플라스틱 V-벨트 풀리 설계 및 시작품 제작에 관한 연구

손태일,임재규,김형종 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

In this study, product design and prototype manufacturing of a plastic water-pump pulley has been tried. The designed model is supposed to be made of 33% glass reinforced resin of which the tensile strength is 180 MPa, and has 24 ribs on each side to increase its structural strength. Structural analysis under a static load of 300kgf acting on both edges of the belt has been carried out using a commercial finite element code, MARC. The analysis result showed the maximum effective stress near a rib of designed model would be at must 35MPa (less than 20% of the tensile strength), therefore, the plastic product would be sufficiently safe under that loading condition. On the basis of the structural analysis, a prototype of the designed model has been manufactured by using the fused deposition modelling (FDM) method which is one of the rapid prototyping (RP) methods, using ABS resin and support materials. The CAD data exported to the RP system in STL format was prepared by a commercial solid modeling soft ware, SolidWorks. It has been proved that the plastic pulley can successfully replace the existing flow-farmed steel product.

완두 세포질 Fructose-1,6-Bisphosphatase cDNA의 PCR Cloning과 특성

손태종,한태룡 경희대학교 유전공학연구소 1996 遺傳工學論文集 Vol.8 No.-

Three positive phage clones were isolated from cDNA phage clones which had been first screened from a pea cDNA library with polyclonal antibodies for the purified pea cytosolic fructose-1 6-bisphosphatase (FBPase). Polymerase chain reaction was conducted with these phage clones using two primers synthesized from homology analysis of amino acid sequences for animal and plant cytosolic FBPases. A PCR product with 650 bp long was cloned into pGEM-T vector and sequenced The deduced amino acid sequence of the cDNA fragment was 98, 91, and 85% homologous with those of cytosolic FBPases from spinach, sugarbeet, and sugarcane, respectively. It was 86 and 51% homologous with amino acid sequences of FBPase from rat liver and pea chloroplasts, respectively. Northern blot analysis was proceeded with the cDNA clone resulting that 1.2 kb transcript was highly expressed in light grown pea leaves but almost not expressed in dark grown etiolated pea seedlings. Peas grown in the light for 10 days were transferred to darkness, showing that the transcript was gradually decreased during dark treatment, indicating that the expression of the enzyme was induced by continuous white light but suppressed by dark treatment. Pea cytosolic FBPase was highly expressed in leaves with trace amounts in stems, but almost not expressed in roots.

外傷性 肝臟破裂과 肝膽道出血患者의 肝動脈結柴에 依한 治療

孫鍾夏,咸泰元,林敏一,裵振善,韓萬靑 대한적십자사 1974 적십자병원지 Vol.1 No.1

가열처리에 의한 단감의 이화학적 특성

손규목,김광호,성태수,김종현,신동주,정지영,배영일 한국식품영양학회 2002 韓國食品營養學會誌 Vol.15 No.2

단감의 기능성 소재 및 기호성이 우수한 부원료로서의 이용 가능성을 확인하기 위하여 단감새옥를 전처리별 즉, 열처리 온도 (25℃, 75℃ 및 95℃) 에서 NaCl (0, 1, 3%) 농도별로 침적 (1, 5분) 하여 동결건조한 시료구를 대상으로 탄닌, 비타민 C, 색도 및 조직감 등을 분석한 결과는 다음과 같다. 탄닌 성분은 열처리 온도, NaCl 농도 및 침적시간이 증가할수록 탄닌함량이 감소하는 경향을 나타내었으며, 특히, 대조구 (420 mg%) 에 비해 95℃에서 1% 및 3% NaCl 농도에 5분간 침적시 각각 228 및 198 mg%로 감소하였다. 비타민 C 함량은 대조구 (122.4 mg%)에 비해 95℃에서 3% NaCl 농도에 5분간 침적시 75.8 mg% 로 감소하였고, 색도는 열처리 온도, NaCl 농도 및 침적시간이 증가할수록 L(명도) 및 b(황색)값이 뚜렷하게 증가한 반면, a(적색도) 값은 감소하였고, ??E 값은 증가하여 처리조건이 가중될수록 색의 변화가 진행됨을 알 수 있었다. 단감껍질과 전처리한 시료 (95℃, 1% NaCl, 5 분)의 flavonol 함량은 껍질에 myricetin(2.0 ㎍/g), quercetin (34. 5 ㎍/g) 및 kaemferol (1.1 ㎍/g) 성분이 검출되었고, 전처리한 시료는 myricetin (9.5 ㎍/g), quercetin (5.5 ㎍/g)이 검출되었다. 조직감은 대조구에 비해 전처리 (95℃, 1% NaCl, 5분) 한 시료에서 부서짐성, 응집성, 점성 및 씹힘성이 우수하였다. 관능검사는 95℃에서 1% NaCl에 5분 및 3% NaCl 농도에 1부간 침지한 시료가 좋은 평가를 받았다. Sweet persimmon were tested in order to identify their use as secondary material which is excellent in function and taste as food. Samples were soaked for 1 and 5 min with NaCl concentration (0, 1 and 3%) at a certain heating temperature (25, 75 and 9r℃), and then tannin, vitamin C, flavonol, color intensity, sensory test and textural properties were analyzed. The results of the analyses were as follows. Tannins were decreased as heating temperature, NaCl concentration and soaking time were increased, especially, that the control was 420 mg% but decreased 228 and 198 mg% at 95℃ (1 and 3% NaCl concentration) for 5 min, soaked in each. Vitamin C content also decreased more in higher temperature and NaCl concentration than control (122.4 mg%). Color intensity showed higher value in L and b than in heating temperature, NaCl concentration and soaked time longer remarkably, but a value decreased. The peel of sweet persimmons was analyzed myricetin (2.0 ㎍/g), quercetin (34.5 ㎍/g) and kaemperaol (1.1 ㎍/g), but in pre-treatment sample ( 95℃, 1% NaCl concentration and 5 min, soaked ) was showed higher myricetin (9.5 ㎍/g) and quercetin (5.5 ㎍/g). Textural properties were good in pre-treatment sample ( 95℃, 1% NaCl concentration and 5 min, soaked) such as brittleness, cohesiveness, gumminess and chewiness. In sensory analysis, the pre-treatment samples (95℃, 1% NaCl concentration and 5 min, soaked and 95℃, 3% NaCl concentration and 1 min, soaked) were showed higher point than others.

대장암 환자에서의 담즙 암태아성 항원(CEA)

김종우,박용태,정철운,권성원,장항석,손석우,안대호,이경포 대한대장항문학회 2000 Annals of Coloproctolgy Vol.16 No.6

농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례

박태혁,김정일,손지언,김종국,김형수,정갑열,김준연 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.4

목적 : 농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례 보고. 방법 : 피로감, 전신무력감, 어지럼증, 보행장애 등의 증상을 주소로 내원한 브롬화메틸을 사용하여 방역작업에 종사하는 건강한 20세와 18세의 근로자를 대상으로 혈액검사, 신경정도 및 근전도검사, 뇌자기공명영상 등의 임상검사와 작업력조사를 시행하였다. 결과 : 1례에서 뇌자기공명영상에서 연수와 소뇌의 양측 측충부의 고신호강도가 관찰되었고 11일 후의 추적조사에서 동일 부위의 고신호강토가 감소된 소견이 관찰되었으며 뇌병증으로 최종 진단되었다. 다른 1례는 신경전도검사에서 다발성 신경병 소견과 뇌유발전위검사에서 우측 하부 뇌간부위 병소가 관찰되었고 뇌병증 및 말초신경 병으로 최종 진단되었다. 결론 : 근로자들의 증상은 소독과정에서 브롬화메틸 노출로 인한 신경 병으로 판단되며 훈증소독과방역작업에 종사하는 근로자들의 실태조사와 안전에 대한 대책이 시급하다고 하겠다. Objectives : To report two cases of neuropathy due to methyl bromide intoxication. Methods : Workers, engaged in the fumigating process, complained fatigue, general weakness, ataxia, and hypersomnia. We evaluated them with blood tests, neurophysiologic studies and MRI and investigated their occupational history. Results : Increased signal intensities were found in the medulla oblongata and paraver mian of cerebellum in MRI and after 11 days, high signal intensities were reduced in the following MRI. In the other case, polyneuropathy and rlght lower brainstem lesion were observed In the NCV and BAEP studies. Conclusions : We confirmed that worker's symptoms were related to methyl bromide exposure in the fumigation. It is necessary that we should evaluate present condition of fumigating process and prepare appropriate methods to protect workers engaged in the fumigation.

토양에서 분리한 Toluene 분해 세균의 동정 및 일부 특성

김종구,박근태,이건,이상준,손홍주 부산대학교 환경문제연구소 1998 環境硏究報 Vol.16 No.-

경상남도 양산천 일대의 토양시료로 부터 toluene을 유일한 탄소원 및 에너지원으로 생육하는 미생물들을 분리하였고, 그 중 toluene의 분해능이 가장 우수한 균주를 선별하여 공시균으로 선택하였다. 공시균의 형태학적, 배양적, 생화학적 및 화학분류학적 제반 특성들을 토대로 분류학적 위치를 검토한 결과 Rhodococcus 속으로 동정되어 편의상 Rhodococcus sp. EL-43P로 명명하였다. Rhodococcus sp. EL-43P는 phenol 및 다양한 phenol 유도체, benzene 등의 방향족화합물 기질에서 생육할 수 있었다. 특히 phenol 분해에 관련된 각종 환경조건을 최적화시키면 실제의 phenol 함유 폐수처리공정에서 충분히 적용가능성이 있음을 알 수 있었다. Rhodococcus sp. EL-43P는 catechol 1,2-dioxygenase의 활성을 가지고 있어 ortho pathway에 의하여 phenol을 분해함을 알 수 있었다. 또한 본 공시균은 toluene dioxygenase의 활성도 가지고 있었다. The microorganisms which degrade toluene as the sole and energy source were isolated from soil around Yangsan river. Among them, the strain EL-43P had the highest toluene degradability and identified as a Rhodococcus sp. according to morphological, cultural, biochemical and chemotaxonomical properties. Rhodococcus sp. EL-43P was able to grow at the various chlorinated phenols such as o-, p-chlorophenol, 2,3-,2,5-, 2,6- and 3,4-dichlorophenol, and benzene, respectively. If detailed phenol degradation conditions are established, the treatment of phenol wastewater using Rhodococcus sp. EL-43P would be possible. Rhodococcus sp. EL-43P had the catechol 1,2-dioxygenase which degrades Phenol derivatives by ortho pathway. This strain also had toluene dioxygenase.

급속응고된 N-type Bi₂Te_(2.75)Se_(0.15) 열전재료의 미세조직과 열전특성에 미치는 압출비의 영향

김태경,이상일,임종호,손현택,김택수,천병선 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.1

The n-Type thermoelectric compounds of Bi₂Te_(2.75)Se_(0.15) doped with 0.1 wt% SbI₃ were fabricated by gas atomization process and extruded under ratio of 16:1 and 28:1 at 450℃. The effect of extrusion ratio on the microstructures and thermoelectric properties were investigated by a combination of microscopy, XRD and thermoelectric properties. Grains of extruded bars are smaller than those of heated powder at extrusion temperature (450℃) due to the dynamic recrystallization but with increasing the amount of plastic deformation, grains of specimen extruded with 28:1 were slightly coarse. The compressive strength of hot extruded bar under 28:1 is 160 MPa and with decreasing the extrusion ratio to 16:1, the value is 250 MPa. The Seebeck coefficient a and electrical resistivity p were decreased with increasing ratio, while thermal conductivity x was increased. this results from decrease of carrier scattering and increase of carrier mobility. Extruded bar under ratio of 16:1 shows the higher value of figure of merit (Z=2.50x10^(-3)/K) than that of 28:1 (Z=2.07x10^(-3)/K). (Received June 24, 2004)

사람 태반조직 DNA Topoisomerase Ⅰ에 대한 Phosphorylation의 영향

곽상태,김승민,박종일,손미영,임규,황병두 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

DNA topoisomerase I was prepared from human term placenta and the effects of phosphorylation/dephosphorylation to the enzyme activity have been investigated. hosphorylation of the enzyme by protein kinase'A or casein kinase II did not affect the activity of DNA topoisomerase I. But dephosphorylation of the enzyme by calf intestinal alkaline phosphatase decreased the DNA topoisomerase I activity. The inhibitory effects of camptothecin or 10-OH-camptothecin on the enzyme were not related to dephosphorylation of the enzyme. These results suggest that DNA topoisomerase I from human term placenta present in forms of phosphorylated, and regulation of the enzyme activity is related with phosphorylation/dephosphorylation of the enzyme.

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.