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( Xin Ran Li ),( Hui Xu ),( Jie Xie ),( Qiao Fu Yi ),( Wei Li ),( Dai Rong Qiao ),( Yi Cao ),( Yu Cao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the Vmax and specific activity of XynB reached 2,547.7 umol/mg and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at 80°C. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and e ventually i ncreasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.
Identification and Mapping of a Thermo-Sensitive Genic Self-Incompatibility Gene in Maize
Xin Ge Lin,Hui Ling Xie,Zhang Ying Xi,Yan Min Hu,Guang Yuan Zhao,Liu Jing Duan,Zong You Hao,Zong Hua Liu,Ji Hua Tang 한국유전학회 2009 Genes & Genomics Vol.31 No.3
In this study, we describe a novel ecological self-incompatibility (SI) line HE97 in maize. The main environmental factors influencing the inbred line characteristics were identified through field sowing trials during a two-year study period (2001 and 2002). The results showed that daily minimum temperature had the greatest effect on floral morphology and breeding system of the SI line. In staminate floret differentiation, when the daily minimum temperature exceeded 24℃, the line exhibited complete self-compatibility; however SI was observed when the daily minimum temperature was below 20℃. Therefore, we characterized the line as exhibiting thermo-sensitive genic self-incompatibility (TGSI). A set of F2 and F2:3 populations, derived from the inbred lines HE97 and Z58, were evaluated for two years to elucidate the TGSI line patterns of inheritance. Classical genetic analyses and QTL mapping results revealed that HE97 self-incompatibility was governed by a single allele, named here as tgsi1. The tgsi1 gene was mapped to chromosome 2 between SSR markers nc131 and bnlg1633, with a distance of 2.40 cM from nc131 and 2.44 cM from bnlg1633.
Guo Xin,Xie Yong-Xin,Guo Chen,Wei Jing-Lin,Yang Hui-Fang 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.2
Background In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent. Objective To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP). Results Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05). Conclusion The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats. Background In recent years, studies have shown that cyfluthrin is harmful to the reproductive system. Lycium barbarum polysaccharide may be a protective agent. Objective To investigate the effect of cyfluthrin (Cy) on testosterone B (inhibin B, INHB) in male rats and the intervention of Lycium barbarum polysaccharide (LBP). Results Compared with the control group, the mRNA and protein expression levels of INH α and INH βB in the testis tissue decreased, and the difference was statistically significant (P < 0.05). The LBP intervention group can significantly increase the expression level, and the difference is statistically significant (P < 0.05). Conclusion The decrease of INHα and INHβB expression caused by Cy exposure indicates that Cy has a toxic effect on the testis of rats, and the addition of LBP has a certain protective effect on the testis of rats.
Arjun Sinkemani,Xin Hong,Zeng-Xin Gao,Su-Yang Zhuang,Zan-Li Jiang,Shao-Dong Zhang,Jun-Ping Bao,Lei Zhu,Pei Zhang,Xin-Hui Xie,Feng Wang,Xiao-Tao Wu 대한척추외과학회 2015 Asian Spine Journal Vol.9 No.6
Study Design: Retrospective, case control evaluation of 86 patients who underwent microendoscopic discectomy (MED) and percutaneous transforaminal endoscopic discectomy (PTED) for the treatment of lumbar disc herniation (LDH). Purpose: To evaluate the safety and the outcomes of MED and PTED for the treatment of LDH. Overview of Literature: MED and PTED are minimally invasive surgical techniques for lower back pain. Studies to date have shown that MED and PTED are safe and effective treatment modalities for LDH. Methods: A retrospective study was performed in patients with LDH treated with MED (n=50) and transforaminal endoscopic discectomy (PTED; n=36) in our hospital. All patients were followed-up with self-evaluation questionnaires, Oswestry disability index (ODI), medical outcomes study 36-item short form health survey and MacNab criteria. All the patients in both groups were followed up to 12 months after the operation. Results: ODI questionnaire responses were not statistically different between the MED and PTED groups (53.00 vs. 48.72) before treatment. Average scores and minimal disability after 5 days to 12 months of follow-up were 4.96 in the MED group and 3.61 in the PTED group. According to MacNab criteria, 92.0% of the MED group and 94.4% of the PTED group had excellent or good results with no significant difference. Conclusions: There was no significant difference between MED and PTED outcomes. Further large-scale, randomized studies with long-term follow-up are needed.
Hu, Zheng-Hui,Lin, Yi-Wei,Xu, Xin,Chen, Hong,Mao, Ye-Qing,Wu, Jian,Zhu, Yi,Xu, Xiang-Lai,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Background: Many studies have investigated associations between the glutathione S-transferase M1 (GSTM1) null polymorphism and risk of prostate cancer, but the impact of GSTM1 in people who live in Asian countries is still unclear owing to inconsistencies across results. Methods: We searched the PubMed, Web of Science, Scopus, Ovid and CNKI databases for studies of associations between the GSTM1 null genotype and risk of prostate cancer in people who live in Asian countries, and estimated summary odds ratios (ORs) with 95% confidence intervals (95% CIs). Results: A total of 18 case-control studies with 2,172 cases and 3,258 controls were included in this meta-analysis, which showed the GSTM1 null genotype to be significantly associated with increased risk of prostate cancer in people who live in Asian countries (random-effects OR=1.74, 95% CI1.44-2.09, P<0.001). Similar results were found in East Asians (OR=1.41; 95% CI: 1.12-1.78; P=0.004) and Caucasians in Asia (OR=2.19; 95% CI: 1.85-2.60; P<0.001). No evidence of publication bias was observed. Conclusions: This meta-analysis of available data suggested that the GSTM1 null genotype does contribute to increased risk of prostate cancer in people who live in Asian countries.
Xiaoyan Feng,Xin Wen,Ling Li,Zhenchang Sun,Xin Li,Lei Zhang,Jingjing Wu,Xiaorui Fu,Xinhua Wang,Hui Yu,Xinran Ma,Xudong Zhang,Xinli Xie,Xingmin Han,Mingzhi Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3
Purpose There is no optimal prognostic model for T-cell lymphoblastic lymphoma (T-LBL). Here, we discussed the predictive value of total metabolic tumor volume (TMTV) and total lesion glycolysis (TLG) measured on 18F-fluorodeoxyglucose positron emission tomography–computed tomography (PET-CT) in T-LBL.Materials and Methods Thirty-seven treatment naïve T-LBL patients with PET-CT scans were enrolled. TMTV was obtained using the 41% maximum standardized uptake value (SUVmax) threshold method, and TLG was measured as metabolic tumor volume multiplied by the mean SUV. Progression-free survival (PFS) and overall survival (OS) were analyzed by Kaplan-Meier curves and compared by the log-rank test.Results The optimal cutoff values for SUVmax, TMTV, and TLG were 12.7, 302 cm3, and 890, respectively. A high SUVmax, TMTV, and TLG indicated a shorten PFS and OS. On multivariable analysis, TMTV ≥ 302 cm3, and central nervous system (CNS) involvement predicted inferior PFS, while high SUVmax, TLG and CNS involvement were associated with worse OS. Subsequently, we generated a risk model comprising high SUVmax, TMTV or TLG and CNS involvement, which stratified the population into three risk groups, which had significantly different median PFS of not reached, 14 months, and 7 months for low-risk group, mediate-risk group, and high-risk group, respectively (p < 0.001). Median OS were not reached, 27 months, and 13 months, respectively (p < 0.001).Conclusion Baseline SUVmax, TMTV, and TLG measured on PET-CT are strong predictors of worse outcome in T-LBL. A risk model integrating these three parameters with CNS involvement identifies patients at high risk of disease progression.
Hu, Zheng-Hui,Lin, Yi-Wei,Xu, Xin,Chen, Hong,Mao, Ye-Qing,Wu, Jian,Xu, Xiang-Lai,Zhu, Yi,Li, Shi-Qi,Zheng, Xiang-Yi,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Objective: To evaluate the association between tea consumption and the risk of renal cell carcinoma. Methods: We searched PubMed, Web of Science and Scopus between 1970 and November 2012. Two evaluators independently reviewed and selected articles based on predetermined selection criteria. Results: Twelve epidemiological studies (ten case-control studies and two cohort studies) were included in the final analysis. In a meta-analysis of all included studies, when compared with the lowest level of tea consumption, the overall relative risk (RR) of renal cell carcinoma for the highest level of tea consumption was 1.03 (95% confidence interval [CI] 0.89-1.21). In subgroup meta-analyses by study design, there was no significant association between tea consumption and renal cell carcinoma risk in ten case-control studies using adjusted data (RR=1.08, 95% CI 0.84-1.40). Furthermore, there was no significant association in two cohort studies using adjusted data (RR=0.95, 95% CI 0.81-1.12). Conclusion: Our findings do not support the conclusion that tea consumption is related to decreased risk of renal cell carcinoma. Further prospective cohort studies are required.