Effect of Whitlockite as a new bone substitute for bone formation in spinal fusion and ectopic ossification animal model

Yuan Zhe Jin,Guang-Bin Zheng,조민준,이재협 한국생체재료학회 2021 생체재료학회지 Vol.25 No.4

Background: Bone substrates like hydroxyapatite and tricalcium phosphate have been widely used for promoting spinal fusion and reducing the complications caused by autograft. Whitlockite has been reported to promote better bone formation in rat calvaria models compare with them, but no study investigated its effect on spinal fusion yet. Also, the higher osteoinductivity of whitlockite raised concern of ectopic ossification, which was a complication of spinal fusion surgery that should be avoided. Methods: In this study, we compared the osteoinductivity of whitlockite, hydroxyapatite, and tricalcium phosphate porous particles with SD rat spine posterolateral fusion model and investigated whether whitlockite could induce ectopic ossification with SD rat abdominal pouch model. Results: The micro-CT result from the posterolateral fusion model showed whitlockite had slightly but significantly higher percent bone volume than tricalcium phosphate, though none of the materials formed successful fusion with surrounding bone tissue. The histology results showed the bone formed on the cortical surface of the transverse process but did not form a bridge between the processes. The result from the abdominal pouch model showed whitlockite did not induce ectopic bone formation. Conclusion: Whitlockite had a potential of being a better bone substrate hydroxyapatite and tricalcium phosphate in spinal fusion with low risk of inducing ectopic ossification.

Comparison of demineralized bone matrix and hydroxyapatite as carriers of Escherichia coli recombinant human BMP-2

Yuan Zhe Jin,Guang-Bin Zheng,이재협,Shi-Huan Han 한국생체재료학회 2021 생체재료학회지 Vol.25 No.3

Background: Autograft has been widely used in various orthopedic and dental surgery for its superior osteogenicity, osteoinductivity and osteoconductivity. But the available volume of the autograft is limited and the efficacy of it is highly affected by the condition of the patients. Therefore, growth factors such as Escherichia coli bone morphogenetic protein-2 (ErhBMP-2) has been widely used in some countries and regions with various carriers that could affect the effects of the growth factors. Demineralized bone matrix (DBM) has been widely used as a bone graft substitute and growth factor carrier, but its effect as a carrier of ErhBMP-2 was less investigated. Materials and methods: Rat calvaria defect model was used in this study. We implanted ErhBMP-2 with DBM or hydroxyapatite (HA) as a carrier in 8mm calvaria defect and compared their bone regeneration effect in 4th week and 8th week after implantation with micro-CT and histology. The data was analyzed with one-way ANOVA method with Bonferroni post-hoc analysis. Result: The group with DBM as the carrier showed significantly higher bone volume and bone thickness than the groups with HA as the carrier in both weeks. And the histology sections showed less adipose tissue formed in the groups with DBM as the carrier. Conclusion: DBM could be a better carrier for ErhBMP-2 than HA.

Mesenchymal Stem Cell Therapy for Bone Regeneration

Yuan Zhe Jin,이재협 대한정형외과학회 2018 Clinics in Orthopedic Surgery Vol.10 No.3

Mesenchymal stem cells (MSCs) have been used in clinic for approximately 20 years. During this period, various new populations of MSCs have been found or manipulated. However, their characters and relative strength for bone regeneration have not been well known. For a comprehensive understanding of MSCs, we reviewed the literature on the multipotent cells ranging from the definition to the current research progress for bone regeneration. Based on our literature review, bone marrow MSCs have been most widely studied and utilized in clinical settings. Among other populations of MSCs, adipose-derived MSCs and perivascular MSCs might be potential candidates for bone regeneration, whose efficacy and safety still require further investigation.

Effect of Brace to Osteoporotic Vertebral Fracture: A Meta-Analysis

Yuan Zhe Jin,이재협 대한의학회 2016 Journal of Korean medical science Vol.31 No.10

Brace is one of the most commonly used interventions to manage osteoporotic vertebral fracture. However, its authentic effectiveness remains unclear. The aim of this study was to investigate the efficacy of brace in patients with osteoporotic vertebral fractures. We conducted a literature review and meta-analysis following the guideline and handbook of the Cochrane collaboration. Ten published articles were included in this study and data from 4 randomized controlled trials were analyzed. Low quality evidence proved using Spinomed brace could bring large and significant beneficial effect to patients with sub-acute osteoporotic vertebral fractures. Very low quality evidence proved no significant difference between Spinomed orthosis, rigid brace and soft brace when they were used in patients with acute fractures. Therefore, it might be applicable to recommend middle term use of Spinomed orthosis to patients with subacute fracture. In addition, this study emphasized the need for high quality randomized controlled trials.

2,5-Hexanedione induces apoptosis via a mitochondriamediated pathway in PC12 cells

Yuan Qi,Shuang-yue Li,Feng-yuan Piao,Zhe-min Wang,Ruo-lin Chen,Shuang Liu,Jing-shun Shen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.1

2,5-Hexanedione (HD) is the main active metabolite of n-hexane and mediates the neurotoxicity of the parent compound. Studies suggested that apoptosis involved in HD neurotoxicity. However, the mechanism of HD-induced neuronal apoptosis remains unknown. To explore its underlying mechanism, we treated PC12 cells with 5, 10 and 20 mM HD for 24 h, respectively. We found that HD induced apoptotic death in PC12 cells in a dose-dependent manner. Moreover, HD down-regulated Bcl-2 expression, up-regulated Bax expression and Bax/Bcl-2 ratio, promoted the disruption of mitochondrial transmembrane potential, induced the release of cytochrome c from mitochondria, and increased the activity of caspase-3 in PC12 cells, which were all the key regulators of intrinsic apoptotic pathway. These results indicate that HD induces apoptosis via a mitochondria- mediated pathway in PC12 cells.

파자마 룩의 특성 및 2015 S/S~2017 F/W 디자인 특징

신철원 ( Shen Zhe Yuan ),김차현 ( Kim Cha Hyun ) 한국기초조형학회 2017 기초조형학연구 Vol.18 No.3

최근 파자마 룩 스타일이 유행함에 따라 본 연구는 파자마 룩의 특성 및 2015 S/S~2017 F/W 디자인 특징에 대해 알아보는 것을 목적으로 파자마 룩에 대한 문헌 조사와 최근 주요 패션 컬렉션 및 사이트 등에 나타난 파자마 룩에 대한 사례분석을 하였으며 그 결과는 다음과 같다. 파자마 룩은 페미니즘, 자연주의, 미니멀리즘, 인본주의적인 특성을 가진다. 2015 S/S~2017 F/W 파자마 룩 디자인 특징에 있어서 소재는 실크가 편안하면서도 부드러운 감촉과 우아한 광택으로 유행의 선두에 있었다. 색상은 세분화된 소비자들의 수요에 맞게 다양한 색상이 폭넓게 사용되고 있었으며 특히, 전통적인 파자마 디자인에 많이 쓰이지 않았던 짙고 화려한 색상이 주를 이루었다. 무늬는 솔리드 패브릭과 큰 사이즈의 과감한 디자인이 병행하고 있었다. 형태 중 실루엣은 전형적인 H 실루엣의 여유 있는 핏이 주를 이루었고 칼라, 네크라인 깊이, 소매, 옷의 길이, 바지 통, 포켓 등의 디테일과 장식의 다양한 변화로 트렌드함을 강조하였다. 파자마 룩의 유행은 소비자의 다양한 선호 취향을 반영하는 것으로 긴장되고 바쁜 생활을 하는 현대인들이 선호하게 된 패션 라이프스타일에 연유한 것이다. 파자마 룩은 단순히 패션 디자인 측면에서의 변화가 아닌 의복 문화와 생활 방식에 대한 사람들의 사고를 심화시킨 인문 정신에 바탕을 둔 스타일이며, 그들의 관심과 선호에 초점을 맞출 수 있도록 하는 패션의 한 변화라 할 수 있다. With the current emergence of `pajama look`, this study attempted to investigate the features of pajama trend and characteristics of design from 2015 S/S to 2017 F/W. For this, literature review and case study were performed on the pajama styles in major fashion collections and websites. Then, the results found the followings: A pajama look contains feminine, naturalistic, minimalist and humanistic characteristics. In pajama styles from 2015 S/S to 2017 F/W, silk was on trend with comfort, smoothness and elegant gloss. In terms of color, a wide collection of colors were used to meet consumers` diverse needs and demand. In particular, dark and fancy colors which have been unusual in traditional pajama design were primarily used. In patterns, solid fabric and large and bold patterns were adopted. In terms of shape, a baggy style in typical H silhouette was the mainstream. With diverse changes in color, depth of neck plunge, sleeves, length of clothes, width of pants and pocket, a trendy style was emphasized. A pajama trend originates from modern people`s tense and hectic lifestyle, reflecting their diverse preferences. After all, a pajama look is a humanistic style which deepened people`s thoughts on clothing culture and lifestyles, not just change in fashion design. In other words, it is a part of change in fashion, which responds to their interests and preferences.

Effects of Applying Both Dry- and Wet-Strength Agents to Kraft Paperboard on the Resulting Physical Properties

Yuan-Shing Perng,Zhe Wei Tung,Fu Shan Zhang 한국펄프·종이공학회 2016 한국펄프·종이공학회 학술발표논문집 Vol.2016 No.10

Functional Evaluation of Transplanted Kidneys with Reduced Field-of-View Diffusion-Weighted Imaging at 3T

Yuan Xie,Yanjun Li,Jiqiu Wen,Xue Li,Zhe Zhang,Jianrui Li,Yan’e Zhao,Peng Wang,Jun Zhang,Ying Tian,Long Jiang Zhang,Guang Ming Lu 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.2

Objective: To determine the feasibility of reduced field-of-view diffusion-weighted imaging (rFOV DWI) with multi-b values to detect functional variability in transplanted kidneys. Materials and Methods: Using a 3T MRI scanner, multi-b rFOV DWI of transplanted kidney or native kidney was performed in 40 renal transplantation recipients and 18 healthy volunteers. The patients were stratified, according to an estimated glomerular filtration rate (eGFR): Group 1, eGFR ≥ 60 mL/min/1.73 m2; Group 2, eGFR ≥ 30 mL/min/1.73 m2 and < 60 mL/min/1.73 m2; Group 3, eGFR < 30 mL/min/1.73 m2. Total apparent diffusion coefficient (ADCT), perfusion-free ADC (ADCD) and perfusion fraction (FP) of kidneys were calculated and compared among the four groups. Correlations between the imaging results and eGFR were assessed. Results: All volunteers had eGFR ≥ 60 mL/min/1.73 m2, while 16, 16, and 8 patients were included in Groups 1, 2, and 3, respectively. In the renal cortex, ADCT was higher in Group 1 ([1.65 ± 0.13] x 10-3 mm2/s) than Group 3 ([1.44 ± 0.11] x 10-3 mm2/s) (p < 0.05), and the inter-group differences of FP values were significant (all p < 0.05) (0.330 ± 0.024, 0.309 ± 0.019, 0.278 ± 0.033, and 0.250 ± 0.028 for control group, Groups 1, 2, and 3, respectively). Renal cortical ADCT, ADCD, FP, and renal medullary ADCT and FP correlated positively with eGFR (r = 0.596, 0.403, 0.711, 0.341, and 0.323, respectively; all p < 0.05). When using 0.278 as the cutoff value, renal cortical FP had a sensitivity of 97.1% and a specificity of 66.7% for predicting decreased renal function. Conclusion: Multi-b rFOV DWI presents transplanted kidneys with high resolution, which is a promising functional tool for non-invasively monitoring function of transplanted kidneys.

Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

( Yuan Qing Hu ),( Jin Lin Huang ),( Qiu Chun Li ),( Yu Wei Shang ),( Fang Zhe Ren ),( Yang Jiao ),( Zhi Cheng Liu ),( Zhi Ming Pan ),( Xin An Jiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.

Study on Polysaccharide Bonded Abrasive Tool Using Hydrothermal Gelatinisation for Green Machining of Single Crystal Sapphire

Zhe Wu,Yuying Zhang,Yong Liu,Fangli Mo,Chengwu Wang,Huizong Lu,Julong Yuan,Zhifeng Liu 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.1

Aiming to green machining of single crystal sapphire, polysaccharide-bonded soft abrasive tool using hydrothermal gelatinisation method was proposed. Mechanical properties of prepared polysaccharide films show that when the weight ratio of natural polysaccharide to cross-linked polysaccharide is 1:1, the tensile strength and extension rate of the film are relatively optimal. Composition and making process of polysaccharide bonded abrasive tool was built. Samples of polysaccharide bonded soft abrasive tools were made by which the single crystal sapphire substrates were machined. It is found that by adopting guar gum as dispersion agent and hydrothermal gelatinisation, the abrasive sedimentation during making process of abrasive tool is substantially eliminated, and better abrasive holding ability is attained. XPS and Raman microscope analysis showed that material of single crystal sapphire was removed by mechano-chemical reaction with good surface integrity. Abrasive tools with abrasive to binder ratio of 4:1 presented relatively better self-sharpening and machining performance, and the material removal rate reached 0.427 μm/min. More interesting, XPS and FT-IR analyses both indicated that the polysaccharide binder dehydrated by the friction heat during machining process.