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Microstructural Evolution of TP347H upon Long-Term Service in Power Plants
Fang, Chao,He, Yin Sheng,Yoo, Keun Bong,Jung, Jine Sung,Shin, Kee Sam Trans Tech Publications, Ltd. 2017 Key Engineering Materials Vol.727 No.-
<P>Effects of welding and long-term service on the microstructural evolution of superheater tubes of TP347H stainless steel used in power plants were investigated by optical microscope (OM), scanning electron microscope (SEM), electron back-scattered diffraction (EBSD), and transmission electron microscope (TEM). Analyses after welding or long-term service, showed fine NbCs in grains, which will precipitaion strengthen the matrix. When TP347 was long-term serviced in power plants, M23C6 formed preferentially on the grain boundaries and on twin boundaries, which was attributed to the embrittlement and the intergranular corrosion and fracture. The steam side had less recrystallization rate and more oxide compared to the fire side, which is part of the reason for the cracking from steam side to the fire side. And HAZ is more brittle than the matrix, because of α-Fe phase and coarse grains, due to which, cracks tend to initiate in the steam side of HAZ and propagate to the fire side.</P>
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SVM model for Predicting Human Proteins Interacting with HCV Proteins
방초(Chao Fang),최광우(Guangyu Cui),한경숙(Kyungsook Han) 한국정보과학회 2011 정보과학회 컴퓨팅의 실제 논문지 Vol.17 No.4
단백질 상호작용을 예측하기 위하여 몇몇 계산 학적 방법들이 개발되었으나, 대부분 한 종류의 생명체에서의 단백질 상호작용에 국한된 것이다. 동종 단백질간의 상호작용 예측 기법은 단백질 종류를 구별하지 않기 때문에 이종 단백질간의 상호작용을 예측하는데 적합하지 않다. 본 논문은 단백질 서열 데이터를 이용하여C형 간염 바이러스 (HCV) 단백질과 인간 단백질의 상호작용을 예측하는 support vector machine (SVM) 모델의 개발을 소개한다. 이 SVM 모델의 예측 정확도는 평균적으로 81.5%임을 보였다. 이 SVM 모델과 단백질의 유전자 온톨로지 정보를 이용하여, HCV단백질과 인간 단백질 사이의 새로운 상호작용을 456개 예측하였다. Several computational methods have been developed for predicting protein-protein interactions, but most of these methods are intended for finding the protein-protein interactions within a species rather than for the interactions across different species. Methods for predicting the interactions between homogeneous proteins are not appropriate for predicting the interactions between heterogeneous proteins since they do not distinguish the interactions between proteins of the same species from those of different species. In this paper we present the development of a support vector machine (SVM) model that predicts the interactions between hepatitis C virus (HCV) proteins and human proteins using the sequence data. The average accuracy of the SVM model in predicting the interactions between HCV proteins and human proteins is 81.5%. Using the SVM model and the Gene Ontology (GO) annotations of proteins, we also predicted a total of 456 new interactions between HCV and human proteins.
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Zhao, Chao Fang,Komine, H.,Akiyama, M.,Sugimori, Y.,Matsuura, N. 대한원격탐사학회 1996 International Symposium on Remote Sensing Vol.12 No.1
The WAM (WAve Modeling) model, as one of the best wave models used in the world, has been used for wave data assimilation, SAR data verification and wave forecasting on global and regional scales. In this study, an experiment of the wave model comparison is carried out around Japan Island by using WAM wave model and Japan Meteorological Agency wave model (JWA3G). Both of these two model results are compared with the Ocean Buoy Station around Japan, which are operated by Japan Meteorological Agency. Compared with WAM model, wind input and dissipation have been modified in JWA3G model. Wind input data used in both wave model are the interpolated result with 0.5x0.5 degree grid by using ECMWF wind data with spatial resolution of 2.5x2.5 degree. The main results can be summaried as follows, (I). Compared with buoy data, the predicted wave height from both models are delayed several hours in typhoon period. This result is consistent with wind data difference between buoy data and the predicted wind data by ECMWF. (2). The predicted wave height by both models give a consistent result around typhoon period. (3). In low sea state, the result from WAM model gives a much better result than that from JWA3G model by comparing with buoy data.
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Shu-ting Liu,Chao Zhan,Yun-jing Ma,Chao-yang Guo,Wei Chen,Xiao-ming Fang,Lei Fang 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.-
Background There are several effective complementary and integrative therapies for patients with severe COVID-19. The trial aims to evaluate the efficacy and advantages of the qigong exercise and acupressure rehabilitation program (QARP) for treating patients with severe COVID-19. Methods A total of 128 patients with COVID-19 aged 20 to 80 years were recruited and randomly allocated in a 1:1 ratio to receive QARP plus standard therapies or standard therapies alone. QARP consisted of acupressure therapy and qigong exercise (Liu Zi Jue). The primary outcome was measured with the modified Medical Research Council (mMRC) dyspnea scale, and the secondary outcomes included the modified Borg dyspnea scale (MBS), fatigue Scale-14 (FS-14), patient health questionnaire-9 scale (PHQ-9), duration of respiratory symptoms, and vital signs. Results In total, 128 patients completed the clinical trial. The QARP group and standard therapies group showed significant improvements in vital signs (except blood pressure) and clinical scales compared with baseline (p<0.05). The QARP group also showed more significant improvement in the mMRC dyspnea scale (-1.8 [-2.1, -1.6], p=0.018) and modified Borg dyspnea scale (-3.7 [95% confidence intervals (CI) -4.3, -3.1], p=0.045). The duration of cough was 14.3 days (95% CI 12.6, 16.1, p=0.046), and the length of hospital stay was 18.5 days (95% CI 17.0, 20.0, p=0.042) in the QARP group, both of which were significantly reduced compared with the standard therapies group (p<0.05). Conclusion QARP plus standard therapies improved lung function and symptoms such as dyspnea and cough in patients with severe COVID-19 and shortened the length of hospital stay. Therefore, QARP may be considered an effective treatment option for patients with severe COVID-19. Trial registration Clinical Research Information Service Identifier: ChiCTR2000029994 Background There are several effective complementary and integrative therapies for patients with severe COVID-19. The trial aims to evaluate the efficacy and advantages of the qigong exercise and acupressure rehabilitation program (QARP) for treating patients with severe COVID-19. Methods A total of 128 patients with COVID-19 aged 20 to 80 years were recruited and randomly allocated in a 1:1 ratio to receive QARP plus standard therapies or standard therapies alone. QARP consisted of acupressure therapy and qigong exercise (Liu Zi Jue). The primary outcome was measured with the modified Medical Research Council (mMRC) dyspnea scale, and the secondary outcomes included the modified Borg dyspnea scale (MBS), fatigue Scale-14 (FS-14), patient health questionnaire-9 scale (PHQ-9), duration of respiratory symptoms, and vital signs. Results In total, 128 patients completed the clinical trial. The QARP group and standard therapies group showed significant improvements in vital signs (except blood pressure) and clinical scales compared with baseline (p<0.05). The QARP group also showed more significant improvement in the mMRC dyspnea scale (-1.8 [-2.1, -1.6], p=0.018) and modified Borg dyspnea scale (-3.7 [95% confidence intervals (CI) -4.3, -3.1], p=0.045). The duration of cough was 14.3 days (95% CI 12.6, 16.1, p=0.046), and the length of hospital stay was 18.5 days (95% CI 17.0, 20.0, p=0.042) in the QARP group, both of which were significantly reduced compared with the standard therapies group (p<0.05). Conclusion QARP plus standard therapies improved lung function and symptoms such as dyspnea and cough in patients with severe COVID-19 and shortened the length of hospital stay. Therefore, QARP may be considered an effective treatment option for patients with severe COVID-19. Trial registration Clinical Research Information Service Identifier: ChiCTR2000029994
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Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China
Wei-Feng Qian,Wen-Chao Yan,Tian-Qi Wang,Kai Zhai,Li-Fang Han,Chao-Chao Lv 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1
The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3’+5’) SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29- 2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.
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( Chao Tong ),( Ning Chen ),( Xun Liao ),( Xuemei Yuan ),( Mengjiao Sun ),( Xiaoliang Li ),( Weihuan Fang ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.9
Classical swine fever virus (CSFV) is the etiologic agent of classical swine fever, a highly contagious disease that causes significant economic losses to the swine industry. The lapinized C-strain, a widely used vaccine strain against CSFV, has low growth efficiency in cell culture, which limits the productivity in the vaccine industry. In this study, a recombinant virus derived from C-strain was constructed and subjected to continuous passaging in PK-15 cells with the goal of acquiring a high progeny virus yield. A cell-adapted virus variant, RecCpp80, had nearly 1,000-fold higher titer than its parent C-strain but lost the ability to induce fever in rabbits. Sequence analysis of cell-adapted RecC variants indicated that at least six nucleotide changes were fixed in RecCpp80. Further adaption of RecCpp80 variant in swine testicle cells led to a higher virus yield without additional mutations. Introduction of each of these residues into the wild-type RecC backbone showed that one mutation, M979R (T3310G), located in the C-terminal region of E2 might be closely related to the cell-adapted phenotype. Rabbit inoculation revealed that RecCpp80+10 failed to induce fever in rabbits, whereas RecCpp40<sub>+10</sub> caused a fever response similar to the commercial C-strain vaccine. In conclusion, the C-strain can be adapted to cell culture by introducing specific mutations in its E2 protein. The mutations in RecCpp80 that led to the loss of fever response in rabbits require further investigation. Continuous passaging of the C-strain-based recombinant viruses in PK-15 cells could enhance its in vitro adaption. The non-synonymous mutations at 3310 and 3531 might play major roles in the enhanced capacity of general virus reproduction. Such findings may help design a modified C-strain for improved productivity of commercial vaccines at reduced production cost.
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The acrosswind response of the downwind prism in a twin-prism system with a staggered arrangement
Fang, Fuh-Min,Chung, Cheng-Yang,Li, Yi-Chao,Liu, Wen-Chin,Lei, Perng-Kwei Techno-Press 2013 Wind and Structures, An International Journal (WAS Vol.17 No.3
The flow interaction between two identical neighboring twin square prisms in a staggered arrangement in an open terrain was investigated experimentally. The downwind prism was mounted on a rigid-aeroelastic setup in an open-terrain boundary layer flow to measure its acrosswind root-mean-square responses and aerodynamic damping ratios. By varying the relative location of the upwind prism and the Scruton number associated with the downwind prism, the acrosswind aeroelastic behavior of the downwind prism was analyzed and compared to that of an isolated one. Results showed that the acrosswind root-mean-square response of the downwind prism could be either suppressed or enhanced by the wake flow produced by the neighboring upwind prism. Besides the assessment of the wake effect of the downwind prism, finally, regressed relationships were presented to describe the variation of the aerodynamic damping ratio so as to predict its acrosswind fluctuating response numerically.
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