3차원 콘빔CT 영상에서 형성한 2차원 정모두부방사선사진영상의 정확도 증진을 위한 두부자세재현기의 효과

선민규,엄기수,조진형,황현식 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.5

Objective: The purpose of the present study was to evaluate the effectiveness of the use of Head Posture Aligner (HPA) during cone-beam computed tomography (CBCT) scan in generation of frontal cephalograms using 3D CBCT images. Methods: CBCT scans and frontal cephalograms were made in 30 adult individuals. While a couple of CBCT scan was made for one subject, one was made with conventional method, without use of HPA, the other was acquired with the use of HPA. After creation of virtual frontal cephalogram from each 3D CBCT image, it was traced and compared with the tracing of real frontal cephalogram. Results: In the comparison of the measurements, the virtual cephalograms with the use of HPA did not show statistically significant differences with the real cephalograms whereas the virtual cephalograms without the use of HPA presented significant differences with real cephalograms in many measurements. In the correlation analysis with the measurements of the real cephalograms, the virtual cephalograms with the use of HPA showed higher correlations in all measurements than the virtual cephalograms without the use of HPA. Conclusions: Measurements from CBCT-generated cephalograms become similar to those from real cephalograms with the use of HPA during CBCT scan. Thus, the use of HPA is suggested during the CBCT scan in order to construct accurate virtual frontal cephalograms using 3D CBCT images. 본 연구는 3차원 콘빔CT 영상을 이용하여 2차원 정모두부방사선사진영상 형성 시 두부자세재현기(Head Posture Aligner, HPA)를 이용한 경우와 이용하지 않은 경우를 비교분석함으로써 HPA의 유용성을 평가하기 위하여 시행되었다. 성인 30명을 대상으로 먼저 일반적인 방법으로 콘빔CT 영상을 채득한 후 HPA를 이용하여 일정한 두부자세를 재현한 상태에서 또 하나의 콘빔CT 영상 및 정모두부방사선사진을 촬영하였다. 3차원 영상프로그램을 이용하여 HPA를 이용하지 않은 경우와 HPA를 이용한 경우 각각에서 2차원 정모두부방사선사진영상을 형성한 다음 각각의 투사도를 작성하고 실제로 HPA를 이용하여 촬영된 정모두부방사선사진 투사도와의 차이를 비교하였다. 거리 및 각도를 나타내는 여러 계측치를 비교분석한 결과 HPA를 이용하지 않은 경우에는 다수의 계측항목에서 실제 정모두부방사선사진과 유의한 차이를 보인 반면 HPA를 이용한 경우에는 모든 항목에서 통계적 유의차를 보이지 않았다. 아울러 실제 정모두부방사선사진과의 상관관계를 알아보기 위해 상관분석을 시행한 결과 HPA를 이용한 경우가 그렇지 않은 경우보다 모든 항목에서 높은 상관성을 보였다. 본 연구 결과는 3차원 콘빔CT 영상을 이용하여 2차원 정모두부방사선사진영상 형성 시 정확한 영상 획득을 위해서는 콘빔CT 촬영 시 HPA의 사용이 필요함을 시사하였다.

강원도산 참당귀와 일본산 일당귀의 생리 활성 성분 탐색

함문선,김성수,홍종수,이진하,정을권,박영식,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

참당귀와 일당귀를 에탄올로 추출하였고 이들 추출물들을 디에틸 에테르층과 물층으로 분획하였다. 두 개의 분획물들은 Ames mutagenicity test에서 MNNG(0.5 ㎍/plate)에 대한 강한 항돌연변이 효과를 나타내었다. 디에틸 에테르 분획물들은 MNNG의 돌연변이원성을 78%에서 80%까지 억제시켜 가장 강한 돌연변이 유발 억제효과를 보여주었다. 각각의 에탄올 추출물(1㎍/㎕)들은 사람의 다양한 암세포들의 성장을 억제시켰다. 특히, 디에틸 에테르 분획물들은 간암 세포의 생육을 90%에서 95%까지 억제시켜 가장 강한 항암효과를 보여주었다. 그러나 수층 분획물들은 암세포의 생육에 대하여 거의 억제활성을 나타내지 않았다. 에탄올 추출물들은 인체의 정상 간세포에 대하여 거의 세포독성을 나타내지 않았다. 참당귀의 디에틸 에테르 분획물들은 일당귀와 비교하여 20% 미만의 세포독성을 보여주었다. 참당귀와 일당귀 디에틸 에테르 분획물들의 0.5㎍/㎕ 첨가는 T세포와 B세포의 생육의 3~4배 정도 증가에 의한 면역활성을 보여주었다. 또한 참당귀의 디에틸 에테르 분획물(0.7㎍/㎕)이 ACE 활성을 98%까지 억제시켜 혈압을 조절할 수 있음도 증명되었다. TLC로부터, 두 개의 디에틸 에테르 분획물들은 참당귀와 일당귀의 활성 성분중의 하나로 알려진 umbelliferon을 함유함을 알 수 있었다. The ethanol extracts from Anglica gigas Nakai and Angelica acutiloba Kitagawa were fractionated to diethyl ether and aqueous partitions. Both partitions had strong antimutagenic effect on the MNNG (N-methyl-N-nitro-N-nitrosoguanidine) by Ames mutagenicity of MNNG with inhibition of 78~80%. The ethanol extracts from both species showed the inhibitory effect on the growth of several human cancer cell lines. Especially, the diethyl ether fraction from ethanol extracts was most effective on human hepatocellular carcinoma cells, inhibiting 90~95% of cell growth. However, the aqueous fractions had least inhibition activity on many cancer cells. There was little cytotoxicity on human normal liver cell by ethanol extracts. Diethyl ether fraction from Angelica gigas Nakai ethanol extract had cytotoxicity les than 20% on human normal liver cells, compared with that from Angelica acutiloba Kitagawa ethanol exract. The adding of 0.5 (g/ℓ) of diethyl ether fractions of Angelica gigas Nakai or Angelica acutiloba Kitagawa increased immune activity by enhacing human B and T cells up to three to four times. It was proven that diethyl ether fraction (0.7 g/ℓ) from Angelica gigas Nakai could control blood pressure by suppressing angiotensin converting enzyme activity up to 98%. From TLC, it was appeared that both of diethyl ether partitions had umbelliferon, known to one of active substances from Angelica gigas Nakai and Angelica acutiloba Kitagawa.

노인외상환자의 외상중증도계수와 동통감지력의 변화에 대한 임상적 고찰

이진웅,임경수,김영식,황성오,이강현,김선만,김 현 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Because elderly individuals are participitating in social work as adult inspite of their physical handicapes such as hearing difficulty, visual difficulty and limit of motion, they are continually exposed to the risk of injury. Trauma is now fifth cause of death in elderly of over 65 years old, and the elderly suffer disproportionately high injury-related motality comparing with younger adults. In addition, sometimes elderly patients don`t complaint of pain on injured site because of high threshold to pain. We studied about occurence of neglected injury due to high threshold to pain in geriatric trauma patients. When we diagnosised injury that patients did not complaint of pain on injured site through physical examination and radiologic examination, we defined that as neglected injury. There were 8 (8%) geriatric patients who didn`t complaint of pain on injured site on admission to emergency center, but only 0.7% of adult patients had neglected injury. The severity was estimated by use of AIS(Abbreviated Injury Scale), and the severity of all neglected injuries were above AIS 2 point. The neglected injury were head injuries(4 in number), abdominal injury(1), pelvic injuries(2) and extremity injury(1). So if geriatric trauma patients were admitted to emergency center, we recommand full physical and radiological examinations although patients don`t complaint of pain.

발화기구의 정량적 해석을 위한 재현 실험 : 1차년도 전기화재 재현 Reconstruction for Electrical Fire

박남규,김상현,김진표,김윤희,문병선,조영진 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

파노라마방사선사진 지수와 임플란트 실패와의 관계에 관한 연구

조현정,이원진,허민석,안창현,이진구,이삼선,최순철 大韓口腔顎顔面 放射線學會 2004 Imaging Science in Dentistry Vol.34 No.1

Purpose : Several panoramic indices have been suggested to assess bone quality from the morphology and width of mandibular cortex on panoramic radiography. The purpose of this study was to compare dental implant failure group with control group in panoramic mandibular index (PMI), mandibular cortical index (MCI), and gonion index (GI) and to determine the effect of these panoramic indices on dental implant failure. Materials and Methods : A case-control study was designed. Test group (n = 42) consisted of the patients who had their implants extracted because of peri-implantitis. Control group (n = 139) consisted of the patients who retained their implants over one year without any pathologic changes and had been followed up periodically. They had dental implants installed in their mandibles without bone augmentation surgery from 1991 to 2001. The following measures were collected for each patients: 1) PMI, MCI, and GI were measured twice at one-week interval on preoperative panoramic views; and 2) age, sex, implant length, implant type, installed location, occluding dentition state, and complication were investigated from the chart record. Results : The PMI showed moderate level of repeatability. The intra-observer agreement of MCI and GI were good. There was statistically significant difference in PMI between two groups. There were significant different patterns of distribution of MCI and GI between two groups. Among the panoramic indices, PMI and MCI showed significant correlation with dental implant failure. Conclusion : Panoramic indices can be used as reference data in estimating bone quality of edentulous patients who are to have implants installed in their mandibles.

KS H ISO 1242 표준의 평가 : 라반딘 정유의 산값 측정

전혜림,이은지,장혜진,손현화,김건,김남선,전명숙,이동선 서울여자대학교 2010 자연과학연구논문집 Vol.22 No.-

The acid value (AV) as well as the ester value of essential oil is an important index to evaluate quality of essential oil. The official standard analytical method of KS H ISO 1242 dealing with “Essential oils - Determination of acid value” was assessed experimentally using two lavandin essential oils obtained in France as an example. One French lavandin oil sample showed the results of AV= 0.9 by both indicator method and potentiometry. Another sample showed AV = 1.9 by indicator method and AV = 1.6 by potentiometry. Latter lavandin oil sample exhibited significantly higher acid value than maximum value of 1 described in the standard. This result suggests that latter lavandin oil was contaminated, because this oil was contained in an orange juice bottle. Results of this study proved that KS H ISO 1242 could be used as a suitable scientific standard for the determination of acid value of essential oils.

저혈청 배지에서 인간 전골수세포(HL-60)를 이용한 tPA 생산과 세포사멸기작에 관한 연구

김현구,성기돈,김태호,안주희,함문선,박진서,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

tPA 생산과 세포 사멸의 연구를 하기 위해서 인간 전골수세포가 배양되었다. 연속 관류 배양하에서 최대 tPA 생산량은 324 ng/ml이며 최대 세포 농도는 5.27×10 exp (6) cells/ml였다. Phorbol ester를 넣었을 때 tPA 생산량은 420 ng/ml까지 향상되었다. 형광 현미경으로 관찰했을 때, apoptosis는 세포 배양 초기부터 중반까지 우세했고 빠른 교반 속도에서도 우세했다. 그러나 necrosis는 세포배양 말기와 동결된 세포가 해동 되었을 때 우세했다. HL-60 was cultivated to produce tPA (tissue-type plasminogen activator) and study the mechanism of cell death. Maximum cell density and tPA production were obtained as 5.27×10 exp (6) cells/ml and 324 ng/ml, respectively under perfusion cultivation. tPA production was enhanced to 420 ng/ml in adding 160 nM of phorbol ester. The cells were gradually differentiated to granulocytes rather than proliferation. By Fluorescent microscope, apoptosis was prevailed except the death phase and in high agitation speed, but necrosis was prevailed in thawed cells and during the latter periods of the cultivation. It was also proved that tPA was most produced in apoptosis. To obtain higher tPA productivity, the cells must be maintained in apoptosis, not necrosis phase when the cells were dying.

Pfeiffer Syndrome Type 2 with Sporadic Fibroblast Growth Factor Receptor 2 Mutation and Coccygeal Anomaly

( Jin Sun Lee ),( Jin Hyuk Choi ),( Yong Wook Lee ),( Mi Hyeon Gang ),( Sun Kyoung You ),( Hyun Dea Shin ),( Mea-young Chang ) 대한주산의학회 2018 大韓周産醫學會雜誌 Vol.29 No.3

Pfeiffer syndrome is a rare genetic disorder characterized by premature fusion of certain skull bones (craniosynostosis) and anomalies of the face and extremities. A female newborn showing ocular proptosis and typical cloverleaf skull was considered as having Pfeiffer syndrome type 2. She also had coccygeal anomaly resembling a human tail. However, the accompanying vertebral malformations are rare in Pfeiffer syndrome. Molecular genetic testing confirmed sporadic fibroblast growth factor receptor 2 mutation in this patient. Thus, molecular genetic testing should be considered for any type of Pfeiffer syndrome to obtain definite diagnosis.

Production of Recombinant Miraculin Protein Using Transgenic Citrus Cell Suspension Culture System

Jin, Seong Beom,Sun, Hyeon Jin,Bachchu, Md Adnan Al,Chung, Sung Jin,Lee, Jongwoo,Han, Song-I,Yun, Jeong Hun,Boo, Kyung Whan,Lee, Dongsun,Riu, Key Zung,Kim, Jae-Hoon The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.3

Miraculin gene containing the N-terminal signal peptide was introduced into navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) callus cells by Agrobacterum-mediated transformation. Transgenic somatic embryos were screened on the shoot induction medium containing 25 mg hygromycin $L^{-1}$. Citrus callus cells were reproduced from the green color somatic embryos on the callus reproduction medium. The obtained transgenic cells were cultured in Murashige and Tucker's liquid medium containing 50 g sucrose $L^{-1}$ in a shaking incubator. Similar to the native miraculin, the secreted recombinant miraculin protein formed a disulfide-linked dimer and retained taste-modifying activity. The stability of recombinant protein expression was confirmed over nine generations of callus. This production system can be an excellent alternative for producing various recombinant proteins as well as miraculin.

Isolation and Identification of Anthocyanins contained Red Petals in Edible Rose

Hyeon-Jin Lee,Young-Sun Hwang,Su-San-Na Choi,Su-Jin Lee,Myoung-Gun Choung 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

The petals of edible rose contains diverse component, such as cratgolic acid, malic acid, vitamin C, B2, flavonoids, and lactons. The petals of edible rose are also known to have pharmaceutical effects. They have been used in the treatment of cardiac insufficiency, coronary heart disease, hyperpiesia and inflammatory disease only in folk remedies. Also, the petals of edible rose have various colors, such as red, pink, purple, blue, green, black, white, and yellow. Maybe, such color pigmentations are due to accumulation of anthocyanins, chlorophylls, flavonoids, and various combinations of breakdown products of these pigments. Despite many research on rose, report on the exact composition of anthocyanins in petal of edible rose is limited. Therefore, the objective of this study is to isolate and identify the anthocyanins in red petals of edible rose (Rosa hybrida cv. Noblered). Major anthocyanin pigments of the red petals in edible rose were extracted with 0.5% HCl - 20% MeOH for 24hrs. at room temperature, and purified using C18 open-column chromatography and prep-HPLC method. Two major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-VIS, LC-MS, and NMR). The λmax of anthocyanin 1 was 512nm, and m/z = 611[M+], while anthocyanin 2 had maximum absorbance at 501nm, and m/z = 595[M+], resulting in the two compounds are different. The complete structures of these anthocyanins were elucidated as cyanidin-3,5-diglucoside, and pelargonidin-3,5-diglucoside, respectively.