 운동시의 PWC75%HRmax와 脚筋伸展파워를 측정하였다. 신장 및 체중, 신체질량지수는 남녀 모두 북경, 서울, 동경시민순으로 높게 나타났다. 트레이드밀 보행시의 심박수는 남녀 모두 북경, 동경, 서울시민 순으로 낮은 값을 보였으며, 신장에 대한 보폭비율은 남자의 경우에는 북경, 서울, 동경의 순으로, 여자는 북경, 동경, 서울의 순으로 높았다. 脚筋伸展파워는 60대 여자를 제외한 남녀 모두 북경, 서울, 동경시민의 순으로 높았으며, PWC75%Hrmax는 남자 40대 와 여자 30대를 제외한 모든 연령 대에서 서울시민이 동경시민 보다 높은 경향을 나타냈다. 한편 모든 피검자를 대상으로 연령과 脚筋伸展파워와의 회귀식은 남녀 각각 Y=-0.30X+28.88(n=230, r=0.61, p<.001), Y=-0.l0X +12.49(n=611, r=0.32, p<.001), PWC75%HRmax와의 회귀식은 남녀 각각 Y=-0.01+ 2.50(n=116, r=0.29, p<.01), Y=-0.l0X+1.89(n=461, r=0.20, p<.001)이였다. 따라서 30~60대 서울시민의 체격 및 脚筋伸展파워는 북경시민 보다 낮게 나타나 삶의 질 향상을 위해 보다 적극적인 사회체육 활성화 방안 마련이 요망된다. The purpose of this study was to serve as a basis for making a health promotion policy for better quality of Korean life, by examining 313 citizens of Seoul's physical and leg extension power, physical working capacity who were in their thirties to sixties, enjoying good health, and by making a comparison of tile result with that of 509 citizens of Tokyo and 160 citizens of Peking who lived in different culture and living environment. The findings of this study were as below: First, the male citizens of Peking, who were in their forties to sixties, and the female citizens of the same city, who were in their fifties to sixties, were significantly higher in height, body weight and body mass index. Second, the male citizens of Peking, who were in their thirties and sixties, and the female citizens of the same city, who were in their thirties to forties, were significantly lower in heart rate in walking in the treadmill(90n/min). And there was a tendency that the citizens of Peking showed a higher ratio of stride to height. Third, the male citizens of Peking, who were in their fifties, and the female citizens of the same city, who were in their thirties to forties, had a significantly better leg extension power. As a result of examining all the subjects, there was a negative correlation between leg extension power and age. Fourth, all age groups of Seoul citizens, except males in their forties and females in their thirties, fended to be better in PWC75%HRmax than the citizens of Tokyo. When all the subjects were examined, there was a negative correlation between PWC75%HRmax and age. Consequently, there was a need to prepare more active plan to step up sports fur all successfully in order to improve the quality of life, as the citizens of Seoul who were their thirties to sixties appeared to be inferior in physical physique and leg extension power to the citizens of Peking.