Suppression of lung inflammation by the methanol extract of <i>Spilanthes acmella</i> Murray is related to differential regulation of NF-κB and Nrf2

Kim, Kyun Ha,Kim, Eun Jung,Kwun, Min Jung,Lee, Ji Yeon,Bach, Tran The,Eum, Sang Mi,Choi, Jun Yong,Cho, Sayeon,Kim, Sang-Jun,Jeong, Seung-Il,Joo, Myungsoo Elsevier 2018 Journal of Ethnopharmacology Vol.217 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Although <I>Spilanthes acmella</I> has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of <I>S. acmella</I> Murray (SAM).</P> <P><B>Materials and method</B></P> <P>The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.</P> <P><B>Results</B></P> <P>The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.</P> <P><B>Conclusion</B></P> <P>Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of <I>S. acmella</I>.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Interaction between Brain-Derived Neurotrophic Factor Val66Met Polymorphism and Recent Negative Stressor in Harm Avoidance

Kim, Seog Ju,Cho, Seong-Jin,Jang, Ha Min,Shin, Jonghan,Park, Pil-Whan,Lee, Yu Jin,Cho, In Hee,Choi, Jung-Eun,Lee, Heon-Jeong S. Karger AG 2009 Neuropsychobiology Vol.61 No.1

<P>There have been controversial results regarding the association between brain-derived neurotrophic factor (BDNF) Val66Met polymorphism and anxiety-related traits such as harm avoidance (HA). We aimed to investigate the interaction between <I>BDNF</I> Val66Met polymorphism and negative life stressors in HA. <I>BDNF</I> Val66Met polymorphism was genotyped in 391 community-dwelling Koreans (152 males, 239 females; 43.2 ± 14.1 years old). The Temperament and Character Inventory (TCI) and the Center for Epidemiological Studies for Depression Scale (CES-D) were self applied. The Structured Clinical Interview for DSM-IV axis I disorders and face-to-face interviews investigating negative life stressors within the last 6 months were also performed. There was no significant difference in TCI score, major depressive disorder prevalence and CES-D score among the 3 genotypes (94 Met/Met, 188 Val/Met and 109 Val/Val subjects). There was no significant difference in TCI scores between subjects with stressors and those without stressors, while more common major depressive episodes (p = 0.03) and higher CES-D scores (p < 0.001) were found in subjects with stressors. However, there was a significant interaction between the <I>BDNF</I> genotype and negative life stressors in HA (p = 0.02). Only subjects with the Val/Val genotype showed higher HA with recent negative stressors. Our finding suggests that <I>BDNF</I> Val66Met polymorphism might influence HA by interacting with recent negative stress experience.</P><P>Copyright © 2009 S. Karger AG, Basel</P>

Systematic Review of Heavy Metal Concentrations in Fish and Shellfish in Korea

Shinhee Ye,Jiyoung Shin,Jueun Lee,Eun Mi Jung,Jeongsook Lee,Eunsun Yun,Yeosook Kim,Younghee Oh,Eun-Hee Ha 이화여자대학교 의과학연구소 2018 EMJ (Ewha medical journal) Vol.41 No.1

Objectives: Heavy metals ingested through the consumption of aquatic products can accumulate in the human body over the long-term and cause various health problems. This study aims to present comprehensive data on the amount of heavy metals found in fish and shellfish in Korea using a systematic review of studies that report on that issue. Methods: The study used the following databases: PubMed, Korean Studies Information Service System, and Research Information Sharing Service. The search terms for PubMed included fish OR shellfish OR seafood AND mercury OR cadmium OR lead OR heavy metal AND Korea. The search terms for Korean Studies Information Service System and Research Information Sharing Service included eoryu sueun, eoryu kadeumyum, eoryu nab, eoryu jung-geumsog, paeryu sueun, paeryu kadeumyum, paeryu nab, paeryu jung-geumsog, eopaeryu sueun, eopaeryu kadeumyum, eopaeryu nab, and eopaeryu jung-geumsog. Results: A total of 32 articles were selected for review. The total mercury, lead, and cadmium concentrations in fish and shellfish reported in each of the articles are summarized, as are the species of fish and shellfish with relatively high concentrations of heavy metals. Total mercury concentrations tended to be higher in predatory fish species, such as sharks, billfishes, and tuna, while lead and cadmium concentrations tended to be higher in shellfish. Conclusion: This paper is the first to report a comprehensive summary of the concentrations of heavy metals in fish and shellfish. This data could be used as evidence to protect Koreans from exposure to heavy metals due to the consumption of highly polluted aquatic products.

1996학년도 신입생 실태조사

하은정,양병한 창원대학교 학생생활연구소 1997 학생생활연구 Vol.10 No.-

동국대학경주병원에서 분리된 각종 병원성 세균 및 항생제 감수성 양상

하경임,고은하,전창호,정병욱,안우섭,김우택,배정수,어경윤 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

We investigated characteristics of bacterial infection and antimicrobial suscerptibility patterns of clinical specimens in Dong Guk University Kyong Ju Hospital from october 1991 to december 1992. The types of clinical specimens requested for culture were as follows ; pus(34.2%), urine(29.6%), sputum(25.2%), blood(9.2%), etc. The most frequently isolated organisms were E. coli(17.6%), Pseudomonas(12.7%), S. aureus(12.2%), and CNS(11.2%), orderly. Predominant organisms from pus, urine and sputum were S.aureus, E. coli and Pseudomonas. Blood culture was requested 1,244 samples from 614 patients, 91 pathogeni organisms were isolated(14.8% from patient and 7.3% from requested clinical samples). E. coli was most frequently isolated(29.7%) and Salmonella typhi was noted 8.8% from the requested samples. The patterns of isolated organisms according to departments were as follows ; E.coli and Klebsiella were frequently isolated in internal medicine and general surgery, E.coli and Pseudomas in Neurosurgery, S. aureus and Pseudomonas in orthopedic surgery. In ICU, Pseudomonas was predominatly isolated and the rate of mixed infection was also high(16.4%). Most of gram-negative bacilli revealed relatively high susceptibility to Chlorampenicol, Amikacin, Tobramycin and Carbenicillin, but Acinetobacter were only susceptible to Carbenicillin and Tetrecyclin, and Serratia were to Carbenicillin and Amikacin while Salmonella were susceptible to all tested first drugs. The proportions of multi-drug resistant bacteria were 34.9% of Acinetobacter, 21.1% of Serratia, 20.0% of Pseudomonas and 15.4% of Citrobacter but Aztreonam was highly susceptible(84∼100%) except Acinetobacter. S. aureus and CNS were susceptible to Chlorampenicol, Oxacillin, Cephalothin and Vancomycin, especially, 100% susceptible to Vancomycin. But Methicillin reistant Staphylococcus aureus were very high(49%) inspite of new constructed hospital. Pneumococcus and Streptococcus species were relatively high susceptible to all tested drugs.

학업우수 초등학생 자녀를 둔 어머니의 진로지도 경험에 관한 질적 연구

하정,이항심,정혜윤,김은덕,유성경 한국직업능력개발원 2007 직업능력개발연구 Vol.10 No.3

본 연구는 학업우수 초등학생 자녀를 둔 어머니의 진로지도 경험을 한국의 사회·문화적 맥락에서 밝히고자 하였다. 이를 위해 서울지역에 살고 있는 초등학교 6학년인 학업우수아의 어머니 9명을 심층면담하고, 질적 연구 방법으로 분석하였다. 연구 결과, 107개의 개념과 44개의 하위 범주, 그리고 14개의 범주가 도출되었다. 어머니들의 진로지도 경험을 포괄하는 핵심범주로는 '자녀의 성취를 위해 자녀의 특성을 존중하고 자녀의 자율성을 키우면서 자신이 바람직하다고 여기는 방향으로 이끌어감'으로 밝혀졌다. 연구 결과를 바탕으로 학업우수 초등학생 자녀를 둔 어머니의 진로지도 경험의 실체를 논의하였으며, 부모를 대상으로 한 진로교육프로그램의 필요성과 후속 연구에 대한 제언을 하였다. This study examined the experience about career guidance of mother who has a academically talented child in Korea. Nine mothers were interviewed and the interview data were analyzed based on the Qualitative Research Method. Result has107 concepts, 44 subcategories and 14 categories. The core category is "The mothers consider their children's characteristics for the children's accomplishment and foster children's self-control and lead their children to desirable object the mothers view".Implications of the results and suggestions for future research are discussed.

人蔘이 卵巢摘出로 誘發된 흰쥐의 骨多孔症 예방에 미치는 효능

정국영,서부일,이은숙,변부형,신순식,박지하 대한본초학회 2004 大韓本草學會誌 Vol.19 No.1

Objectives : The present study has been undertaken to investigate the effects of Ginseng Radix on prevention of osteoporosis in ovariectomized rats. Methods : In this experiment, the rats were ovariectomized. Rats were administered by 2 kinds of medicine, Estradiol, Ginseng Radix. The levels of bone mineral density, osteocalcin, ALP, phosphorus, calcium in serum, deoxypyridinoline in urine, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results : The levels of spinal bone mineral density was significantly increased in comparison with OVX group at 8 weeks in Ginseng Radix group. The levels of serum didn t show significant change in comparison with OVX group in Ginseng Radix group. The levels of urine deoxypyridinoline showed significant decrease in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial ash weight didn t show significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial calcium showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of femoral and fibula-tibial phosphorus showed significant increase in comparison with OVX group in Ginseng Radix group. The levels of body weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. The levels of uterus weight didn t show significant changes in comparison with OVX group in Ginseng Radix group. Conclusions : Reviewing these experimetal results, it appears that Ginseng Radix have efficacy on prevention of osteoporosis. And further study should be conducted to illustrate in depth the curing and prevention of osteoprosis.

뇌성마비아를 위한 운동 프로그램에 관한 연구

함정은,이동건,하형주 東亞大學校附設스포츠科學硏究所 1998 스포츠科學硏究論文集 Vol.16 No.-

The purpose of this study is to comprehend the cerebral palsied children's walking ability and to make it possible for them to start walking earlier by presenting a special exercise program that can develop walking ability. To attain this goal, I chose 7 cerebral palsied children and had them work out according to the special exercise program 5 times a week, for 90 minutes each, for 13 months. The results were as follows : 1. 5 our of 7, [JU], [SU], [KI], [HY] and [PY] could walk almost normally ; this proves the efficiency of this program. 2. The reason [KA] and [BA] couldn't improve their walking ability as much as the others is individual difference, I might sat. 3. Individual differences in each exercise program were great but resistance, tension and an involuntary motion diminished on a large scale. 4. The presented special exercise program has caused cerebral palsied childen's reflective power and basic motor control power to increase remarkably.

주의력 결핍 과잉행동장애 아동의 운동 수행이 사회기능변화에 미치는 영향

김은정,하형주 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The ADHD (Attention Deficit Hyperactivity Disorder) children (full age : 7~9) attending elementary school had golf putting exercise : 3 times a week, 10 putting each time. Measurement was made for 9 months on the standard artificial grass mat at the distances of 2.5m, 4m, and 5.5m. This experiment was accompanied with the questionnaire concerning ACTers (Attention Deficit with or without Hyperactivity Comprehensive Teacher's Rating Scale) to know the effects of the exercise on behavior adjustment in the periods of 3, 6, and 9 months. The following sums up the results from this study : 1. Attention concentration was improved to get better learning attitude and classwork. 2. The exercise learning had an insufficient effects on the decrease hyperactivity. 3. The skills of sociality were developed through the physical exercise activities. Therefore, the subjects could form sympathy with peer students and affirmative social acceptance. 4. The exercise learning brought about a little decrease in offensive tendency and animosity. 5. The exercise learning hardly affected their behavior toward the teacher and peer students. In sum, the golf putting exercise in this research had a positive effect on the behavioral change of the ADHD kids. Henceforth, it is thought to be an effective proposal for preventing, healing, and guiding ADHD, one of the important problem behavior disorders to be solved on the spot of child education.

연쇄알구균 감염에 의한 급성 인후염 후 반응성 관절염 1예

박은하,도연실,양정채,강미라,서현주,정숙인,손준성,기현균,오원섭,백경란,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

연쇄알구균에 의한 급성 인후염 후 발생한 반응성 관절염은 급성 류마티스열의 개정된 진단기준을 충족하지 못하면서, 지속적 혹은 재발성의 비이동성 관절염과 발열, 홍반성 결절, 일시적 간기능 이상 등을 보이면서 심장염과 무도증을 드물게 동반하는 질환이다. 따라서, 발열을 동반한 급성 관절염 환자에서 감별해야 할 질환 중의 하나이며, 심근염 예방을 위한 페니실린 요법의 적응증 및 기간에 대해서는 아직 이론이 많은 상황으로, 보다 광범위한 연구가 필요하다. 저자들은 연쇄알구균에 의한 급성 인후염 1주 후에 발생한 하지의 다발성 비이동성 관절염과 함께 결절홍반 및 일시적 간기능 이상을 보인 환자에서 PSRA로 진단하고 비스테로이드성 항염증제 및 스테로이드로 성공적인 치료를 하였기에 이를 문헌 고찰과 함께 보고하는 바이다. Poststreptococcal reactive arthritis (PSRA) is associated with recent streptococcal infections. However, PSRA is distinct from acute rheumatic fever by its clinical manifestations: non-migrating arthritis, erythema nodosum or erythema multiforme, and transient elevation of serum transaminases. We experienced a 33-year-old man who presented with fever, arthritis of both knees and ankles, and erythema nodosums on extensor surfaces of lower legs which developed 6 days after the onset of pharyngitis symptoms. Blood and urine cultures were negative. Throat culture was negative for group A β-hemolytic streptococcus. The ASO titers increased up to 2080 IU/mL in sequential monitoring. The result of bone scan was compatible to arthritis of both knees and ankles. There were no signs or symptom of carditis. He showed clinical improvement with anti-inflammatory drugs (naproxen 1,000 mg/day and prednisolone 7.5 mg/d). PSRA should be included in the differential diagnosis of patients presenting with arthritis combined with fever.