Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

고압 커패시터의 고장 분석을 통한 신뢰도 예측

양석준,김진우,신승우,이희진,신승훈,유동수,장석원 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.6

본 논문은 고압 커패시터의 고장분석과 신뢰성 예측 결과를 다루고 있다. 부품의 수명과 고장률을 예측하기 위해서 두 가지 방법으로 고장 모드와 고장 메커니즘을 연구하였다. 에폭시 수지로 성형된 고압 커패시터가 절연내압 시험 하에서 저항이 제로로 되는 고장에 대하여, 근본원인 고장분석 체계를 효과적으로 수립함으로써 고장 메커니즘의 원인을 분석하였다. 특히 세라믹-에폭시 계면에서의 절연파괴 고장 현상이 강조되었으며, 본 연구에서 얻어진 결과의 타당성은 마그네트론에 장착된 고압 커패시터의 열사이클 시험 수행에 의한 가속시험 결과로부터 입증되었다. 시험 결과들은 결함이 있는 로트를 신속히 규명하고 B10수명을 결정하는데 유용하게 사용할 수 있다. 또한, 유전체의 절연파괴에 대해서 부하-강도 간섭모델을 이용하여 고장률을 예측하였다. This paper presents a result of failure analysis and reliability evaluation for high voltage ceramic capacitors. The failure modes and failure mechanisms were studied in two ways in order to estimate component life and failure rate. The causes of failure mechanisms for zero resistance phenomena under withstanding voltage test in high voltage ceramic capacitors molded by epoxy resin were studied y establishing an effective root cause failure analysis. Particular emphasis was placed on breakdown phenomena at the ceramic-epoxy interface. The validity of the results in this study was confirmed by the results of accelerated testing. Thermal cycling test for high voltage ceramic capacitor mounted on a magnetron were implemented. Delamination between ceramic and epoxy, which might cause electrical short in underlying circuitry, can occur during curing or thermal cycle. The results can be conveniently used to quickly identify defective lots, determine B10 life estimation each lot at the level of inspection, and detect major changes in the vendors processes. Also, thd condition for dielectric breakdown was investigated for the estimation of failure rate with load-strength interference model.

프로스타글란딘 E₁에칠에스테르의 외용 리오겔 제제 설계

양성운,이진교,이지은,김희규,박혜숙,김종석,최한곤,용철순,최영욱 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

External lyogels containing prostaglandin E₁ethyl ester(PGE₁-EE), a produrg of prostaglandin E₁(PGE₁) as a therapeutic agent for erectile dysfunction, were formulated to overcome the aqueous instability and enhance the percutaneous absorption. Lyogels of PGE₁-EE were prepared with ethanol (EtOH)/proplyene glycol (PG) cosolvent system as a vehicle, cineol as an enhancer, and hydroxypropylcellusose as a gelling agent. In vitro percutaneous absorption studies were performed to determine the rate of PGE₁ absorption through rat or hairless mouse skin. The permeability of PGE₁-EE lyogel with enhancer was 16-fold greater than that of lyogel without enhancer. Cosolvent produced 9-fold increase in percutaneous absorption. Pharmacodynamic effects of lyogels were evaluated in mature male cats in terms of intracavernosal pressure (ICP). Lyogles containing 0.01% of PGE₁-EE showed higher ICP compared to intraurethral preparation of PGE₁(1%) and enhancer-free control lyogel. The shelf-life (t_(10%) of lyogel at refrigerated condition (4℃) was calculated as 928 days, which is 4.2 times longer than that of control hydrogel. As a result, PGE₁-EE was formulated successfully to a lyogel system with a selective enhancer and cosolvent system for the topical delivery of PGE₁.

쾌적한 캠퍼스 조성을 위한 기본계획 기초적 연구

梁富弘,朴碩鉉,崔生吉,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

In this study, the potential energy and limitation of the facility of Chungju national university are analyzed in the respect of human knowledge, nature, and visual environment. In addition, the effctiveness and problem of the existing master plan are evaluated through the investigation on the main hart and development procedure of the previous master plan of CJU. These results are compared and reflected to the plan of Vision 2003, e-STRR2010 and effective campus development plans of CJU are presented.

不正手票團束法에 관한 小考

梁慧振,宋錫彦 濟州大學校 法과政策硏究所 1998 法과 政策 Vol.- No.4

거대 긴장성 낭종으로 내원한 폐 Langerhans 세포 조직주증

양석철,손장원,윤호주,신동호,박성수,함시영,장세진,박용욱 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.1

Pulmonary Langerhans cell histiocytosis is a hetergenous group of conditions of unknown etiology characterized by an abnormal proliferation of antigen presenting cell of bone marrow derivation known as Langerhans cells that invade and destroy distal bronchioles. The outcome is highly variable, ranging from rapid spontaneous resolution to irreversible respiratory failure. We describe a patient with biopsy-confirmed pulmonary Langerhans cell histiocytosis who presented with large tension cyst, treated with pneumothorax tube with Heimlich flutter valve.

FEM에 의한 차량전면부 사이드부재의 축방향 충격압궤 해석

차천석,정진오,양인영 한국안전학회 2003 한국안전학회지 Vol.18 No.4

The front-end side members of vehicles(spot welded hat and double hat shaped section members) absorb most of the impact energy in a case of front-end collision. In this paper, specimens with various spot weld pitches have been tested with a high impact velocity of 7.19m/sec(impact energy of 1034J). The axial impact collapse simulation on the sections has been carried out to review the collapse characteristics of these sections, using an explicit finite element code, LS-DYNA3D. Comparing the results with experiments, the simulation has been verified; the energy absorbing capacity is analyzed and an analysis method is suggested to obtain exact collapse loads and deformation collapse modes.

흉골골절이 의심되는 외상환자에서 초음파의 진단적 효용성

염석란,김재광,이근,박철완,양혁준,현성열,임용수,진욱 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Purpose: This study compared the value of ultrasonography(US) in the diagnosis of sternal fractures with those of conventional radiography and bone scan. Material and Method: From March 2000 to March 2001, in Gil Medical Center, Gachon Medical College, 44 patients(22 women and 22 men, mean age 46 years, range 5∼81 years) complained of tenderness in the sternal area. The cases histories of these patients with clinically suspected sternal fracture due to blunt trauma were prospectively studied. At the time of admission, conventional radiography(sternal view) was done. At one day after admission, sternal ultrasonography was obtained, and after 4∼5 days, a bone scan was done. Sternal ultrasonographic findings, conventional radiographic findings, and bone-scan findings were compared. In addition, a 12-lead ECG was done. When it was necessary, cardiac enzyme was checked and echocardiography was performed. Result: In 21 of 44 patients(48%), a sternal fracture was found by sternal US. Only 9 of 21 patients (43%) were diagnosed as having a sternal fracture by using conventional radiography. 23 of 44 patients (52%) have negative findings on sternal US; they also had negative findings on conventional radiography. Except for 7 unchecked patients, 13 of 21 patients(62%) were diagnosed as having a sternal fracture by bone scan, and among the 23 patients who had negative findings on sternal US, the 19 patients checked with a bone scan also had negative findings. Conclusion: Sternal US can find sternal fractures that are difficult to find on conventional radiography. Compared with bone scan, sternal US takes a shorter time and sternal US is not difficult. Additionally, combined lesions with sternal fractures(peristernal hematoma, soft tissue swelling) and costar cartilage fractures are diagnosed more easily when using sternal US.

Terfenadine과 Ketoconazole 병용투여에 의해 유발된 Torsade de Pointes1예

오석규,국향,임수빈,정진원,박양규,박옥규 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.3

핸드스프링 몸펴 앞공중1회 비틀기 동작의 소요시간 및 각운동량 분석

권오석,윤양진,서국웅 한국운동역학회 2002 한국운동역학회지 Vol.12 No.2

남자 기계체조 국가대표 선수 3명과 대학선수 3명을 대상으로 핸드스프링 몸펴 앞공중1회 비틀기 동작을 구간 및 국면별로 분석하고 동작의 숙련도에 따라 역학적 변인이 집단 간 어떠한 차이를 보이는지를 구명하기 위하여 Kwon3D 프로그램을 활용하여 분석한 결과 다음과 같은 결론을 얻었다. 시간요인에서 보폭을 크게 딛는 것이 손을 빠르게 지지 할 수 있고, 핸드스프링 체공시간은 짧게 하여야 발구르기의 압력을 높여 비틀기동작에서 체공 소요 시간이 길어지므로 비틀기 수행의 완성도를 높일 수 있는 것으로 나타났다. 각운동량 요인에서 기술수행 시 전체적으로 좌우(X)축에 대한 각운동량이 전후(Y)축과 수직(Z)축에 대한 각운동량에 비해 더 큰 값을 보였다. 좌우 축 각운동량은 지면에서 이지되어 동작을 수행하는 핸드스프링회전과 앞공중1회 비틀기에서 숙련자가 상대적으로 더 큰 각운동량 보이는 것으로 나타났다. The purpose of this study were a two-fold: ⑴ to analyze event and phase of handspring salto forward stretched with turn; ⑵ to know the differences in the kinematic variables between two groups. A Kwon3D program served for the estimation of this study. The group was divided into three National representative and three well-trained calisthenics in this study. The results of this study revealed that ⑴ the forward somersault performance was increased when duration time in the air was long during the salto forward stretched that the duration time of Handspring is short, the pressure at takeoff is high, the stride is large, and hands are supporting on the ground quickly; ⑵ comparing the angular movement of anterior and posterior y axis and vertical z axis, the angular movement of right and left x axis was higher during the performance. As a result, the national representative players showed better performance in Handspring salto forward stretched with turn.