Rhizopus microsporus 에서의 protoplasts 형성의 최적 조건

민병례,박유정 상명대학교 자연과학연구소 2002 自然科學硏究 Vol.9 No.-

Rhizopus microsporus var. oligosporus (ATCC 22959)의 spore로부터 원형질체 생성을 유도할 수 있는 최적 조건들을 연구하였다. 2-deoxy-D-glucose가 첨가된 액체배지에서 7시간 swelling 시킨 spore로부터 최대한의 protoplast 생성을 유도할 수 있었으며, 세포벽 분해효소 처리 3시간 이후에 원형질체 생성이 최대치에 도달하였다. 0.5M glucose를 삼투안정제로 이용하였다. Novozym 234, β-glucuronidase와 cellulase의 세포벽 분해 효소중에서 Novozym 234(6㎎/㎖)와 β-glucuronidase(5000unit/㎖)의 mixture가 가장 우수한 것으로 판명되었다. Optimal conditions for the protoplasts formation from spore of Rhizopus microsporus var. oligosporus (ATCC22959) were studied. A large number of protoplast were obtained by swelling of spore on liquid medium adding 2-deoxy-D-glucose for 7hrs, and by reaction with cell wall lytic enzyme for 3hrs. 0.5M glucose was used as the osmotic stabilizer. Among effective cell wall lytic enzymes, Novozym 234, β-glucuronidase and cellulase, mixture of Novozym 234(6㎎/㎖) and β-glucuronidase(5000unit/㎖) was the most effective one on the production of protoplasts.

고정성 Resin-bonded bridge를 이용한 상실된 유전치부의 심미 수복 : 증례보고 Case reports

박인천,박성규,위유민,이봉규,김효석,이상호 조선대학교 구강생물학연구소 2002 口腔生物學硏究 Vol.26 No.2

Today's parents are quite conscious of the esthetic and Potential psychological consequences of missing anterior teeth. Premature lobs of Primary incisors usually dose not require the Placement appliances for space maintenance because no mesial movement of the adjacent teeth is normally expected when the canines have already erupted. However, replacement of anterior teeth may be indicated for esthetic purposes or possibly to facilitate normal pronunciation. The treatment options in these cases are removable maintainers of space or fixed partial dentures, the former being most often used. When no cooperation on the part of the patient for their used or when the devices provoke discomfort or atypical glutition, fixed partial dentures may be used, taking into consideration premaxillary growth.

성격의 변화를 수반한 Wilson씨병 1례

유세종,이상렬,박민철 圓光大學校 醫科大學 神經精神科學敎室 1994 圓光精神醫學 Vol.10 No.2

We had a 22 year old male patient with Wilson's disease showing neuropsychiatric symptoms. He showed neuropsychiatric symptoms such as personality change, regressed and childish behavior, wing-beating tremor and rigidity on the upper extrmity, open mouth, drooling, bradykinesia, dysarthria and dysphagia. In brain MRI findings, bilateral high signal intensity on T2WIs were observed in basal ganglia, thalamus, central midbrain and periaqueductal pons. In liver scan, splenomegaly was detected. At a slit-lamp examination, we detected the brownish colored pigments(Kayser-Fleisher ring) on Descement's membrain of both eye corneas. Laboratory findings were a low serum ceruloplasmin(2.63㎎/dl, normal: 25~45㎎/dl), low serum copper level(32㎍/dl, normal: 80~130㎍/dl), and an increased 24 hous urine-copper excretion level(258㎍/day, normal: 15~30㎍/day). Therefore, we diagnose the patient as having wilson's disease and we started medication treatment with penicillamine.

성인 지역사회 폐렴의 원인 미생물에 대한 전향적 다기관 연구 : Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi

김민자,정희진,손장욱,심희선,박대원,박승철,우준희,강재명,김유겸,신완식,김양리,이환종,김지희 대한감염학회 2001 감염 Vol.33 No.1

Background : Despite rigorous investigations, the etiology of community-acquired pneumonia remains unknown in about 50% of hospitalized patients. The diagnosis of the etiological agent is becoming more challenging and more critical as number of newer pathogens have been recognized in recent years. In the 3-year period prospective study we investigated adult patients with community-acquired pneumonia for Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi as potential etiologic agents. Methods : A prospective multicenter study was performed from May 1997 to April 2000. A total of 431 patients with community-acquired pneumonia under the inclusion criteria were examined for specific microbial diagnosis; sputum culture and PCR, and serologic teats including indirect immunofluorescence antibody (IFA) test for Legionella, and hemagglutination tests for Leptosoira, Hantaan virus and O. tsutsugamushi. Etiologic diagnosis was determined on the basis of the review of case record forms and specific laboratory diagnostic criteria. Results : During the study period a total of 385 sputum and 283 serum samples were examined. Legionella pneumonia was diagnosed in 2.3% (10/431) of the cases examined : 1.4% cases with PCR-positive (5/367) and 2.1% with positive IFA test (6/283). Leptospirosis and scrub typhus were diagnosed in 0.4% (1/252) and 2.0% (5/252), respectively. All 5 cases with scrub typhus occurred in late fall, and rash or eschar was not found. None of cases was Hantaan virus infection. Conclusion : The results suggest that Legionella, Leptospira, and O. tsutsugamushi should be considered in the etiologic diagnosis and empirical antibiotic therapy of community-acquired pneumonia. (Korean J Infect Dis 32:24∼31, 2001)

N-Arylnortropinone 유도체의 합성연구

李龍均,朴유미,鄭大一,金寅植,朴鍾勳,盧信愛,鄭逸守,朴敏洙 東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.1

Tropinone 화합물들은 색다른 고리계의 존재로 아주 오래전부터 약리학적으로 매우 흥미로운 연구과제로 주목되어 최근에 그들의 합성법,?? 입체적 구조분석?? 그리고 약리학적인 연구??가 활발히 진행되고 있다. Tropinone 1은 흔히 마약으로 알려져 있는 cocaine 2, 소염작용과 항경련성 작용 및 심장박동수를 조절 등 다양한 약리작용을 하는 tropane spirohy-dantoin ?? 그리고 안과질환을 검사 할 때 눈의 동공을 확장시키는 데 사용하는 atropine ??등을 합성하는 데 중요한 중간체이다. ??????????? Trigo, G, G등 ??은 aliphatic amine과 succinic aldehyde를 acetonedicarboxylic acid와 반응하여 nortropane 유도체를 합성하였으며, 그 후 [3+2] nitrone cycloaddition? 과 [3+4] cyclocoupling? 을 거치는 tropane alkaloid의 새로운 합성법이 개발되었다. 최근 lzquier M. L. 등¹은 acetonedicarboxylic acid를 사용하여 1차 지방족 amine과 succinic aldehyde대신 2,5-dimethoxytetrahydrofuran을 반응시켜 N-alkyl nortropinone 유도체를 합성하였다. 그러나 일반적으로 빙초산 속에서 amine과 2,5-dimethoxytetrahydrofuran을 반응시키면 pyrrole 유도체가 합성되어진다.?? 이러한 사실은 분명히 반응조건(예, 산, 온도)에 따른 다양한 화학이 존재한다고 볼 수 있다. 따라서 우리는 반응조건, 즉 산의 변화 및 온도 변화에 의한 amine과 2,5-dimethoxytetrahydrofuran의 반응을 자세하게 검토하면서 새로운 N-arylnortropinone 유도체를 합성하고자 한다.

전산화단층사진을 이용한 타액선의 정량분석에 관한 연구

최순철,박태원,이상철,유동수,허민석,이삼선 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: The purpose of this study was to calculate the size and CT number of both normal parotid and submandibular gland, and evaluate their relation to sex, age and obesity using computed tomography. Materials and Methods: The computed tomography was performed parallel to the Frankfurt plane in 46 subjects with healthy salivary gland. The subjects were divided into the three groups(young, middle, old) according to their ages. The size of salivary gland was determined as maximum cross-sectional area and the CT number of salivary gland was determined as the mean CT number of three ROI's. The body mass index was calculated from weight and height. Results: The mean maximum cross-sectional area was 7.79(±1.25) ㎠ on parotid gland and 4.12(±0.83) ㎠ on submandibular gland. The mean CT number was -4.43(±23.87) HU on parotid gland and 50.01(±15.63) HU on submandibular gland. There was decreasing pattern of the maximum cross-sectional area of submandibular gland and the CT number of both parotid and submandibular gland according to age(p<0.50). As the body mass index increased, the maximum cross-sectional area of parotid gland increased and CT number of both parotid and submandibular gland decreased(p<0.05). The maximum cross-sectional area of submandibular gland in male was larger than that in female(p<0.05). As the maximum cross-sectional area and CT number of left salivary gland increased, those of right gland increased(p<0.05). Conclusion: Intra-individual differences in salivary gland size and CT number is considered in the age and individual obesity.(J Korean Oral Maxillofac Radiol 1999:29:207-219)

기능적 방법을 이용한 소아의 하악과두 골절의 처치 : 증례 보고 A case report

이정하,박성규,위유민,정현구,이봉규,이창섭 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2

The pediatric condylar fracture occurs very frequently in the mandible, but this injury is occasionally ignored due to difficulty of diagnosis and no cooperation of patient. The adequate initial diagnosis and active treatment must be performed because delayed and improper treatment lead to possible severe complication such as TMJ ankylosis and retardation of mandibular development. Most pediatric condylar fracture is mainly performed by the conservative or functional treatment. but it may be required open reduction according to cases. In this study, Activator is applied for functional treatment of pediatric condylar fracture and fair recovery of jaw function is acquired.

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Youn, Cha Kyung,Park, Seon Joo,Li, Mei Hong,Lee, Min Young,Lee, Kun Yeong,Cha, Man Jin,Kim, Ok Hyeun,You, Ho Jin,Chang, In Youp,Yoon, Sang Pil,Jeon, Young Jin The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-${\alpha}$, IFN-${\gamma}$, and IL-$1{\beta}$). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-${\kappa}B$/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

견비통의 한·양방 진료 및 협진의 경제성 평가

장혜정,홍상민,박유선,남동우,이두익,이재동,이윤호,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : The purpose of this study was to evaluate and compare the cost-effectiveness of Eastern, Western and collaborative treatments and suggest a cost-effective approach for patients with frozen shoulder pain. Methods : Using the data of fifty-two patients, treatment effectiveness was measured by CSA, SPADI, and ROM scales and changes from the baseline score were evaluated. Data source for cost estimation was based on the national health insurance (NHI) payment system. Because the price in NHI was differentiated by health care institutions, five collaborative types were considered in assessing costs. Cost-effective ratios were computed tor economic evaluation. Results : Compared with Eastem treatinent, collaborative and Westerm treatments showed better effects on CSA scale after 4 weeks' treatment. The collaborative approach was also the most effective treatment on SPADI and ROM scales The direct cost per patient receiving Eastern treatment was less than other treatments. In general, collaborative treatment dominated Eastern and Western treatments in cost-effectiveness analysis However, the cost-effectiveness ratio of Eastern treatment resulted in \9,0OO compared to W29,OOO of collaborative treatment on SPADI Four different indicators of ROM scales resulted in different approaches as the cost-effective treatment. Conclusiuns : Considering cost-effectiveness ratios, collaborative treatment was the best treatment on CSA and SPADl scales after 4 weeks' treatment As for ROM scales, the recommended altematives were Eastern treatment for patients with abduction and adduction disabilities, Western treatment for those with flexion disability, and collab-orative approach for those with extension disability.

상아질 형성 부전증에 대한 증례보고

김희진,위유민,박성규,홍성수,조태식,이상호 조선대학교 구강생물학연구소 2002 口腔生物學硏究 Vol.26 No.2

Dentinogenesis imperfecta is an inherited disorder of dentin formation, usually exhibiting an autosomal dominant mode of transmission. Type Ⅰdentinogenesis imperfects occurs in Patients afflicted with osteogenesis imperfecta. Type Ⅱdentinogenesis imperfecta is not associated with osteogenesis imperfects. Type Ⅲ dentinogenesis imperfecta (Brandywine type) occurs in a racial isolate area in the state of Maryland. Clinically, the color of teeth may range from a gray to brownish violet or yellowish brown with translucent or opalescent hue. The enamel often chips and fractures away, allowing speedy attrition of the remaining, poorly calcified. softer dentin. Roentgenographic features include pulpal obliteration, bulbous crowns and short and blunted roots. The cementum, periodontal membrane and the supporting bone appear normal