The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny

Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7

In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Emodin-Provoked Oxidative Stress Induces Apoptosis in Human Colon Cancer HCT116 Cells through a p53-Mitochondrial Apoptotic Pathway

Xie, Mei-Juan,Ma, Yi-Hua,Miao, Lin,Wang, Yan,Wang, Hai-Zhen,Xing, Ying-Ying,Xi, Tao,Lu, Yuan-Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Emodin, a natural anthraquinone isolated from the traditional Chinese medicine Radix rhizoma Rhei, can induce apoptosis in many kinds of cancer cells. This study demonstrated that emodin induces apoptosis in human colon cancer HCT116 cells by provoking oxidative stress, which subsequently triggers a p53-mitochondrial apoptotic pathway. Emodin induced mitochondrial transmembrane potential loss, increase in Bax and decrease in Bcl-2 expression and mitochondrial translocation and release of cytochrome c to cytosol in HCT116 cells. In response to emodin-treatment, ROS increased rapidly, and subsequently p53 was overexpressed. Pretreatment with the antioxidant NAC diminished apoptosis and p53 overexpression induced by emodin. Transfecting p53 siRNA also attenuated apoptosis induced by emodin, Bax expression and mitochondrial translocation being reduced compared to treatment with emodin alone. Taken together, these results indicate that ROS is a trigger of emodin-induced apoptosis in HCT116 cells, and p53 expression increases under oxidative stress, leading to Bax-mediated mitochondrial apoptosis.

Functional Evaluation of Transplanted Kidneys with Reduced Field-of-View Diffusion-Weighted Imaging at 3T

Yuan Xie,Yanjun Li,Jiqiu Wen,Xue Li,Zhe Zhang,Jianrui Li,Yan’e Zhao,Peng Wang,Jun Zhang,Ying Tian,Long Jiang Zhang,Guang Ming Lu 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.2

Objective: To determine the feasibility of reduced field-of-view diffusion-weighted imaging (rFOV DWI) with multi-b values to detect functional variability in transplanted kidneys. Materials and Methods: Using a 3T MRI scanner, multi-b rFOV DWI of transplanted kidney or native kidney was performed in 40 renal transplantation recipients and 18 healthy volunteers. The patients were stratified, according to an estimated glomerular filtration rate (eGFR): Group 1, eGFR ≥ 60 mL/min/1.73 m2; Group 2, eGFR ≥ 30 mL/min/1.73 m2 and < 60 mL/min/1.73 m2; Group 3, eGFR < 30 mL/min/1.73 m2. Total apparent diffusion coefficient (ADCT), perfusion-free ADC (ADCD) and perfusion fraction (FP) of kidneys were calculated and compared among the four groups. Correlations between the imaging results and eGFR were assessed. Results: All volunteers had eGFR ≥ 60 mL/min/1.73 m2, while 16, 16, and 8 patients were included in Groups 1, 2, and 3, respectively. In the renal cortex, ADCT was higher in Group 1 ([1.65 ± 0.13] x 10-3 mm2/s) than Group 3 ([1.44 ± 0.11] x 10-3 mm2/s) (p < 0.05), and the inter-group differences of FP values were significant (all p < 0.05) (0.330 ± 0.024, 0.309 ± 0.019, 0.278 ± 0.033, and 0.250 ± 0.028 for control group, Groups 1, 2, and 3, respectively). Renal cortical ADCT, ADCD, FP, and renal medullary ADCT and FP correlated positively with eGFR (r = 0.596, 0.403, 0.711, 0.341, and 0.323, respectively; all p < 0.05). When using 0.278 as the cutoff value, renal cortical FP had a sensitivity of 97.1% and a specificity of 66.7% for predicting decreased renal function. Conclusion: Multi-b rFOV DWI presents transplanted kidneys with high resolution, which is a promising functional tool for non-invasively monitoring function of transplanted kidneys.

Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation.

Xie, Yuan-Bin,Park, Jeong-Hoh,Kim, Don-Kyu,Hwang, Jung Hwan,Oh, Sangmi,Park, Seung Bum,Shong, Minho,Lee, In-Kyu,Choi, Hueng-Sik American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.42

<P>SMILE (small heterodimer partner interacting leucine zipper protein) has been identified as a corepressor of the glucocorticoid receptor, constitutive androstane receptor, and hepatocyte nuclear factor 4alpha. Here we show that SMILE also represses estrogen receptor-related receptor gamma (ERRgamma) transactivation. Knockdown of SMILE gene expression increases ERRgamma activity. SMILE directly interacts with ERRgamma in vitro and in vivo. Domain mapping analysis showed that SMILE binds to the AF2 domain of ERRgamma. SMILE represses ERRgamma transactivation partially through competition with coactivators PGC-1alpha, PGC-1beta, and GRIP1. Interestingly, the repression of SMILE on ERRgamma is released by SIRT1 inhibitors, a catalytically inactive SIRT1 mutant, and SIRT1 small interfering RNA but not by histone protein deacetylase inhibitor. In vivo glutathione S-transferase pulldown and coimmunoprecipitation assays validated that SMILE physically interacts with SIRT1. Furthermore, the ERRgamma inverse agonist GSK5182 enhances the interaction of SMILE with ERRgamma and SMILE-mediated repression. Knockdown of SMILE or SIRT1 blocks the repressive effect of GSK5182. Moreover, chromatin immunoprecipitation assays revealed that GSK5182 augments the association of SMILE and SIRT1 on the promoter of the ERRgamma target PDK4. GSK5182 and adenoviral overexpression of SMILE cooperate to repress ERRgamma-induced PDK4 gene expression, and this repression is released by overexpression of a catalytically defective SIRT1 mutant. Finally, we demonstrated that ERRgamma regulates SMILE gene expression, which in turn inhibits ERRgamma. Overall, these findings implicate SMILE as a novel corepressor of ERRgamma and recruitment of SIRT1 as a novel repressive mechanism for SMILE and ERRgamma inverse agonist.</P>

History and reality of Chinese spices

XIE Ding-Yuan 부산대학교 문화유산보존연구소 2008 한국전통복식연구소 학술심포지움 Vol.- No.-

Molecular characterization of SMILE as a novel corepressor of nuclear receptors

Xie, Yuan-Bin,Nedumaran, Balachandar,Choi, Hueng-Sik Oxford University Press 2009 Nucleic acids research Vol.37 No.12

<P>SMILE (small heterodimer partner interacting leucine zipper protein) has been identified as a coregulator in ER signaling. In this study, we have examined the effects of SMILE on other NRs (nuclear receptors). SMILE inhibits GR, CAR and HNF4α-mediated transactivation. Knockdown of SMILE gene expression increases the transactivation of the NRs. SMILE interacts with GR, CAR and HNF4α <I>in vitro</I> and <I>in vivo</I>. SMILE and these NRs colocalize in the nucleus. SMILE binds to the ligand-binding domain or AF2 domain of the NRs. Competitions between SMILE and the coactivators GRIP1 or PGC-1α have been demonstrated <I>in vitro</I> and <I>in vivo</I>. Furthermore, an intrinsic repressive activity of SMILE is observed in Gal4-fusion system, and the intrinsic repressive domain is mapped to the C-terminus of SMILE, spanning residues 203–354. Moreover, SMILE interacts with specific HDACs (histone deacetylases) and SMILE-mediated repression is released by HDAC inhibitor trichostatin A, in a NR-specific manner. Finally, ChIP (chromatin immunoprecipitation) assays reveal that SMILE associates with the NRs on the target gene promoters. Adenoviral overexpression of SMILE represses GR-, CAR- and HNF4α-mediated target gene expression. Overall, these results suggest that SMILE functions as a novel corepressor of NRs via competition with coactivators and the recruitment of HDACs.</P>

The sexual difference of costal cartilage

Yuan-Zhong Xie,Bo Mi Kim,Igic Sung Kim,Ok Hee Chai,Eui-Hyeog Han,Hyoung Tae Kim,Chang Ho Song(송창호) 대한체질인류학회 2013 대한체질인류학회 학술대회 연제 초록 Vol.56 No.-

Dynamic Mission-level Path Planning for Lunar Rovers

Xie Yuan,Zhou Jianliang,Wang Yong 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

Aimed at practical need of mission-level path planning for lunar rovers, a new environment modeling technology has been put forward, taking account of both lunar terrain features and solar energy effect. Besides traversability analysis of lunar terrain, a new solar energy assessment is computed considering both solar elevation angle and terrain features. Mobility and operation constraints of lunar rover are also considered in modeling the environment. As some factors of the environment varies regularly when traversing on the moon, a dynamic mission-level path searching method was developed. Environment is updated and path is re-planned at some frequency determined by the varying factor. The whole mission-level path from start to goal is a split joint of these path segments. The simulation results show that the synthesized environment model describes all the factors and their effects on lunar traverse properly; the dynamic path searching method implements mission-level path planning for lunar rovers under time-varying environments.

Involvement of EBV-encoded BART-miRNAs and Dysregulated Cellular miRNAs in Nasopharyngeal Carcinoma Genesis

Xie, Yuan-Jie,Long, Zhi-Feng,He, Xiu-Sheng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

The definite molecular mechanisms underlying the genesis of nasopharyngeal carcinomas (NPCs) remain to be completely elucidated. miRNAs are small non-coding RNAs which are implicated in cell proliferation, apoptosis, and even carcinogenesis through negatively regulating gene expression post-transcriptionally. EBV was the first human virus found to express miRNAs. EBV-encoded BART-miRNAs and dysregulated cellular miRNAs are involved in carcinogenesis of NPC by interfering in the expression of viral and host cell genes related to immune responses and perturbing signal pathways of proliferation, apoptosis, invasion, metastasis and even radio-chemo-therapy sensitivity. Additional studies on the roles of EBV-encoded miRNAs and cellular miRNAs will provide new insights concerning the complicated gene regulated network and shed light on novel strategies for the diagnosis, therapy and prognosis of NPC.

A Study of Feng Youlan's Aesthetic Thought in the Perspective of "Vital Beauty"

Yuan Xie KOREA INSTITUTE FOR HUMANITIES AND SOCIAL SCIENCES 2024 Journal of Arts and Cultural Studies(ACS) Vol.3 No.1

Background: Feng Youlan is one of the prominent figures in 20th-century Chinese philosophy, renowned for his contributions not only in the field of philosophy but also as an important aesthete. His ideas span various domains including philosophy, aesthetics, and social ethics, and are considered to have had a significant impact on traditional Chinese thought. Feng Youlan's aesthetic philosophy has garnered widespread attention and discussion in Chinese academic circles, marking it as a crucial milestone in the development of Chinese aesthetics in the 20th century. Purpose: The purpose of this paper is to delve into Feng Youlan's aesthetic philosophy and to analyze its significance and influence in the realm of aesthetics. Through a systematic examination of Feng Youlan's aesthetic ideas, a better understanding of the evolution, core concepts, and characteristics of traditional Chinese aesthetics can be achieved. Furthermore, an interpretation of his ideas can offer new insights and directions for contemporary aesthetic research. Methods: This paper employs a methodological approach involving literature review and analysis. By collecting, organizing, and analyzing relevant literature, it delves deeply into the core content and main viewpoints of Feng Youlan's aesthetic philosophy. Drawing on the research findings of other scholars, a comprehensive and thorough analysis and evaluation of Feng Youlan's "Vital Beauty" aesthetic ideas are conducted to ensure a comprehensive understanding and accurate grasp of his philosophy. Results: Feng Youlan's aesthetic philosophy encompasses various aspects, including discussions on the essence of beauty, standards of beauty, and the relationship between beauty, morality, and society. He posits that beauty not only possesses objective material attributes but also embodies a spiritual realm and intrinsic values. Feng Youlan advocates for the integration of aesthetics with morality and social ethics, emphasizing the practicality and value orientation of beauty in achieving the perfection of human existence. Conclusion: Feng Youlan's aesthetic philosophy has exerted a profound influence on traditional Chinese aesthetics, providing subsequent scholars with an important theoretical foundation and inspiration. Through an in-depth study and understanding of his ideas, the enrichment and development of traditional Chinese aesthetics can be facilitated, offering valuable insights and guidance for contemporary aesthetic research. Feng Youlan's aesthetic philosophy holds significant relevance in advancing the development and flourishing of Chinese aesthetics, and his intellectual legacy warrants deep contemplation and exploration.