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Bing Jia,Zhaoyang Cheng,Qi Wang,Shujun Zhang,Wei Heng,Liwu Zhu 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1
‘Dangshansuli’ pear is a yellow–green fruit when mature, whereas that of the mutant ‘Xiusu’ is russeted. In this study, thecontents of glucose, fructose and sucrose in the exocarp of ‘Xiusu’ were found to be lower than those in ‘Dangshansuli’ at75–100 days after full bloom (DAFB) but were higher at 150 DAFB. Additionally, the contents of six polysaccharides inthe exocarp of ‘Xiusu’ were higher than those in ‘Dangshansuli’ at 75 DAFB, the stage at which ‘Xiusu’ becomes russetin color, as well as at 150 DAFB; however, this was not observed for xylose. The contents of type I and II amino acids inthe exocarp of ‘Xiusu’ were higher than those in ‘Dangshansuli’ at diff erent stages, except for at 150 DAFB. The entiresequences of eight genes involved in the shikimate pathway and cell wall structure were cloned, and expression of thesegenes in the exocarp of ‘Dangshansuli’ and ‘Xiusu’ were signifi cantly diff erent during fruit development, indicating thatthese genes might be involved in the russeting process. It is speculated that the shikimate pathway connects primary andsecondary metabolism and that high contents of sugars as carbon sources provide precursors for suberin, lignin and lignanto generate russet skin in pear.
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( Qiuke Hou ),( Yongquan Huang ),( Yan Wang ),( Liu Liao ),( Zhaoyang Zhu ),( Wenjie Zhang ),( Yongshang Liu ),( Peiwu Li ),( Xinlin Chen ),( Fengbin Liu ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10
Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which microRNAs and lactobacilli exert their beneficial effects on intestinal epithelial permeability. Hence, to elucidate whether miRNAs and lactobacilli play roles in intestinal epithelial barrier regulation, we compared miRNA expression levels in intestinal epithelial cells (IECs) under Lactobacillus casei (L. casei LC01) treatment. IECs and L. casei LC01 were co-cultured and then subjected to microRNA microarray assay. qRT-PCR, western blot and ELISA were used to detect the expression of occludin (OCLN) and zonula occludens 1 (ZO1/TJP1). The interaction between miRNAs and L. casei LC01 acting in IECs was investigated through transfection of RNA oligoribonucleotides and pcDNA 3.1 plasmid. The results are as follows: 1) L. casei LC01 decreased the expression of miR-144 and FD4 and promoted OCLN and ZO1 expression in IECs; 2) L. casei LC01 enhanced the barrier function of IECs via downregulation of miR-144 and upregulation of OCLN and ZO1; 3) Under L. casei LC01 treatment, OCLN and ZO1 overexpression could partially eliminate the promoting effect of miR-144 on intestinal permeability in IECs. Our results demonstrate that L. casei LC01 regulates intestinal permeability of IECs through miR-144 targeting of OCLN and ZO1. L. casei LC01 can be a possible therapeutic target for managing dysfunction of the intestinal epithelial barrier.
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