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The Role of Kif4A in Doxorubicin-Induced Apoptosis in Breast Cancer Cells
Hui Wang,Changqing Lu,Qing Li,Jun Xie,Tongbing Chen,Yan Tan,Changping Wu,Jingting Jiang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.11
This study was to investigate the mechanism and role of Kif4A in doxorubicin-induced apoptosis in breast cancer. Using two human breast cancer cell lines MCF-7 (with wild-type p53) and MDA-MB-231 (with mutant p53), we quantitated the expression levels of kinesin super-family protein 4A (Kif4A) and poly (ADP-ribose) Polymerase-1 (PARP-1) by Western blot after doxorubicin treatment and examined the apoptosis by flow cytometry after treatment with doxorubicin and PARP-1 inhibitor, 3-Aminobenzamide (3-ABA). Our results showed that doxorubicin treatment could induce the apoptosis of MCF-7 and MDA-MB-231 cells, the down-regulation of Kif4A and upregulation of poly(ADP-ribose) (PAR). The activity of PARP-1 or PARP-1 activation was significantly elevated by doxorubicin treatment in dose- and time-dependent manners (P < 0.05), while doxorubicin treatment only slightly elevated the level of cleaved fragments of PARP-1 (P > 0.05). We further demonstrated that overexpression of Kif4A could reduce the level of PAR and significantly increase apoptosis. The effect of doxorubicin on apoptosis was more profound in MCF-7 cells compared with MDA-MB-231 cells (P < 0.05). Taken together, our results suggest that the novel role of Kif4A in doxorubicin-induced apoptosis in breast cancer cells is achieved by inhibiting the activity of PARP-1.
Zhao-Hui Jiang,Yong-Fang Xie,Wei-Hua Gui,Can Huang 제어·로봇·시스템학회 2010 International Journal of Control, Automation, and Vol.8 No.1
In this paper, decentralized robust H∞ output feedback control problem for large-scale interconnected system with value bounded uncertainties in the state, control input and interconnection matrices is investigated. A new bounded real lemma for the large-scale interconnected systems is derived by Lyapunov stability theory and linear matrix inequality method. Based on the new bounded real lemma, a sufficient condition expressed as matrix inequalities for the existence of a decentralized robust H∞ output feedback controller is obtained. The controller which enables the closed-loop large-scale system robust stable and satisfies the given H∞ performance is designed through a homotopy iterative method. Finally, a numerical example is given to illustrate the effectiveness of the proposed method.
The Role of Kif4A in Doxorubicin-Induced Apoptosis in Breast Cancer Cells
Wang, Hui,Lu, Changqing,Li, Qing,Xie, Jun,Chen, Tongbing,Tan, Yan,Wu, Changping,Jiang, Jingting Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.11
This study was to investigate the mechanism and role of Kif4A in doxorubicin-induced apoptosis in breast cancer. Using two human breast cancer cell lines MCF-7 (with wild-type p53) and MDA-MB-231 (with mutant p53), we quantitated the expression levels of kinesin super-family protein 4A (Kif4A) and poly (ADP-ribose) Polymerase-1 (PARP-1) by Western blot after doxorubicin treatment and examined the apoptosis by flow cytometry after treatment with doxorubicin and PARP-1 inhibitor, 3-Aminobenzamide (3-ABA). Our results showed that doxorubicin treatment could induce the apoptosis of MCF-7 and MDA-MB-231 cells, the down-regulation of Kif4A and upregulation of poly(ADP-ribose) (PAR). The activity of PARP-1 or PARP-1 activation was significantly elevated by doxorubicin treatment in dose- and time-dependent manners (P < 0.05), while doxorubicin treatment only slightly elevated the level of cleaved fragments of PARP-1 (P > 0.05). We further demonstrated that overexpression of Kif4A could reduce the level of PAR and significantly increase apoptosis. The effect of doxorubicin on apoptosis was more profound in MCF-7 cells compared with MDA-MB-231 cells (P < 0.05). Taken together, our results suggest that the novel role of Kif4A in doxorubicin-induced apoptosis in breast cancer cells is achieved by inhibiting the activity of PARP-1.
Yong-Fang Xie,Wei-Hua Gui,Zhao-Hui Jiang 대한전기학회 2008 International Journal of Control, Automation, and Vol.6 No.4
In this paper, the problem of delay-dependent stabilization for singular systems with multiple internal and external incommensurate constant point delays is investigated. The condition when a singular system subject to point delays is regular independent of time delays is given and it can be easily test with numerical or algebraic methods. Based on Lyapunov-Krasovskii functional approach and the descriptor integral-inequality lemma, a sufficient condition for delay-dependent stability is obtained. The main idea is to design multiple memory less state feedback control laws such that the resulting closed-loop system is regular independent of time delays, impulse free, and asymptotically stable via solving a strict linear matrix inequality (LMI) problem. An explicit expression for the desired memory less state feedback control laws is also given. Finally, a numerical example illustrates the effectiveness and the availability for the proposed method.
Qian Ding,Xiao-Li Xie,Miao-Miao Wang,Jie Yin,Jin-Mei Tian,Xiao-Yu Jiang,Di Zhang,Jing Han,Yun Bai,Zi-Jin Cui,Hui-Qing Jiang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
The clearance of activated hepatic stellate cells (HSCs) by apoptosis is critical for the reversibility of hepatic fibrosis. Mitochondrial homeostasis is regulated by mitophagy, which is an efficient way of clearing injured mitochondria that plays an important role in apoptosis. However, the role of mitophagy in apoptosis in HSCs and hepatic fibrosis is still unclear. Here, we show that mitophagy is enhanced in parallel with increased apoptosis in hepatic stellate cells during the reversal of hepatic fibrosis. The inhibition of mitophagy suppressed apoptosis in HSCs and aggravated hepatic fibrosis in mice. In contrast, the activation of mitophagy induced apoptosis in HSCs. Furthermore, we confirmed that BCL-B, which is a member of the BCL-2 family, is a regulator mediating mitophagy-related apoptosis. The knockdown of BCL-B resulted in increased apoptosis and mitophagy in HSCs, while the overexpression of BCL-B caused the opposite effects. BCL-B inhibited the phosphorylation of Parkin (a key regulator of mitophagy) and directly bound phospho-Parkin. Altogether, enhanced mitophagy promotes apoptosis in HSCs during the reversal of hepatic fibrosis. BCL-B suppresses mitophagy in HSCs by binding and suppressing phospho-Parkin, thereby inhibiting apoptosis. BCLB- dependent mitophagy is a new pathway for the regulation of apoptosis in HSCs during the regression of hepatic fibrosis
Proposals for flexural capacity prediction method of externally prestressed concrete beam
Wu-Tong Yan,Liang-Jiang Chen,Bing Han,Feng Wei,Hui-Bing Xie,Jia-Ping Yu 국제구조공학회 2022 Structural Engineering and Mechanics, An Int'l Jou Vol.83 No.3
Flexural capacity prediction is a challenging problem for externally prestressed concrete beams (EPCBs) due to the unbonded phenomenon between the concrete beam and external tendons. Many prediction equations have been provided in previous research but typically ignored the differences in deformation mode between internal and external unbonded tendons. The availability of these equations for EPCBs is controversial due to the inconsistent deformation modes and ignored secondorder effects. In this study, the deformation characteristics and collapse mechanism of EPCB are carefully considered, and the ultimate deflected shape curves are derived based on the simplified curvature distribution. With the compatible relation between external tendons and the concrete beam, the equations of tendon elongation and eccentricity loss at ultimate states are derived, and the geometric interpretation is clearly presented. Combined with the sectional equilibrium equations, a rational and simplified flexural capacity prediction method for EPCBs is proposed. The key parameter, plastic hinge length, is emphatically discussed and determined by the sensitivity analysis of 324 FE analysis results. With 94 collected laboratory-tested results, the effectiveness of the proposed method is confirmed, and comparisons with the previous formulas are made. The results show the better prediction accuracy of the proposed method for both stress increments and flexural capacity of EPCBs and the main reasons are discussed.
( Lin Zhou ),( Yan Zhong ),( Fang Hui Yang ),( Zi Bo Li ),( Jiang Zhou ),( Xie Hong Liu ),( Min Li ),( Fang Hu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2016 BMB Reports Vol.49 No.3
Kaiso is a Pox Virus and Zinc Finger (POZ-ZF) transcription factor with bi-modal DNA-binding specificity. Here, we demonstrated that Kaiso expression is inversely correlated with glucocorticoid receptor (GR) expression in breast carcinomas. Knockdown of Kaiso increased GR expression, while overexpression of Kaiso inhibited GR expression in breast cancer cells. Furthermore, Kaiso repressed GR proximal promoter-reporter activity in a dose-dependent manner. Remarkably, ChIP experiments demonstrated that endogenous Kaiso was associated with the GR promoter sequence in a methylation-dependent manner. Since glucocorticoids inhibit chemotherapyinduced apoptosis and have been widely used as a co-treatment of patients with breast cancer, we assessed the role of Kasio in GR-mediated anti-apoptotic effects. We found that overexpression of Kaiso attenuated the anti-apoptotic effects of glucocorticoids in breast cancer cells. Our findings suggest that GR is a putative target gene of Kaiso and suggest Kaiso to be a potential therapeutic target in GC-combination chemotherapy in breast cancer. [BMB Reports 2016; 49(3): 167-172]
Arjun Sinkemani,Xin Hong,Zeng-Xin Gao,Su-Yang Zhuang,Zan-Li Jiang,Shao-Dong Zhang,Jun-Ping Bao,Lei Zhu,Pei Zhang,Xin-Hui Xie,Feng Wang,Xiao-Tao Wu 대한척추외과학회 2015 Asian Spine Journal Vol.9 No.6
Study Design: Retrospective, case control evaluation of 86 patients who underwent microendoscopic discectomy (MED) and percutaneous transforaminal endoscopic discectomy (PTED) for the treatment of lumbar disc herniation (LDH). Purpose: To evaluate the safety and the outcomes of MED and PTED for the treatment of LDH. Overview of Literature: MED and PTED are minimally invasive surgical techniques for lower back pain. Studies to date have shown that MED and PTED are safe and effective treatment modalities for LDH. Methods: A retrospective study was performed in patients with LDH treated with MED (n=50) and transforaminal endoscopic discectomy (PTED; n=36) in our hospital. All patients were followed-up with self-evaluation questionnaires, Oswestry disability index (ODI), medical outcomes study 36-item short form health survey and MacNab criteria. All the patients in both groups were followed up to 12 months after the operation. Results: ODI questionnaire responses were not statistically different between the MED and PTED groups (53.00 vs. 48.72) before treatment. Average scores and minimal disability after 5 days to 12 months of follow-up were 4.96 in the MED group and 3.61 in the PTED group. According to MacNab criteria, 92.0% of the MED group and 94.4% of the PTED group had excellent or good results with no significant difference. Conclusions: There was no significant difference between MED and PTED outcomes. Further large-scale, randomized studies with long-term follow-up are needed.