XPS와 AFM에 의한 올리고펩타이드의 자기 집합화 단분자막 분석

송성훈,박진영,김우식,Nakamura, Chikashi,Miyake, Jun,장상목 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.1

금속 기판 위에 유기 박막을 형성하는 방법 중에서 티올기와 Au와의 결합을 이용한 자기집합에 관한 연구가 활발히 진행되고 있다. 포르피린 유도체와 강하게 결합하는 타올기를 가지는 올리고펩타이드 PSP1과 PSP2를 합성하여 MOPS 완층용액에서 금표면에 자기집합화 단분자막(self-assembled monolayer, SAM)을 형성시켜 X-ray photoelection spectroscopy(XPS)와 atomic force microscopy(AFM)을 이용하여 분석하였다. 그 결과 PSP2의 경우와 PSP1의 경우보다 Au표면에서 SAM이 더욱 더 잘 형성되고 있음을 알 수 있었고 MOPS완충용액에 존재하는 황 이온의 영향도 알 수 있었다. 이 결과는 구성단위로부터 이론적으로 계산한 SAM막의 steric energy 값의 차이에서 유추한 결론과 일치하였다. Recently, the self-assembled monolayer(SAM) of thiols on nible metals has been studied intensively. Oligopeptides such as PSP1(Tyr-Ala-Gly-Tyr-Cys) and PSP2(His-Ala-Ser-Cys) with thiol group, which interact strongly with a typical cationic porphyrin derivative, are synthesized. Peptides adsorption from the liquid phase on Au has been studied using X-ray photoelection spectroscopy(XPS) and AFM image. XPS provides further evidence that the primary adsorbate species is bonded to Au through the sulfur atom. By the analysis of XPS spectra and AFM image, it is found that PSP2 is self-assembled better than PSP1 and the sulfur ion MOPS buffer solution has an effect on the self-assembling performance. This result is coincident with the calculation result of steric energies of Au-Peptide bonded SAM structure.

American Medical Association 5판을 근거로 한 한국형 말초신경계 장애 평가기준 개발

박동식,김병조,황선철,송인욱,손병철,장성호,정두신 순천향대학교 의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.14 No.3

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개에 있어서 심장사상충증을 동반한 창상성 횡격막 허니아의 수술적 치료 1례

조승혁,임영환,손정민,노경완,박창식,전무형,김명철,송근호,정성목 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

A 7-month old. castrated male Shih-tzu dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with history of depression. dyspnea. mild scale. tachypnea. exercise intolerance and severe leanness. On physical examination. cardiac arrhythmias was heard and heart worm ELISA test was positive. Microfilaria was found on the direct blood smear. Survey radiographs showed loss of diaphragmatic line and cardiac silhouette. displacement of lung fields and presence of gas filled intestines in the thoracic cavity. Rib fracture and pelvic fracture with callus formation were found incidentally. On the basis of physical examination and radiographic findings. it was diagnosed as traumatic diaphragmatic hernia and heart worm disease. Herniorrhaphy was performed. Postoperatively. it was treated for heart worm disease and scale. There is no evidence of recurrence of diaphragmatic hernia and heart worm disease over 1 year.

구개측으로 매복된 상악견치의 교정치료기간에 영향을 미치는 요소

정송화,김성식,조봉혜 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.2

구개측으로 매복된 견치와 인접구조물 간의 위치관계가 전체적인 교정치료기간에 어떤 영향을 미치는지 알아보고자 편측성 구개측 견치 매복으로 진단받고, 교정적인 견인을 하여 치료를 완료한 환자 36명의 초진 시 파노라마 방사선사진을 대상으로 연구하였다. 전체 치료기간의 평균인 21개월을 기준으로 나누었을 때, 장기치료군에 비해 단기치료군에서 인접 측절치의 치근에 견치의 치관이 겹치는 정도가 더 크게 나타났다. 치료기간을 예측하기 위한 중회귀분석에서 상악견치의 치관에서 치조정까지의 교합면에 대한 수직거리를 나타내는 HCV (Height of the canine crown vertically)와 하악견치간폭경이 각각 0.142와 0.115의 예측상수(r2)를 보였으며, 두 인자를 같이 고려하였을 경우에는 0.208의 예측상수를 보였다. 나이에 따른 분류에서 치료기간, 상악 견치 치관에서 치조정까지의 수직거리 (HCV), 중절치 정중선에 대한 견치의 각도가 초기 영구치열기까지는 점진적으로 감소를 하다가 완성 영구치열기에서 증가하였다. 본 연구의 결과, 구개측으로 매복된 견치의 견인을 시행할 때에는 초기 영구치열기에 견치의 치관첨이 교합면에 가깝고 직립이 되어 있을 경우에 전체 교정치료 기간이 짧아진다는 것을 알 수 있었다. Objective: The aim of this study was to determine the relationship between the status of a unilateral palatally impacted maxillary canine (as seen on the panoramic radiograph) and the orthodontic treatment duration. Methods: A total of 36 subjects were chosen (8 males and 28 females, 13.7 ± 2.5 years). All patients had undergone orthodontic traction of the impacted canine after a closed flap surgery. The position of impacted canine on the panoramic radiograph was traced and calculated with regard to the treatment duration. Results: The canine overlap over the lateral incisor (COGr) had significant statistical difference between the short-term and the long-term treatment duration groups (divided by average treatment time of 21 months) (p < 0.05). Multiple regression indicated that the variables of significance (with treatment duration as the dependent variable) were the canine vertical height from the occlusal plane (HCV, r2 = 0.115; p < 0.05) and the mandibular anterior width (MnDW, r2 = 0.142; p < 0.05). The treatment duration, the canine angle to the incisor midline (CA), and HCV decreased from primary dentition (under 12 yrs) to permanent dentition (15 yrs). Howerer, these increased again at the adolescent ages (over 16 yrs). Conclusion: These findings suggested that orthodontic treatment of a palatally impacted canine would show good prognosis at an age of early permanent dentition with the canine showing smaller CA and HCV.

Hg₁-xMnxTe산화막의 홀효과에 관한 연구

오재근,최기영,송재흥,김영국,채건식,주유환,설정식,손인호,차성극,이상찬 慶南大學校 附設 基礎科學硏究所 1996 硏究論文集 Vol.8 No.-

HClO₄(pH:2) 수용액에서 양극산화 방법으로 HgMnTe(HMT)의 표면에 산화막을 형성 시켰다. 산화막의 두께는 SEM으로 측정하였으며 10㎛였다. 전류-전압 특성곡선을 얻어 산화 피크 전압으로부터 HTeO₂?, TeO?, HHgO₂? 막이 형성되었음을 알 수 있었다. 산화시키지 않은 HMT와 HClO₄수용액에서 산화시킨 HMT에 대해 Hall전압 및 자기저항을 각각 측정하였다. 홀전압과 자기저항은 HMT보다 HMT산화막에서 더 컸으며 이것은 HHgO₂? 공격자가 홀의 역할을 하고, TeO₄? 산화막은 전하의 포획도를 낮게하기 때문으로 생각할 수 있다. Anodic oxidation processes on HgMnTe surface has been studied in standard aqueous HClO₄(pH:2) solution. The 10㎛ thickness of the anodic oxide layers was measured by SEM. The Composition of the anodic oxide layers are evaluated from current-voltage(I-V) characteristic of HgMnTe Oxidation. The layers are composed of mixed oxide. ??, TeO₄, and?? in HClO₄solution. Transport properties have been investigated in HMT oxide layer made in HClO₄solution and virgin HMT samples at 300K. Hall voltage and magnetoresistance are greater in oxide layer HMT than virgin HMT. As a result, we know that the vacancy of ??in an anodic oxide layer acts as hole and the resulting oxide ?? layer exhibit a reduced degree of charge trapping and increase magnetoresistance.

Chlorpromazine이 백서 해마에서 전기경련 충격에 의한 MAPK 타이로신 인산화에 미치는 영향

주연호,정성훈,전송희,강웅구,김용식,김상욱,박주배 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.2

Object : In order to examine the interaction mechanisms of electroconvulsive shock(ECS) and antipsychotic drug at the level of molecular biology, we observed the effect of chlorpromazine pre-treatment on the activation of mitogen activated protein kinase(MAPK) induced by electroconvulsive shock(ECS) in rat hippocampus Method : Male Sprague-Dawley rats were divided into 2 groups. To the experimental group chlorpromazine(20㎎/㎏) was given intraperitoneally, and to the control distilled water was given instead. Thirty minutes later, ECS was given and the hippocampus was dissected out 2 minutes thereafter. Immunoblotting with antiphosphotyrosine antibody was carried out, and the signal intensity at 42kDa band was quantitized using densitometer. The obtained result was compared by student t-test between the experimental and the control group. The absolute amount of MAPK was measured by immunoblotting with anti-MAPK antibody. Result : The tyrosine phosphorylation of MAPK reached peak at 2 minutes after ECS. However, in the chlorpromazine pre-treated group, the peak level of MAPK tyrosine phosphorylation was significantly attenuated(t=-3.12, df=14, p=0.008) compared to the control. In contrast to this, the absolute amount of MAPK did not differ between the pretreated and the control group. Conclusion : Chlorpromazine attenuated the tyrosine phosphorylation of MAPK by ECS. This finding seems to be related to the fact that chlorpromazine pre-treatment changed the cfos expression by ECS in rat brain. Antipsychotic drug and ECS might interact at the level of MAPK signal transduction system, and this might explained the observed synergistic effect of two treatment modality.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

생체분해성 망막압정을 이용한 망막고정에 대한 실험적 연구

김용백,민병무,김창식,박근성,김승영,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Biodegradable retinal fixation devices obtain mechnical fixation of the retina with desirable chorioretinal scarring and with the potential for local, sustained release of antimetabolites and steroids to inhibit proliferative vitreoretinopathy. We manufactured a biodegradable retinal tack with barb that was designed in order to prevent intrusion from implantation of retinal tacks. This study was carried to evaluate the efficacy for retinal fixation and the capability for sustained release of drugs with a newly designed biodegradable retinal tack Biodegradable retinal tacks were made of polymers of glycolic acids and were designed with barbs in a shape to prevent the disinsertion. Biodegradale retinal tacks are divided into 3 parts, a conical portion that is inserted into the sclera, a cylinder portion that remains in the vitreous, and a neck portion between the pin and the cylinder. The tapered conical end was manufactured to allow easy insertion through the retina and choroid into the sclera. A cylinder portion was manufactured with a tapered angle that fixes firmly into the orifice of 19 gauge spinal needle. A neck portion, 0.4 mm in diameter, was designed to prevent disinsertion from following implantation of retinal tack. The applicator was a 19 gauge spinal needle and its orifice was prepared to 15°angle to accept the tapered cylinder portion of the retinal tack. The retinal tacks, secured in the needles, were passed through the formed vitreous and inserted into the retina, choroid, and sclera and were released by pushing the internal needle, usually within 2-3mm of the medullary ray of the posterior rabbit retina A retinal tack was placed in each of 8 pigmented rabbit eyes. Slit-lamp biomicroscopy, indirect ophthalmoscopy and fundus photography were performed periodically from 1 day to 8 weeks after surgery. Eight eyes were enucleated and studied by light microscopy at 8 weeks. Biomicroscopic evaluation of the animals revealed edemas adjacent to the retinal surfaces immediately after insertion of the biodegradable retinal tacks in all the animals. These edemas disappeared after 1 week. The first noticeable change in the size of retinal tacks was shown after 2weeks. The size of the retinal tacks gradually got smaller, decreasing to about one-half at 4 weeks and about one-third at 8 weeks. All retinal tacks remained in inserted places without any movement for an 8 week period. On light microscopy, epiretinal proliferations were seen to extend into the vitreous cavity. Cellular capsules that lined the inner aspect of the scleral defect caused by tack insertion were found. However the adjacent retina had a normal cytologic appearance and architecture in all specimens. We manufactured a biodegradable retinal tack that is designed to prevent intrusion from implantation of retinal tacks. All biodegradable retinal tacks reduce in size with time, but no retinal tacks extruded from the inserted place. The newly designed biodegradable retinal tack can be used for retinal fixation and may be used as a vehicle for the introduction of pharmacologic agents to prevent the cellular events that promote proliferative vitreoretinopathy.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

RF Modeling of an MOS Varactor and MIM Capacitor in 0.18- ㎛ CMOS Technology

Seong-Sik Song 한국물리학회 2002 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.41 No.6

In this paper, RF models for an MOS varactor and an MIM capacitor fabricated in 0.18-m CMOS technology were developed in the GHz frequency range. In particular, the MOS varactor and the MIM capacitor were modeled in order to predict the behaviors of those with various bias conditions and device dimensions at high frequency, respectively. As a result, the RF models produced results that were in very good agreement with measurements. These models will be very valuable to the RF circuit designers because they can accurately describe the characteristics of MOS varactors and MIM capacitors.