Virtual Presentation and Customization of Products Based on Internet

Pan Zhi-geng,Chen Tian,Zhang Ming-min,Xu Bin Society for Computational Design and Engineering 2004 International Journal of CAD/CAM Vol.4 No.1

Through reviewing and comparing the current virtual shopping malls web sites integrated VR into E-commerce, this paper analyzed both the advantages and disadvantages of two kinds of methods for product presentation: 2D image based and 3D model based presentation method. Using the virtual shopping mall (EasyMall) as a showcase, we presented the architecture of the system and the development technologies, especially those in the mixed presentation method. The presentation and customization methods in the two related modules, including the PhoneShow for mobile phone and EasyShow for textile products, were discussed. It indicated that the integration of E-commerce with VR could provide consumers with virtual experience and intelligent service for business activities. Furthermore, the product presentation methods can be made available for use in different cases.

Molecular cloning of novel α-gliadin genes from Crithopsis delileana and the evolution analysis with those from Triticeae

Zhi-Fu Guo,Xiang-Yu Long,Pan Dong,Yu-Ming Wei,Li-Ping Bai,Xiao-Xuan Dang,Hao-Lei Wan,Li-Jun Zhang,You-Liang Zheng 한국유전학회 2011 Genes & Genomics Vol.33 No.2

The α-gliadins from Crithopsis delileana (Schult) Roshev (2n=2x=14, KK) were investigated by Acid polyacrylamide gel electrophoresis (A-PAGE) analysis. It was indicated that the electrophoresis mobility of gliadins from C.delileana had obvious difference with those from common wheat in α, γand ω region. Using primers designed from published sequences of α-gliadin genes, three α-gliadin genes were isolated from C. delileana, which were designated as gli-ka1,gli-ka2 and gli-ka3, respectively. Two in-frame stop codons were found in the coding sequences of gli-ka3, indicating that gli-ka3 could be a pseudogene. The gli-ka2 was a gliadin with an odd number of cysteines, resulting from a non-synonymous mutation. This change might lead to the interactive behavior of gli-ka2. Three α-gliadin genes of C. delileana had the similar but not identical primary structures to the corresponding gene sequences from other wheat related species. By the alignment of α-gliadin genes from Triticeae,phylogenetic analysis indicated that three α-gliadin genes of C. delileana clustered together with all α-gliadin genes from Ee genome of Lophopyrum elongatum by an interior paralleled branch.

Antioxidant Enzymes and Photosynthetic Responses to Drought Stress of Three Canna edulis Cultivars

Zhang, Wen-E,Wang, Fei,Pan, Xue-Jun,Tian, Zhi-Guo,Zhao, Xiu-Ming Korean Society of Horticultural Science 2013 원예과학기술지 Vol.31 No.6

Edible canna is a productive starch source in some tropical and semitropical regions. In these regions, water deficit stress is one of factors that limit the crop yield. In the present study, we investigated seven physiological indexes and photosynthetic responses of three edible canna (Canna edulis Ker.) cultivars ('PLRF', 'Xingyu-1', and 'Xingyu-2') under 35 days drought stress. Our results indicated that drought treatment caused visible wilting symptoms in all cultivars, especially in 'Xingyu-1'. Coupled with the increase of wilting symptoms, relative water content (RWC) and chlorophyll content decreased progressively, malondialdehyde (MDA) content gradually increased, and key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities increased first and then decreased in all three cultivars. The effect of water stress was more pronounced in 'Xingyu-1' than in 'PLRF' and 'Xingyu-2', and in lower leaves than in upper leaves. In addition, 35 days drought stress also significantly reduced the photosynthetic capacity. Consistent with antioxidant parameters, photosynthetic changes of 'Xingyu-2' were less than those of the other cultivars under water deficit stress. Drought stress caused a significant increase of water use efficiency (WUE) in 'Xingyu-2', but little in 'PLRF', and obvious decrease in 'Xingyu-1'. These results indicated that 'Xingyu-2' was more tolerant to drought stress than 'PLRF' and 'Xingyu-1' by maintaining lower lipid peroxidation and higher antioxidant enzyme activities.

Suppression of CDK2 expression by siRNA induces cell cycle arrest and cell proliferation inhibition in human cancer cells

( Xiang E Long ),( Zhao Hui Gong ),( Lin Pan ),( Zhi Wei Zhong ),( Yan Ping Le ),( Qiong Liu ),( Jun Ming Guo ),( Jiu Chang Zhong ) 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.4

Cyclin-dependent kinase 2 (CDK2) is a member of serine/threonine protein kinases, which initiates the principal transitions of the eukaryotic cell cycle and is a promising target for cancer therapy. The present study was designed to inhibit cdk2 gene expression to induce cell cycle arrest and cell proliferation suppression. Here, we constructed a series of RNA interference (RNAi) plasmids which can successfully express small interference RNA (siRNA) in the transfected human cells. The results showed that the RNAi plasmids containing the coding sequences for siRNAs down-regulated the cdk2 gene expression in human cancer cells at the mRNA and the protein levels. Furthermore, we found that the cell cycle was arrested at G0G1 phases and the cell proliferation was inhibited by different siRNAs. These results demonstrate that suppression of CDK2 activity by RNAi may be an effective strategy for gene therapy in human cancers. [BMB reports 2010; 43(4): 291-296]

Antioxidant Enzymes and Photosynthetic Responses to Drought Stress of Three Canna edulis Cultivars

Wen-E Zhang,Fei Wang,Xue-jun Pan,Zhi-guo Tian,Xiu-ming Zhao 한국원예학회 2013 원예과학기술지 Vol.31 No.6

Edible canna is a productive starch source in some tropical and semitropical regions. In these regions, water deficit stress is one of factors that limit the crop yield. In the present study, we investigated seven physiological indexes and photosynthetic responses of three edible canna (Canna edulis Ker.) cultivars (‘PLRF’, ‘Xingyu-1’, and ‘Xingyu-2’) under 35 days drought stress. Our results indicated that drought treatment caused visible wilting symptoms in all cultivars, especially in ‘Xingyu-1’. Coupled with the increase of wilting symptoms, relative water content (RWC) and chlorophyll content decreased progressively, malondialdehyde (MDA) content gradually increased, and key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities increased first and then decreased in all three cultivars. The effect of water stress was more pronounced in ‘Xingyu-1’ than in ‘PLRF’ and ‘Xingyu-2’, and in lower leaves than in upper leaves. In addition, 35 days drought stress also significantly reduced the photosynthetic capacity. Consistent with antioxidant parameters, photosynthetic changes of ‘Xingyu-2’ were less than those of the other cultivars under water deficit stress. Drought stress caused a significant increase of water use efficiency (WUE) in ‘Xingyu-2’, but little in ‘PLRF’, and obvious decrease in ‘Xingyu-1’. These results indicated that ‘Xingyu-2’ was more tolerant to drought stress than ‘PLRF’ and ‘Xingyu-1’ by maintaining lower lipid peroxidation and higher antioxidant enzyme activities.

Fifth Generation Communication Performance of Poly(ether ketone ketone)/Modified Montmorillonite Substrate

Guang Kui Zhou,Xiao Dong Zhi,Deng Ming Pan,Tim Hsu,Jen-taut Yeh 한국고분자학회 2022 Macromolecular Research Vol.30 No.2

Quaternary ammonium salt modified montmorillonite (QASMMT) nanoplatelets were well dispersed in poly(ether ketone ketone) (PEKK) polymers to make pleasing fifth generation (5G) substrates. Substantially smaller dielectric properties and linear thermal expansion coefficient (LCTE) were found for each PEKKa xQASMMTy film series filled with small and appropriate QASMMT loadings. The dielectric constant (εr) and dielectric loss (tan δ) values acquired for each PEKKa xQASMMTy film series reduced to a lowest value as QASMMT loadings approached 3wt%. Satisfactory εr (2.59, 2.71, and 2.80 at 1 MHz), tan δ (0.0032, 0.0038, and 0.0042 at 1 MHz) and LCTE (~36.8×10-6/℃, 38.8×10-6/℃ and 40.5×10-6/℃) for 5G communication were acquired for PEKKa 97QASMMT3 films filled with only 3wt% optimum loading of QASMMT nano-platelets. In the meantime, the onset degradation temperatures acquired for each PEKKa xQASMMTy film series increased substantially with increasing QASMMT loadings. All free volume properties, such as, the radius of the free-volume-cavity or free-volume-cavity numbers per unit volume evaluated for every PEKKa xQASMMTy film sequence enlarged to a largest value, as QASMMT loadings approached an appropriate value of 3wt%. Substantial smaller εr and tan δ were acquired for PEKKa and PEKKa xQASMMTy with larger free volume properties. Possible reasons accounting for these substantially diminished dielectric and LCTE properties of PEKKa xQASMMTy films are proposed.

Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy

Zhou Jing,Feng Ji,Wu Yong,Dai Hui-Qi,Zhu Guang-Zhi,Chen Pan-Hong,Wang Li-Ming,Lu Guang,Liao Xi-Wen,Lu Pei-Zhi,Su Wen-Jing,Hooi Shing Chuan,Ye Xin-Pin,Shen Han-Ming,Peng Tao,Lu Guo-Dong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.

Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

( Yuan Qing Hu ),( Jin Lin Huang ),( Qiu Chun Li ),( Yu Wei Shang ),( Fang Zhe Ren ),( Yang Jiao ),( Zhi Cheng Liu ),( Zhi Ming Pan ),( Xin An Jiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.