Rapid Detection of SdhBP225F and SdhBH272R Mutations in Boscalid Resistant Botrytis cinerea Strains by ARMS-PCR

Xin Liu,Rong Zeng,Shigang Gao,Lihui Xu,Fuming Dai 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.1

SdhBP225Fand SdhBH272R mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for SdhBP225F and SdhBH272R were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by SdhBP225F and SdhBH272R mutations.

Effects of the Substitution of Mischmetal for PrNd on the Microstructures and Magnetic Properties of Rapidly Quenched (MM)PrNd-Fe-B Nanocrystalline Magnets

Zeng-ru Zhao,Xin Wang,Xue-feng Zhang,Qiang Ma,Yan-li Liu,Yong-feng Li,Fei Liu,Gao-feng Wang 한국자기학회 2017 Journal of Magnetics Vol.22 No.1

Mischmetal of Bayan Obo ore was utilized to prepare the high performance (Pr7.34Nd21.86)1-xMMxFe68.7Al0.1-Cu0.12Co0.88B ribbons using melt-spinning method. Phase composition and magnetic properties were investigated at room temperature. The ribbons mainly consist of R2Fe14B phase in isotropic nanostructure. Both coercivity and maximum energy product decrease with the increase of MM content. The magnetic parameters of the ribbons with MM = 20 % in mass are Br = 7.38 kGs, Hcj = 13.66 kOe, (BH)max = 11.81 MGOe. Henkel plots were applied to demonstrate the exchange coupling interaction between grains.

Multilevel Pedicle Subtraction Osteotomy for Correction of Thoracolumbar Kyphosis in Ankylosing Spondylitis: Clinical Effect and Biomechanical Evaluation

Xin Lv,Yelidana Nuertai,Qiwei Wang,Di Zhang,Xumin Hu,Jiabao Liu,Ziliang Zeng,Renyuan Huang,Zhihao Huang,Qiancheng Zhao,Wenpeng Li,Zhilei Zhang,Liangbin Gao 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Objective: To compare the clinical outcomes and biomechanical characteristics of 1-, 2-, and 3-level pedicle subtraction osteotomy (PSO), and establish selection criteria based on preoperative radiographic parameters. Methods: Patients undergone PSO to treat ankylosing spondylitis from February 2009 to May 2019 in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled. According to the quantity of osteotomy performed, the participants were divided into group A (1-level PSO, n = 24), group B (2-level PSO, n = 19), and group C (3-level PSO, n = 11). Clinical outcomes were assessed before surgery and at the final follow-up. Comparisons of the radiographic parameters and quality-of-life indicators were performed among and within these groups, and the selection criteria were established by regression. Finite element analysis was conducted to compare the biomechanical characteristics of the spine treated with different quantity of osteotomies under different working conditions. Results: Three-level PSO improved the sagittal parameters more significantly, but resulted in longer operative time and greater blood loss (p < 0.05). Greater stress was found in the proximal screws and proximal junction area of the vertebra in the model simulating 1-level PSO. Larger stress of screws and vertebra was observed at the distal end in the model simulating 3-level PSO. Conclusion: Multilevel PSO works better for larger deformity correction than single-level PSO by allowing greater sagittal parameter correction and obtaining a better distribution of stress in the hardware construct, although with longer operation time and greater blood loss. Three-level osteotomy is recommended for the patients with preoperative of global kyphosis > 85.95°, T1 pelvic angle > 62.3°, sagittal vertical alignment > 299.55 mm, and pelvic tilt+ chin-brow vertical angle > 109.6°.

Rapid Detection of SdhB^P225F and SdhB^H272R Mutations in Boscalid Resistant Botrytis cinerea Strains by ARMS-PCR

Liu, Xin,Zeng, Rong,Gao, Shigang,Xu, Lihui,Dai, Fuming The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.1

$SdhB^{P225F}$ and $SdhB^{H272R}$ mutations have been found associated with boscalid resistance in Botrytis cinerea from strawberry in Shanghai, China. For rapid detection of two mutations, tetra-primers were designed and optimized to gain the relatively high accuracy and specificity based on the ARMS-PCR technique, by which resistance can be identified with different lengths of products on agarose gels. The tetra-primer ARMS-PCR systems for $SdhB^{P225F}$ and $SdhB^{H272R}$ were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by $SdhB^{P225F}$ and $SdhB^{H272R}$ mutations.

2Department of Spine Services, Indian Spinal Injuries Centre, New Delhi, India

Ratish Singh,Gao Zeng Xin,Mangal Prasad Hirachan,Lin Yu Cheng 대한척추외과학회 2018 Asian Spine Journal Vol.12 No.3

Study Design: Retrospective study. Purpose: To evaluate the outcome of percutaneous transforaminal endoscopic lumbar surgery (PTELS) and to evaluate the efficacy of this technique in the treatment of lumbar disc herniation (LDH) and lumbar spine stenosis (LSS) in >60-year-old patients. Overview of Literature: There has been number of studies done in aspect of PTELS. All those studies focused on overall clinical aspect with no any age specific analysis. This articles deals with the patient >60 years old with low back pain (LBP) treated with the PTELS. This study provides the details of clinical outcome after the surgery in those age group. Methods: We retrospectively studied 77 patients aged >60 years; out of these, 45 patients presented with LDH and 22 patients with lumbar spinal stenosis who underwent PTELS. Results: The mean age of the patients who underwent the surgery was 68.33±6.97 years and the mean duration of the surgery and the postoperative duration of hospitalization were 87.31±24.746 minutes and 79±2.711 days, respectively. The pre- and postoperative Oswestry Disability Index were 52.8022±11.98299 and 16.3513±12.97398 (p <0.05), respectively, indicating statistical significance. The pre- and postoperative visual analogue scale scores of the leg and back were 6.27±1.213 and 4.79±1.162 (p <0.05) and 1.40±1.688 and 1.30±1.436, respectively (p <0.05), which showed a significant difference. Based on the MacNab criteria, 76.1%, 10%, and 6% of the patients showed excellent or good, fair, and poor outcomes, respectively. Incomplete removal of content and reherniation were observed in 3% and 7.5% of the patients. No significant difference was found between the clinical outcomes of PTELS between LDH and LSS. Conclusions: PTELS can be a good technique in spine surgery to relieve symptoms for LBP with satisfactory outcome, short duration of hospitalization, and advantages such as elimination of general anesthesia in elderly patients.

Outcomes of Microendoscopic Discectomy and Percutaneous Transforaminal Endoscopic Discectomy for the Treatment of Lumbar Disc Herniation: A Comparative Retrospective Study

Arjun Sinkemani,Xin Hong,Zeng-Xin Gao,Su-Yang Zhuang,Zan-Li Jiang,Shao-Dong Zhang,Jun-Ping Bao,Lei Zhu,Pei Zhang,Xin-Hui Xie,Feng Wang,Xiao-Tao Wu 대한척추외과학회 2015 Asian Spine Journal Vol.9 No.6

Study Design: Retrospective, case control evaluation of 86 patients who underwent microendoscopic discectomy (MED) and percutaneous transforaminal endoscopic discectomy (PTED) for the treatment of lumbar disc herniation (LDH). Purpose: To evaluate the safety and the outcomes of MED and PTED for the treatment of LDH. Overview of Literature: MED and PTED are minimally invasive surgical techniques for lower back pain. Studies to date have shown that MED and PTED are safe and effective treatment modalities for LDH. Methods: A retrospective study was performed in patients with LDH treated with MED (n=50) and transforaminal endoscopic discectomy (PTED; n=36) in our hospital. All patients were followed-up with self-evaluation questionnaires, Oswestry disability index (ODI), medical outcomes study 36-item short form health survey and MacNab criteria. All the patients in both groups were followed up to 12 months after the operation. Results: ODI questionnaire responses were not statistically different between the MED and PTED groups (53.00 vs. 48.72) before treatment. Average scores and minimal disability after 5 days to 12 months of follow-up were 4.96 in the MED group and 3.61 in the PTED group. According to MacNab criteria, 92.0% of the MED group and 94.4% of the PTED group had excellent or good results with no significant difference. Conclusions: There was no significant difference between MED and PTED outcomes. Further large-scale, randomized studies with long-term follow-up are needed.