임상 증상이 심하지 않은 심낭 삼출 환자의 장기 추적 결과

배장호,김기식,강미숙,남명희,김미정,김민수,김기영,님창욱,김윤년,김권배,정시전,김여희 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.7

Function of the pentose phosphate pathway and its key enzyme, transketolase, in the regulation of the meiotic cell cycle in oocytes

Kim, Yunna,Kim, Eun-Young,Seo, You-Mi,Yoon, Tae Ki,Lee, Woo-Sik,Lee, Kyung-Ah The Korean Society for Reproductive Medicine 2012 Clinical and Experimental Reproductive Medicine Vol.39 No.2

Objective: Previously, we identified that transketolase (Tkt), an important enzyme in the pentose phosphate pathway, is highly expressed at 2 hours of spontaneous maturation in oocytes. Therefore, this study was performed to determine the function of Tkt in meiotic cell cycle regulation, especially at the point of germinal vesicle breakdown (GVBD). Methods: We evaluated the loss-of-function of Tkt by microinjecting Tkt double-stranded RNAs (dsRNAs) into germinal vesicle-stage oocytes, and the oocytes were cultured in vitro to evaluate phenotypic changes during oocyte maturation. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression of other enzymes in the pentose phosphate pathway were determined after Tkt RNA interference (RNAi). Results: Despite the complete and specific knockdown of Tkt expression, GVBD occurred and meiosis was arrested at the metaphase I (MI) stage. The arrested oocytes exhibited spindle loss, chromosomal aggregation, and declined maturation promoting factor and mitogen-activated protein kinase activities. The modified expression of two enzymes in the pentose phosphate pathway, Prps1 and Rbks, after Tkt RNAi and decreased maturation rates were amended when ribose-5-phosphate was supplemented in the culture medium, suggesting that the Tkt and pentose phosphate pathway are important for the maturation process. Conclusion: We concluded that Tkt and its associated pentose phosphate pathway play an important role in the MI-MII transition of the oocytes' meiotic cell cycle, but not in the process of GVBD.

비만 여성을 대상으로 한 해조류 추출물의 국부처치와 운동 병행을 통한 지방분해 효과의 임상적 검정

서태수,이인숙,변재철,김규호,박승한,김유영,김상국,김현정,김상기,신동철,성미영,박종석,김종기,박언휘 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3

This study was carried out to test the body fat breakdown during topical seaweed extract treatment and in combination with the aerobic exercise. Of 20 female obesity subjects, ten treated a cream types of seaweed extract(control group) and ten treated a placebo cream(placebo group). Topical treatment of each sample was designed for 4 weeks, and treated with 2 times a day about 10ml on the abdomen and the thigh, respectively. All subjects were participated aerobic dance half hour a day and four times a week. After 4 weeks of topical treatment and exercise, they were measured body fat, skinfold thickness, body circumference. Then venous blood samples were taken and analyzed blood cells, serum enzymes, and serum lipids. There were reduction of body weight, % of body fat, antebrachium, brachium, chest, waist, thigh circumference and abdomen thickness between pre and post treatment in both group. Especially, body weight and % of body fat significantly reduced in the subjects treating the seaweed extract than those treating the placebo. There were significant decrement in total cholesterol and LDL whereas increment in HDL and TBIL in the control group, but no significant difference in the placebo group. In conclusion, there were 7.6% of body fat reduction during 4 weeks of aerobic exercise and 21% of body fat reducement in combination of topical seaweed treatment and exercise, respectively. These results suggest that the topical treatment of seaweed extract should be very effective for fat breakdown. Especially, the body fat breakdown maximized in the combination with the topical seaweed extracts treatment and the aerobic exercise.

암 병동 간호사의 통증관리에 대한 지식과 태도

공성화,방찌야,서민숙,현신숙,김희정,이미애1,이미애2,유현희,허재경,김은애,박경숙 성인간호학회 2004 성인간호학회지 Vol.16 No.1

Purpose: The purpose of this study is to evaluate the knowledge and attitudes of oncology ward nurses toward cancer pain management and to find ways to improve the educational program for nurses. Method: A total of 209 nurses working at the oncology ward of three hospitals in Seoul and a Gyenggi Province. The survey instrument used was the 32-item scale for evaluating nurses knowledge and attitudes originally developed by McCaffery and Ferrell'(1990). that was by Kim'(1997). Result: In terms of the nurses knowledge of pain management. the result showed that the nurses scored an average of 67.8 out of 100 for phamacokinetics of opioids. 84.8 for classification of analgesics, 60.1 for pain assessment, and 70.7 for drug administration. 18.2% of the nurses hesitate to inject the narcotic agent because of concerns regarding the drug's potential side effects. there was significant difference in the knowledge of pain management according to the general characteristics of pain in terms of the nurses age(p=.001), position (p=.016). years of experiences(p=.002), experience of cancer pain education(p= .001). Conclusion: The also showed that nurses working at cancer ward lack knowledge. It is important to provide intensive education to nurses about cancer pain management.

간호사의 이직의도 구조모형

김창희,양승순,김연자,손연정,유미애,송주은 대한간호학회 간호행정학회 2009 간호행정학회지 Vol.15 No.4

Purpose: The purpose of this study was to construct and test a structural equation model of nurses' turnover intention. Method: The participants were 716 nurses at 4 university hospitals. Data were collected by a self-report questionnaire in 2008 and analyzed using SPSS 12.0 and AMOS 4.0 Win program. Results: The fit indices of modified model were x²=285.928(p<.001),x²/df=1.958GFI=0.964, RMSEA=0.037, AGFI=0.944, NFI=0.947, NNFI=0.961, CFI=0.973, PNFI=0.658 and showed high compatibility with the empirical data. In the final model, job stress and satisfaction were found to have significant direct effects on nurses' turnover intention, while organizational culture and emotional labor were very important factors to have indirect effects on turnover intention via job stress and satisfaction. Nurses' turnover intention was accounted for 41.2% of covariance by these factors. Conclusions: Comprehensive nursing interventions for decreasing nurses' turnover intention should be focused on reducing job stress and increasing job satisfaction. Also, Nursing strategy for enhancing positive organizational culture and decreasing emotional labor would be helpful for decreasing nurses' turnover intention, too. Further studies are needed to prospectively conduct to verify these causal relationships with larger sample.

靑少年 勤勞者의 營養攝取 實態에 관한 調査硏究

김성미,차유미 啓明大學校 生活科學硏究所 1991 科學論集 Vol.17 No.-

The purpose of this research is to clarify the relationship between the situation of the industrial worker's nutritive conditions and elements which have influence on it. To satisfy this purpose, subjects of the investigation were the industrial laborers of eleven of many business enterprises in Young nam district, and the researching method questionnaire and field investigation. On the basis of these facts analysis and result of the general conditions, discomfortable physical symptoms, eating habits and nutritive conditions, etc. are summarized as follows. 1. Age of the male subjects was 26-29(47.7%) and that of the female subjects 20-25(94.2%). For the educational level male high school graduates were 83.6% and female high school graduates 60.6%. For the residential form 49.3% of male and 63.5% of female lived in the dormitary. the average monthly income of male was 310 thousand 400 thousand won(49.3%) and that of female 210 thousand 300 thousand won(61.3%). Monthly eating expenses of both male and female were 70 thousand won. For the discomfortable physical symptoms "fatuigable" was more than 60% in both male and female. And for the mental trouble "nervous about trifles" was 31.3%(male) and 56.2%(female) : percentage, of female was as two times as male. For meals skipping rate was 74.6% and female 86.8% : both of them showed the frequent meals skipping, and the time of meals skipping was mostly morning(male:82.0%, female:67.2%) Mixed and powdered eating rate was 70% in both sexes. 2. For the daily nutrition of subjects per capita calorte of male was 89% and that of female 65% of the Recommended Dietary Allowances for Koreans : and both were short of the Recommended Dietary Allowances for Koreans. For protein rate male was 114% (78g) and female 103% (62g) of the Recommended Dietary Allowances for Koreans: both of them were relatively good. And for ingestion of mineral nutirition and vitamine both sexes showed the good rate expect calcuim : which was over the Recommended Dietary Allowances for Koreans. 3. In the relation between general facts, eating habits and quantity of nutrition ingestion, the group which payed high eating expenses represented also high rate of nutrition ingestion except vitamin C. In residential form both sexes who lived in the dormitary showed high rate of nutrition ingestion. The lower rate of meals skipping was, the more nutrition ingestion quantity was. Between-meals refreshments were 1-2 times in both sexes, contests of those extended to all nutrients except vitamin C and vitamin B₂ The longer and more adequante rest was taken, the more nutrients were intaken, and in calcuim and Iron the significant difference (p<0.05) was indicated. It is true that the result of this research proves the higher rate in quantiry of nutrition ingestion compaved with other's But it is noticible that conditions of nutrition ingestion are very different according to such eating habits as degree of meals skipping, frequence of between-meals refreshments and conditions of rest. Therefore it is required to perform the better nutritive education for confirming the desirable eating habits.

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.

급성 구강감염부위에서 Staphylococcus lugunensis 의 특성

유용욱,이미성,차정단,김기경,신상희,문상은,김강주 원광대학교 생명공학연구소 1999 생명공학연구소보 Vol.6 No.1

Staphylococcus lugdunensis (S. lugdunensis) is a newly identified pathogenic species of coagulase negative staphylococci and an occasional but not rare cause of severe infections, such as infective endocarditis after dental extraction, bacteremia, osteomyelitis, peritonitis, and soft tissue infections. As antibiotic use increased, resistance rapidly developed. Some strains have plasmids related to antibiotic resistance. To characterize S. lugdunensis in acute oral infection, S. lugdunensis was isolated from the patients with acute oral abscess, osteomyelitis, and normal persons. Antibiotic resistance, in vitro cellular toxicity, in vivo virulence, δ-like hemolysin activity, and synergistic hemolysis on sheep blood agar plates were investigated. The dot blot analysis and Southern blot analysis of staphylococcal DNA was performed with δ-hemolysin gene probe of Staphylococcus aureus. (S. aureus) Staphylococcal DNA was cloned, nucleotide sequence was analysed, and homology was compared with other sequence in Gene Bank. S. aureus, S. lugdunensis, Staphylococcus cohnii (S. cohnii), and other coagulase negative staphylococci (CNS) were isolated from the patients with acute oral infection. The isolation ratio of S. lugdunensis in the patients with infection was higher than that of healthy persons, but the isolation ratio of S. aureus in the patients with infection was similar to that of healthy persons. S. lugdunensis from the patients with acute oral infection showed the resistance to penicillin, methicillin, cephalothin, and clindamycin. S. lugdunensis in the patients had cellular toxicity in vitro and virulence in vivo. All strains of S. lugdunensis had δ-like hemolysin activity against rabbit erythrocytes. Four of the six strains of S. lugdunensis gave synergistic hemolysis with S. aureus on sheep blood agar plates. In dot blot analysis, all strains of S. lugdunensis showed the positive reaction with the probe of δ-hemolysin gene in S. aureus, but a 7.3 kb HindⅢ fragment was observed in the DNA of S. lugdunensis that gave synergistic hemolysis in a Southern blot analysis. The molecular size of partially purified δ-hemolysin was about 50 kd. The cloned fragments from the chromosomal DNA of S. lugdunensis showed the partial homology with the insulin receptor-related and dopamine receptor of humans. These results suggest that S. lugdunensis might be an important pathogen in acute oral infection and show some homology with eukaryotes.

소목(Caesalpinia sappan) 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성 억제에 미치는 영향

유용욱,유현희,김윤정,유미선,서세정,이황,이흥수 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

Streptococcus mutans is known as the causative bacterial playing the most important role in forming plaque and it is being noticed as major causative bacteria of dental caries. Therefore, development of more effective, substantial and safe preventive agent against dental caries and periodontal disease is honestly required. The present study was designed to investigate the effect of chloroform extract(CE), n-butanol extract(BE),methanol extract(ME) and water extract(WE) of Caesalpinia on the growth, acid production, adhesion, and insoluble glucan synthesis of Streptococcus mutans. The CE, BE, ME and WE decreased the growth and acid production of S.mutans than that of control, especially, the ME was more effective than other extracts. The effect of CE, BE, ME and WE on the adherences to saliva-coated hydroxyapatite bead(S-HA) were evaluated. At the concentration of 2,000 ㎍/㎖, the CE showed 32% rate of bacterial adherence to S-HA, whereas, other extracts of the same concentration producedgreater than 50% rate. The CE and BE were significantly decreased the reduction rates of water insoluble glucan formation from sucrose by crude glucosyltransferase of S.mutans. Thus, the application of C. sappan can be considered a useful and a practical method for the prevention of dental caries.

특수학교의 재구조화 방안

김주영,김병하,이미선,이유훈 국립특수교육원 2000 연구보고서 Vol.- No.2

통합교육은 특수학교 중심 의 전통적 인 분리교육에 대응한 개념으로 이해 할 수 있다. 1994년 특수교육진흥법이 전면 개정되면서 우리나자는 장애학생 교육의 전달체계를 통합교육을 촉진하는방향으로 개 혁해야 한다는 수장이 대두되 기 시작하였다. 특수교육 전달체 계 의 개 혁은 곧 분리교육의 해체를 뜻하는 것이라고 여겼기 때문이다. 이와 같은 관점에서 현재 특수교육 전달체계 가운데가장 큰 개혁의 초점은 바로 특수학교가 될 것이다. 왜냐하면, 그동안 특수학교는 분리교육의 중심으로 여겨져 왔을 뿐만 아니라, 가정이나 병원, 시설에서 이루어지는 장애학생의 교육도 결국은그 거점이 특수학교가 될 가늘성이 높기 때문이다. 그러므로 특수교육이 기존의 분리교육 체제를해체하고 통합교육의 체제로 옮아가기 위해서는 필연적으로 특수학교의 역할과 기능을 제고함으로써 통합교육 체제에 순응하고 적극 기 여 할 수 있는 전달체 계로서 의 새로운 특수학교 구조를 제시할 필요가 있는 것이다. 그것은 바로 바로 특수학교의 재구조화를 의미하는 것이다. 이에 따라,본고에서 제시하고 있는 우리나라 특수학교의 재구조화 방향과 그에 따른 방안은 다음파 같다.특수학교 재구조화 방향한국적인 상황에서 급진적 통합교육의 추진은 자칫 장애학생의 행복을 저해하고 장애의 특성과정도에 따른 다양한 교육서비스의 선택 폭을 줄일 뿐만 아니라, 기존의 엄청난 특수교육 자원을낭비할 수도 있다는 것이 현재 특수교육계의 지배적인 생각이다. 따라서 특수학교는 앞으로 중증 · 중복장애 학생의 교육 기 관화와 지 역사회 중심 의 특수교육 센터화, 특수교육의 특성화와 전문화의 길로 나아가는 것이 바람직할 것으로 기대된다.특수학교 재구조화 방안 I : 특수학교의 운영 체제 전환첫째, 특수학교는 장애의 진단 평가 및 상담과 중중 ·중복장애차생 교육, 재택 순회교육, 일반학교의 특수교육 지원, 지역사회 장애이해 증진 등에 걸쳐 그 학교의 역할을 제고해야 한다둘째,특수학교는 일반학교 내에 특수학교를 두는 형태, 특수학교에 일반학생들을 받아들이는형태, 장애영역을 통합하는 형태, 작은 규모의 형태, 경도장애학생들을 위한 실업 고등학교 등 학교의 형태를 다양화해야 한다. 셋째,특수학교는 교육과정을 생활기능 중심,통합교과 중심의 총체적 교수방법 적용,개별화교육계획(IEP)의 탄력적 적용 등을 제고해야 한다.넷째, 특수학교는 교원의 특수교육-일반교육 근무체제를 활성화하고, 특수교육 담당 교사에 대해 우대하며, 우수인력의 고른 배치와 현직교원의 교차 연수기회를 부여함으로써 교원의 자질과전문성을 신장해 야 한다.다섯째, 특수학교는 지역내 순회교육 대상 장애학생들의 교육을 중점적으로 담당해야 한다.석섯째,특수학교는 학생 중심,지역사회 적응 중심의 전환교육을 바탕으로 한 직업교육을 체계적으로 실시해야 한다.일곱째,특수학교는 부모의 의견을 수렴하여 학교 교육과정 운영에 적절히 반영하는 한편,자녀의 발달단계에 따른 발달과업을 중심으로 부모교육을 실시해야 한다.특수학교 재구조화 방안 ll : 국가 및 지방자치단체의 변화국가 및 지방자치단체는 새로운 조직이나 사업 개발에 착수하기보다는 기존의 조직과 사업을활용하면서 교원 각자의 참여를 유도할 수 있는 통합교육 정책 개발과 투자에 힘을 쏟아야 하며,지방 특수교육의 안정적 발전을 위해 지속적으로 예산 지원을 보장해야 한다. 또한 예산의 안정적지원과 함께 국가는 지방특수교육에 대한 평가를 정례화 하여 그 성과에 따라 특별교부금을 지급하거나 적정 투자권고 등 행정지도를 강화해야 한다.특수학교 재구조화 방안 In : 지역사회 중심의 특수학교 체제첫째, 특수학교는 학교간 교류협력의 활성화와 지역사회 자원을 활용한 경험 중심의 교윽과정의 실천,지역사회 자원을 활용한 직업교육의 실시,자원봉사 인력의 활용 등을 통한 지역사회 자원을 활용한다.둘째, 특수학교는 학교 시설의 제공, 각종 문화행사, 장애인 및 그 가족을 지원함으로써 지역사회 센터로서 기여해야 한다.지금까지 제시한 특수학교 재구조화의 방향과그에 따른 방안들은 우리나라의 전통적 특수교육상황을 적극적으로 개선하기 위해 반영한 제 안들이 다. 그러나 이 러 한 방안을 제시하는 것만으로특수학교의 재구조화가 실현되는 것은 아니다. 국가는 국가수준에서, 학교는 학교수준에서 이미제시된 방향을 향해 구체적인 실천과제를 정하여 하나 하나 구체적으로 실천해 갈 때 비로소 가능해 지는 것임을 잊어서는 안될 것이다.