韓國 傳統紙花에 관한 硏究 (14)

김태연(Kim, Tae-yeon) 한국조형디자인학회 2008 조형디자인연구 Vol.11 No.4

After being born, all humans shall face death and they wonder about death. Moreover, they doubt and afraid of the world after death. Therefore, they have generated faith and religion in order to reply on and believe in something. Traditionally, we have called it pray, pray blessing, Bison(rubbing hands prayerfully), worship, rite offered before a statue of Buddha, memorial service and exorcism. If we call it ceremony in general, the order of practice concerned to who, when, where, what and how can be the basic principle of all religious ceremonies. Moreover, in all religious phenomenons, ceremony is embodied as a song or dance and the ceremony space is embodied as a molding and color. It means the Jangeom shown in Buddhist ceremony and it can be the historical origin of appearance of chrysanthemum. The person, who was dead from the accident in land, water or air, or the person, who was unnaturally dead through suicide, is called a lonely spirit. Surukjae is the Buddhist ceremony to invite spirit of the person, confute dharma and serve delicious food. The Surukjae has a direct relationship with a formative background of Gamrowangdo(painting). Gamrowangdo is a Buddhist picture for ceremony that leads a spirit of dead person. In the picture, Buddhist world view is synthetically collected. In the bottom of picture, the six worlds attracting people's attentions are displayed. It shows various images from Cheoningwonsok to lonely spirit and minute life form that face unexpected death in various karmas. The lonely spirit on the bottom escapes from the pain of karma and gets the mercy of Buddha on the top by receiving the ceremony of Buddhist priests and tasting consecrated food. At this time, Inrowangbosal plays the role of mediator that leads all sentient beings of six worlds to pure west land of Buddha. Like that, Gamrowangdo shows dramatic composition of organic relation between the top, middle and bottom in one picture. Among the flowers shown in Gamrowangdo, the author grasped the features of flowers through the method to select and analyze 10 pictures including Gamrowangdo of Ssanggyesa(temple). Therefore, Jangeom is the shape of effort symbolizing the behavior of Buddha. In Buddhism, special religious meaning is granted according to the word, 「belief is riginated from Jangeom」. As shown in the flower picture expressed in Gamrowangdo, paper flower becomes the important factor of Jangeop and in Buddhist seminary, it is expressed that truth of Buddha is scattered and embroidered like flowers. Kim Tae-suk used thin flower paper or that painted by watercolor. Its characteristic is clean and fine. Because the leaf is dyed with weak pigment, its color is relatively clean and fine. The kind of paper flower is peony, mum, Ddri flower, lotus and dicentra. The desire of researcher is to find, preserve and inherit traditional Jangeop technique for Buddhist ceremony. The researcher performed the study with a sense of duty to have self-esteem as a traditional craftsman, establish the place for lifelong education to teach the technique and deliver Korean Buddhism culture through museum and exhibition hall. Moreover, the researcher wants to inspire the importance of cultural value of unique Korean Buddhism.

Melt spinning 법으로 제조된 Ti-Ni-Cu 합금리본의 상변태거동 및 형상기억특성

金載勳,崔美善,李喜佑,金泰俊,김연욱,南泰鉉 대한금속재료학회 2002 대한금속·재료학회지 Vol.40 No.7

Phase transformation behavior and shape memory characteristics of Ti-Ni-Cu alloy ribbons fabricated by melt spinning have been investigated by means of differential scanning calorimetry, X-ray diffraction, optical microscopy and thermal cycling tests under constant load. With increasing linear velocity and decreasing ejection pressure, transformation start temperatures of ribbons decreased and stability of the B19 martensite was lowered, comparing to those of alloys fabricated by conventional casting since the cooling rate increased. Only after heat treating ribbons at temperatures higher than 873 K where recrystallization occurred, the B19 martensite was observed in Ti-40Ni-10Cu alloy ribbons. Transformation elongation and hysteresis associated with the B2-B19 transformation in Ti-Ni-Cu alloy ribbons decreased with increasing Cu-content, which was attributed to the decrease in lattice deformations. Transformation elongations of ribbons were larger than those of alloys made by casting, which was closely related to a texture developed in ribbons.

당뇨병성 합병증을 가진 환자에서 혈중 Erythropoietin 농도

김동규,유기동,허광식,김상용,윤성호,조영신,권용은,김태원,김건영,정종훈,배학연 朝鮮大學校 附設 醫學硏究所 1998 The Medical Journal of Chosun University Vol.23 No.1

연구 배경 : 고혈당성에 의한 산화환원반응 이상(가저산소증)이 조절 되지않는 당뇨병의 특징으로 혈관과 신경 기능에 대한 진성 저산소증의 효과와 유사하며, 당뇨 합병증의 병태생리에 중요한 역할을 한다. 고혈당이 있는 인슐린 비의존형 당뇨병 환자에서 인슐린 수준이 정상이듯이, 빈혈이 있는 당뇨병 환자에서 EPO의 농도는 실제 혈색소 농도의 감소비율과 차이가 있을 것이라 추측된다. Friedman 등은 당뇨병성 합병증 원인 인자로 가저산소증(pseudohypoxia) 또는 저산소증(hypoxia)을 제기하였고 이런 인자들이 EPO의 상대적 또는 절대적 결핍에 의한 것임을 보고하였다. 방법 : EPO-Trac^(TM 125)I RIA kit을 이용하여 방사면역측정법으로 EPO 수준을 검사하였다. 전혈 3㎖을 5-10㎖ 시험관에 정맥 채혈하였으며, 용혈과 장기간의 보존을 위하여 원심분리를 즉시 시행하여 혈청을 영하 200C에서 냉동 보관 후 일괄적으로 검사 결과를 얻었다. 결과 : 1996년 9월부터 1997년 2월까지 조선대학교 부속병원 내과에 입원한 2형 당뇨병 환자 63례를 대상으로 하여 다음과 같은 결과를 얻었다. 1) 당뇨병성 합병증이 없는 군과 있는 군간의 혈색소, 혈중 EPO농도의 차이는 유의한 차이가 있었으며 혈색소의 감소율보다 혈중 EPO의 감소율이 더 높았다. 2) 당뇨병성 망막증의 유무에 따른 혈색소 농도의 차이는 유의한 차이가 없었으나 혈중 EPO농도는 유의한 차이가 있었다. 증식성군에서만 혈중 EPO의 감소비율이 혈색소에 비해 높았다. 3) 당뇨병성 신증의 유무에 따른 혈색소, 혈중 EPO농도는 유의한 차이가 있었고 혈색소 감소율에 비해 EPO농도의 감소율이 높았다. 신증의 중증도에 따른 혈색소, EPO의 차이는 미세알부민뇨군을 제외하고는 유의한 차이를 보였고 혈색소 감소율에 비해 EPO의 감소율이 더높았다. 4) 당뇨병성 신경병증의 유무에 따른 혈색소 농도의 차이는 유의한 차이가 없었으며 EPO농도는 유의한 차이를 보였다. 혈색소와 EPO의 감소비율은 비슷하였다. 신경병증의 중등도에 따른 혈색소와 EPO농도의 변화는 유의한 차이가 없었으나 stage 3에서는 혈색소감소율보다 EPO감소율이 더높았다. 결론 : 당뇨병성 합병증을 가진 환자에서 빈혈의 정도는 대부분 혈청 EPO치의 절대적 감소에 의함을 간접적으로 밝혀낼 수 있었으며 차후 더 많은 대상으로 비교 분석이 필요하리라 사료된다. Background: Hyperglycemic-induced redox(pseudohypoxia) imbalance is a characteristic feature of poorly controlled diabetes that mimics the effects of true hypoxia on vascular and neural functions and plays an important role on the pathogenesis of diabetic complications. As is true for apparently "normal" insulin levels typically found in NIDDM even in the presence of hyperglycemia, a "normal" erythropoietin level in an anemic diabetic subject may be disproportionally low for the actual red cell mass. Therefore, Friedman et al suggested that pseudohypoxia or hypoxia as an etiological factor of diabetic complications are due to absolute or relative erythropoietin deficiency Method: EPO-TracTM 125I RIA kit was used for the quantitative determination of erythropoietin(EPO) in serum by radioimmunoassay. An adequate sample of blood (3ml whole blood) was collected aseptically by venipuncture in a 5~10ml glass tube to yield a minimum of 400 L of serum per assay. The serum was promptly removed from the clot by centrifugation in order to avoid hemolysis. Then to increase its storage time it was frozen at -200C in a nonself defrosting freezer. Finally, tests were undertaken simultaneously Results We studied 63 cases with diabetes mellitus, who were admitted to Chosun University Hospital from September, 1996 to February, 1997 at the Department of Internal Medicine. We defined the control group, as diabetic patients who did not have anemia(<13mg/dl), diabetic complications(retinopathy, nephropathy, neuropathy) and the remainders were defined as the experimental group(we excluded anemic patients, who had secondary causes of anemia and diabetic patients with end stage renal disease)Data were as follow 1) The relationship of Hb and the 24hr urine protein between diabetic patients with and without complications significantly differed(p=0.02, < 0.001 respectively), but the Hb level was poorly related between diabetic patients with and without retinopathy(except in preproliferative, proliferative subgroups) and neuropathy. 2) Subgroups of patients with diabetic complications had higher 24hr urine protein than patients without diabetic complications, except stage I diabetic neuropathy 3) The EPO level was significantly different between diabetic patients with and without complications. 4) The correlation between EPO and Hb was significantly different, especially in diabetic patients with retinopathy and nephropathy according to severity of diabetic complications, compared with patients who did not have diabetic complications such as retinopathy and nephropathy. Conclusion: We know that anemia induced by diabetic complications is due to relative EPO deficiency than absolute EPO deficiency, and further evaluation and studies are needed on many cases in the future

중학교 가정과 '인간발달과 가족관계' 영역의 교육자료 (CD-Rom) 개발 및 현장 적용 연구 : 실천적 추론 수업을 중심으로

유태명,장혜경,유지연,김주연,김항아,김효순 한국 가정과 교육 학회 2000 한국가정과교육학회지 Vol.12 No.3

The purpose of this study is to develop lesson plans. teaching guide, and teaching materials applying practical reasoning teaching model in the area of 'human development and family relationship' in middle school home economics. The practical reasoning teaching model consists of five factors: Desired results, Awareness of context. Alternative approaches, Consequences of action, and Action. This study based on practical reasoning has following process: Curriculum development, Developing lesson plan. teaching material, and teaching guide. Experimental teaching and evaluation, Feedback, Production of CD-Rom. Teaching guide includes lesson Plan. workbook, multimedia materials and teaching resources, Especially teaching guide in CD-Rom can be used effectively in the actual teaching. In the classroom, this teaching model accomplished active and interesting Participation of teachers and students. It is proposed that practical reasoning teaching model should be applied to other areas of home economics. In addition, various teaching materials based on Practical reasoning need to be developed.

불교의례의 지화장엄―장인별 지화(紙花)기법의 특징을 중심으로―

김태연 ( Kim Tae-yeon ) 한국디자인트렌드학회 2008 한국디자인포럼 Vol.21 No.-

본 연구는 1981년부터 발표하고 있는 한국 전통지화(傳統紙花)에 관한 연구 (1)~(14)의 내용 중 불교의례를 중심으로 연구된 논문을 바탕으로 영산재(靈山齋) 및 수륙재(水陸齋)의 지화장인을 중심으로 지화기법의 특성에 대하여 비교 발표한다. 그 동안의 연구에 의하면 중요 무형 문화재 제50호로 지정 된 영산재의 장엄 중 지화 제작 기법의 기능을 보유하고 있는 지화장인은 黃銀同(月華스님), 李基遠 처사, 李秉祐(慶庵스님), 尹鍾規(海月스님), 李周奐(石龍스님)으로 조사 되었다. 한편 수륙재의 장엄 중 지화 제작의 기능을 보유하고 있는 지화장인은 金泰淑이가 있다. 이들 장인별 지화 제작에 대한 특성을 중심으로 비교조사해 본 결과는 다음과 같다. 영산재의 지화장인인 黃銀同(月華스님)의 장엄지화의 종류는 운화를 비롯하여 12종류이며, 李基遠의 장엄지화의 종류는 살모란을 비롯하여 모두 7종류이다. 또한 李秉祐(慶庵스님)의 장엄지화의 종류는 싸리꽃을 비롯하여 5종류이며, 尹鍾規(海月스님)의 장엄지화종류는 수국을 비롯하여 6종류이다. 그리고, 李周奐(石龍스님)의 장엄지화의 종류는 대국화를 비롯하여, 모두 9종류로 조사 되었다. 한편 수륙재 지화장인인 金泰淑의 장엄지화·종류는 작약을 비롯하여 모두 6종류인데 최근 불가에서는 수륙재에 관심을 갖고 오랜만에 실행하고 있는 사찰도 더러 있다. 이와 같은 불교의례의 장엄지화기법의 종류는 모두 45가지로 조사 되었다. 이 중에 서로 명칭은 같은 것도 있으나 ,장인마다의 솜씨에 따라 기법은 다양하게 표현 되어 똑 같은 모양의 지화는 거의 없었다, 따라서 본 연구는 점차 간소화되어 사라져 가고 있는 불교의례의 原形을 보존하는데 기여하고자 한다. 그리하여 영산재와 수륙재의 장엄지화의 전통기법을 보존하고 지화 장인들의 기능보유를 위한 문화 정책적 지원을 강조하면서 연구의 의의를 두고자 하였다. This study was aimed at comparing and analyzing the techniques and craftsmanship in the making of the decorative paper flowers for the two typical Buddhist Rituals, Su-ryuk-jae and Yeong-san-jae, the latter designated as the Important Intangible Cultural Property NO. 50. The paper flowers used in the Buddhist rites surpass any other kind in size and exquisiteness, thus acquiring the name of `jang-eum` or solemn decorations. The present study was based on a series of 14 studies on the traditional paper flowers in Korea presented by the author since 1981, but in this paper more emphasis was placed on the differences not only between the two rites but also in the craftsmanship and paper materials depending on the individual craftsman. There exist five prominent skill retainers for the Yeong-san-jae paper flowers: Hwang Eun-dong (Monk Wolhwa), Recluse Lee Gi-won, Lee Byeong-woo (Monk Kyeongam), Yun Jong-gyu (Monk Haewol) and Lee Ju-hwan (Monk Seokyong), where as Kim Tae-suk is the only technique retainer for Su-ryuk-jae. The results of the study are as follows: Hwang Eun-dong (Monk Wolhwa), makes twelve kinds including cloud flowers; Recluse Lee Gi-won seven including peonies; Lee Byeong-woo (Monk Kyeongam) has techniques for five kinds, including bush clovers; Yun Jong-gyu (Monk Haewol) holds skills for six kinds. Lee Ju-hwan (Monk Seokyong) retains techniques for making nine kinds of flowers besides big chrysanthemums. Kim Tae-suk specializing in the Su-ryuk-jae paper flowers has the expertise of making six different kinds of decorative flowers along with peonies. In conclusion, a total 45 different kinds of paper flowers are presently being produced by these representative artists. The techniques employed by the individual craftsman often share the same or similar names, but as the workmanship and techniques of the artists vary widely, their works denote these differences, thus strongly requiring us to restore the originality of the authentic forms and quality of paper flowers while stimulating the artists` creativity ever more.

21가지 장미꽃 품종의 페놀성 화합물 함량 및 항산화 활성

조연재(Yeon Jae Jo),서정현(Jeong Hyun Seo),홍채영(Chae Young Hong),김성태(Seung Tae Kim),최은경(Ehn-Kyoung Choi),김윤배(Yun-Bae Kim),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.4

본 연구는 21품종의 장미꽃에 대한 식품 및 화장품 원료로 사용 가능성을 살펴보기 위해 페놀성 화합물과 항산화 활성을 평가하였다. 총 폴리페놀, 총 플라보노이드 및 타닌 함량은 러버샤이 품종에서 각각 379.70 mg GAE/extract g, 50.10 mg CE/extract g 및 339.44 mg TAE/extract g으로 높게 나타났으며, 칠백리 품종에서 각각 107.25 mg GAE/extract g, 9.46 mg CE/extract g 및 36.31 mg TAE/extract g으로 낮았다. 페놀산 함량은 총 폴리페놀 함량과 유사한 결과를 보였으며, 러버샤이 품종에서 27.40 mg/extract g으로 높았다. 안토시아닌은 대부분 cyanidin-3,5-diglucoside로 확인되었으며 총 안토시아닌 함량은 유니나 품종에서 8.80 mg/extract g으로 가장 높았다. ABTS 및 DPPH 라디칼 소거 활성은 각각 428.10 및 419.59 mg AAE/extract g로 러버샤이 품종에서 가장 높았으며, 항산화 활성과 페놀 화합물의 함량과 높은 양의 상관관계를 보였다. 이러한 결과는 러버샤이와 같은 항산화 활성이 뛰어난 품종들이 관상 목적뿐만 아니라 높은 기능성을 가진 식품 및 화장품의 원료로 사용 가능성이 있다고 판단된다. This study evaluated the functional components and antioxidant activities of 21 different rose flower extracts, to confirm their value as a food and cosmetics ingredient. The highest values of total polyphenol, flavonoid and tannin contents (379.70±1.64 mg GAE/extract g, 50.10±0.76 mg CE/extract g, and 339.44±1.20 mg TAE/extract g, respectively) were obtained in Lover Shy. The proanthocyanidin content ranged from 61.88 mg CE/extract g in the Unina cultivar to 5.34±0.13 mg CE/extract g in the Aileen cultivar. The phenolic acid content obtained was highest in the Lover Shy cultivar (27.40±0.26 mg/extract g), whereas highest levels of anthocyanin (8.83±0.03 mg/extract g) were obtained in the Unina cultivar. High ABTS and DPPH radical scavenging activities were determined in the Lover Shy cultivar (428.10±0.29 and 419.59±3.12 mg AAE/extract g, respectively). Taken together, these results indicate that the antioxidant activity and functional components of Lover Shy cultivar are superior, and this extract can potentially be applied as a food and cosmetic ingredient.

Original Article : Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

( Ji Yeon Son ),( Yoon Jong Kang ),( Kyeong Seok Kim ),( Tae Hyung Kim ),( Sung Kwang Lim ),( Hyun Jung Lim ),( Tae Cheon Jeong ),( Dal Woong Choi ),( Kyu Hyuck Chung ),( Byung Mu Lee ),( Hyung Sik Ki 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

실험적 급성 심근경색증 후 좌심실 재성형에 관여하는 간질 조직의 변화에 대한 전환효소억제제의 기전에 대한 연구 : 백서의 非痛璧性 심근경색 후 좌심실 재성형 및 Transforming Grawth Factor β-1의 발현 변화와 이에 대한 레닌 안지오텐신계 차단제의 효과 분석 Effects on Transforming Growth Factor - β-1 Expression

연태진,김석연,김효수,김어진,김소영,정은주,서정욱,오병희 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Ji Yeon Son,Yoon Jong Kang,Kyeong Seok Kim,Tae Hyung Kim,Sung Kwang Lim,Hyun Jung Lim,Tae Cheon Jeong,Dal Woong Choi,Kyu Hyuck Chung,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.