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비교 피내 검사와 감마 인터페론 검사에 의한 우결핵의 진단
조윤상,김종만,정석찬,우승룡,김종염,유한상,박용호,안종삼,Cho, Yun-sang,Kim, Jong-man,Jung, Suk-chan,Woo, Seung-ryong,Kim, Jong-yeom,Yoo, Han-sang,Park, Yong-ho,Ahn, Jong-sam 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6
Since Robert Koch found tubercle bacilli in 1882, the studies on tubercle bacilli of human and animal had been carried out. Being old tuberculin(OT) introduced in 1890, the specificity of the diagnosis of tuberculosis has been improved by continual uses of heat concentrated synthetic medium(HCSM) and purified protein derivatives(PPD) tuberculin. Now, two types of tuberculin test are used worldwidly ; the single intradermal test(SIT) using bovine tuberculin and the single intradermal comparative tuberculin test(SICTT) using avian and bovine tuberculins. In the SICTT, each countries have used with different combination of both avian and bovine tuberculins' titers. However, this kinds of studies have not reported in Korea. Therefore, the studies on the combination of their tuberculins' titers were performed through intradermal test of guinea pigs sensitized with either Mycobacterium bovis or M avium and were examined in 10 cattles of SIT positive reactors. Also, IFN-${\gamma}$ assay, the latest diagnostic method of bovine tuberculosis, was experimentally applied to SIT positive reactors. For determining the optimal titers, sensitized guinea pigs with M bovis and M avium were intradermally injected avian and bovine tuberculin. In guinea pigs sensitized with M bovis, bovine tuberculin 50 T.U. showed significant difference from all tested concentrations of avian tuberculin(p < 0.05). In guinea pigs sensitized with M avium, there is significantly different between bovine tuberculin and avian tuberculin by 25 T.U.(p < 0.01). Therefore, optimal titers of bovine and avian PPD tuberculins' titers for the SICTT in Korea were 5,000 and 2,500 tuberculin units, respectively, and the swelling diffences between bovine and avian site in SIT positive reactors were above 3mm. Also, in IFN-${\gamma}$ assay, the 9 SIT positive reactors were showed all the positive reactions.
방향족 활성 Chloro화합물의 Alkoxy기 치환에 관한 연구
조윤상,공영식 대한약학회 1975 약학회지 Vol.19 No.2
p-Chloronitrobenzene(substrate) and p-nitrophenetole (product) were quantitatively analyzed to know the degree of extent of reaction in the process of time. The calibration curve was prepared by the internal satndard method in gaschromatography. 2,6-Dimethyl-naphthalene was used as internal standard. The rate constant(k), the reaction velocity in various concentrations of NaOH altered, and the formation of byproducts(azo-compound and p-nitrophenol) with the amounts of MnO$_{2}$ and NaOH altered, were studied. From the results of these of MnO$_{2}$ and NaOH altered, were studied. From the results of these experiments, this reaction was second order and the rate constant was k=10.3 $\times$ 10$^{-3}$ mole$^{-21$. When p-chloronitrobenaene 1 pt. NaOH 0.56pts. MnO$_{2}$ 0.5pts. and ethanol 25 pts-were reacted about 10 hours, p-nitrophenetole was nearly quantitatively obtained without byproducts.
L(+)-Glutamic Acid 유도체의 합성(II)
조윤상 대한약학회 1979 약학회지 Vol.23 No.2
In the formation of L(+) -glutamine from L(+) -glutamic acid-5-hydrazide, large amount of Raney-Ni was effective under normal pressure but hydrogenation or amonolysis of ester under pressure was useless. Preparation of glutamine with .alpha.-ketoglutaric acid (by way of 1, 4, 5, 6-tetrahydro-6-oxo-3-pyridazine carboxylic acid) is intersting but not so efficient in yield.
장구균의 vancomycin 내성 유전자와 종 특이유전자의 검출을 위한 Multiplex polymerase chain reaction 개발
조윤상,이희수,김종만,안종삼,류판동,박용호,유한상,이문한,Cho, Yun-Sang,Lee, Hee-Soo,Kim, Jong-Man,Ahn, Jong-Sam,Ryu, Pan-Dong,Park, Yong-Ho,Yoo, Han-Sang,Lee, Mun-Han 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1
A multiplex PCR assay, which allows simultaneous detection of vancomycin resistant genotypes and Enterococcus species-specific genes, was developed. Vancomycin resistant enterococci (VRE) from chickens and humans could be detected for vanA, vanB, vanC-1, vanC-2, $ddl_{E.faecium}$ and $ddl_{E.faecalis}$ by multiplex PCR. Eight isolates of VRE from humans (n=11) had $ddl_{E.faecium}$ and vanA, and 3 isolates of the VRE had $ddl_{E.faecium}$ and vanB. One isolate of VRE from chickens (n=6) had $ddl_{E.faecium}$ and vanA, and 5 isolates of the VRE had only vanA. E. faecium, E. faecalis, E. gallinarum and E. casseliflavus were also confirmed for the species-specific gene by multiplex PCR. This multiplex PCR could detect E. faecium, E. faecalis, E. gallinarum, E. casseliflavus, vanA, vanB, vanC-1 and vanC-2, simultaneously. The PCR assay established in the present study can be an alternative to time-consuming biochemical tests and antibiotic susceptibility tests of Enterococcus spp.
결핵 진단액(Purified Protein Derivative)의 단백질체 분석
조윤상 한국보건교육건강증진학회 2005 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-
상당히 성공적인 사람병원체인 결핵균을 Robert Koch가 발견한 이래, 결핵 방제의 상당한 진보에도 불구하고, 결핵의 발생은 현재까지 계속되어오고 있다(Tiruviluamala and Reichman 2002). 그러므로, 완전한 민감성과 특이성을 갖는 신속한 진단방법이 결핵과 결핵의 잠복감염을 진단하는데 요구되어지며(Tiruviluamala and Reichman 2002), 계속적으로 결핵균과 BCG의 유전체와 단백질체 연구를 통하여 결핵 방제수단이 발전되어질 것이다(Jungblut et al. 1999). 본 연구는 PPD가 결핵을 진단하는 특이한 항원만으로 정확하게 조화를 이루고 있지 않다는 것을 보여주었으며, 그것의 역가는 결론적으로 생물학적으로 평가되어져야 하지만, PPD의 제조는 단백질체 분석 기법에 의해 분석되고 표준화될 수 있음을 보여주었다. 이를 통하여 결핵 피내진단의 일관된 결과를 위해 PPD 사용 전에 이와같은 단백질체 분석기법을 적용할 수 있을 것으로 사료되며, 본 연구에서 밝혀진 특이 항원은 새로운 피내진단항원으로 적용될 수 있을 것으로 사료된다.
조윤상 한국과학학술지편집인협의회 2022 Science Editing Vol.9 No.2
There are many ways to use open source code to implement digital standards for scholarly journal publishing. However, providing digital services using open-source code can be a challenge, especially for small and local academic society journals. This paper provides some critical examples of using some of the many open-source code resources available to the public. Journal Article Tag Suite (JATS) Extensible Markup Language (XML) has been established as an essential tool, and is now used by most journals for digital publication. JATS XML can be converted to other viewer formats, including Extensible Hypertext Markup Language, PubReader, and EPUB 3.0. It can also be used to create dynamic interactive PDFs. It can be converted to other XMLs, incluing Crossref XML, PubMed XML, and DOAJ XML. Open-source code published on GitHub, National Information Standards Organization, and the US National Library of Medicine can be used for Crossref XML deposition for digital object identifier and Crossmark stamp registration. These examples of open-source code need to be implemented on journal websites to provide local academic journal publishers with various critical functions. This paper provides instructions on the best ways to realize these digital standards so that journal content can be provided to readers in a more friendly and effective way.