전격성 경과를 취한 만성 호산구성 폐렴 환자 1예

윤호상,진춘조,유광하,이상엽,이수인,정상만,김선두,이순제,이길도,전혜정 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Chronic eosinophilic pneumonia(CEP) is a rare disease of unknown etiology characterized by chronic infiltration of the lung with eosinophils. It presents with profound systemic symptoms comprising fever, night sweats, weight loss, dyspnea and blood eosinophilia with nonsegmental air-space consolidation confined to the outer third of the lung, the "photographic negative of pulmonary edema". Histopathologic features of chronic eosinophilic pneumonia are intraalveolar and interstitial infiltrations with eosinophils, histiocytes, giant cells, scattered lymphocytes and plasma cells. Glucocorticoid therapy cause prompt resolution of symptoms as well as disappearans of blood eosinophils, elevated serum Ig E levels and the roentgenographic lesions. Infrequent radiographic findings include nodular infiltrates, consolidations, cavitations, atelectasis and pleural effusions. Deaths from CEP although rare, have been reported, but the majorities of CEP have benign courses and do not need a therapy with ventilator. We report a case of chronic eosinophilic pneumonia, which had a sudden course associated with diffuse pneumonic consolidations on the both lung and bilateral pleural effusion.

금은화 약침액의 암세포 성장 저해 효과

손윤희,임종국,최혜경,남경수,배만종 경산대학교 생명자원개발연구소 1999 생명자원과 산업 Vol.3 No.-

한방에서 염증, 종양 억제 등에 사용되는 금은화로 약침액을 조제하여 쥐와 사람의 암세포 성장억제효과를 살펴 보았을 때 금은화 약침액 10×, 5×농도는 mouse EATC에 대해 90% 및 86% 세포 성장 억제율이 있었으며, 열수추출액 10×, 5× 농도는 89% 및 87% 세포 성장억제효과가 있었다. 같은 농도의 약침액을 Hepalclc7에 처리했을 때는 89.5%, 69.7%의 억제효과가 있었고 열수추출액은 85.7%, 67.3%의 hepalclc7 세포 성장억제율을 보였다. 사람의 암세포주 A549, HeLa 세포에 대한 금은화 약침액 및 열수추출액의 세포독성 실험에서도 A549 세포에 약침액 10×, 5×를 처리했을 때 82.0%, 46.1% 세포 성장억제효과가 나타났으며 금은화 역수추출액은 75%, 50%의 세포 성장 억제율이 나타났다. HeLa 세포에서는 약침액이 열수추출액보다 세포 성장 억제효과가 더 높았다. 금은화 약침액을 처리한 세포(EATC,Hepalclc7과 human A549, HeLa)의 현미경 관찰에서는 세포수의 감소와 형태의 변화를 관찰할 수 있었다. Lonicerae flos Aqua-acupuncture Solution(LFAS) and Lonicerae flos Water-extracted Solution(LFWS) were prepared and tested for their potential antitumor activities. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of LFAS at 10× and 5× resulted in more than 50% inhibition of growth in Ehrich ascites tumor cells(EATC), Hepalclc7, and HeLa cells after 72 hours. Toxicity of LFWS to A549 revealed that more than 50% inhibition of growth at LFAS conentration of 10× after 72 hours. Concentration of LFWS at 10× and 5× showed more than 50% inhibition of growth with EATC, Hepalclc7, HeLa and A549. In morphological study, the number of cells were decreased, and the shape of cells was round form in EATC, Hepalclc7, HeLa, and A549.

동물과 사람유래 Vancomycin 내성 장구균의 항균제 감수성 비교

조윤상,이희수,김종만,류판동,박용호,유한상,이문한 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

It has been recently reported the possibility in the bansfer of antimicrobial resistance to other animals and humans. In particular, vancomycin resistant enterococci (VRE), which have been known as a principal antimicrobial resistant bacteria in humans, have been increased as a pathogen of nosocomial infections. And then animal VRE were suspected as an origin of human VRE. In this study, we isolated Enterococcus spp. from animals, identified by bio- chemical tests, examined for antimicrobial susceptibility, and then compared the antimicrobial susceptibility of VRE among each other as well as human VRE. Enterococcus iecium (29%) was predominant in Enterococcus species (n=122) isolated from animal feces in this study. E. hirae, E. iecalis, E. casseliflam and E. gallinarum were also isolated as rates of 24%, 21%, 16% and 7%, respectively. The resistance of enterococci to penicillin and tetracycline were 66% and 78%, respectively, and the susceptibility of them to chloramphenicol was 66%. Antimicrobial susceptibility test has shown that 91% of VRE from humans (n=11) was susceptible to chloramphenicol and all resistant to penicillin, rifampin and streptomycin. Seventy-five percentage of VRE from chickens (n = 12) was susceptible to rifampin and resistances of them to penicillin, tetracycline and sbeptomycin were 75%, 83%, and l00%, respectively. Therefore, we confirmed the difference of antimicrobial susceptibility from animals and humans, and the antimicrobial susceptibility test could be one of the simple and useful methods for the epidemiological survey of VRE.

Random Amplified Polymorphic DNA Typing에 의한 동물과 사람 유래 Vancomycin 내성 장구균의 유전학적 비교

조윤상,이희수,김종만,류판동,박용호,유한상,이문한 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.1

It has been recently reported the possibility in the transfer of antimicrobial resistant to other animals and humans. In particular, the occurrence of vancomycin resistant enterococci (VRE), which have been known as a principal antimicrobial resistant bacteria in humans, has been increased as a pathogen of nosocomial infections. And then animal VRE were suspected as an origin of human VRE. In this work, we investigated the relatedness between VRE from animals and humans through random amplified polymorphic DNA (RAPD) typing. The genetic relatedness of vanA, vanB, vanC-1 and vanC-2 genotypes was examined by RAPD typing, respectively. van4 genotypes have not been shown high genetic relationship each other, containing VRE from humans (n = 8) and chickens (n = 5). In vanB genotypes from humans (n = 3), 2 isolates were made up one cluster, shown 77.8% homology. Chicken isolates of vanC-1 genotypes (n = 11) were constituted 2 clusters and the homology of 2 clusters was 81.8% and 80.0%, respectively. In case of vanC-2 genotypes (n = 19), 2 isolates from pigs have been shown 76.9% homology. But, between VRE from animals and humans, the isolates of high genetic relationship could not be found and RAPD typing was a useful epidemiological method that could be confirmed the genetic relationship among VRE.

저온유통용 골판지 포장상사 개발에 관한 연구 (Ⅰ) : 골판지의 흡습특성 시험 Characteristics of moisture absorption to corrugated fiberboard

이원옥,윤홍선,정훈,이현동,조광환,김만수 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

농산물의 저온저장 및 고습조건에서 안전하게 유통할 수 있는 골판지 포장상자를 개발하기 위하여 원지의 구성, 라이너의 발수도, 골형태의 차이에 따라 수분흡습 특성과 압축강도와의 관계를 구명하기 위해 각각 원지특성을 달리하여 이중 양면 골판지를 재료로 사용하여 시험하였다. 온도변화에 따른 골판지 함수율 변화는 골판지 원지 종류, 발수도 차이등에 관계없이 거의 일정한 값을 나타냈다. 상대습도 변화에 따른 골판지의 함수율 변화는 상대습도에 크게 영향을 받는 것으로 나타났으며 골판지의 수분흡습은 발수도 보다는 원지에 구서에 따라 영향을 많이 받는 것으로 나타났다. 또한 수직압축강도는 상대습도가 증가함을 따라 크게 감소하여 상대습도가 수직압축강도에 미치는 영향이 매우 크며 상대습도가 증가함에 따른 수직압축강도의 저하비율은 골판지의 종류에 관계없이 거의 같은 것으로 나타났다. 따라서 저온고습 조건에서 안전하게 내용물을 보호할 수 있는 저온유통용 농산물 골판지 상자의 개선을 위해서는 고품질 원지의 생산기술 개발 및 골판지 상자의 구조개선 연구가 우선되어야 할 것으로 판단된다. The hardness of corrugated packaging box decreased during storage period because of high humidity. Therefore, the strength-decreasing factor of corrugated packaging box had to be investigated to determine the compression strength of the corrugated packaging box. This study was conducted to define the characteristics of moisture absorption as well as compression strength of corrugated fiberboard. (1) The moisture content of the corrugated fiberboard was rarely influenced by the kinds of raw materials and water repellent. However, the moisture adsorption of the corrugated fiberboard was shown to be clearly affected by air temperature changes. (2) The moisture content of the corrugated fiberboard was significantly affected by relative humidity. The moisture adsorption of corrugated fiberboard was also greatly influenced by composition of raw materials rather than water repellent. The results indicated that the improvement of raw materials was more effective than the increase of water repellent of corrugated fiberboard to reduce the moisture adsorption of the corrugated fiberboard. (3) The vertical compression strength was significantly decreased with increasing relative humidity. The reduction rate of vertical compression strength was not charged with the increase of relative humidity, which was regardless of kinds of corrugated fiberboard. (4) For improving the strength of the corrugated packaging box, new technique should be found to develop better quality of raw materials. Moreover, the physical construction of corrugated packaging box should be clearly understood because the corrugated packaging box for the agricultural products was usually used for the circumstances of high humidity and low air temperature.

耕耘實驗을 爲한 人工土壤의 物理的 特性에 關한 硏究

金基大,許潤根,金滿秀,金聲來 충남대학교 농업과학연구소 1978 農業技術硏究報告 Vol.5 No.2

For improvement and new design of tillage equipments, indoor test is very useful and more desirable than outdoor because the experiment of ourdoor is very difficult and its cost is expensive. This study was carried out to determine the physical properties of artificial soil suitable for the indoor test with the soil bin manufactured at the workshop of the Dept. of Agricultural Machinery Engineering. The artificial soil being studied was made with very similarity to the natural soil of the experimental plots of Chungnam National University, and it consist of 39.35 percent, by weight of bentonite and 48.10 percent of sand with 12.55 percent of SAE 10W oil. The results are summarized as follows: 1. Bulk density increased with increasing number of rolling, and its relationship could be expressed. y=1.073200+0.07080x-0.002263x^2 where, y=bulk density(g/㎤), x=number of rolling. These results could be explained that the effect of rolling velocity on the bulk density was not singnificant in the range of 4.5∼10.4 cm/sec. 2. The absolute soil hardness depended directly upon number of rolling, and their relationship could be expressed by the equation. y=37.74(0.64+0.17x-0.0054x^2)/(3.36-0.17x-0.0054x^2)^3. where, y=absolute soil hardness(kg/㎤), x=number of rolling. 3. Relationship between the bulk density and absolute soil hardness could be expressed by the equation; y=37.74(2.46 x-2.02)/(6.02-2.46x)^3. where, y=absolute soil hardness, x=bulk density. 4. The cohesion and the angle of internal friction of artificial soil were increased with increasing its bulk density. According to the cohesion and angle of internal friction, at the range of 1.60∼1.75(g/㎤) of bulk density, this artificial soil was similar with sandy loam of 29.5% moisture content of natural soil. 5. Sliding-fricfion coefficient of steel plate on the artificial soil was 0.3∼0.4 and rubber plate on it is 0.64∼0.72. Those values were very similar with those of natural soil being studies by many others.

열 변화에 의한 구리전선의 미세조직 및 상 변화에 관한 연구(Ⅲ)

박성우,김윤회,김상현,박남규,김수철,김영만 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

600합금의 미세조직 변화 및 SCC거동에 미치는 Ce 첨가의 영향

권오철,이용선,임연수,정만교,김정수 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.1

High purity model alloys with major composition Ni-15Cr-9Fe-0.03C (600CEO) and Ni-15Cr-9Fe-0.03C-0.04Ce (600CE4) were produced. Using these model alloys the effect of alloying element Ce on the SCC behavior of Alloy 600 was evaluated in a caustic solution. To obtain carbides precipitated on grain boundaries, the thermal treatment was performed on both the solution annealed model alloys. Microstructural examinations using SEM and TEM EDX showed that the same structural carbides, Cr_(7)Cc₃, were precipitated on both the alloys and no significant difference in the amount of Cr depletion along grain boundaries was observed between the two model alloys. However, it was shown that the coverage of grain boundary carbides was higher in the Ce-bearing alloy (600CE4). The SCC susceptibility of the alloys was investigated in 40%NaOH solution at 315℃. Being evaluated in terms of the maximum crack depth, the SCC susceptibility of the alloy turned out to be reduced by the addition of Ce. The increased resistance to the SCC in the alloy 600CE4 was considered to be attributable to the increased coverage of grain boundary carbides. (Received September 30, 2004)

동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

한국어 대용량 음성인식에서의 효율적인 시간 감축에 관한 연구

장춘서,이기수,신윤식,임은기,김병만,김현수 금오공과대학교 산업기술개발연구원 2000 産業技術開發硏究 Vol.16 No.-

In this paper a time reduction algorithm for large vocabulary speech recognition system has been studied. TDNN(Time Delay Neural Network) was used to classify vowel using phonetical fetures. To increase performance of vowel classification, modular TDNN architecture associated with 2 states input layer has been suggested.