Relay Protection Sport Systems Based on MATLAB-Based Simulation Technology

Xiaojun He 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.1

MATLAB-based simulation technology can support the analysis and design of relay protection sport systems. A simulation model is built for the study of sport system relay protection. As an example, the sport system fault simulation, zero-sequence current protection simulation and transformer differential protection simulation are presented herein. The case studies show that the simulation of sport system relay protection is effective and feasible.

A Novel Xylanase from Streptomyces sp. FA1: Purification, Characterization, Identification, and Heterologous Expression

Jie He,Lingqia Su,Xiaojun Sun,Jiajia Fu,Jian Chen,Jing Wu 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

A xylanase (XynA) was purified from the culturemedium of Streptomyces sp. FA1, which was previouslyisolated from a bamboo retting system. XynA had amolecular mass of 43 kDa, displayed maximal activity atpH 5.5, retained 41% of its maximal activity at pH 11.0,and was stable over a wide pH range (3.0 ~ 11.0). PurifiedXynA was subjected to peptide mass fingerprinting, whichled to the cloning of the xynA gene. The xynA gene, whichencodes a mature protein of 436 amino acids, washeterologously expressed in E. coli BL21(DE3). The activityin the culture medium could reach 213.5 U/mL, which was11.2-fold higher than that produced by Streptomyces sp. FA1. BLAST searching revealed that full-length XynAshares less than 90% identity with most of its homologues,whereas amino acids 48-436 of the enzyme share 97%identity with an open reading frame encoding a putativefull-length mature xylanase from Streptomyces tendae. Thetruncated xynA gene, xynA48-436, was cloned and expressedin E. coli, however, no xylanase activity could be detectedin the culture medium. Based on these results, it is suggestedthat XynA is a new member of glycoside hydrolasesfamily10 with exceptional catalytic efficiency at alkalinepH.

The Prognostic Significance of Notch1 and Fatty Acid Binding Protein 7 (FABP7) Expression in Resected Tracheobronchial Adenoid Cystic Carcinoma: A Multicenter Retrospective Study

Mian Xie,Xiaojun Wu,Jinjun Zhang,Chaosheng He,Shenhai Wei,Junyao Huang,Xinge Fu,Yingying Gu 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4

Purpose Adenoid cystic carcinoma (ACC) of the trachea and bronchus is a rare tumor. Although MYBNFIB oncogene fusion and Notch1 mutation have been identified in ACC, little is known about the expression and clinical significance of Notch1 and its target gene fatty acid binding protein 7 (FABP7) in tracheobronchial ACC. Materials and Methods Primary tracheobronchial ACC that were resected between 1998 and 2014 were identified through the pathology and oncology database from five thoracic oncology centers in China. A tissue array was constructed from the patients’ samples and the expressions of Notch1 and FABP7 were evaluated by immunohistochemistry. The association between the expression of both markers and survival was determined. Results Overexpression of Notch1 and FABP7, detected in 37.8% and 38.3% of 368 patients with tracheobronchial ACC, respectively, was an independent prognostic indicator for recurrencefree survival (RFS) by multivariable Cox proportional hazard model (p=0.032 and p=0.048, respectively). Overexpression of Notch1, but not of FABP7, predicted overall survival (OS) (p=0.018). When categorized into four groups according to coexpression of Notch1 and FABP7, patients with overexpression of both Notch1 and FABP7 belonged to the group with the shortest RFS and OS (p=0.01 and p=0.048, respectively). Conclusion Expression of Notch1 and FABP7, and coexpression of Notch1 and FABP7, is strongly associated with poor survival in resected tracheobronchial ACC. These data are consistent with the hypothesis that poor differentiation of tracheobronchial ACC correlates with the activation of Notch signaling.

Genome-wide identification and evolutionary analysis of WOX gene family in cucurbit crops

Li Chun,He Zhen,Liang Genyun,Yang Nan,Cai Peng,Liang Ying,Li Yuejian,Fang Chao,Liu Duchen,Xia Feng,Liu Xiaojun 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.1

The WUSCHEL-related homeobox (WOX) gene family is essential for plant growth and development. However, the identifi cation and evolutionary analysis of WOX genes in the Cucurbitaceae have not been well investigated. In the present work, we performed a genome-wide analysis to identify WOX genes in eleven cucurbit crops. In total, 127 WOX genes were identifi ed in eleven cucurbit crops, and classifi ed into three diff erent clades: the ancient clade, the intermediate clade and the WUS clade. The same clade has comparable gene organization and conserved motif patterns. The cis -acting ele ment analysis revealed that WOX genes were involved in light, hormone and stress in cucurbit crops. Gene duplication analysis indicated that dispersed duplication and whole genome duplication were the two primary causes of WOX genes expansion. There were 24 and 129 gene pairs found within cucurbit crops, and between Cucumis sativus and other cucur bit crops, respectively. After gene duplication, all WOX genes were subjected to substantial purifying selection pressure according to the selective pressure analysis. Functional divergence analysis revealed that ancient and intermediate clades may have descended from some essential amino acid sites rather than diff erent evolution rate. Our research provides a comprehensive analysis of WOX genes which will be helpful for further research into their function study in Cucurbita ceae plants.

D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation

Bo Qiao,Bin He,Ao Zhou,Yuling Li,Xiaojun Chen,Qiming Yang,Beike Chen,Bo Qiao,Dianming Jiang 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.2

BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (DRADA16- RGD sequence containing all D-amino acids is Ac-RADARADARADARADARGDS-CONH2) shows admirable bioactivity for both cell culture and bone regeneration. Here, we describe the fabrication of a favorable biomaterial material (nHA/PA66/D-RADA16-RGD). METHODS: Proteinase K and circular dichroism spectroscopy were employed to test the stability and secondary structural properties of peptide D-RADA16-RGD respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the surface of these materials. Confocal laser scanning (CLS), cell counting kit-8 tests (CCK-8), alizarin red S staining, cell immunofluorescence analysis and Western blotting were involved in vitro. Also biosafety and bioactivity of them have been evaluated in vivo. RESULTS: Proteinase K and circular dichroism spectroscopy demonstrated that D-RADA16-RGD in nHA/PA66 was able to form stable-sheet secondary structure. SEM and TEM showed that the D-RADA16-RGD material was 7–33 nm in width and 130–600 nm in length, and the interwoven pore size ranged from 40 to 200 nm. CLS suggests that cells in nHA/PA66/ D-RADA16-RGD group were linked to adjacent cells with more actin filaments. CCK-8 analysis showed that nHA/PA66/ D-RADA16-RGD revealed good biocompatibility. The results of Alizarin-red S staining and Western blotting as well as vivo osteogenesis suggest nHA/PA66/D-RADA16-RGD exhibits better bioactivity. CONCLUSION: This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation.

Aerodynamic properties of a streamlined bridge-girder under the interference of trains

Huan Li,Xu-hui He,Liang Hu,Xiaojun Wei 한국풍공학회 2022 Wind and Structures, An International Journal (WAS Vol.35 No.3

Trains emerging on a streamlined bridge-girder may have salient interference effects on the aerodynamic properties of the bridge. The present paper aims at investigating these interferences by wind tunnel measurements, covering surface pressure distributions, near wake profiles, and flow visualizations. Experimental results show that the above interferences can be categorized into two primary effects, i.e., an additional angle of attack (AoA) and an enhancement in flow separation. The additional AoA effect is demonstrated by the upward-moved stagnation point of the oncoming flow, the up-shifted global symmetrical axis of flow around the bridge-girder, and the clockwise-deflected orientation of flow approaching the bridgegirder. Due to this additional AoA effect, the two critical AoAs, where flow around the bridge-girder transits from trailing-edge vortex shedding (TEVS) to impinging leading-edge vortices (ILEV) and from ILEV to leading-edge vortex shedding (LEVS) of the bridge-girder are increased by 4° with respect to the same bridge-girder without trains. On the other hand, the underlying flow physics of the enhancement in flow separation is the large-scale vortices shedding from trains instead of TEVS, ILEV, and LEVS governed the upper half bridge-girder without trains in different ranges of AoA. Because of this enhancement, the mean lift and moment force coefficients, all the three fluctuating force coefficients (drag, lift, and moment), and the aerodynamic spanwise correlation of the bridge-girder are more significant than those without trains.

An Optimal Combined SVM Model for Short-term Wind Speed Forecasting

Bai, Dan-Dan,He, Jing-Han,Tian, Wen-Qi,Wang, Xiaojun,Tony, Yip 대한전기학회 2014 The Journal of International Council on Electrical Vol.4 No.4

A high precise wind speed forecasting method is one of current wind power research hotspots. This paper presented a combined wind speed forecasting model based on support vector machine (SVM) optimized by particle swarm optimization (PSO) using historical data of wind speed at the site. The model took the results of back propagation neural network (BPNN), radial basis function neural network (RBFNN), genetic neural network (GNN) and wavelet neural network (WNN) as the inputs, and adopted the actual wind speed as the output. Meanwhile, particle swarm optimization was used to optimize model parameters. Apply this model in hourly prediction of wind speed using historical data from a wind farm in Shanxi Province. It is observed that its prediction accuracy was not only higher than that of any of its single network but higher than traditional linear combined forecasting model and neural network combined forecasting model.

Macro-/micro-environment-sensitive chemosensing and biological imaging

Yang, Zhigang,Cao, Jianfang,He, Yanxia,Yang, Jung Ho,Kim, Taeyoung,Peng, Xiaojun,Kim, Jong Seung The Royal Society of Chemistry 2014 Chemical Society reviews Vol.43 No.13

<P>Environment-related parameters, including viscosity, polarity, temperature, hypoxia, and pH, play pivotal roles in controlling the physical or chemical behaviors of local molecules. In particular, in a biological environment, such factors predominantly determine the biological properties of the local environment or reflect corresponding status alterations. Abnormal changes in these factors would cause cellular malfunction or become a hallmark of the occurrence of severe diseases. Therefore, in recent years, they have increasingly attracted research interest from the fields of chemistry and biological chemistry. With the emergence of fluorescence sensing and imaging technology, several fluorescent chemosensors have been designed to respond to such parameters and to further map their distributions and variations <I>in vitro</I>/<I>in vivo</I>. In this work, we have reviewed a number of various environment-responsive chemosensors related to fluorescent recognition of viscosity, polarity, temperature, hypoxia, and pH that have been reported thus far.</P> <P>Graphic Abstract</P><P>We have summarized the research progress on fluorescent sensors responsive to environmental factors, including local viscosity, polarity, temperature, hypoxia and pH. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cs00051j'> </P>

Automatic Subretinal Fluid Segmentation of Retinal SD-OCT Images With Neurosensory Retinal Detachment Guided by Enface Fundus Imaging

Wu, Menglin,Chen, Qiang,He, XiaoJun,Li, Ping,Fan, Wen,Yuan, SongTao,Park, Hyunjin IEEE 2018 IEEE Transactions on Biomedical Engineering Vol.65 No.1

<P>Objective: Accurate segmentation of neurosensory retinal detachment (NRD) associated subretinal fluid in spectral domain optical coherence tomography (SD-OCT) is vital for the assessment of central serous chorioretinopathy (CSC). A novel two-stage segmentation algorithm was proposed, guided by Enface fundus imaging. Methods: In the first stage, Enface fundus image was segmented using thickness map prior to detecting the fluid-associated abnormalities with diffuse boundaries. In the second stage, the locations of the abnormalities were used to restrict the spatial extent of the fluid region, and a fuzzy level set method with a spatial smoothness constraint was applied to subretinal fluid segmentation in the SD-OCT scans. Results: Experimental results from 31 retinal SD-OCT volumes with CSC demonstrate that our method can achieve a true positive volume fraction (TPVF), false positive volume fraction (FPVF), and positive predicative value (PPV) of 94.3%, 0.97%, and 93.6%, respectively, for NRD regions. Our approach can also discriminate NRD-associated subretinal fluid from subretinal pigment epithelium fluid associated with pigment epithelial detachment with a TPVF, FPVF, and PPV of 93.8%, 0.40%, and 90.5%, respectively. Conclusion: We report a fully automatic method for the segmentation of subretinal fluid. Significance: Our method shows the potential to improve clinical therapy for CSC.</P>

An Efficient Dynamic Group Signature with Non-frameability

( Run Xie ),( Chunxiang Xu ),( Chanlian He ),( Xiaojun Zhang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.5

A group signature scheme allows any member to sign on behalf of a group. It is applied to practical distributed security communication environments, such as privacy-preserving, data mining. In particular, the excellent features of group signatures, including membership joining and revocation, anonymity, traceability, non-frameability and controllable linkability, make group signature scheme more attractive. Among these features, non-frameability can guarantee that a member`s signature cannot be forged by any other (including issuer), and controllable linkability supports to confirm whether or not two group signatures are created by the same signer while preserving anonymity. Until now, only Hwang et al.`s group schemes (proposed in 2013 and 2015) can support all of these features. In this paper, we present a new dynamic group signature scheme which can achieve all of the above excellent features. Compared with their schemes, our scheme has the following advantages. Firstly, our scheme achieves more efficient membership revocation, signing and verifying. The cost of update key in our scheme is two-thirds of them. Secondly, the tracing algorithm is simpler, since the signer can be determined without the judging step. Furthermore, in our scheme, the size of group public key and member`s private key are shorter. Lastly, we also prove security features of our scheme, such as anonymity, traceability, non-frameability, under a random oracle model.