Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita

Wenjie Tong(Wenjie Tong),Junying Li(Junying Li),Wenfeng Cong(Wenfeng Cong),Cuiping Zhang(Cuiping Zhang),Zhaoli Xu(Zhaoli Xu),Xiaolong Chen(Xiaolong Chen),Min Yang(Min Yang),Jiani Liu(Jiani Liu),Lei Yu 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

Promotional effect of Ce in NH3-SCO and NH3-SCR reactions over Cu-Ce/ SCR catalysts

Wenjie Liu,Yifei Long,Shinian Liu,Yongyan Zhou,XinTong,Yajie Yin,Xiaoyi Li,Kang Hu,Jiangjun Hu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

Commercial V-W-Ti catalysts were modified with Cu and different contents of Ce to remove slip ammoniaand remaining nitrogen oxide from the end stage of SCR technology. The activities of NH3 oxidation byO2 (NH3-SCO) and NO reduction by NH3 (NH3-SCR) of these catalysts were examined from 150 to 400 ℃. The consequences indicated that the addition of Ce and Cu could considerably enhance NH3 oxidation andNO reduction capacity of the catalysts and Ce was conducive to reduce the detrimental effects of H2O andSO2. The characterization tests indicated that the surface active oxygen increased because of the interactionbetween V, Cu and Ce. Moreover, the loaded Ce and Cu contributed to enhance the surface acidityand redox property of SCR catalysts.

Modified Quasi-Z-Source Inverter with Model Predictive Control for Constant Common-Mode Voltage

Wenjie Liu,Yongheng Yang,Tamas Kerekes 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The common-mode voltage (CMV) of transformerless inverters in photovoltaic system should be properly addressed. The conventional CMV reduction methods for voltage source inverters should be modified when applied to the quasi-Z-source inverters with shoot-through states. The shoot-through states in a quasi-Z-source inverter may lead to high-frequency harmonics in the CMV, thus inducing severe leakage currents. Hence, this paper analyzes the CMV in the quasi-Z-source inverter. More importantly, a modified quasi-Z-source inverter that can achieve a constant CMV through model predictive control is discussed. Furthermore, redundant switching vectors are added to reduce the computation burden when the model predictive control is adopted. Simulations are presented to validate the CMV reduction methods.

Energy Landscape Paving with Local Search for Global Optimization of the BLN Off-lattice Model

Jingfa Liu,Weibo Huang,Wenjie Liu,Yuanyuan Sun,Beibei Song,Mao Chen 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.4

The optimization problem for finding the global minimum energy structure is one of the mainproblems of protein structure prediction and is known to be an NP-hard problem in computationalmolecular biology. The low-energy conformational search problem in the hydrophobic-hydrophilicneutral(BLN) off-lattice model is studied. We convert the problem into an unconstrained optimizationproblem by introducing the penalty function. By putting forward a new updating mechanismof the histogram function in the energy landscape paving (ELP) method and incorporating heuristicconformation update strategies into the ELP method, we obtain an improved ELP (IELP) method. Subsequently, by combining the IELP method with the local search (LS) based on the gradient descentmethod, we propose a hybrid algorithm, denoted by IELP-LS, for the conformational searchof the off-lattice BLN model. Simulation results indicate that IELP-LS can find lower-energy statesthan other methods in the literature, showing that the proposed method is an effective tool forglobal optimization in the BLN off-lattice protein model.

Chondrogenic Differentiation and Three Dimensional Chondrogenesis of Human Adipose-Derived Stem Cells Induced by Engineered Cartilage-Derived Conditional Media

Hengyun Sun,Yu Liu,Ting Jiang,Xia Liu,Aijuan He,Jie Li,Wenjie Zhang,Wei Liu,Yilin Cao,Guangdong Zhou 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1

Due to lack of optimal inductive protocols, how to effectively improve chondrogenesis of adipose-derived stem cells (ASCs) is still a great challenge. Our previous studies demonstrated that the culture media derived from chondrocyte-scaffold constructs (conditional media) contained various soluble chondrogenic factors and were effective for directing chondrogenic differentiation of bone marrow stem cells. Nevertheless, it remains unclear whether the conditional media can induce ASCs towards chondrogenic differentiation, especially for three-dimensional (3D) cartilage formation in a preshaped scaffold. In this study, it demonstrated that the conditional media derived from chondrocyte-scaffold constructs could promote ASCs to differentiate into chondrocyte-like cells, with similar expression of type II collagen to those induced by chondrogenic growth factors. Moreover, the expression level of chondrocyte-specific genes, such as SOX9, type II collagen, and COMP, was even higher in conditional medium group (CM) than that in optimized chondrogenic growth factor group (GF), indicating that the conditional media can serve as an effective inducer for chondrogenic differentiation of ASCs. Most importantly, the conditional media could also induce ASC-scaffold constructs to form 3D cartilage-like tissue with typical lacunae structures and positive expression of cartilage specific matrices, even higher contents of GAG and type II collagen were achieved in CM group compared to GF group. The current study establishes a simple, but stable, efficient, and economical method for directing 3D cartilage formation of ASCs, a strategy that may be more closely applicable for repairing cartilage defects.

Solidification of uranium mill tailings by MBS-MICP and environmental implications

Niu Qianjin,Li Chunguang,Liu Zhenzhong,Li Yongmei,Meng Shuo,He Xinqi,Liu Xinfeng,Wang Wenji,He Meijiao,Yang Xiaolei,Liu Qi,Liu Longcheng 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.10

Uranium mill tailing ponds (UMTPs) are risk source of debris flow and a critical source of environmental U and Rn pollution. The technology of microbial induced calcium carbonate precipitation (MICP) has been extensively studied on reinforcement of UMTs, while little attention has been paid to the effects of MICP on U & Rn release, especially when incorporation of metakaolin and bacillus subtilis (MBS). In this study, the reinforcement and U & Rn immobilization role of MBS -MICP solidification in different grouting cycle for uranium mill tailings (UMTs) was comprehensively investigated. The results showed that under the action of about 166.7 g/L metakaolin and ~50% bacillus subtilis, the solidification cycle of MICP was shortened by 50%, the solidified bodies became brittle, and the axial stress increased by up to 7.9%, and U immobilization rates and Rn exhalation rates decrease by 12.6% and 0.8%, respectively. Therefore, the incorporation of MBS can enhance the triaxial compressive strength and improve the immobilization capacity of U and Rn of the UMTs bodies solidified during MICP, due to the reduction of pore volume and surface area, the formation of more crystals general gypsum and gismondine, as well as the enhancing of coprecipitation and encapsulation capacity

Preparation of environmentally friendly low‐cost mullite porous Ceramics and the effect of Waste Glass Powder on structure and mechanical Properties

Wei Lian,Yan Liu,Wenjie Wang,Yangtao Dong,Sheng Wang,Zhenying Liu,Yin Liu 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.2

Environmentally friendly low-cost porous ceramics are prepared with 98.2 wt% mining waste as the raw materials at 1180 °C. The glass powder forms liquid phase at high temperatures, promoting the densification of the materials. X-ray diffraction (XRD) results show that the formation of mullite is affected by sintering temperature and content of liquid phase. The enhancement in densification is verified by the scanning electron microscopy (SEM) analysis results. According to the evolution of pore structure, the effect of the glass powder on the structure is confirmed. The number ratio of pores of < 8 μm to those of 8 ~ 20 μm is varied from 7:2 to 2:7. The minimum apparent porosity is 31.22 %, while the maximum density and linear shrinkage are 1.75 g/cm 3 and 8.48 %, respectively. The flexural strength (three-point bending method) was increased from 7.47 MPa to 28.36 MPa, indicating that the addition of glass powder could increase mechanical properties of the porous ceramics. Therefore, solid wastes can be used as the resource to develop porous mullite ceramics.

Lactobacillus casei LC01 Regulates Intestinal Epithelial Permeability through miR-144 Targeting of OCLN and ZO1

( Qiuke Hou ),( Yongquan Huang ),( Yan Wang ),( Liu Liao ),( Zhaoyang Zhu ),( Wenjie Zhang ),( Yongshang Liu ),( Peiwu Li ),( Xinlin Chen ),( Fengbin Liu ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10

Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which microRNAs and lactobacilli exert their beneficial effects on intestinal epithelial permeability. Hence, to elucidate whether miRNAs and lactobacilli play roles in intestinal epithelial barrier regulation, we compared miRNA expression levels in intestinal epithelial cells (IECs) under Lactobacillus casei (L. casei LC01) treatment. IECs and L. casei LC01 were co-cultured and then subjected to microRNA microarray assay. qRT-PCR, western blot and ELISA were used to detect the expression of occludin (OCLN) and zonula occludens 1 (ZO1/TJP1). The interaction between miRNAs and L. casei LC01 acting in IECs was investigated through transfection of RNA oligoribonucleotides and pcDNA 3.1 plasmid. The results are as follows: 1) L. casei LC01 decreased the expression of miR-144 and FD4 and promoted OCLN and ZO1 expression in IECs; 2) L. casei LC01 enhanced the barrier function of IECs via downregulation of miR-144 and upregulation of OCLN and ZO1; 3) Under L. casei LC01 treatment, OCLN and ZO1 overexpression could partially eliminate the promoting effect of miR-144 on intestinal permeability in IECs. Our results demonstrate that L. casei LC01 regulates intestinal permeability of IECs through miR-144 targeting of OCLN and ZO1. L. casei LC01 can be a possible therapeutic target for managing dysfunction of the intestinal epithelial barrier.

Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING

Wenjie Li,Huijun Guo,Yongbin Wang,Yongdun Xie,Linshu Zhao,Jiayu Gu,Shirong Zhao,Baocun Zhao,Guangjin Wang,Luxiang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.4

To identify novel allelic variations in key genes of wheat quality, the present study used the targeting induced local lesions in genomes platform to detect point mutations in target genes. The wheat variety Longfumai 17 was treated by the mutagen ethyl methanesulfonate to produce a bulk M2 generation, and the population included 1122 plants. A total length of 3906.80 kb nucleotides was analyzed, and the average mutation density was 1/244.17 kb. The identified mutations included G>A substitutions (43.75%), C>T substitutions (31.25%), A insertions (12.50%), T insertions (6.25%), and deletions (6.25%). These point mutations led to changes in amino acids and thus the encoded protein sequences, ultimately producing 18.75% of missense mutations, 12.50% of frame shift mutations, 6.25% of nonsense mutations, 25.00% of silent mutations and 37.50% of non-coding region mutations. In the kernel hardness gene Pinb and 3 starch synthesis genes waxy, Agp2 and SSIIa-A, we detected 16 different point mutations in 25 mutant lines. The Pinb gene harbored two missense mutations and a nonsense mutation; the C>T missense mutation resulted in a novel allele, this novel allele and the nonsense mutation alerted protein 3D structure; the waxy gene presented missense and frame shift mutations; the Agp2 gene carried a missense mutation; the SSIIa-A incurred a missense mutation and a frame shift mutation that resulted in premature protein termination. All the frame shift mutations, nonsense mutations and the Pinb novel allele resulted in allelic variation of their corresponding genes, which in turn affected their gene functions. The identified mutant lines can be used as intermediate materials in wheat quality improvement schemes.

An Adaptive Fault-tolerant Control Method for Robot Manipulators

Wenjie Zhang,Xiaohui Yang,Zhenghong Xu,Wei Zhang,Li Yang,Xiaoping Liu 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.12

This paper presents a new method for adaptive continuous nonsingular fast terminal sliding mode control (ACNFTSMC) based on a novel structure-improved extended state observer (SIESO) for fault-tolerant control (FTC). In response to the initial peaking phenomenon in traditional ESO (TESO), which can severely degrade the accuracy and stability of the control system, The SIESO is designed to replace TESO to estimate the lump disturbances/faults. Besides, to address the problem of the unknown estimation error of ESO, an adaptive technique is applied to compensate for the observation error in real-time. To guarantee fast convergence and chattering-free, the CNFTSMC method is employed. Afterward, the stability and rapid convergence of the control system is demonstrated using Lyapunov theory. Finally, the simulation results verify the superiority of the proposed control strategy compared to the other existing advanced control techniques.